Insight into the regulation of the Nrf2 pathway in response to ascovirus infection in Spodoptera exigua
BACKGROUND Ascoviruses are a type of entomopathogenic microorganism with high biological pest control potential and are expected to contribute to the natural control of lepidopteran pests. However, knowledge of the molecular mechanism underlying the biocidal activity of ascovirus on its host insects...
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| Veröffentlicht in: | Pest management science 2023-03, Vol.79 (3), p.1123-1130 |
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| creator | Jin, Ruoheng Xiao, Zhengkun Nakai, Madoka Huang, Guo‐Hua |
| description | BACKGROUND
Ascoviruses are a type of entomopathogenic microorganism with high biological pest control potential and are expected to contribute to the natural control of lepidopteran pests. However, knowledge of the molecular mechanism underlying the biocidal activity of ascovirus on its host insects remains limited.
RESULTS
In this study, the relative enzyme activity of superoxide dismutase and peroxidase, as well as the expression level of Spodoptera exigua peroxidase (SePOD), were found to be significantly increased at 6 h post infection with Heliothis virescens ascovirus 3h (HvAV‐3h). H2O2 accumulation and enhanced expression of NADPH Oxidase (SeNOX) were also observed. In addition, Nuclear Factor erythroid 2‐Related Factor 2 (SeNrf2) and muscle aponeurosis fibromatosis (SeMaf) were overexpressed following infection with HvAV‐3h. Silencing of SeNrf2 decreased the expression of SePOD, whereas the mortality of SeNrf2‐silenced larvae and viral genome copy number also increased. Further RNA interference of SeNOX significantly decreased expression of SeNrf2 and SePOD and therefore increased the mortality and viral genome copy number of the ascovirus‐infected host.
CONCLUSION
The HvAV‐3h activated Nrf2/ARE pathway of S. exigua and reactive oxygen species were found to respond to ascovirus infection by regulating alterations in antioxidant enzyme genes mediated by the host Nrf2/ARE pathway. These findings enhance our knowledge of ascovirus–host interactions and lay the foundation for the application of ascoviruses in biological pest control. © 2022 Society of Chemical Industry.
This study demonstrated an antioxidant system in hosts dealing with ascovirus infection. In response to reactive oxygen species damage caused by Heliothis virescens ascovirus 3h, Nrf2/ARE pathway‐mediated overexpression of antioxidant enzyme genes activates the antioxidative defense system for their own protection in Spodoptera exigua larvae. |
| doi_str_mv | 10.1002/ps.7284 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2734616596</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2734616596</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3454-49dd82188133c7a98431ffbfb22ced40d606f6e5196314304aff46f2a9e7b4773</originalsourceid><addsrcrecordid>eNp10FtLwzAYBuAgiptT_AdS8EJBNnNqDpcyPAyGClPwrqRtsnV0TU1a5_692cFdCF4lJM_38vECcI7gAEGIb2s_4FjQA9BFMWZ9KqU43N_FRweceD-HEEop8THoEEaopIh3wXRU-WI6a6KiamzUzHTk9LQtVVPYKrJm8_LsDI5q1cyWahVcEL62lddRmFA-s1-Fa334MDrbjAUyqW1u60Y7FenvYtqqU3BkVOn12e7sgfeH-7fhU3_88jga3o37GaExDcvmucBICERIxpUUlCBjUpNinOmcwpxBZpiOkWQEUQKpMoYyg5XUPKWckx643ubWzn622jfJovCZLktVadv6BHNCGWJxmO-Byz90bltXhe2C4ohQwXEc1NVWZc5677RJalcslFslCCbr7pPaJ-vug7zY5bXpQud791t2ADdbsCxKvfovJ3mdbOJ-ACGHjDM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2771348725</pqid></control><display><type>article</type><title>Insight into the regulation of the Nrf2 pathway in response to ascovirus infection in Spodoptera exigua</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Jin, Ruoheng ; Xiao, Zhengkun ; Nakai, Madoka ; Huang, Guo‐Hua</creator><creatorcontrib>Jin, Ruoheng ; Xiao, Zhengkun ; Nakai, Madoka ; Huang, Guo‐Hua</creatorcontrib><description>BACKGROUND
Ascoviruses are a type of entomopathogenic microorganism with high biological pest control potential and are expected to contribute to the natural control of lepidopteran pests. However, knowledge of the molecular mechanism underlying the biocidal activity of ascovirus on its host insects remains limited.
