Cerebrospinal Fluid Polymerase Chain Reaction in the Diagnosis of Neonatal Bacterial Meningitis: A Single-Center Experience From Vietnam
Objective To compare the performance of cerebrospinal fluid (CSF) polymerase chain reaction (PCR) with bacterial culture for the diagnosis of neonatal bacterial meningitis (NBM). Method The CSF analysis of neonate with confirmed bacterial meningitis was performed with PCR and bacterial culture, and...
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| Veröffentlicht in: | Indian pediatrics 2022-12, Vol.59 (12), p.943-945 |
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| container_title | Indian pediatrics |
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| creator | Nguyen, Thi Quynh Nga Van Nguyen, Thi Tran, Minh Dien Skinner, Alyson Narchi, Hassib |
| description | Objective
To compare the performance of cerebrospinal fluid (CSF) polymerase chain reaction (PCR) with bacterial culture for the diagnosis of neonatal bacterial meningitis (NBM).
Method
The CSF analysis of neonate with confirmed bacterial meningitis was performed with PCR and bacterial culture, and results were compared.
Result
Among 24 neonates, the pathogens Identified included
E. coli K1
, GBS,
Streptococcus pneumoniae
and
Listeria.
PCR identified 20 (83.3%) pathogens, and culture 4 (16.7%) pathogens. Prior antibiotics were administered to 20 (83.3%) neonates in whom PCR identified 17 (85%) and culture 3 (15%) pathogens.
Conclusion
CSF PCR had a higher yield of pathogens than CSF culture in confirmed neonatal bacterial meningitis with a high rate of prior antibiotic therapy. |
| doi_str_mv | 10.1007/s13312-022-2669-0 |
| format | Article |
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To compare the performance of cerebrospinal fluid (CSF) polymerase chain reaction (PCR) with bacterial culture for the diagnosis of neonatal bacterial meningitis (NBM).
Method
The CSF analysis of neonate with confirmed bacterial meningitis was performed with PCR and bacterial culture, and results were compared.
Result
Among 24 neonates, the pathogens Identified included
E. coli K1
, GBS,
Streptococcus pneumoniae
and
Listeria.
PCR identified 20 (83.3%) pathogens, and culture 4 (16.7%) pathogens. Prior antibiotics were administered to 20 (83.3%) neonates in whom PCR identified 17 (85%) and culture 3 (15%) pathogens.
Conclusion
CSF PCR had a higher yield of pathogens than CSF culture in confirmed neonatal bacterial meningitis with a high rate of prior antibiotic therapy.</description><identifier>ISSN: 0019-6061</identifier><identifier>EISSN: 0974-7559</identifier><identifier>DOI: 10.1007/s13312-022-2669-0</identifier><identifier>PMID: 36333894</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Anti-Bacterial Agents - therapeutic use ; Bacteria ; Escherichia coli ; Humans ; Infant, Newborn ; Infant, Newborn, Diseases - drug therapy ; Maternal and Child Health ; Medicine ; Medicine & Public Health ; Meningitis, Bacterial - microbiology ; Pediatric Surgery ; Pediatrics ; Polymerase Chain Reaction - methods ; Research Paper ; Sensitivity and Specificity</subject><ispartof>Indian pediatrics, 2022-12, Vol.59 (12), p.943-945</ispartof><rights>Indian Academy of Pediatrics 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c226t-805a8e449a6e1d0b38a261fe841757450c1fad09313edc6283e8b9acb14560cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13312-022-2669-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13312-022-2669-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36333894$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nguyen, Thi Quynh Nga</creatorcontrib><creatorcontrib>Van Nguyen, Thi</creatorcontrib><creatorcontrib>Tran, Minh Dien</creatorcontrib><creatorcontrib>Skinner, Alyson</creatorcontrib><creatorcontrib>Narchi, Hassib</creatorcontrib><title>Cerebrospinal Fluid Polymerase Chain Reaction in the Diagnosis of Neonatal Bacterial Meningitis: A Single-Center Experience From Vietnam</title><title>Indian pediatrics</title><addtitle>Indian Pediatr</addtitle><addtitle>Indian Pediatr</addtitle><description>Objective
To compare the performance of cerebrospinal fluid (CSF) polymerase chain reaction (PCR) with bacterial culture for the diagnosis of neonatal bacterial meningitis (NBM).
Method
The CSF analysis of neonate with confirmed bacterial meningitis was performed with PCR and bacterial culture, and results were compared.
Result
Among 24 neonates, the pathogens Identified included
E. coli K1
, GBS,
Streptococcus pneumoniae
and
Listeria.
PCR identified 20 (83.3%) pathogens, and culture 4 (16.7%) pathogens. Prior antibiotics were administered to 20 (83.3%) neonates in whom PCR identified 17 (85%) and culture 3 (15%) pathogens.
