A one‐step multiplex PCR assay for the detection and differentiation of four species of Clarireedia causing dollar spot on turfgrass
BACKGROUND Dollar spot (DS) is one of the most destructive and economically important diseases of cool‐ and warm‐season turfgrasses worldwide. A total of six species causing DS disease in the genus Clarireedia have been described, and four of them have been reported to be distributed countrywide in...
Gespeichert in:
| Veröffentlicht in: | Pest management science 2023-03, Vol.79 (3), p.1069-1077 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1077 |
|---|---|
| container_issue | 3 |
| container_start_page | 1069 |
| container_title | Pest management science |
| container_volume | 79 |
| creator | Zhang, Huangwei Liu, Jun Dong, Yinglu Hu, Jian Lamour, Kurt Yang, Zhimin |
| description | BACKGROUND
Dollar spot (DS) is one of the most destructive and economically important diseases of cool‐ and warm‐season turfgrasses worldwide. A total of six species causing DS disease in the genus Clarireedia have been described, and four of them have been reported to be distributed countrywide in China. Identification of different species of Clarireedia is a prerequisite for the effective management of DS disease.
RESULTS
Here we report a novel polymerase chain reaction (PCR)‐based method for the detection and differentiation of the four species of Clarireedia associated with DS on turfgrass in China: C. jacksonii, C. paspali, C. monteithiana and C. hainanense. Species‐specific genes were identified for each species by comparative genomics analysis. Four primer pairs were designed and mixed to amplify species‐specific PCR fragments with differential sizes for the four species of Clarireedia in a single multiplex PCR assay. No PCR products were generated from the DNA templates of other common fungal pathogens associated with multiple turfgrass diseases. The multiplex PCR method developed can be used for the rapid and accurate detection and differentiation of the four species of Clarireedia from pure cultures as well as from infected turfgrass blades with DS symptoms.
CONCLUSION
The study developed a one‐step multiplex PCR assay for the detection and differentiation of four species of Clarireedia causing DS on turfgrass in China, which will have important implications for DS management in China and worldwide. © 2022 Society of Chemical Industry.
A one‐step multiplex PCR assay for the detection and differentiation of the four species of Clarireedia associated with dollar spot on turfgrass in China: C. jacksonii, C. paspali, C. monteithiana and C. hainanense with species‐specific PCR fragments under differential sizes. |
| doi_str_mv | 10.1002/ps.7276 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2732536321</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2771348655</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3456-6b466948be78c3a1ce7797a29f1a161ab0f06ab9cf44346fcbe404745c0dcc7d3</originalsourceid><addsrcrecordid>eNp10c1KJDEQB_AgK36t-AYS8KAg4yaddNI5yqCuICi6C96adLqikZ5Om6TZnZsnzz7jPokZRz0Ie0pS_PinkkJoh5IjSkjxY4hHspBiBW3QshATrlT17XNf3a6jzRgfCCFKqWINrTPBGCeEbqDnY-x7-Pf0EhMMeDZ2yQ0d_MVX02usY9RzbH3A6R5wCwlMcr7Hum9x66yFAH1y-q3mbYZjwHEA4yAuztNOBxcAWqex0WN0_R1ufZerWfmU78VpDPYu5Hu-o1Wruwjb7-sW-n168mv6c3JxeXY-Pb6YGMZLMRENF0LxqgFZGaapASmV1IWyVFNBdUMsEbpRxnLOuLCmAU645KUhrTGyZVvoYJk7BP84Qkz1zEUDuake_BjrQrKizL9T0Ez3vtCH_MA-d5eVpIxXoiyz2l8qE3yMAWw9BDfTYV5TUi9GUw-xXowmy933vLGZQfvpPmaRweES_HEdzP-XU1_dvMW9AoLwmQY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2771348655</pqid></control><display><type>article</type><title>A one‐step multiplex PCR assay for the detection and differentiation of four species of Clarireedia causing dollar spot on turfgrass</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Zhang, Huangwei ; Liu, Jun ; Dong, Yinglu ; Hu, Jian ; Lamour, Kurt ; Yang, Zhimin</creator><creatorcontrib>Zhang, Huangwei ; Liu, Jun ; Dong, Yinglu ; Hu, Jian ; Lamour, Kurt ; Yang, Zhimin</creatorcontrib><description>BACKGROUND
Dollar spot (DS) is one of the most destructive and economically important diseases of cool‐ and warm‐season turfgrasses worldwide. A total of six species causing DS disease in the genus Clarireedia have been described, and four of them have been reported to be distributed countrywide in China. Identification of different species of Clarireedia is a prerequisite for the effective management of DS disease.