RESULTS
In this study, the relative enzyme activity of superoxide dismutase and peroxidase, as well as the expression level of Spodoptera exigua peroxidase (SePOD), were found to be significantly increased at 6 h post infection with Heliothis virescens ascovirus 3h (HvAV‐3h). H2O2 accumulation and enhanced expression of NADPH Oxidase (SeNOX) were also observed. In addition, Nuclear Factor erythroid 2‐Related Factor 2 (SeNrf2) and muscle aponeurosis fibromatosis (SeMaf) were overexpressed following infection with HvAV‐3h. Silencing of SeNrf2 decreased the expression of SePOD, whereas the mortality of SeNrf2‐silenced larvae and viral genome copy number also increased. Further RNA interference of SeNOX significantly decreased expression of SeNrf2 and SePOD and therefore increased the mortality and viral genome copy number of the ascovirus‐infected host.
CONCLUSION
The HvAV‐3h activated Nrf2/ARE pathway of S. exigua and reactive oxygen species were found to respond to ascovirus infection by regulating alterations in antioxidant enzyme genes mediated by the host Nrf2/ARE pathway. These findings enhance our knowledge of ascovirus–host interactions and lay the foundation for the application of ascoviruses in biological pest control. © 2022 Society of Chemical Industry.
This study demonstrated an antioxidant system in hosts dealing with ascovirus infection. In response to reactive oxygen species damage caused by Heliothis virescens ascovirus 3h, Nrf2/ARE pathway‐mediated overexpression of antioxidant enzyme genes activates the antioxidative defense system for their own protection in Spodoptera exigua larvae.</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.7284</identifier><identifier>PMID: 36349417</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; antioxidant ; Ascoviridae - genetics ; ascovirus ; Biocides ; Copy number ; Enzymatic activity ; Enzyme activity ; Enzymes ; Gene expression ; Genomes ; Hydrogen peroxide ; Infections ; Insects ; Larva - genetics ; Larvae ; Mortality ; Muscles ; NAD(P)H oxidase ; NADPH oxidase ; NF-E2-Related Factor 2 - genetics ; Nrf2 pathway ; Peroxidase ; Peroxidases ; Pest control ; Pests ; Reactive oxygen species ; RNA-mediated interference ; Spodoptera ; Spodoptera exigua ; Superoxide dismutase</subject><ispartof>Pest management science, 2023-03, Vol.79 (3), p.1123-1130</ispartof><rights>2022 Society of Chemical Industry.</rights><rights>Copyright © 2023 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3454-49dd82188133c7a98431ffbfb22ced40d606f6e5196314304aff46f2a9e7b4773</citedby><cites>FETCH-LOGICAL-c3454-49dd82188133c7a98431ffbfb22ced40d606f6e5196314304aff46f2a9e7b4773</cites><orcidid>0000-0002-6841-0095</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.7284$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.7284$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36349417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jin, Ruoheng</creatorcontrib><creatorcontrib>Xiao, Zhengkun</creatorcontrib><creatorcontrib>Nakai, Madoka</creatorcontrib><creatorcontrib>Huang, Guo‐Hua</creatorcontrib><title>Insight into the regulation of the Nrf2 pathway in response to ascovirus infection in Spodoptera exigua</title><title>Pest management science</title><addtitle>Pest Manag Sci</addtitle><description>BACKGROUND
Ascoviruses are a type of entomopathogenic microorganism with high biological pest control potential and are expected to contribute to the natural control of lepidopteran pests. However, knowledge of the molecular mechanism underlying the biocidal activity of ascovirus on its host insects remains limited.
