A Hepatocyte Nuclear Factor BtabHNF4 Mediates Desiccation Tolerance and Fecundity in Whitefly (Bemisia tabaci)
Hepatocyte nuclear factor 4 (HNF4) is essential for glucose homeostasis and lipid metabolism in insects. However, little is known about the role of HNF4 in whiteflies. In the present study, we identified a hepatocyte nuclear factor protein from Bemsia tabaci (Diptera: Drosophilidae) and named it Bta...
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| Veröffentlicht in: | Environmental entomology 2023-02, Vol.52 (1), p.138-147 |
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| creator | Wang, Kui Yang, Zhifang Li, Xiang Liu, Shunxiao Wang, Liuhao Zhang, Hongwei Yu, Hao |
| description | Hepatocyte nuclear factor 4 (HNF4) is essential for glucose homeostasis and lipid metabolism in insects. However, little is known about the role of HNF4 in whiteflies. In the present study, we identified a hepatocyte nuclear factor protein from Bemsia tabaci (Diptera: Drosophilidae) and named it BtabHNF4. The full-length of BtabHNF4 was 3,006 bp, encoding a sequence of 434 amino acids that contains a conserved zinc-finger DNA-binding domain (DBD) and a well-conserved ligand-binding domain (LBD). The temporal and spatial expression showed that BtabHNF4 was highly expressed in the female adult stage and abdominal tissues of B. tabaci. A leaf-mediated RNA interference method was used to explore the function of BtabHNF4 in whiteflies. Our results showed that the knockdown of BtabHNF4 influences the desiccation tolerance, egg production, and egg hatching rate of whiteflies. Additionally, BtabHNF4 silencing significantly inhibited the expression level of vitellogenin. These results expand the function of HNF4 and pave the way for understanding the molecular mechanisms of HNF4 in regulating multiple physiological processes. |
| doi_str_mv | 10.1093/ee/nvac103 |
| format | Article |
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However, little is known about the role of HNF4 in whiteflies. In the present study, we identified a hepatocyte nuclear factor protein from Bemsia tabaci (Diptera: Drosophilidae) and named it BtabHNF4. The full-length of BtabHNF4 was 3,006 bp, encoding a sequence of 434 amino acids that contains a conserved zinc-finger DNA-binding domain (DBD) and a well-conserved ligand-binding domain (LBD). The temporal and spatial expression showed that BtabHNF4 was highly expressed in the female adult stage and abdominal tissues of B. tabaci. A leaf-mediated RNA interference method was used to explore the function of BtabHNF4 in whiteflies. Our results showed that the knockdown of BtabHNF4 influences the desiccation tolerance, egg production, and egg hatching rate of whiteflies. Additionally, BtabHNF4 silencing significantly inhibited the expression level of vitellogenin. These results expand the function of HNF4 and pave the way for understanding the molecular mechanisms of HNF4 in regulating multiple physiological processes.</description><identifier>ISSN: 0046-225X</identifier><identifier>EISSN: 1938-2936</identifier><identifier>DOI: 10.1093/ee/nvac103</identifier><identifier>PMID: 36462170</identifier><language>eng</language><publisher>England: Entomological Society of America</publisher><subject>Animals ; Bemisia tabaci ; Desiccation ; desiccation tolerance ; fecundity ; Female ; Fertility ; Hemiptera - genetics ; hepatocyte nuclear factor ; Hepatocyte Nuclear Factors - genetics ; POPULATION GENETICS AND MOLECULAR ECOLOGY ; RNA Interference ; RNAi</subject><ispartof>Environmental entomology, 2023-02, Vol.52 (1), p.138-147</ispartof><rights>The Author(s) 2022. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. journals.permissions@oup.com</rights><rights>The Author(s) 2022. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b320t-919fede22dd0852fa0c0add789abfeabb53748ca3f6db97420db1c2366d9c58a3</citedby><cites>FETCH-LOGICAL-b320t-919fede22dd0852fa0c0add789abfeabb53748ca3f6db97420db1c2366d9c58a3</cites><orcidid>0000-0003-4036-3220</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36462170$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Michel, Andy</contributor><creatorcontrib>Wang, Kui</creatorcontrib><creatorcontrib>Yang, Zhifang</creatorcontrib><creatorcontrib>Li, Xiang</creatorcontrib><creatorcontrib>Liu, Shunxiao</creatorcontrib><creatorcontrib>Wang, Liuhao</creatorcontrib><creatorcontrib>Zhang, Hongwei</creatorcontrib><creatorcontrib>Yu, Hao</creatorcontrib><title>A Hepatocyte Nuclear Factor BtabHNF4 Mediates Desiccation Tolerance and Fecundity in Whitefly (Bemisia tabaci)</title><title>Environmental entomology</title><addtitle>Environ Entomol</addtitle><description>Hepatocyte nuclear factor 4 (HNF4) is essential for glucose homeostasis and lipid metabolism in insects. However, little is known about the role of HNF4 in whiteflies. In the present study, we identified a hepatocyte nuclear factor protein from Bemsia tabaci (Diptera: Drosophilidae) and named it BtabHNF4. The full-length of BtabHNF4 was 3,006 bp, encoding a sequence of 434 amino acids that contains a conserved zinc-finger DNA-binding domain (DBD) and a well-conserved ligand-binding domain (LBD). The temporal and spatial expression showed that BtabHNF4 was highly expressed in the female adult stage and abdominal tissues of B. tabaci. A leaf-mediated RNA interference method was used to explore the function of BtabHNF4 in whiteflies. Our results showed that the knockdown of BtabHNF4 influences the desiccation tolerance, egg production, and egg hatching rate of whiteflies. Additionally, BtabHNF4 silencing significantly inhibited the expression level of vitellogenin. These results expand the function of HNF4 and pave the way for understanding the molecular mechanisms of HNF4 in regulating multiple physiological processes.</description><subject>Animals</subject><subject>Bemisia tabaci</subject><subject>Desiccation</subject><subject>desiccation tolerance</subject><subject>fecundity</subject><subject>Female</subject><subject>Fertility</subject><subject>Hemiptera - genetics</subject><subject>hepatocyte nuclear factor</subject><subject>Hepatocyte Nuclear Factors - genetics</subject><subject>POPULATION GENETICS AND MOLECULAR ECOLOGY</subject><subject>RNA Interference</subject><subject>RNAi</subject><issn>0046-225X</issn><issn>1938-2936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMlOwzAQQC0EgrJc-ADkIyCFekmT-MhWilTgAoJbNLYnwii1S-wg9e8JosCNuczl6c3oEXLI2RlnSo4Rx_4DDGdyg4y4klUmlCw2yYixvMiEmLzskN0Y39gwlSi3yY4s8kLwko2IP6czXEIKZpWQ3vemRejoFEwKHb1IoGf305zeoXWQMNIrjM4YSC54-hha7MAbpOAtnaLpvXVpRZ2nz68uYdOu6PEFLlx0QAcTGHeyT7YaaCMerPceeZpeP17OsvnDze3l-TzTUrCUKa4atCiEtayaiAaYYWBtWSnQDYLWE1nmlQHZFFarMhfMam6ELAqrzKQCuUeOv73LLrz3GFM9vGGwbcFj6GMtyryQKmecD-jpN2q6EGOHTb3s3AK6Vc1Z_dW3RqzXfQf4aO3t9QLtL_oT9O-wdiF4_M_1CXtBhSg</recordid><startdate>20230217</startdate><enddate>20230217</enddate><creator>Wang, Kui</creator><creator>Yang, Zhifang</creator><creator>Li, Xiang</creator><creator>Liu, Shunxiao</creator><creator>Wang, Liuhao</creator><creator>Zhang, Hongwei</creator><creator>Yu, Hao</creator><general>Entomological Society of America</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4036-3220</orcidid></search><sort><creationdate>20230217</creationdate><title>A Hepatocyte Nuclear