Development, characterization and probiotic encapsulating ability of novel Momordica charantia bioactive polysaccharides/whey protein isolate composite gels
In this study, a polysaccharide (MP1) with a molecular weight of 38 kDa was isolated from Momordica charantia which contains arabinose, galactose, xylose, and rhamnose. (MP1) was used to formulate composite gels with Whey Protein Isolate (WPI) that were characterized for their functional properties,...
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| Veröffentlicht in: | International journal of biological macromolecules 2023-01, Vol.225, p.454-466 |
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| creator | Bora, Awa Fanny Massounga Kouame, Kouadio Jean Eric-Parfait Li, Xiaodong Liu, Lu Sun, Yue Ma, Qian Liu, Yibo |
| description | In this study, a polysaccharide (MP1) with a molecular weight of 38 kDa was isolated from Momordica charantia which contains arabinose, galactose, xylose, and rhamnose. (MP1) was used to formulate composite gels with Whey Protein Isolate (WPI) that were characterized for their functional properties, microstructure, thermal resistance, probiotic encapsulating ability, and potential toward metabolic syndrome (MS). Results showed that the highest complex index was obtained at MP concentration of 2 %. MP-WPIs demonstrated superior (p |
| doi_str_mv | 10.1016/j.ijbiomac.2022.11.097 |
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•Novel composite gels were produced by heat-induced gelation of WPI and MP.•MP-WPIs had higher WHC and emulsifying properties than WPI.•MP addition increased WPI gels strength and thermal stability.•MP-WPIs provided superior protection to LA upon freeze-drying and in vitro digestion, compared to WPI.•LA-MP-WPI showed to be a promising therapeutic candidate for MS.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2022.11.097</identifier><identifier>PMID: 36410535</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>arabinose ; Composite gels ; digestion ; encapsulation ; freeze drying ; galactose ; Gels - chemistry ; heat tolerance ; hydrogen ; Hydrogen-Ion Concentration ; hydrophobicity ; Lactobacillus acidophilus ; metabolic syndrome ; microstructure ; molecular weight ; Momordica charantia ; Momordica charantia polysaccharides ; polysaccharides ; Polysaccharides - chemistry ; prebiotics ; probiotics ; Probiotics - chemistry ; protein structure ; rhamnose ; storage quality ; thermal stability ; Whey protein isolate ; Whey Proteins - chemistry ; xylose</subject><ispartof>International journal of biological macromolecules, 2023-01, Vol.225, p.454-466</ispartof><rights>2022 Elsevier B.V.</rights><rights>Copyright © 2022 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-262d9377ddc5714ee1fcde7eadb6c39313d5dba842dd3cec52dbc7d68aa87d923</citedby><cites>FETCH-LOGICAL-c401t-262d9377ddc5714ee1fcde7eadb6c39313d5dba842dd3cec52dbc7d68aa87d923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2022.11.097$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36410535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bora, Awa Fanny Massounga</creatorcontrib><creatorcontrib>Kouame, Kouadio Jean Eric-Parfait</creatorcontrib><creatorcontrib>Li, Xiaodong</creatorcontrib><creatorcontrib>Liu, Lu</creatorcontrib><creatorcontrib>Sun, Yue</creatorcontrib><creatorcontrib>Ma, Qian</creatorcontrib><creatorcontrib>Liu, Yibo</creatorcontrib><title>Development, characterization and probiotic encapsulating ability of novel Momordica charantia bioactive polysaccharides/whey protein isolate composite gels</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>In this study, a polysaccharide (MP1) with a molecular weight of 38 kDa was isolated from Momordica charantia which contains arabinose, galactose, xylose, and rhamnose. (MP1) was used to formulate composite gels with Whey Protein Isolate (WPI) that were characterized for their functional properties, microstructure, thermal resistance, probiotic encapsulating ability, and potential toward metabolic syndrome (MS). Results showed that the highest complex index was obtained at MP concentration of 2 %. MP-WPIs demonstrated superior (p < 0.05) water holding capacity and emulsifying properties than WPI gels. MP-WPIs also had higher (p < 0.05) thermal stability via TGA and DSC analysis. MP-WPI morphology was observed via SEM whereas protein structure as affected by MP concentration was studied using CLSM. Also, FTIR revealed that MP and WPI bonded mainly through electrostatic, hydrophobic and hydrogen interactions. More, MP-WPIs successfully enhanced probiotic Lactobacillus acidophilus (LA) survival upon freeze-drying with high encapsulation efficiency (98 %) and improved storage stability. MP-WPIs improved LA survival upon digestion suggesting a potential prebiotic activity. Finally, synbiotic formulation LA-MP-WPIs exhibited effective biological activity against MS. Therefore, MP-WPIs is a propitious strategy for effective probiotic gastrointestinal delivery with potential toward MS.
