Low genetic diversity and population structuring of Amblyomma hebraeum and Rickettsia africae from coastal and inland regions in the Eastern Cape Province of South Africa
Amblyomma hebraeum is the main vector of Rickettsia africae, the causative agent of African tick bite fever in southern Africa. Because pathogen dispersal is known to be influenced by tick adaptations to climate or host species, this study aimed to analyse the genetic diversity of A. hebraeum and R....
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| Veröffentlicht in: | Medical and veterinary entomology 2023-06, Vol.37 (2), p.275-285 |
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| creator | Pillay, Alicia Nyangiwe, Nkululeko Mukaratirwa, Samson |
| description | Amblyomma hebraeum is the main vector of Rickettsia africae, the causative agent of African tick bite fever in southern Africa. Because pathogen dispersal is known to be influenced by tick adaptations to climate or host species, this study aimed to analyse the genetic diversity of A. hebraeum and R. africae infection of ticks collected from cattle in the Eastern Cape province of South Africa. DNA was extracted, amplified, and sequenced for the COI and ITS2 markers from A. hebraeum samples and the 17 kDa and ompA genes for rickettsial detection. Between six and ten haplotypes were identified from 40 COI and 31 ITS2 sequences; however, no population structuring was observed among sites (ΦST = 0.22, p |
| doi_str_mv | 10.1111/mve.12629 |
| format | Article |
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Haplotype networks from Amblyomma hebraeum populations in the Eastern Cape province of South Africa</description><identifier>ISSN: 0269-283X</identifier><identifier>EISSN: 1365-2915</identifier><identifier>DOI: 10.1111/mve.12629</identifier><identifier>PMID: 36468449</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>African tick-bite fever ; Amblyomma ; Amblyomma hebraeum ; Animals ; Cattle ; Disease transmission ; Genetic analysis ; Genetic diversity ; Genetic Variation ; Haplotypes ; mitochondrial marker ; nuclear marker ; Nucleotide sequence ; Population genetics ; Rickettsia ; Rickettsia africae ; South Africa ; South Africa - epidemiology ; Tick-borne diseases ; Ticks ; tick‐borne disease ; Vector-borne diseases</subject><ispartof>Medical and veterinary entomology, 2023-06, Vol.37 (2), p.275-285</ispartof><rights>2022 The Authors. published by John Wiley & Sons Ltd on behalf of Royal Entomological Society.</rights><rights>2022 The Authors. Medical and Veterinary Entomology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society.</rights><rights>2022. This article is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3489-4d092e5ec854f94045d40de4c8d171fa952cbb4cba01cf94b354d68dcdb346773</cites><orcidid>0000-0002-7872-4709</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fmve.12629$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fmve.12629$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36468449$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pillay, Alicia</creatorcontrib><creatorcontrib>Nyangiwe, Nkululeko</creatorcontrib><creatorcontrib>Mukaratirwa, Samson</creatorcontrib><title>Low genetic diversity and population structuring of Amblyomma hebraeum and Rickettsia africae from coastal and inland regions in the Eastern Cape Province of South Africa</title><title>Medical and veterinary entomology</title><addtitle>Med Vet Entomol</addtitle><description>Amblyomma hebraeum is the main vector of Rickettsia africae, the causative agent of African tick bite fever in southern Africa. Because pathogen dispersal is known to be influenced by tick adaptations to climate or host species, this study aimed to analyse the genetic diversity of A. hebraeum and R. africae infection of ticks collected from cattle in the Eastern Cape province of South Africa. DNA was extracted, amplified, and sequenced for the COI and ITS2 markers from A. hebraeum samples and the 17 kDa and ompA genes for rickettsial detection. Between six and ten haplotypes were identified from 40 COI and 31 ITS2 sequences; however, no population structuring was observed among sites (ΦST = 0.22, p < 0.05). All A. hebraeum isolates clustered with southern Africa GenBank isolates. Rickettsia africae was detected in 46.92% (95% CI = 41%–53%, n = 260) of ticks. All R. africae isolates clustered with strain PELE and Chucks, which were reported previously from South Africa. These results confirm that A. hebraeum populations are undergoing a recent population expansion driven by cattle movement, facilitating local and long dispersal events across the Eastern Cape province.
