Bta-miR-200b promotes endometrial epithelial cell apoptosis by targeting MYB in cattle
Embryonic mortality is considered to be one of the main reasons for reduced conception rates in the cattle industry. Insufficient endometrial receptivity is a major factor resulting in embryo implantation failure and losses. Apoptosis of endometrial epithelial cells is an important process during es...
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Veröffentlicht in: | Theriogenology 2023-01, Vol.195, p.77-84 |
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creator | Lyu, Shijie Zhai, Yaying Zhu, Xiaoting Shi, Qiaoting Chen, Fuying Zhang, Geyang Zhang, Zijing Wang, Eryao |
description | Embryonic mortality is considered to be one of the main reasons for reduced conception rates in the cattle industry. Insufficient endometrial receptivity is a major factor resulting in embryo implantation failure and losses. Apoptosis of endometrial epithelial cells is an important process during establishment of uterine receptivity and embryo implantation. The aim of this study was to explore the role of bta-miR-200b on endometrial epithelial cell apoptosis in cattle. Overexpression of bta-miR-200b upregulated the expression of proapoptotic gene BCL2 associated X, apoptosis regulator (BAX) and endometrial receptivity marker gene osteopontin (OPN) at mRNA and protein level in bovine endometrial epithelial cells. Moreover, overexpression of bta-miR-200b was able to inhibit proliferation and promote apoptosis of bovine endometrial epithelial cells by arresting the cell cycle at the G0/G1 phase. MYB Proto-Oncogene (MYB) was verified to be a target of bta-miR-200b in bovine endometrial epithelial cells using dual-luciferase reporter assay. Transfection of bta-miR-200b mimics decreased the mRNA and protein expression of MYB. Overexpression of MYB decreased the effect of bta-miR-200b on apoptosis of bovine endometrial epithelial cells. Our findings suggest that bta-miR-200b can affect the apoptosis of endometrial epithelial cells in cattle by targeting the MYB gene.
•Bta-miR-200b regulates the expression of proapoptotic gene BAX and endometrial receptivity marker gene OPN in bovine endometrial epithelial cells.•Bta-miR-200b inhibits proliferation and promote apoptosis of bovine endometrial epithelial cells by arresting the cell cycle at the G0/G1 phase.•MYB is a target gene of bta-miR-200b in bovine endometrial epithelial cells. |
doi_str_mv | 10.1016/j.theriogenology.2022.10.006 |
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•Bta-miR-200b regulates the expression of proapoptotic gene BAX and endometrial receptivity marker gene OPN in bovine endometrial epithelial cells.•Bta-miR-200b inhibits proliferation and promote apoptosis of bovine endometrial epithelial cells by arresting the cell cycle at the G0/G1 phase.•MYB is a target gene of bta-miR-200b in bovine endometrial epithelial cells.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2022.10.006</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>Apoptosis ; Bovine ; Endometrial receptivity ; miR-200b ; Proliferation</subject><ispartof>Theriogenology, 2023-01, Vol.195, p.77-84</ispartof><rights>2022 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c304t-9f3eb5dbbc5e79aa51130c0dcc3710c04a127782e8c143f5725d793e6af020bb3</cites><orcidid>0000-0003-0232-6568</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2022.10.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Lyu, Shijie</creatorcontrib><creatorcontrib>Zhai, Yaying</creatorcontrib><creatorcontrib>Zhu, Xiaoting</creatorcontrib><creatorcontrib>Shi, Qiaoting</creatorcontrib><creatorcontrib>Chen, Fuying</creatorcontrib><creatorcontrib>Zhang, Geyang</creatorcontrib><creatorcontrib>Zhang, Zijing</creatorcontrib><creatorcontrib>Wang, Eryao</creatorcontrib><title>Bta-miR-200b promotes endometrial epithelial cell apoptosis by targeting MYB in cattle</title><title>Theriogenology</title><description>Embryonic mortality is considered to be one of the main reasons for reduced conception rates in the cattle industry. Insufficient endometrial receptivity is a major factor resulting in embryo implantation failure and losses. Apoptosis of endometrial epithelial cells is an important process during establishment of uterine receptivity and embryo implantation. The aim of this study was to explore the role of bta-miR-200b on endometrial epithelial cell apoptosis in cattle. Overexpression of bta-miR-200b upregulated the expression of proapoptotic gene BCL2 associated X, apoptosis regulator (BAX) and endometrial receptivity marker gene osteopontin (OPN) at mRNA and protein level in bovine endometrial epithelial cells. Moreover, overexpression of bta-miR-200b was able to inhibit proliferation and promote apoptosis of bovine endometrial epithelial cells by arresting the cell cycle at the G0/G1 phase. MYB Proto-Oncogene (MYB) was verified to be a target of bta-miR-200b in bovine endometrial epithelial cells using dual-luciferase reporter assay. Transfection of bta-miR-200b mimics decreased the mRNA and protein expression of MYB. Overexpression of MYB decreased the effect of bta-miR-200b on apoptosis of bovine endometrial epithelial cells. Our findings suggest that bta-miR-200b can affect the apoptosis of endometrial epithelial cells in cattle by targeting the MYB gene.
