Phosphoenolpyruvate reallocation links nitrogen fixation rates to root nodule energy state
Legume-rhizobium symbiosis in root nodules fixes nitrogen to satisfy the plant's nitrogen demands. The nodules' demand for energy is thought to determine nitrogen fixation rates. How this energy state is sensed to modulate nitrogen fixation is unknown. Here, we identified two soybean ( ) c...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 2022-12, Vol.378 (6623), p.971-977 |
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| creator | Ke, Xiaolong Xiao, Han Peng, Yaqi Wang, Jing Lv, Qi Wang, Xuelu |
| description | Legume-rhizobium symbiosis in root nodules fixes nitrogen to satisfy the plant's nitrogen demands. The nodules' demand for energy is thought to determine nitrogen fixation rates. How this energy state is sensed to modulate nitrogen fixation is unknown. Here, we identified two soybean (
) cystathionine β-synthase domain-containing proteins, nodule AMP sensor 1 (GmNAS1) and NAS1-associated protein 1 (GmNAP1). In the high-nodule energy state, GmNAS1 and GmNAP1 form homodimers that interact with the nuclear factor-Y C (NF-YC) subunit (GmNFYC10a) on mitochondria and reduce its nuclear accumulation. Less nuclear GmNFYC10a leads to lower expression of glycolytic genes involved in pyruvate production, which modulates phosphoenolpyruvate allocation to favor nitrogen fixation. Insight into these pathways may help in the design of leguminous crops that have improved carbon use, nitrogen fixation, and growth. |
| doi_str_mv | 10.1126/science.abq8591 |
| format | Article |
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) cystathionine β-synthase domain-containing proteins, nodule AMP sensor 1 (GmNAS1) and NAS1-associated protein 1 (GmNAP1). In the high-nodule energy state, GmNAS1 and GmNAP1 form homodimers that interact with the nuclear factor-Y C (NF-YC) subunit (GmNFYC10a) on mitochondria and reduce its nuclear accumulation. Less nuclear GmNFYC10a leads to lower expression of glycolytic genes involved in pyruvate production, which modulates phosphoenolpyruvate allocation to favor nitrogen fixation. Insight into these pathways may help in the design of leguminous crops that have improved carbon use, nitrogen fixation, and growth.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.abq8591</identifier><identifier>PMID: 36454840</identifier><language>eng</language><publisher>United States: The American Association for the Advancement of Science</publisher><subject>Cystathionine beta-Synthase ; Energy ; Energy balance ; Glycine max - metabolism ; Glycolysis ; Legumes ; Nitrogen - metabolism ; Nitrogen Fixation ; Nitrogenation ; Nodules ; Phosphoenolpyruvate - metabolism ; Plant Proteins - chemistry ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Protein Domains ; Root nodules ; Root Nodules, Plant - metabolism ; Sensors ; Soybeans</subject><ispartof>Science (American Association for the Advancement of Science), 2022-12, Vol.378 (6623), p.971-977</ispartof><rights>Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c325t-d37a31969b0b9ada115658f5db925b585d217b65efbf777c5b23751b5cf640d13</citedby><cites>FETCH-LOGICAL-c325t-d37a31969b0b9ada115658f5db925b585d217b65efbf777c5b23751b5cf640d13</cites><orcidid>0000-0002-3073-8239 ; 0000-0003-2003-1077</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2884,2885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36454840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ke, Xiaolong</creatorcontrib><creatorcontrib>Xiao, Han</creatorcontrib><creatorcontrib>Peng, Yaqi</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Lv, Qi</creatorcontrib><creatorcontrib>Wang, Xuelu</creatorcontrib><title>Phosphoenolpyruvate reallocation links nitrogen fixation rates to root nodule energy state</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Legume-rhizobium symbiosis in root nodules fixes nitrogen to satisfy the plant's nitrogen demands. The nodules' demand for energy is thought to determine nitrogen fixation rates. How this energy state is sensed to modulate nitrogen fixation is unknown. Here, we identified two soybean (
