Biosynthesis of Hybrid Neutral Lipids with Archaeal and Eukaryotic Characteristics in Engineered Saccharomyces cerevisiae
Discovery of the Asgard superphylum of archaea provides new evidence supporting the two‐domain model of life: eukaryotes originated from an Asgard‐related archaeon that engulfed a bacterial endosymbiont. However, how eukaryotes acquired bacterial‐like membrane lipids with a sn‐glycerol‐3‐phosphate (...
Gespeichert in:
| Veröffentlicht in: | Angewandte Chemie International Edition 2023-01, Vol.62 (4), p.e202214344-n/a |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | n/a |
|---|---|
| container_issue | 4 |
| container_start_page | e202214344 |
| container_title | Angewandte Chemie International Edition |
| container_volume | 62 |
| creator | Zhang, Jianzhi Li, Tuo Hong, Zhilai Ma, Chenfei Fang, Xiaoting Zheng, Fengfeng Teng, Wenkai Zhang, Chuanlun Si, Tong |
| description | Discovery of the Asgard superphylum of archaea provides new evidence supporting the two‐domain model of life: eukaryotes originated from an Asgard‐related archaeon that engulfed a bacterial endosymbiont. However, how eukaryotes acquired bacterial‐like membrane lipids with a sn‐glycerol‐3‐phosphate (G3P) backbone instead of the archaeal‐like sn‐glycerol‐1‐phosphate (G1P) backbone remains unknown. In this study, we reconstituted archaeal lipid production in Saccharomyces cerevisiae by expressing unsaturated archaeol‐synthesizing enzymes. Using Golden Gate cloning for pathway assembly, modular gene replacement was performed, revealing the potential biosynthesis of both G1P‐ and G3P‐based unsaturated archaeol by uncultured Asgard archaea. Unexpectedly, hybrid neutral lipids containing both archaeal isoprenoids and eukaryotic fatty acids were observed in recombinant S. cerevisiae. The ability of yeast and archaeal diacylglycerol acyltransferases to synthesize such hybrid lipids was demonstrated.
Reconstitution of archaeal lipid biosynthesis in eukaryotic Saccharomyces cerevisiae resulted in unexpected production of hybrid neutral lipids, which contain both archaeal isoprenoids and eukaryotic fatty acids. The responsible enzymes, diacylglycerol acyltransferases (DGATs), were found to be present in all domains of life. |
| doi_str_mv | 10.1002/anie.202214344 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2740513435</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2740513435</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3734-d4fb998379b723080cf12c40f3e82f142572efd5378de3b67ade06a959b06263</originalsourceid><addsrcrecordid>eNqFkbtPwzAQxi0EgvJYGZElFpYUvxI7Y6nKQ6pggD1y7At1SZNiJ6D89xi1FImF6e4-_e7T6T6EzikZU0LYtW4cjBlhjAouxB4a0ZTRhEvJ92MvOE-kSukROg5hGXmlSHaIjngmmOApG6HhxrVhaLoFBBdwW-H7ofTO4kfoO69rPHdrZwP-dN0CT7xZaIiibiye9W_aD23nDJ4utNemA-9CHAN2DZ41r64B8GDxszZxzberwUDAJmofLjgNp-ig0nWAs209QS-3s5fpfTJ_unuYTuaJ4ZKLxIqqzHPFZV5KxokipqLMCFJxUKyigqWSQWVTLpUFXmZSWyCZztO8JBnL-Am62tiuffveQ-iKlQsG6lo30PahYFKQlPL4jYhe_kGXbe-beFykslTljGUqUuMNZXwbgoeqWHu3ir8oKCm-Mym-Myl2mcSFi61tX67A7vCfECKQb4BPV8Pwj10xeXyY_Zp_AdNnmQc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2765892268</pqid></control><display><type>article</type><title>Biosynthesis of Hybrid Neutral Lipids with Archaeal and Eukaryotic Characteristics in Engineered Saccharomyces cerevisiae</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Zhang, Jianzhi ; Li, Tuo ; Hong, Zhilai ; Ma, Chenfei ; Fang, Xiaoting ; Zheng, Fengfeng ; Teng, Wenkai ; Zhang, Chuanlun ; Si, Tong</creator><creatorcontrib>Zhang, Jianzhi ; Li, Tuo ; Hong, Zhilai ; Ma, Chenfei ; Fang, Xiaoting ; Zheng, Fengfeng ; Teng, Wenkai ; Zhang, Chuanlun ; Si, Tong</creatorcontrib><description>Discovery of the Asgard superphylum of archaea provides new evidence supporting the two‐domain model of life: eukaryotes originated from an Asgard‐related archaeon that engulfed a bacterial endosymbiont. However, how eukaryotes acquired bacterial‐like membrane lipids with a sn‐glycerol‐3‐phosphate (G3P) backbone instead of the archaeal‐like sn‐glycerol‐1‐phosphate (G1P) backbone remains unknown. In this study, we reconstituted archaeal lipid production in Saccharomyces cerevisiae by expressing unsaturated archaeol‐synthesizing enzymes. Using Golden Gate cloning for pathway assembly, modular gene replacement was performed, revealing the potential biosynthesis of both G1P‐ and G3P‐based unsaturated archaeol by uncultured Asgard archaea. Unexpectedly, hybrid neutral lipids containing both archaeal isoprenoids and eukaryotic fatty acids were observed in recombinant S. cerevisiae. The ability of yeast and archaeal diacylglycerol acyltransferases to synthesize such hybrid lipids was demonstrated.
Reconstitution of archaeal lipid biosynthesis in eukaryotic Saccharomyces cerevisiae resulted in unexpected production of hybrid neutral lipids, which contain both archaeal isoprenoids and eukaryotic fatty acids. The responsible enzymes, diacylglycerol acyltransferases (DGATs), were found to be present in all domains of life.</description><edition>International ed. in English</edition><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.202214344</identifier><identifier>PMID: 36424352</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Archaea ; Archaea - genetics ; Bacteria - metabolism ; Biosynthesis ; Chirality ; Cloning ; Diacylglycerol Acyltransferases ; Diglycerides ; Eukaryotes ; Fatty acids ; Glycerol ; Glycerol - metabolism ; Hybrid Neutral Lipids ; Lipids ; Membrane Lipids - metabolism ; Phosphates - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Synthetic Biology ; Terpenes ; Yeasts</subject><ispartof>Angewandte Chemie International Edition, 2023-01, Vol.62 (4), p.e202214344-n/a</ispartof><rights>2022 Wiley‐VCH GmbH</rights><rights>2022 Wiley-VCH GmbH.</rights><rights>2023 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3734-d4fb998379b723080cf12c40f3e82f142572efd5378de3b67ade06a959b06263</citedby><cites>FETCH-LOGICAL-c3734-d4fb998379b723080cf12c40f3e82f142572efd5378de3b67ade06a959b06263</cites><orcidid>0000-0003-2985-9014</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fanie.202214344$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fanie.202214344$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36424352$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Jianzhi</creatorcontrib><creatorcontrib>Li, Tuo</creatorcontrib><creatorcontrib>Hong, Zhilai</creatorcontrib><creatorcontrib>Ma, Chenfei</creatorcontrib><creatorcontrib>Fang, Xiaoting</creatorcontrib><creatorcontrib>Zheng, Fengfeng</creatorcontrib><creatorcontrib>Teng, Wenkai</creatorcontrib><creatorcontrib>Zhang, Chuanlun</creatorcontrib><creatorcontrib>Si, Tong</creatorcontrib><title>Biosynthesis of Hybrid Neutral Lipids with Archaeal and Eukaryotic Characteristics in Engineered Saccharomyces cerevisiae</title><title>Angewandte Chemie International Edition</title><addtitle>Angew Chem Int Ed Engl</addtitle><description>Discovery of the Asgard superphylum of archaea provides new evidence supporting the two‐domain model of life: eukaryotes originated from an Asgard‐related archaeon that engulfed a bacterial endosymbiont. However, how eukaryotes acquired bacterial‐like membrane lipids with a sn‐glycerol‐3‐phosphate (G3P) backbone instead of the archaeal‐like sn‐glycerol‐1‐phosphate (G1P) backbone remains unknown. In this study, we reconstituted archaeal lipid production in Saccharomyces cerevisiae by expressing unsaturated archaeol‐synthesizing enzymes. Using Golden Gate cloning for pathway assembly, modular gene replacement was performed, revealing the potential biosynthesis of both G1P‐ and G3P‐based unsaturated archaeol by uncultured Asgard archaea. Unexpectedly, hybrid neutral lipids containing both archaeal isoprenoids and eukaryotic fatty acids were observed in recombinant S. cerevisiae. The ability of yeast and archaeal diacylglycerol acyltransferases to synthesize such hybrid lipids was demonstrated.