Haplotype networks from Amblyomma hebraeum populations in the Eastern Cape province of South Africa</description><subject>African tick-bite fever</subject><subject>Amblyomma</subject><subject>Amblyomma hebraeum</subject><subject>Animals</subject><subject>Cattle</subject><subject>Disease transmission</subject><subject>Genetic analysis</subject><subject>Genetic diversity</subject><subject>Genetic Variation</subject><subject>Haplotypes</subject><subject>mitochondrial marker</subject><subject>nuclear marker</subject><subject>Nucleotide sequence</subject><subject>Population genetics</subject><subject>Rickettsia</subject><subject>Rickettsia africae</subject><subject>South Africa</subject><subject>South Africa - epidemiology</subject><subject>Tick-borne diseases</subject><subject>Ticks</subject><subject>tick‐borne disease</subject><subject>Vector-borne diseases</subject><issn>0269-283X</issn><issn>1365-2915</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>EIF</sourceid><recordid>eNp10cuKFDEUBuAgitOOLnwBCbjRRc0kqdQly6ZpL9CieMNdkUpOdWesVGpy6aFfyac03T26EMzmEPjy58CP0HNKrmg-13YPV5TVTDxAC1rWVcEErR6iBWG1KFhb_rhAT0K4IYQ2grHH6KKsed1yLhbo18bd4S1MEI3C2uzBBxMPWE4az25Oo4zGTThEn1RM3kxb7Aa8tP14cNZKvIPeS0j29OCzUT8hxmAkloM3SgIevLNYORmiHE_GTONxeNjm3JCvOO4ArzMAP-GVnAF_8m5vJgXHn764FHd4eUp7ih4Ncgzw7H5eom9v1l9X74rNx7fvV8tNoUreioJrIhhUoNqKD4ITXmlONHDVatrQQYqKqb7nqpeEqgz6suK6brXSfcnrpikv0atz7uzdbYIQO2uCgjEvDi6FjjW8IYS35Ehf_kNvXPJT3q5jLeWsKhsqsnp9Vsq7EDwM3eyNlf7QUdIdC-xygd2pwGxf3Cem3oL-K_80lsH1GdyZEQ7_T-o-fF-fI38Dd7in1g</recordid><startdate>202306</startdate><enddate>202306</enddate><creator>Pillay, Alicia</creator><creator>Nyangiwe, Nkululeko</creator><creator>Mukaratirwa, Samson</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7872-4709</orcidid></search><sort><creationdate>202306</creationdate><title>Low genetic diversity and population structuring of Amblyomma hebraeum and Rickettsia africae from coastal and inland regions in the Eastern Cape Province of South Africa</title><author>Pillay, Alicia ; Nyangiwe, Nkululeko ; Mukaratirwa, Samson</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3489-4d092e5ec854f94045d40de4c8d171fa952cbb4cba01cf94b354d68dcdb346773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>African tick-bite fever</topic><topic>Amblyomma</topic><topic>Amblyomma hebraeum</topic><topic>Animals</topic><topic>Cattle</topic><topic>Disease transmission</topic><topic>Genetic analysis</topic><topic>Genetic diversity</topic><topic>Genetic Variation</topic><topic>Haplotypes</topic><topic>mitochondrial marker</topic><topic>nuclear marker</topic><topic>Nucleotide sequence</topic><topic>Population genetics</topic><topic>Rickettsia</topic><topic>Rickettsia africae</topic><topic>South Africa</topic><topic>South Africa - epidemiology</topic><topic>Tick-borne diseases</topic><topic>Ticks</topic><topic>tick‐borne disease</topic><topic>Vector-borne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pillay, Alicia</creatorcontrib><creatorcontrib>Nyangiwe, Nkululeko</creatorcontrib><creatorcontrib>Mukaratirwa, Samson</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Medical and veterinary entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pillay, Alicia</au><au>Nyangiwe, Nkululeko</au><au>Mukaratirwa, Samson</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low genetic diversity and population structuring of Amblyomma hebraeum and Rickettsia africae from coastal and inland regions in the Eastern Cape Province of South Africa</atitle><jtitle>Medical and veterinary entomology</jtitle><addtitle>Med Vet Entomol</addtitle><date>2023-06</date><risdate>2023</risdate><volume>37</volume><issue>2</issue><spage>275</spage><epage>285</epage><pages>275-285</pages><issn>0269-283X</issn><eissn>1365-2915</eissn><abstract>Amblyomma hebraeum is the main vector of Rickettsia africae, the causative agent of African tick bite fever in southern Africa. Because pathogen dispersal is known to be influenced by tick adaptations to climate or host species, this study aimed to analyse the genetic diversity of A. hebraeum and R. africae infection of ticks collected from cattle in the Eastern Cape province of South Africa. DNA was extracted, amplified, and sequenced for the COI and ITS2 markers from A. hebraeum samples and the 17 kDa and ompA genes for rickettsial detection. Between six and ten haplotypes were identified from 40 COI and 31 ITS2 sequences; however, no population structuring was observed among sites (ΦST = 0.22, p < 0.05). All A. hebraeum isolates clustered with southern Africa GenBank isolates. Rickettsia africae was detected in 46.92% (95% CI = 41%–53%, n = 260) of ticks. All R. africae isolates clustered with strain PELE and Chucks, which were reported previously from South Africa. These results confirm that A. hebraeum populations are undergoing a recent population expansion driven by cattle movement, facilitating local and long dispersal events across the Eastern Cape province.
Haplotype networks from Amblyomma hebraeum populations in the Eastern Cape province of South Africa</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>36468449</pmid><doi>10.1111/mve.12629</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-7872-4709</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | African tick-bite fever Amblyomma Amblyomma hebraeum Animals Cattle Disease transmission Genetic analysis Genetic diversity Genetic Variation Haplotypes mitochondrial marker nuclear marker Nucleotide sequence Population genetics Rickettsia Rickettsia africae South Africa South Africa - epidemiology Tick-borne diseases Ticks tick‐borne disease Vector-borne diseases |
| title | Low genetic diversity and population structuring of Amblyomma hebraeum and Rickettsia africae from coastal and inland regions in the Eastern Cape Province of South Africa |
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