Effect of In Ovo Trace Element Supplementation on Immune-Related Cells of the Small Intestine of Post-hatched Broiler Chicken
Pathological conditions and harmful drugs cause many gastrointestinal diseases in broiler chicken. The current study was conducted to investigate the effect of trace elements zinc (Zn) and selenium (Se) supplementation on histomorphology, immunological role, and functional activity of goblet cells (...
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| Veröffentlicht in: | Biological trace element research 2023-08, Vol.201 (8), p.4052-4061 |
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| description | Pathological conditions and harmful drugs cause many gastrointestinal diseases in broiler chicken. The current study was conducted to investigate the effect of trace elements zinc (Zn) and selenium (Se) supplementation on histomorphology, immunological role, and functional activity of goblet cells (GCs) of the small intestine. The Alcian blue-periodic acid-Schiff (AB-PAS) was performed to assess the histomorphological changes in GCs, which revealed the regular dispersion with high electron density of GCs throughout the mucosal surface in the supplemented group. However, irregular dispersion with low electron density of GCs was present in the control group. The immunological functional role of GCs within the small intestine was examined by mucicarmine staining, immunohistochemistry, and immunofluorescence. The results showed a high mucin glycol protein secretion in the supplemented group, whereas limited mucin glycol protein secretion in the control group. Furthermore, the biological significance showed a high and low immunoreactivity of Muc2 and Muc13 in the supplemented and control groups, respectively. Immunofluorescence was used to confirm the immunosignaling of Muc2. Results revealed high immunosignaling of Muc2 at the apical part of the small intestine in the supplementation group, while low immunosignaling of Muc2 in the control group. Results suggest that trace element supplementation had significant effect on morphology and immunological role of GCs, which might be essential for immune function and health status of broiler chicken. |
| doi_str_mv | 10.1007/s12011-022-03492-0 |
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The current study was conducted to investigate the effect of trace elements zinc (Zn) and selenium (Se) supplementation on histomorphology, immunological role, and functional activity of goblet cells (GCs) of the small intestine. The Alcian blue-periodic acid-Schiff (AB-PAS) was performed to assess the histomorphological changes in GCs, which revealed the regular dispersion with high electron density of GCs throughout the mucosal surface in the supplemented group. However, irregular dispersion with low electron density of GCs was present in the control group. The immunological functional role of GCs within the small intestine was examined by mucicarmine staining, immunohistochemistry, and immunofluorescence. The results showed a high mucin glycol protein secretion in the supplemented group, whereas limited mucin glycol protein secretion in the control group. Furthermore, the biological significance showed a high and low immunoreactivity of Muc2 and Muc13 in the supplemented and control groups, respectively. Immunofluorescence was used to confirm the immunosignaling of Muc2. Results revealed high immunosignaling of Muc2 at the apical part of the small intestine in the supplementation group, while low immunosignaling of Muc2 in the control group. Results suggest that trace element supplementation had significant effect on morphology and immunological role of GCs, which might be essential for immune function and health status of broiler chicken.</description><identifier>ISSN: 0163-4984</identifier><identifier>EISSN: 1559-0720</identifier><identifier>DOI: 10.1007/s12011-022-03492-0</identifier><identifier>PMID: 36402885</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Chickens ; Dispersion ; Electron density ; Gastrointestinal diseases ; Goblet cells ; Immune response ; Immunofluorescence ; Immunohistochemistry ; Immunology ; Immunosuppressive agents ; Intestine ; Intestines ; Life Sciences ; Mucin ; Nutrition ; Oncology ; Poultry ; Proteins ; Secretion ; Selenium ; Small intestine ; Supplements ; Trace elements ; Zinc</subject><ispartof>Biological trace element research, 2023-08, Vol.201 (8), p.4052-4061</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-ab0e478896db4ca6e401f870d215dafbcf9f4c1261dd2b32661a39bb0ec980d83</citedby><cites>FETCH-LOGICAL-c375t-ab0e478896db4ca6e401f870d215dafbcf9f4c1261dd2b32661a39bb0ec980d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12011-022-03492-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12011-022-03492-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36402885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nabi, Fazul</creatorcontrib><creatorcontrib>Arain, Muhammad Asif</creatorcontrib><creatorcontrib>Fazlani, Sarfaraz Ali</creatorcontrib><creatorcontrib>Khalid, Mariyam</creatorcontrib><creatorcontrib>Bugti, Firdous</creatorcontrib><creatorcontrib>Ali, Sikandar</creatorcontrib><creatorcontrib>Fareed, Syed Khurram</creatorcontrib><creatorcontrib>Liu, Juan</creatorcontrib><title>Effect of In Ovo Trace Element Supplementation on Immune-Related Cells of the Small Intestine of Post-hatched Broiler Chicken</title><title>Biological trace element research</title><addtitle>Biol Trace Elem Res</addtitle><addtitle>Biol Trace Elem Res</addtitle><description>Pathological conditions and harmful drugs cause many gastrointestinal diseases in broiler chicken. The current study was conducted to investigate the effect of trace elements