RESULTS
In this study, the relative enzyme activity of superoxide dismutase and peroxidase, as well as the expression level of Spodoptera exigua peroxidase (SePOD), were found to be significantly increased at 6 h post infection with Heliothis virescens ascovirus 3h (HvAV‐3h). H2O2 accumulation and enhanced expression of NADPH Oxidase (SeNOX) were also observed. In addition, Nuclear Factor erythroid 2‐Related Factor 2 (SeNrf2) and muscle aponeurosis fibromatosis (SeMaf) were overexpressed following infection with HvAV‐3h. Silencing of SeNrf2 decreased the expression of SePOD, whereas the mortality of SeNrf2‐silenced larvae and viral genome copy number also increased. Further RNA interference of SeNOX significantly decreased expression of SeNrf2 and SePOD and therefore increased the mortality and viral genome copy number of the ascovirus‐infected host.
CONCLUSION
The HvAV‐3h activated Nrf2/ARE pathway of S. exigua and reactive oxygen species were found to respond to ascovirus infection by regulating alterations in antioxidant enzyme genes mediated by the host Nrf2/ARE pathway. These findings enhance our knowledge of ascovirus–host interactions and lay the foundation for the application of ascoviruses in biological pest control. © 2022 Society of Chemical Industry.
This study demonstrated an antioxidant system in hosts dealing with ascovirus infection. In response to reactive oxygen species damage caused by Heliothis virescens ascovirus 3h, Nrf2/ARE pathway‐mediated overexpression of antioxidant enzyme genes activates the antioxidative defense system for their own protection in Spodoptera exigua larvae.</description><subject>Animals</subject><subject>antioxidant</subject><subject>Ascoviridae - genetics</subject><subject>ascovirus</subject><subject>Biocides</subject><subject>Copy number</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Genomes</subject><subject>Hydrogen peroxide</subject><subject>Infections</subject><subject>Insects</subject><subject>Larva - genetics</subject><subject>Larvae</subject><subject>Mortality</subject><subject>Muscles</subject><subject>NAD(P)H oxidase</subject><subject>NADPH oxidase</subject><subject>NF-E2-Related Factor 2 - genetics</subject><subject>Nrf2 pathway</subject><subject>Peroxidase</subject><subject>Peroxidases</subject><subject>Pest control</subject><subject>Pests</subject><subject>Reactive oxygen species</subject><subject>RNA-mediated interference</subject><subject>Spodoptera</subject><subject>Spodoptera exigua</subject><subject>Superoxide dismutase</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10FtLwzAYBuAgiptT_AdS8EJBNnNqDpcyPAyGClPwrqRtsnV0TU1a5_692cFdCF4lJM_38vECcI7gAEGIb2s_4FjQA9BFMWZ9KqU43N_FRweceD-HEEop8THoEEaopIh3wXRU-WI6a6KiamzUzHTk9LQtVVPYKrJm8_LsDI5q1cyWahVcEL62lddRmFA-s1-Fa334MDrbjAUyqW1u60Y7FenvYtqqU3BkVOn12e7sgfeH-7fhU3_88jga3o37GaExDcvmucBICERIxpUUlCBjUpNinOmcwpxBZpiOkWQEUQKpMoYyg5XUPKWckx643ubWzn622jfJovCZLktVadv6BHNCGWJxmO-Byz90bltXhe2C4ohQwXEc1NVWZc5677RJalcslFslCCbr7pPaJ-vug7zY5bXpQud791t2ADdbsCxKvfovJ3mdbOJ-ACGHjDM</recordid><startdate>202303</startdate><enddate>202303</enddate><creator>Jin, Ruoheng</creator><creator>Xiao, Zhengkun</creator><creator>Nakai, Madoka</creator><creator>Huang, Guo‐Hua</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6841-0095</orcidid></search><sort><creationdate>202303</creationdate><title>Insight into the regulation of the Nrf2 pathway in response to ascovirus infection in Spodoptera exigua</title><author>Jin, Ruoheng ; Xiao, Zhengkun ; Nakai, Madoka ; Huang, Guo‐Hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3454-49dd82188133c7a98431ffbfb22ced40d606f6e5196314304aff46f2a9e7b4773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>antioxidant</topic><topic>Ascoviridae - genetics</topic><topic>ascovirus</topic><topic>Biocides</topic><topic>Copy number</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Genomes</topic><topic>Hydrogen peroxide</topic><topic>Infections</topic><topic>Insects</topic><topic>Larva - genetics</topic><topic>Larvae</topic><topic>Mortality</topic><topic>Muscles</topic><topic>NAD(P)H oxidase</topic><topic>NADPH oxidase</topic><topic>NF-E2-Related Factor 2 - genetics</topic><topic>Nrf2 pathway</topic><topic>Peroxidase</topic><topic>Peroxidases</topic><topic>Pest control</topic><topic>Pests</topic><topic>Reactive oxygen species</topic><topic>RNA-mediated interference</topic><topic>Spodoptera</topic><topic>Spodoptera exigua</topic><topic>Superoxide dismutase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jin, Ruoheng</creatorcontrib><creatorcontrib>Xiao, Zhengkun</creatorcontrib><creatorcontrib>Nakai, Madoka</creatorcontrib><creatorcontrib>Huang, Guo‐Hua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jin, Ruoheng</au><au>Xiao, Zhengkun</au><au>Nakai, Madoka</au><au>Huang, Guo‐Hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insight into the regulation of the Nrf2 pathway in response to ascovirus infection in Spodoptera exigua</atitle><jtitle>Pest management science</jtitle><addtitle>Pest Manag Sci</addtitle><date>2023-03</date><risdate>2023</risdate><volume>79</volume><issue>3</issue><spage>1123</spage><epage>1130</epage><pages>1123-1130</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>BACKGROUND
Ascoviruses are a type of entomopathogenic microorganism with high biological pest control potential and are expected to contribute to the natural control of lepidopteran pests. However, knowledge of the molecular mechanism underlying the biocidal activity of ascovirus on its host insects remains limited.
RESULTS
In this study, the relative enzyme activity of superoxide dismutase and peroxidase, as well as the expression level of Spodoptera exigua peroxidase (SePOD), were found to be significantly increased at 6 h post infection with Heliothis virescens ascovirus 3h (HvAV‐3h). H2O2 accumulation and enhanced expression of NADPH Oxidase (SeNOX) were also observed. In addition, Nuclear Factor erythroid 2‐Related Factor 2 (SeNrf2) and muscle aponeurosis fibromatosis (SeMaf) were overexpressed following infection with HvAV‐3h. Silencing of SeNrf2 decreased the expression of SePOD, whereas the mortality of SeNrf2‐silenced larvae and viral genome copy number also increased. Further RNA interference of SeNOX significantly decreased expression of SeNrf2 and SePOD and therefore increased the mortality and viral genome copy number of the ascovirus‐infected host.
CONCLUSION
The HvAV‐3h activated Nrf2/ARE pathway of S. exigua and reactive oxygen species were found to respond to ascovirus infection by regulating alterations in antioxidant enzyme genes mediated by the host Nrf2/ARE pathway. These findings enhance our knowledge of ascovirus–host interactions and lay the foundation for the application of ascoviruses in biological pest control. © 2022 Society of Chemical Industry.
This study demonstrated an antioxidant system in hosts dealing with ascovirus infection. In response to reactive oxygen species damage caused by Heliothis virescens ascovirus 3h, Nrf2/ARE pathway‐mediated overexpression of antioxidant enzyme genes activates the antioxidative defense system for their own protection in Spodoptera exigua larvae.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>36349417</pmid><doi>10.1002/ps.7284</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-6841-0095</orcidid></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals antioxidant Ascoviridae - genetics ascovirus Biocides Copy number Enzymatic activity Enzyme activity Enzymes Gene expression Genomes Hydrogen peroxide Infections Insects Larva - genetics Larvae Mortality Muscles NAD(P)H oxidase NADPH oxidase NF-E2-Related Factor 2 - genetics Nrf2 pathway Peroxidase Peroxidases Pest control Pests Reactive oxygen species RNA-mediated interference Spodoptera Spodoptera exigua Superoxide dismutase |
| title | Insight into the regulation of the Nrf2 pathway in response to ascovirus infection in Spodoptera exigua |
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