) cystathionine β-synthase domain-containing proteins, nodule AMP sensor 1 (GmNAS1) and NAS1-associated protein 1 (GmNAP1). In the high-nodule energy state, GmNAS1 and GmNAP1 form homodimers that interact with the nuclear factor-Y C (NF-YC) subunit (GmNFYC10a) on mitochondria and reduce its nuclear accumulation. Less nuclear GmNFYC10a leads to lower expression of glycolytic genes involved in pyruvate production, which modulates phosphoenolpyruvate allocation to favor nitrogen fixation. Insight into these pathways may help in the design of leguminous crops that have improved carbon use, nitrogen fixation, and growth.</description><subject>Cystathionine beta-Synthase</subject><subject>Energy</subject><subject>Energy balance</subject><subject>Glycine max - metabolism</subject><subject>Glycolysis</subject><subject>Legumes</subject><subject>Nitrogen - metabolism</subject><subject>Nitrogen Fixation</subject><subject>Nitrogenation</subject><subject>Nodules</subject><subject>Phosphoenolpyruvate - metabolism</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Protein Domains</subject><subject>Root nodules</subject><subject>Root Nodules, Plant - metabolism</subject><subject>Sensors</subject><subject>Soybeans</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkD1PwzAQhi0EoqUwsyFLLCxp_ZGz4xFVfElIMMDCEtmJ06akdmsniP57UjUwsNxJ9z53Oj0IXVIypZSJWSxq6wo71WabgaJHaEyJgkQxwo_RmBAukoxIGKGzGFeE9Jnip2jERQpplpIx-nhd-rhZeut8s9mF7ku3Fgerm8YXuq29w03tPiN2dRv8wjpc1d-HeejJiFuPg_ctdr7sGouts2Gxw7Htw3N0Uukm2ouhT9D7_d3b_DF5fnl4mt8-JwVn0CYll5pTJZQhRulSUwoCsgpKoxgYyKBkVBoBtjKVlLIAw7gEaqCoREpKyifo5nB3E_y2s7HN13UsbNNoZ30XcyZTwZWAvk7Q9T905bvg-u_2FFDKgcmemh2oIvgYg63yTajXOuxySvK99nzQng_a-42r4W5n1rb843898x_mFIGQ</recordid><startdate>20221202</startdate><enddate>20221202</enddate><creator>Ke, Xiaolong</creator><creator>Xiao, Han</creator><creator>Peng, Yaqi</creator><creator>Wang, Jing</creator><creator>Lv, Qi</creator><creator>Wang, Xuelu</creator><general>The American Association for the Advancement of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3073-8239</orcidid><orcidid>https://orcid.org/0000-0003-2003-1077</orcidid></search><sort><creationdate>20221202</creationdate><title>Phosphoenolpyruvate reallocation links nitrogen fixation rates to root nodule energy state</title><author>Ke, Xiaolong ; 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The nodules' demand for energy is thought to determine nitrogen fixation rates. How this energy state is sensed to modulate nitrogen fixation is unknown. Here, we identified two soybean (
) cystathionine β-synthase domain-containing proteins, nodule AMP sensor 1 (GmNAS1) and NAS1-associated protein 1 (GmNAP1). In the high-nodule energy state, GmNAS1 and GmNAP1 form homodimers that interact with the nuclear factor-Y C (NF-YC) subunit (GmNFYC10a) on mitochondria and reduce its nuclear accumulation. Less nuclear GmNFYC10a leads to lower expression of glycolytic genes involved in pyruvate production, which modulates phosphoenolpyruvate allocation to favor nitrogen fixation. Insight into these pathways may help in the design of leguminous crops that have improved carbon use, nitrogen fixation, and growth.</abstract><cop>United States</cop><pub>The American Association for the Advancement of Science</pub><pmid>36454840</pmid><doi>10.1126/science.abq8591</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-3073-8239</orcidid><orcidid>https://orcid.org/0000-0003-2003-1077</orcidid></addata></record> |
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| subjects | Cystathionine beta-Synthase Energy Energy balance Glycine max - metabolism Glycolysis Legumes Nitrogen - metabolism Nitrogen Fixation Nitrogenation Nodules Phosphoenolpyruvate - metabolism Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Protein Domains Root nodules Root Nodules, Plant - metabolism Sensors Soybeans |
| title | Phosphoenolpyruvate reallocation links nitrogen fixation rates to root nodule energy state |
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