Factor BtabHNF4 Mediates Desiccation Tolerance and Fecundity in Whitefly (Bemisia tabaci)</title><author>Wang, Kui ; Yang, Zhifang ; Li, Xiang ; Liu, Shunxiao ; Wang, Liuhao ; Zhang, Hongwei ; Yu, Hao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b320t-919fede22dd0852fa0c0add789abfeabb53748ca3f6db97420db1c2366d9c58a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Bemisia tabaci</topic><topic>Desiccation</topic><topic>desiccation tolerance</topic><topic>fecundity</topic><topic>Female</topic><topic>Fertility</topic><topic>Hemiptera - genetics</topic><topic>hepatocyte nuclear factor</topic><topic>Hepatocyte Nuclear Factors - genetics</topic><topic>POPULATION GENETICS AND MOLECULAR ECOLOGY</topic><topic>RNA Interference</topic><topic>RNAi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Kui</creatorcontrib><creatorcontrib>Yang, Zhifang</creatorcontrib><creatorcontrib>Li, Xiang</creatorcontrib><creatorcontrib>Liu, Shunxiao</creatorcontrib><creatorcontrib>Wang, Liuhao</creatorcontrib><creatorcontrib>Zhang, Hongwei</creatorcontrib><creatorcontrib>Yu, Hao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Kui</au><au>Yang, Zhifang</au><au>Li, Xiang</au><au>Liu, Shunxiao</au><au>Wang, Liuhao</au><au>Zhang, Hongwei</au><au>Yu, Hao</au><au>Michel, Andy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Hepatocyte Nuclear Factor BtabHNF4 Mediates Desiccation Tolerance and Fecundity in Whitefly (Bemisia tabaci)</atitle><jtitle>Environmental entomology</jtitle><addtitle>Environ Entomol</addtitle><date>2023-02-17</date><risdate>2023</risdate><volume>52</volume><issue>1</issue><spage>138</spage><epage>147</epage><pages>138-147</pages><issn>0046-225X</issn><eissn>1938-2936</eissn><abstract>Hepatocyte nuclear factor 4 (HNF4) is essential for glucose homeostasis and lipid metabolism in insects. However, little is known about the role of HNF4 in whiteflies. In the present study, we identified a hepatocyte nuclear factor protein from Bemsia tabaci (Diptera: Drosophilidae) and named it BtabHNF4. The full-length of BtabHNF4 was 3,006 bp, encoding a sequence of 434 amino acids that contains a conserved zinc-finger DNA-binding domain (DBD) and a well-conserved ligand-binding domain (LBD). The temporal and spatial expression showed that BtabHNF4 was highly expressed in the female adult stage and abdominal tissues of B. tabaci. A leaf-mediated RNA interference method was used to explore the function of BtabHNF4 in whiteflies. Our results showed that the knockdown of BtabHNF4 influences the desiccation tolerance, egg production, and egg hatching rate of whiteflies. Additionally, BtabHNF4 silencing significantly inhibited the expression level of vitellogenin. These results expand the function of HNF4 and pave the way for understanding the molecular mechanisms of HNF4 in regulating multiple physiological processes.</abstract><cop>England</cop><pub>Entomological Society of America</pub><pmid>36462170</pmid><doi>10.1093/ee/nvac103</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-4036-3220</orcidid></addata></record> |
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| ispartof | Environmental entomology, 2023-02, Vol.52 (1), p.138-147 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Animals Bemisia tabaci Desiccation desiccation tolerance fecundity Female Fertility Hemiptera - genetics hepatocyte nuclear factor Hepatocyte Nuclear Factors - genetics POPULATION GENETICS AND MOLECULAR ECOLOGY RNA Interference RNAi |
| title | A Hepatocyte Nuclear Factor BtabHNF4 Mediates Desiccation Tolerance and Fecundity in Whitefly (Bemisia tabaci) |
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