Conclusion
CSF PCR had a higher yield of pathogens than CSF culture in confirmed neonatal bacterial meningitis with a high rate of prior antibiotic therapy.</description><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Bacteria</subject><subject>Escherichia coli</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Infant, Newborn, Diseases - drug therapy</subject><subject>Maternal and Child Health</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Meningitis, Bacterial - microbiology</subject><subject>Pediatric Surgery</subject><subject>Pediatrics</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Research Paper</subject><subject>Sensitivity and Specificity</subject><issn>0019-6061</issn><issn>0974-7559</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtP3TAUhK2qVXn1B3RTedmN4dhOnKQ7Gu4FJPoQfWwtxzm5GCX2xU4k-Af8bIwudNnVGel8M4sZQj5yOOYA1UniUnLBQAgmlGoYvCH70FQFq8qyeZs18IYpUHyPHKR0CyCkKPl7sieVlLJuin3y2GLELoa0dd6MdD0urqc_w_gwYTQJaXtjnKfXaOzsgqdZzzdIz5zZ-JBcomGg3zF4M2fz1wxhdFl9Q-_8xs0ufaGn9FfWI7IWfX7T1f02Q-gt0nUME_3rcPZmOiLvBjMm_PByD8mf9ep3e8GufpxftqdXzAqhZlZDaWosisYo5D10sjZC8QHrgldlVZRg-WB6aCSX2Fslaol11xjb8aJUYK08JJ93udsY7hZMs55csjiOxmNYkhZV7kgqVdQZ5TvU5n5SxEFvo5tMfNAc9PMAejeAzgPo5wE0ZM-nl_ilm7D_53htPANiB6T88huM-jYsMXef_pP6BGx7kYs</recordid><startdate>20221215</startdate><enddate>20221215</enddate><creator>Nguyen, Thi Quynh Nga</creator><creator>Van Nguyen, Thi</creator><creator>Tran, Minh Dien</creator><creator>Skinner, Alyson</creator><creator>Narchi, Hassib</creator><general>Springer India</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20221215</creationdate><title>Cerebrospinal Fluid Polymerase Chain Reaction in the Diagnosis of Neonatal Bacterial Meningitis: A Single-Center Experience From Vietnam</title><author>Nguyen, Thi Quynh Nga ; Van Nguyen, Thi ; Tran, Minh Dien ; Skinner, Alyson ; Narchi, Hassib</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c226t-805a8e449a6e1d0b38a261fe841757450c1fad09313edc6283e8b9acb14560cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Anti-Bacterial Agents - therapeutic use</topic><topic>Bacteria</topic><topic>Escherichia coli</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Infant, Newborn, Diseases - drug therapy</topic><topic>Maternal and Child Health</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Meningitis, Bacterial - microbiology</topic><topic>Pediatric Surgery</topic><topic>Pediatrics</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Research Paper</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nguyen, Thi Quynh Nga</creatorcontrib><creatorcontrib>Van Nguyen, Thi</creatorcontrib><creatorcontrib>Tran, Minh Dien</creatorcontrib><creatorcontrib>Skinner, Alyson</creatorcontrib><creatorcontrib>Narchi, Hassib</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Indian pediatrics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nguyen, Thi Quynh Nga</au><au>Van Nguyen, Thi</au><au>Tran, Minh Dien</au><au>Skinner, Alyson</au><au>Narchi, Hassib</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cerebrospinal Fluid Polymerase Chain Reaction in the Diagnosis of Neonatal Bacterial Meningitis: A Single-Center Experience From Vietnam</atitle><jtitle>Indian pediatrics</jtitle><stitle>Indian Pediatr</stitle><addtitle>Indian Pediatr</addtitle><date>2022-12-15</date><risdate>2022</risdate><volume>59</volume><issue>12</issue><spage>943</spage><epage>945</epage><pages>943-945</pages><issn>0019-6061</issn><eissn>0974-7559</eissn><abstract>Objective
To compare the performance of cerebrospinal fluid (CSF) polymerase chain reaction (PCR) with bacterial culture for the diagnosis of neonatal bacterial meningitis (NBM).
Method
The CSF analysis of neonate with confirmed bacterial meningitis was performed with PCR and bacterial culture, and results were compared.
Result
Among 24 neonates, the pathogens Identified included
E. coli K1
, GBS,
Streptococcus pneumoniae
and
Listeria.
PCR identified 20 (83.3%) pathogens, and culture 4 (16.7%) pathogens. Prior antibiotics were administered to 20 (83.3%) neonates in whom PCR identified 17 (85%) and culture 3 (15%) pathogens.
Conclusion
CSF PCR had a higher yield of pathogens than CSF culture in confirmed neonatal bacterial meningitis with a high rate of prior antibiotic therapy.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>36333894</pmid><doi>10.1007/s13312-022-2669-0</doi><tpages>3</tpages></addata></record> |
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| ispartof | Indian pediatrics, 2022-12, Vol.59 (12), p.943-945 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Anti-Bacterial Agents - therapeutic use Bacteria Escherichia coli Humans Infant, Newborn Infant, Newborn, Diseases - drug therapy Maternal and Child Health Medicine Medicine & Public Health Meningitis, Bacterial - microbiology Pediatric Surgery Pediatrics Polymerase Chain Reaction - methods Research Paper Sensitivity and Specificity |
| title | Cerebrospinal Fluid Polymerase Chain Reaction in the Diagnosis of Neonatal Bacterial Meningitis: A Single-Center Experience From Vietnam |
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