•Novel composite gels were produced by heat-induced gelation of WPI and MP.•MP-WPIs had higher WHC and emulsifying properties than WPI.•MP addition increased WPI gels strength and thermal stability.•MP-WPIs provided superior protection to LA upon freeze-drying and in vitro digestion, compared to WPI.•LA-MP-WPI showed to be a promising therapeutic candidate for MS.</description><subject>arabinose</subject><subject>Composite gels</subject><subject>digestion</subject><subject>encapsulation</subject><subject>freeze drying</subject><subject>galactose</subject><subject>Gels - chemistry</subject><subject>heat tolerance</subject><subject>hydrogen</subject><subject>Hydrogen-Ion Concentration</subject><subject>hydrophobicity</subject><subject>Lactobacillus acidophilus</subject><subject>metabolic syndrome</subject><subject>microstructure</subject><subject>molecular weight</subject><subject>Momordica charantia</subject><subject>Momordica charantia polysaccharides</subject><subject>polysaccharides</subject><subject>Polysaccharides - chemistry</subject><subject>prebiotics</subject><subject>probiotics</subject><subject>Probiotics - chemistry</subject><subject>protein structure</subject><subject>rhamnose</subject><subject>storage quality</subject><subject>thermal stability</subject><subject>Whey protein isolate</subject><subject>Whey Proteins - chemistry</subject><subject>xylose</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EotvCX6h85EBSj53EyQ1UoCAVcYGz5diz7aySONjZrZbfwo_F0bZce7Llee97Iz_GLkGUIKC52pW06ymM1pVSSFkClKLTL9gGWt0VQgj1km0EVFC0oMQZO09pl1-bGtrX7Ew1FYha1Rv29xMecAjziNPynrt7G61bMNIfu1CYuJ08n2PISQs5jpOzc9oPeTbdcdvTQMuRhy2fQobw72EM0ZOzJ860kOXZmYF0QD6H4ZisW0fkMV093ONxZS9IE6cUMhW5C-McEuXbHQ7pDXu1tUPCt4_nBfv15fPP66_F7Y-bb9cfbwtXCVgK2UjfKa29d7WGChG2zqNG6_vGqU6B8rXvbVtJ75VDV0vfO-2b1tpW-06qC_buxM3r_N5jWsxIyeEw2AnDPhkFtWoBdAXPSqVWnWg6KVdqc5K6GFKKuDVzpNHGowFh1hLNzjyVaNYSDYDJJWbj5WPGvh_R_7c9tZYFH06C_Ed4IIwmOcrloKeIbjE-0HMZ_wBUxbbf</recordid><startdate>20230115</startdate><enddate>20230115</enddate><creator>Bora, Awa Fanny Massounga</creator><creator>Kouame, Kouadio Jean Eric-Parfait</creator><creator>Li, Xiaodong</creator><creator>Liu, Lu</creator><creator>Sun, Yue</creator><creator>Ma, Qian</creator><creator>Liu, Yibo</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20230115</creationdate><title>Development, characterization and probiotic encapsulating ability of novel Momordica charantia bioactive polysaccharides/whey protein isolate composite gels</title><author>Bora, Awa Fanny Massounga ; Kouame, Kouadio Jean Eric-Parfait ; Li, Xiaodong ; Liu, Lu ; Sun, Yue ; Ma, Qian ; Liu, Yibo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-262d9377ddc5714ee1fcde7eadb6c39313d5dba842dd3cec52dbc7d68aa87d923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>arabinose</topic><topic>Composite gels</topic><topic>digestion</topic><topic>encapsulation</topic><topic>freeze drying</topic><topic>galactose</topic><topic>Gels - chemistry</topic><topic>heat tolerance</topic><topic>hydrogen</topic><topic>Hydrogen-Ion Concentration</topic><topic>hydrophobicity</topic><topic>Lactobacillus acidophilus</topic><topic>metabolic syndrome</topic><topic>microstructure</topic><topic>molecular weight</topic><topic>Momordica charantia</topic><topic>Momordica charantia polysaccharides</topic><topic>polysaccharides</topic><topic>Polysaccharides - chemistry</topic><topic>prebiotics</topic><topic>probiotics</topic><topic>Probiotics - chemistry</topic><topic>protein structure</topic><topic>rhamnose</topic><topic>storage quality</topic><topic>thermal stability</topic><topic>Whey protein isolate</topic><topic>Whey Proteins - chemistry</topic><topic>xylose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bora, Awa Fanny Massounga</creatorcontrib><creatorcontrib>Kouame, Kouadio Jean Eric-Parfait</creatorcontrib><creatorcontrib>Li, Xiaodong</creatorcontrib><creatorcontrib>Liu, Lu</creatorcontrib><creatorcontrib>Sun, Yue</creatorcontrib><creatorcontrib>Ma, Qian</creatorcontrib><creatorcontrib>Liu, Yibo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bora, Awa Fanny Massounga</au><au>Kouame, Kouadio Jean Eric-Parfait</au><au>Li, Xiaodong</au><au>Liu, Lu</au><au>Sun, Yue</au><au>Ma, Qian</au><au>Liu, Yibo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development, characterization and probiotic encapsulating ability of novel Momordica charantia bioactive polysaccharides/whey protein isolate composite gels</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2023-01-15</date><risdate>2023</risdate><volume>225</volume><spage>454</spage><epage>466</epage><pages>454-466</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>In this study, a polysaccharide (MP1) with a molecular weight of 38 kDa was isolated from Momordica charantia which contains arabinose, galactose, xylose, and rhamnose. (MP1) was used to formulate composite gels with Whey Protein Isolate (WPI) that were characterized for their functional properties, microstructure, thermal resistance, probiotic encapsulating ability, and potential toward metabolic syndrome (MS). Results showed that the highest complex index was obtained at MP concentration of 2 %. MP-WPIs demonstrated superior (p < 0.05) water holding capacity and emulsifying properties than WPI gels. MP-WPIs also had higher (p < 0.05) thermal stability via TGA and DSC analysis. MP-WPI morphology was observed via SEM whereas protein structure as affected by MP concentration was studied using CLSM. Also, FTIR revealed that MP and WPI bonded mainly through electrostatic, hydrophobic and hydrogen interactions. More, MP-WPIs successfully enhanced probiotic Lactobacillus acidophilus (LA) survival upon freeze-drying with high encapsulation efficiency (98 %) and improved storage stability. MP-WPIs improved LA survival upon digestion suggesting a potential prebiotic activity. Finally, synbiotic formulation LA-MP-WPIs exhibited effective biological activity against MS. Therefore, MP-WPIs is a propitious strategy for effective probiotic gastrointestinal delivery with potential toward MS.
•Novel composite gels were produced by heat-induced gelation of WPI and MP.•MP-WPIs had higher WHC and emulsifying properties than WPI.•MP addition increased WPI gels strength and thermal stability.•MP-WPIs provided superior protection to LA upon freeze-drying and in vitro digestion, compared to WPI.•LA-MP-WPI showed to be a promising therapeutic candidate for MS.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36410535</pmid><doi>10.1016/j.ijbiomac.2022.11.097</doi><tpages>13</tpages></addata></record> |
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| subjects | arabinose Composite gels digestion encapsulation freeze drying galactose Gels - chemistry heat tolerance hydrogen Hydrogen-Ion Concentration hydrophobicity Lactobacillus acidophilus metabolic syndrome microstructure molecular weight Momordica charantia Momordica charantia polysaccharides polysaccharides Polysaccharides - chemistry prebiotics probiotics Probiotics - chemistry protein structure rhamnose storage quality thermal stability Whey protein isolate Whey Proteins - chemistry xylose |
| title | Development, characterization and probiotic encapsulating ability of novel Momordica charantia bioactive polysaccharides/whey protein isolate composite gels |
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