Reconstitution of archaeal lipid biosynthesis in eukaryotic Saccharomyces cerevisiae resulted in unexpected production of hybrid neutral lipids, which contain both archaeal isoprenoids and eukaryotic fatty acids. The responsible enzymes, diacylglycerol acyltransferases (DGATs), were found to be present in all domains of life.</description><subject>Archaea</subject><subject>Archaea - genetics</subject><subject>Bacteria - metabolism</subject><subject>Biosynthesis</subject><subject>Chirality</subject><subject>Cloning</subject><subject>Diacylglycerol Acyltransferases</subject><subject>Diglycerides</subject><subject>Eukaryotes</subject><subject>Fatty acids</subject><subject>Glycerol</subject><subject>Glycerol - metabolism</subject><subject>Hybrid Neutral Lipids</subject><subject>Lipids</subject><subject>Membrane Lipids - metabolism</subject><subject>Phosphates - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Synthetic Biology</subject><subject>Terpenes</subject><subject>Yeasts</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbtPwzAQxi0EgvJYGZElFpYUvxI7Y6nKQ6pggD1y7At1SZNiJ6D89xi1FImF6e4-_e7T6T6EzikZU0LYtW4cjBlhjAouxB4a0ZTRhEvJ92MvOE-kSukROg5hGXmlSHaIjngmmOApG6HhxrVhaLoFBBdwW-H7ofTO4kfoO69rPHdrZwP-dN0CT7xZaIiibiye9W_aD23nDJ4utNemA-9CHAN2DZ41r64B8GDxszZxzberwUDAJmofLjgNp-ig0nWAs209QS-3s5fpfTJ_unuYTuaJ4ZKLxIqqzHPFZV5KxokipqLMCFJxUKyigqWSQWVTLpUFXmZSWyCZztO8JBnL-Am62tiuffveQ-iKlQsG6lo30PahYFKQlPL4jYhe_kGXbe-beFykslTljGUqUuMNZXwbgoeqWHu3ir8oKCm-Mym-Myl2mcSFi61tX67A7vCfECKQb4BPV8Pwj10xeXyY_Zp_AdNnmQc</recordid><startdate>20230123</startdate><enddate>20230123</enddate><creator>Zhang, Jianzhi</creator><creator>Li, Tuo</creator><creator>Hong, Zhilai</creator><creator>Ma, Chenfei</creator><creator>Fang, Xiaoting</creator><creator>Zheng, Fengfeng</creator><creator>Teng, Wenkai</creator><creator>Zhang, Chuanlun</creator><creator>Si, Tong</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2985-9014</orcidid></search><sort><creationdate>20230123</creationdate><title>Biosynthesis of Hybrid Neutral Lipids with Archaeal and Eukaryotic Characteristics in Engineered Saccharomyces cerevisiae</title><author>Zhang, Jianzhi ; Li, Tuo ; Hong, Zhilai ; Ma, Chenfei ; Fang, Xiaoting ; Zheng, Fengfeng ; Teng, Wenkai ; Zhang, Chuanlun ; Si, Tong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3734-d4fb998379b723080cf12c40f3e82f142572efd5378de3b67ade06a959b06263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Archaea</topic><topic>Archaea - genetics</topic><topic>Bacteria - metabolism</topic><topic>Biosynthesis</topic><topic>Chirality</topic><topic>Cloning</topic><topic>Diacylglycerol Acyltransferases</topic><topic>Diglycerides</topic><topic>Eukaryotes</topic><topic>Fatty acids</topic><topic>Glycerol</topic><topic>Glycerol - metabolism</topic><topic>Hybrid Neutral Lipids</topic><topic>Lipids</topic><topic>Membrane Lipids - metabolism</topic><topic>Phosphates - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Synthetic Biology</topic><topic>Terpenes</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Jianzhi</creatorcontrib><creatorcontrib>Li, Tuo</creatorcontrib><creatorcontrib>Hong, Zhilai</creatorcontrib><creatorcontrib>Ma, Chenfei</creatorcontrib><creatorcontrib>Fang, Xiaoting</creatorcontrib><creatorcontrib>Zheng, Fengfeng</creatorcontrib><creatorcontrib>Teng, Wenkai</creatorcontrib><creatorcontrib>Zhang, Chuanlun</creatorcontrib><creatorcontrib>Si, Tong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Jianzhi</au><au>Li, Tuo</au><au>Hong, Zhilai</au><au>Ma, Chenfei</au><au>Fang, Xiaoting</au><au>Zheng, Fengfeng</au><au>Teng, Wenkai</au><au>Zhang, Chuanlun</au><au>Si, Tong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biosynthesis