EF‐24 inhibits TPA‐induced cellular migration and MMP‐9 expression through the p38 signaling pathway in cervical cancer cells
Diphenyl difluoroketone (EF‐24), a synthetic curcumin analog, has enhanced bioavailability over curcumin. EF‐24 acts more powerful bioactivity for anti‐inflammatory and anti‐cancer activity. However, the effects and mechanism of EF‐24 on cervical cancer has not been fully investigated. Herein, this...
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| Veröffentlicht in: | Environmental toxicology 2023-02, Vol.38 (2), p.451-459 |
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| container_title | Environmental toxicology |
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| creator | Lee, Chung‐Yuan Ho, Yung‐Chuan Lin, Chiao‐Wen Hsin, Min‐Chieh Wang, Po‐Hui Tang, Ya‐Cheng Yang, Shun‐Fa Hsiao, Yi‐Hsuan |
| description | Diphenyl difluoroketone (EF‐24), a synthetic curcumin analog, has enhanced bioavailability over curcumin. EF‐24 acts more powerful bioactivity for anti‐inflammatory and anti‐cancer activity. However, the effects and mechanism of EF‐24 on cervical cancer has not been fully investigated. Herein, this study evaluated the effects of EF‐24 on TPA‐induced cellular migration of cervical cancer. The results showed that EF‐24 substantially reduced the cellular migration and cellular invasion of the HeLa and SiHa cells. Moreover, gelatin zymography, western blotting analyses and real‐time PCR revealed that EF‐24 suppressed Matrix metalloproteinase‐9 (MMP‐9) activity, protein expression and mRNA levels. Mechanistically, EF‐24 inhibited the phosphorylation of the p38 signaling pathway. In conclusion, EF‐24 inhibited TPA‐induced cellular migration and cellular invasion of cervical cancer cell lines through modulating MMP‐9 expression via downregulating signaling p38 pathway and EF‐24 may have potential to serve as a chemopreventive agent of cervical cancer. |
| doi_str_mv | 10.1002/tox.23709 |
| format | Article |
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EF‐24 acts more powerful bioactivity for anti‐inflammatory and anti‐cancer activity. However, the effects and mechanism of EF‐24 on cervical cancer has not been fully investigated. Herein, this study evaluated the effects of EF‐24 on TPA‐induced cellular migration of cervical cancer. The results showed that EF‐24 substantially reduced the cellular migration and cellular invasion of the HeLa and SiHa cells. Moreover, gelatin zymography, western blotting analyses and real‐time PCR revealed that EF‐24 suppressed Matrix metalloproteinase‐9 (MMP‐9) activity, protein expression and mRNA levels. Mechanistically, EF‐24 inhibited the phosphorylation of the p38 signaling pathway. In conclusion, EF‐24 inhibited TPA‐induced cellular migration and cellular invasion of cervical cancer cell lines through modulating MMP‐9 expression via downregulating signaling p38 pathway and EF‐24 may have potential to serve as a chemopreventive agent of cervical cancer.</description><identifier>ISSN: 1520-4081</identifier><identifier>EISSN: 1522-7278</identifier><identifier>DOI: 10.1002/tox.23709</identifier><identifier>PMID: 36413041</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Bioavailability ; Biological activity ; Cancer ; Cell Line, Tumor ; Cell lines ; Cell Movement - drug effects ; Cells ; Cervical cancer ; Cervix ; Curcumin ; Curcumin - analogs & derivatives ; Curcumin - pharmacology ; EF‐24 ; Female ; Gelatin ; Gene expression ; Humans ; Inflammation ; invasion ; Matrix metalloproteinase ; Matrix Metalloproteinase 9 - genetics ; Matrix Metalloproteinase 9 - metabolism ; Matrix metalloproteinases ; Metalloproteinase ; migration ; MMP‐9 ; mRNA ; Neoplasm Invasiveness ; Nucleotide sequence ; Phosphorylation ; Signal Transduction ; Signaling ; Tumor cell lines ; Uterine Cervical Neoplasms - enzymology ; Uterine Cervical Neoplasms - pathology ; Western blotting</subject><ispartof>Environmental toxicology, 2023-02, Vol.38 (2), p.451-459</ispartof><rights>2022 Wiley Periodicals LLC.