zinc (Zn) and selenium (Se) supplementation on histomorphology, immunological role, and functional activity of goblet cells (GCs) of the small intestine. The Alcian blue-periodic acid-Schiff (AB-PAS) was performed to assess the histomorphological changes in GCs, which revealed the regular dispersion with high electron density of GCs throughout the mucosal surface in the supplemented group. However, irregular dispersion with low electron density of GCs was present in the control group. The immunological functional role of GCs within the small intestine was examined by mucicarmine staining, immunohistochemistry, and immunofluorescence. The results showed a high mucin glycol protein secretion in the supplemented group, whereas limited mucin glycol protein secretion in the control group. Furthermore, the biological significance showed a high and low immunoreactivity of Muc2 and Muc13 in the supplemented and control groups, respectively. Immunofluorescence was used to confirm the immunosignaling of Muc2. Results revealed high immunosignaling of Muc2 at the apical part of the small intestine in the supplementation group, while low immunosignaling of Muc2 in the control group. Results suggest that trace element supplementation had significant effect on morphology and immunological role of GCs, which might be essential for immune function and health status of broiler chicken.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Chickens</subject><subject>Dispersion</subject><subject>Electron density</subject><subject>Gastrointestinal diseases</subject><subject>Goblet cells</subject><subject>Immune response</subject><subject>Immunofluorescence</subject><subject>Immunohistochemistry</subject><subject>Immunology</subject><subject>Immunosuppressive agents</subject><subject>Intestine</subject><subject>Intestines</subject><subject>Life Sciences</subject><subject>Mucin</subject><subject>Nutrition</subject><subject>Oncology</subject><subject>Poultry</subject><subject>Proteins</subject><subject>Secretion</subject><subject>Selenium</subject><subject>Small 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of In Ovo Trace Element Supplementation on Immune-Related Cells of the Small Intestine of Post-hatched Broiler Chicken</title><author>Nabi, Fazul ; Arain, Muhammad Asif ; Fazlani, Sarfaraz Ali ; Khalid, Mariyam ; Bugti, Firdous ; Ali, Sikandar ; Fareed, Syed Khurram ; Liu, Juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-ab0e478896db4ca6e401f870d215dafbcf9f4c1261dd2b32661a39bb0ec980d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Chickens</topic><topic>Dispersion</topic><topic>Electron density</topic><topic>Gastrointestinal diseases</topic><topic>Goblet cells</topic><topic>Immune response</topic><topic>Immunofluorescence</topic><topic>Immunohistochemistry</topic><topic>Immunology</topic><topic>Immunosuppressive 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Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of In Ovo Trace Element Supplementation on Immune-Related Cells of the Small Intestine of Post-hatched Broiler Chicken</atitle><jtitle>Biological trace element research</jtitle><stitle>Biol Trace Elem Res</stitle><addtitle>Biol Trace Elem Res</addtitle><date>2023-08-01</date><risdate>2023</risdate><volume>201</volume><issue>8</issue><spage>4052</spage><epage>4061</epage><pages>4052-4061</pages><issn>0163-4984</issn><eissn>1559-0720</eissn><abstract>Pathological conditions and harmful drugs cause many gastrointestinal diseases in broiler chicken. The current study was conducted to investigate the effect of trace elements zinc (Zn) and selenium (Se) supplementation on histomorphology, immunological role, and functional activity of goblet cells (GCs) of the small intestine. The Alcian blue-periodic acid-Schiff (AB-PAS) was performed to assess the histomorphological changes in GCs, which revealed the regular dispersion with high electron density of GCs throughout the mucosal surface in the supplemented group. However, irregular dispersion with low electron density of GCs was present in the control group. The immunological functional role of GCs within the small intestine was examined by mucicarmine staining, immunohistochemistry, and immunofluorescence. The results showed a high mucin glycol protein secretion in the supplemented group, whereas limited mucin glycol protein secretion in the control group. Furthermore, the biological significance showed a high and low immunoreactivity of Muc2 and Muc13 in the supplemented and control groups, respectively. Immunofluorescence was used to confirm the immunosignaling of Muc2. Results revealed high immunosignaling of Muc2 at the apical part of the small intestine in the supplementation group, while low immunosignaling of Muc2 in the control group. Results suggest that trace element supplementation had significant effect on morphology and immunological role of GCs, which might be essential for immune function and health status of broiler chicken.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>36402885</pmid><doi>10.1007/s12011-022-03492-0</doi><tpages>10</tpages></addata></record> |
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| subjects | Biochemistry Biomedical and Life Sciences Biotechnology Chickens Dispersion Electron density Gastrointestinal diseases Goblet cells Immune response Immunofluorescence Immunohistochemistry Immunology Immunosuppressive agents Intestine Intestines Life Sciences Mucin Nutrition Oncology Poultry Proteins Secretion Selenium Small intestine Supplements Trace elements Zinc |
| title | Effect of In Ovo Trace Element Supplementation on Immune-Related Cells of the Small Intestine of Post-hatched Broiler Chicken |
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