of Hybrid Neutral Lipids with Archaeal and Eukaryotic Characteristics in Engineered Saccharomyces cerevisiae</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angew Chem Int Ed Engl</addtitle><date>2023-01-23</date><risdate>2023</risdate><volume>62</volume><issue>4</issue><spage>e202214344</spage><epage>n/a</epage><pages>e202214344-n/a</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><abstract>Discovery of the Asgard superphylum of archaea provides new evidence supporting the two‐domain model of life: eukaryotes originated from an Asgard‐related archaeon that engulfed a bacterial endosymbiont. However, how eukaryotes acquired bacterial‐like membrane lipids with a sn‐glycerol‐3‐phosphate (G3P) backbone instead of the archaeal‐like sn‐glycerol‐1‐phosphate (G1P) backbone remains unknown. In this study, we reconstituted archaeal lipid production in Saccharomyces cerevisiae by expressing unsaturated archaeol‐synthesizing enzymes. Using Golden Gate cloning for pathway assembly, modular gene replacement was performed, revealing the potential biosynthesis of both G1P‐ and G3P‐based unsaturated archaeol by uncultured Asgard archaea. Unexpectedly, hybrid neutral lipids containing both archaeal isoprenoids and eukaryotic fatty acids were observed in recombinant S. cerevisiae. The ability of yeast and archaeal diacylglycerol acyltransferases to synthesize such hybrid lipids was demonstrated.
Reconstitution of archaeal lipid biosynthesis in eukaryotic Saccharomyces cerevisiae resulted in unexpected production of hybrid neutral lipids, which contain both archaeal isoprenoids and eukaryotic fatty acids. The responsible enzymes, diacylglycerol acyltransferases (DGATs), were found to be present in all domains of life.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36424352</pmid><doi>10.1002/anie.202214344</doi><tpages>9</tpages><edition>International ed. in English</edition><orcidid>https://orcid.org/0000-0003-2985-9014</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1433-7851 |
| ispartof | Angewandte Chemie International Edition, 2023-01, Vol.62 (4), p.e202214344-n/a |
| issn | 1433-7851 1521-3773 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2740513435 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Archaea Archaea - genetics Bacteria - metabolism Biosynthesis Chirality Cloning Diacylglycerol Acyltransferases Diglycerides Eukaryotes Fatty acids Glycerol Glycerol - metabolism Hybrid Neutral Lipids Lipids Membrane Lipids - metabolism Phosphates - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Synthetic Biology Terpenes Yeasts |
| title | Biosynthesis of Hybrid Neutral Lipids with Archaeal and Eukaryotic Characteristics in Engineered Saccharomyces cerevisiae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T23%3A56%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biosynthesis%20of%20Hybrid%20Neutral%20Lipids%20with%20Archaeal%20and%20Eukaryotic%20Characteristics%20in%20Engineered%20Saccharomyces%20cerevisiae&rft.jtitle=Angewandte%20Chemie%20International%20Edition&rft.au=Zhang,%20Jianzhi&rft.date=2023-01-23&rft.volume=62&rft.issue=4&rft.spage=e202214344&rft.epage=n/a&rft.pages=e202214344-n/a&rft.issn=1433-7851&rft.eissn=1521-3773&rft_id=info:doi/10.1002/anie.202214344&rft_dat=%3Cproquest_cross%3E2740513435%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2765892268&rft_id=info:pmid/36424352&rfr_iscdi=true |