•Bta-miR-200b regulates the expression of proapoptotic gene BAX and endometrial receptivity marker gene OPN in bovine endometrial epithelial cells.•Bta-miR-200b inhibits proliferation and promote apoptosis of bovine endometrial epithelial cells by arresting the cell cycle at the G0/G1 phase.•MYB is a target gene of bta-miR-200b in bovine endometrial epithelial cells.</description><subject>Apoptosis</subject><subject>Bovine</subject><subject>Endometrial receptivity</subject><subject>miR-200b</subject><subject>Proliferation</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqNUMFKxDAUDKLguvoPOXjw0vUlaZsteHEXVwVFEBU9hTR9XbO0TU2ywv69LevFm6c3MG_mzRtCzhnMGLD8cjOLn-itW2PnGrfezThwPlAzgPyATNhcFonggh2SCUAhkrxg78fkJIQNAIg8ZxPytog6ae1zwgFK2nvXuoiBYle5FqO3uqHY2-FMM0KDTUN17_rogg203NGo_Rqj7db08WNBbUeNjrHBU3JU6ybg2e-cktfVzcvyLnl4ur1fXj8kRkAak6IWWGZVWZoMZaF1xpgAA5UxQrIBpJpxKecc54alos4kzypZCMx1DRzKUkzJxd53SP61xRBVa8OYUnfotkFxKXiWwlxmw-rVftV4F4LHWvXettrvFAM11qk26m-daqxzZIc6B_lqL8fhnW-LXgVjsTNYWY8mqsrZ_xn9AK1Qh5w</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Lyu, Shijie</creator><creator>Zhai, Yaying</creator><creator>Zhu, Xiaoting</creator><creator>Shi, Qiaoting</creator><creator>Chen, Fuying</creator><creator>Zhang, Geyang</creator><creator>Zhang, Zijing</creator><creator>Wang, Eryao</creator><general>Elsevier Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0232-6568</orcidid></search><sort><creationdate>20230101</creationdate><title>Bta-miR-200b promotes endometrial epithelial cell apoptosis by targeting MYB in cattle</title><author>Lyu, Shijie ; Zhai, Yaying ; Zhu, Xiaoting ; Shi, Qiaoting ; Chen, Fuying ; Zhang, Geyang ; Zhang, Zijing ; Wang, Eryao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c304t-9f3eb5dbbc5e79aa51130c0dcc3710c04a127782e8c143f5725d793e6af020bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Apoptosis</topic><topic>Bovine</topic><topic>Endometrial receptivity</topic><topic>miR-200b</topic><topic>Proliferation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lyu, Shijie</creatorcontrib><creatorcontrib>Zhai, Yaying</creatorcontrib><creatorcontrib>Zhu, Xiaoting</creatorcontrib><creatorcontrib>Shi, Qiaoting</creatorcontrib><creatorcontrib>Chen, Fuying</creatorcontrib><creatorcontrib>Zhang, Geyang</creatorcontrib><creatorcontrib>Zhang, Zijing</creatorcontrib><creatorcontrib>Wang, Eryao</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lyu, Shijie</au><au>Zhai, Yaying</au><au>Zhu, Xiaoting</au><au>Shi, Qiaoting</au><au>Chen, Fuying</au><au>Zhang, Geyang</au><au>Zhang, Zijing</au><au>Wang, Eryao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bta-miR-200b promotes endometrial epithelial cell apoptosis by targeting MYB in cattle</atitle><jtitle>Theriogenology</jtitle><date>2023-01-01</date><risdate>2023</risdate><volume>195</volume><spage>77</spage><epage>84</epage><pages>77-84</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>Embryonic mortality is considered to be one of the main reasons for reduced conception rates in the cattle industry. Insufficient endometrial receptivity is a major factor resulting in embryo implantation failure and losses. Apoptosis of endometrial epithelial cells is an important process during establishment of uterine receptivity and embryo implantation. The aim of this study was to explore the role of bta-miR-200b on endometrial epithelial cell apoptosis in cattle. Overexpression of bta-miR-200b upregulated the expression of proapoptotic gene BCL2 associated X, apoptosis regulator (BAX) and endometrial receptivity marker gene osteopontin (OPN) at mRNA and protein level in bovine endometrial epithelial cells. Moreover, overexpression of bta-miR-200b was able to inhibit proliferation and promote apoptosis of bovine endometrial epithelial cells by arresting the cell cycle at the G0/G1 phase. MYB Proto-Oncogene (MYB) was verified to be a target of bta-miR-200b in bovine endometrial epithelial cells using dual-luciferase reporter assay. Transfection of bta-miR-200b mimics decreased the mRNA and protein expression of MYB. Overexpression of MYB decreased the effect of bta-miR-200b on apoptosis of bovine endometrial epithelial cells. Our findings suggest that bta-miR-200b can affect the apoptosis of endometrial epithelial cells in cattle by targeting the MYB gene.
•Bta-miR-200b regulates the expression of proapoptotic gene BAX and endometrial receptivity marker gene OPN in bovine endometrial epithelial cells.•Bta-miR-200b inhibits proliferation and promote apoptosis of bovine endometrial epithelial cells by arresting the cell cycle at the G0/G1 phase.•MYB is a target gene of bta-miR-200b in bovine endometrial epithelial cells.</abstract><pub>Elsevier Inc</pub><doi>10.1016/j.theriogenology.2022.10.006</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-0232-6568</orcidid></addata></record> |
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subjects | Apoptosis Bovine Endometrial receptivity miR-200b Proliferation |
title | Bta-miR-200b promotes endometrial epithelial cell apoptosis by targeting MYB in cattle |
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