Overview and considerations in bottom-up proteomics
Proteins are the key biological actors within cells, driving many biological processes integral to both healthy and diseased states. Understanding the depth of complexity represented within the proteome is crucial to our scientific understanding of cellular biology and to provide disease specific in...
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| Veröffentlicht in: | Analyst (London) 2023-01, Vol.148 (3), p.475-486 |
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| creator | Miller, Rachel M Smith, Lloyd M |
| description | Proteins are the key biological actors within cells, driving many biological processes integral to both healthy and diseased states. Understanding the depth of complexity represented within the proteome is crucial to our scientific understanding of cellular biology and to provide disease specific insights for clinical applications. Mass spectrometry-based proteomics is the premier method for proteome analysis, with the ability to both identify and quantify proteins. Although proteomics continues to grow as a robust field of bioanalytical chemistry, advances are still necessary to enable a more comprehensive view of the proteome. In this review, we provide a broad overview of mass spectrometry-based proteomics in general, and highlight four developing areas of bottom-up proteomics: (1) protein inference, (2) alternative proteases, (3) sample-specific databases and (4) post-translational modification discovery.
This review provides a broad overview of mass spectrometry-based proteomics and highlights four developing areas: (1) protein inference, (2) alternative proteases, (3) sample-specific databases and (4) post-translational modification discovery. |
| doi_str_mv | 10.1039/d2an01246d |
| format | Article |
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| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Biological activity Mass spectrometry Mass Spectrometry - methods Peptide Hydrolases - metabolism Protein Processing, Post-Translational Proteins Proteome - metabolism Proteomics Proteomics - methods Spectroscopy |
| title | Overview and considerations in bottom-up proteomics |
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