</rights><rights>2023 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3539-2a14cef068b0740a23a7dd2e8822cb3ab50ca47da25f1b9aa5373db9fc1503053</citedby><cites>FETCH-LOGICAL-c3539-2a14cef068b0740a23a7dd2e8822cb3ab50ca47da25f1b9aa5373db9fc1503053</cites><orcidid>0000-0002-0365-7927</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Ftox.23709$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Ftox.23709$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36413041$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Chung‐Yuan</creatorcontrib><creatorcontrib>Ho, Yung‐Chuan</creatorcontrib><creatorcontrib>Lin, Chiao‐Wen</creatorcontrib><creatorcontrib>Hsin, Min‐Chieh</creatorcontrib><creatorcontrib>Wang, Po‐Hui</creatorcontrib><creatorcontrib>Tang, Ya‐Cheng</creatorcontrib><creatorcontrib>Yang, Shun‐Fa</creatorcontrib><creatorcontrib>Hsiao, Yi‐Hsuan</creatorcontrib><title>EF‐24 inhibits TPA‐induced cellular migration and MMP‐9 expression through the p38 signaling pathway in cervical cancer cells</title><title>Environmental toxicology</title><addtitle>Environ Toxicol</addtitle><description>Diphenyl difluoroketone (EF‐24), a synthetic curcumin analog, has enhanced bioavailability over curcumin. EF‐24 acts more powerful bioactivity for anti‐inflammatory and anti‐cancer activity. However, the effects and mechanism of EF‐24 on cervical cancer has not been fully investigated. Herein, this study evaluated the effects of EF‐24 on TPA‐induced cellular migration of cervical cancer. The results showed that EF‐24 substantially reduced the cellular migration and cellular invasion of the HeLa and SiHa cells. Moreover, gelatin zymography, western blotting analyses and real‐time PCR revealed that EF‐24 suppressed Matrix metalloproteinase‐9 (MMP‐9) activity, protein expression and mRNA levels. Mechanistically, EF‐24 inhibited the phosphorylation of the p38 signaling pathway. In conclusion, EF‐24 inhibited TPA‐induced cellular migration and cellular invasion of cervical cancer cell lines through modulating MMP‐9 expression via downregulating signaling p38 pathway and EF‐24 may have potential to serve as a chemopreventive agent of cervical cancer.</description><subject>Bioavailability</subject><subject>Biological activity</subject><subject>Cancer</subject><subject>Cell Line, Tumor</subject><subject>Cell lines</subject><subject>Cell Movement - drug effects</subject><subject>Cells</subject><subject>Cervical cancer</subject><subject>Cervix</subject><subject>Curcumin</subject><subject>Curcumin - analogs & derivatives</subject><subject>Curcumin - pharmacology</subject><subject>EF‐24</subject><subject>Female</subject><subject>Gelatin</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Inflammation</subject><subject>invasion</subject><subject>Matrix metalloproteinase</subject><subject>Matrix Metalloproteinase 9 - genetics</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>Matrix metalloproteinases</subject><subject>Metalloproteinase</subject><subject>migration</subject><subject>MMP‐9</subject><subject>mRNA</subject><subject>Neoplasm Invasiveness</subject><subject>Nucleotide sequence</subject><subject>Phosphorylation</subject><subject>Signal Transduction</subject><subject>Signaling</subject><subject>Tumor cell lines</subject><subject>Uterine Cervical Neoplasms - enzymology</subject><subject>Uterine Cervical Neoplasms - pathology</subject><subject>Western blotting</subject><issn>1520-4081</issn><issn>1522-7278</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc9O3DAQh62qFcu_Q1-gstRLOQTGdhwnR4QWigSCwyJxiyaOs-tVNkntpMvekHgBnrFPgrMLPVTqaUajT9_M6EfIVwanDICf9e3TKRcKsk9kn0nOI8VV-nnbQxRDyibkwPslAGSJTPbIRCQxExCzffIyvfzz_MpjapuFLWzv6ez-PExsUw7alFSbuh5qdHRl5w572zYUm5Le3t4HKKPmqXPG-3HcL1w7zBehGtqJlHo7b7C2zZx22C_WuAkrgs79thprqrEJ_Vbvj8iXCmtvjt_rIXm4nM4ufkY3d1fXF-c3kRZSZBFHFmtTQZIWoGJALlCVJTdpyrkuBBYSNMaqRC4rVmSIUihRFlmlmQQBUhySHztv59pfg_F9vrJ-vAAb0w4-50pkkCgueUC__4Mu28GFf0YqkTyLFRuFJztKu9Z7Z6q8c3aFbpMzyMdk8pBMvk0msN_ejUOxMuVf8iOKAJztgLWtzeb_pnx297hTvgGqTZp_</recordid><startdate>202302</startdate><enddate>202302</enddate><creator>Lee, Chung‐Yuan</creator><creator>Ho, Yung‐Chuan</creator><creator>Lin, Chiao‐Wen</creator><creator>Hsin, Min‐Chieh</creator><creator>Wang, Po‐Hui</creator><creator>Tang, Ya‐Cheng</creator><creator>Yang, Shun‐Fa</creator><creator>Hsiao, Yi‐Hsuan</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7ST</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M7N</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0365-7927</orcidid></search><sort><creationdate>202302</creationdate><title>EF‐24 inhibits TPA‐induced cellular migration and MMP‐9 expression through the p38 signaling pathway in cervical cancer cells</title><author>Lee, Chung‐Yuan ; Ho, Yung‐Chuan ; Lin, Chiao‐Wen ; Hsin, Min‐Chieh ; Wang, Po‐Hui ; Tang, Ya‐Cheng ; Yang, Shun‐Fa ; Hsiao, Yi‐Hsuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3539-2a14cef068b0740a23a7dd2e8822cb3ab50ca47da25f1b9aa5373db9fc1503053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Bioavailability</topic><topic>Biological activity</topic><topic>Cancer</topic><topic>Cell Line, Tumor</topic><topic>Cell lines</topic><topic>Cell Movement - drug effects</topic><topic>Cells</topic><topic>Cervical cancer</topic><topic>Cervix</topic><topic>Curcumin</topic><topic>Curcumin - analogs & derivatives</topic><topic>Curcumin - pharmacology</topic><topic>EF‐24</topic><topic>Female</topic><topic>Gelatin</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Inflammation</topic><topic>invasion</topic><topic>Matrix metalloproteinase</topic><topic>Matrix Metalloproteinase 9 - genetics</topic><topic>Matrix Metalloproteinase 9 - metabolism</topic><topic>Matrix metalloproteinases</topic><topic>Metalloproteinase</topic><topic>migration</topic><topic>MMP‐9</topic><topic>mRNA</topic><topic>Neoplasm Invasiveness</topic><topic>Nucleotide sequence</topic><topic>Phosphorylation</topic><topic>Signal Transduction</topic><topic>Signaling</topic><topic>Tumor cell lines</topic><topic>Uterine Cervical Neoplasms - enzymology</topic><topic>Uterine Cervical Neoplasms - pathology</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Chung‐Yuan</creatorcontrib><creatorcontrib>Ho, Yung‐Chuan</creatorcontrib><creatorcontrib>Lin, Chiao‐Wen</creatorcontrib><creatorcontrib>Hsin, Min‐Chieh</creatorcontrib><creatorcontrib>Wang, Po‐Hui</creatorcontrib><creatorcontrib>Tang, Ya‐Cheng</creatorcontrib><creatorcontrib>Yang, Shun‐Fa</creatorcontrib><creatorcontrib>Hsiao, Yi‐Hsuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Chung‐Yuan</au><au>Ho, Yung‐Chuan</au><au>Lin, Chiao‐Wen</au><au>Hsin, Min‐Chieh</au><au>Wang, Po‐Hui</au><au>Tang, Ya‐Cheng</au><au>Yang, Shun‐Fa</au><au>Hsiao, Yi‐Hsuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EF‐24 inhibits TPA‐induced cellular migration and MMP‐9 expression through the p38 signaling pathway in cervical cancer cells</atitle><jtitle>Environmental toxicology</jtitle><addtitle>Environ Toxicol</addtitle><date>2023-02</date><risdate>2023</risdate><volume>38</volume><issue>2</issue><spage>451</spage><epage>459</epage><pages>451-459</pages><issn>1520-4081</issn><eissn>1522-7278</eissn><abstract>Diphenyl difluoroketone (EF‐24), a synthetic curcumin analog, has enhanced bioavailability over curcumin. EF‐24 acts more powerful bioactivity for anti‐inflammatory and anti‐cancer activity. However, the effects and mechanism of EF‐24 on cervical cancer has not been fully investigated. Herein, this study evaluated the effects of EF‐24 on TPA‐induced cellular migration of cervical cancer. The results showed that EF‐24 substantially reduced the cellular migration and cellular invasion of the HeLa and SiHa cells. Moreover, gelatin zymography, western blotting analyses and real‐time PCR revealed that EF‐24 suppressed Matrix metalloproteinase‐9 (MMP‐9) activity, protein expression and mRNA levels. Mechanistically, EF‐24 inhibited the phosphorylation of the p38 signaling pathway. In conclusion, EF‐24 inhibited TPA‐induced cellular migration and cellular invasion of cervical cancer cell lines through modulating MMP‐9 expression via downregulating signaling p38 pathway and EF‐24 may have potential to serve as a chemopreventive agent of cervical cancer.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>36413041</pmid><doi>10.1002/tox.23709</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-0365-7927</orcidid></addata></record> |
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| ispartof | Environmental toxicology, 2023-02, Vol.38 (2), p.451-459 |
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| subjects | Bioavailability Biological activity Cancer Cell Line, Tumor Cell lines Cell Movement - drug effects Cells Cervical cancer Cervix Curcumin Curcumin - analogs & derivatives Curcumin - pharmacology EF‐24 Female Gelatin Gene expression Humans Inflammation invasion Matrix metalloproteinase Matrix Metalloproteinase 9 - genetics Matrix Metalloproteinase 9 - metabolism Matrix metalloproteinases Metalloproteinase migration MMP‐9 mRNA Neoplasm Invasiveness Nucleotide sequence Phosphorylation Signal Transduction Signaling Tumor cell lines Uterine Cervical Neoplasms - enzymology Uterine Cervical Neoplasms - pathology Western blotting |
| title | EF‐24 inhibits TPA‐induced cellular migration and MMP‐9 expression through the p38 signaling pathway in cervical cancer cells |
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