RESULTS
Here we report a novel polymerase chain reaction (PCR)‐based method for the detection and differentiation of the four species of Clarireedia associated with DS on turfgrass in China: C. jacksonii, C. paspali, C. monteithiana and C. hainanense. Species‐specific genes were identified for each species by comparative genomics analysis. Four primer pairs were designed and mixed to amplify species‐specific PCR fragments with differential sizes for the four species of Clarireedia in a single multiplex PCR assay. No PCR products were generated from the DNA templates of other common fungal pathogens associated with multiple turfgrass diseases. The multiplex PCR method developed can be used for the rapid and accurate detection and differentiation of the four species of Clarireedia from pure cultures as well as from infected turfgrass blades with DS symptoms.
CONCLUSION
The study developed a one‐step multiplex PCR assay for the detection and differentiation of four species of Clarireedia causing DS on turfgrass in China, which will have important implications for DS management in China and worldwide. © 2022 Society of Chemical Industry.
A one‐step multiplex PCR assay for the detection and differentiation of the four species of Clarireedia associated with dollar spot on turfgrass in China: C. jacksonii, C. paspali, C. monteithiana and C. hainanense with species‐specific PCR fragments under differential sizes.</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.7276</identifier><identifier>PMID: 36334001</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Ascomycota - genetics ; Assaying ; China ; Clarireedia ; detection ; Differentiation ; Dollar spot ; Economic importance ; multiplex PCR ; Multiplex Polymerase Chain Reaction - methods ; Multiplexing ; Polymerase chain reaction ; Signs and symptoms ; Species ; turfgrass ; Turfgrasses</subject><ispartof>Pest management science, 2023-03, Vol.79 (3), p.1069-1077</ispartof><rights>2022 Society of Chemical Industry.</rights><rights>Copyright © 2023 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3456-6b466948be78c3a1ce7797a29f1a161ab0f06ab9cf44346fcbe404745c0dcc7d3</citedby><cites>FETCH-LOGICAL-c3456-6b466948be78c3a1ce7797a29f1a161ab0f06ab9cf44346fcbe404745c0dcc7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.7276$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.7276$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36334001$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Huangwei</creatorcontrib><creatorcontrib>Liu, Jun</creatorcontrib><creatorcontrib>Dong, Yinglu</creatorcontrib><creatorcontrib>Hu, Jian</creatorcontrib><creatorcontrib>Lamour, Kurt</creatorcontrib><creatorcontrib>Yang, Zhimin</creatorcontrib><title>A one‐step multiplex PCR assay for the detection and differentiation of four species of Clarireedia causing dollar spot on turfgrass</title><title>Pest management science</title><addtitle>Pest Manag Sci</addtitle><description>BACKGROUND
Dollar spot (DS) is one of the most destructive and economically important diseases of cool‐ and warm‐season turfgrasses worldwide. A total of six species causing DS disease in the genus Clarireedia have been described, and four of them have been reported to be distributed countrywide in China. Identification of different species of Clarireedia is a prerequisite for the effective management of DS disease.
RESULTS
Here we report a novel polymerase chain reaction (PCR)‐based method for the detection and differentiation of the four species of Clarireedia associated with DS on turfgrass in China: C. jacksonii, C. paspali, C. monteithiana and C. hainanense. Species‐specific genes were identified for each species by comparative genomics analysis. Four primer pairs were designed and mixed to amplify species‐specific PCR fragments with differential sizes for the four species of Clarireedia in a single multiplex PCR assay. No PCR products were generated from the DNA templates of other common fungal pathogens associated with multiple turfgrass diseases. The multiplex PCR method developed can be used for the rapid and accurate detection and differentiation of the four species of Clarireedia from pure cultures as well as from infected turfgrass blades with DS symptoms.
CONCLUSION
The study developed a one‐step multiplex PCR assay for the detection and differentiation of four species of Clarireedia causing DS on turfgrass in China, which will have important implications for DS management in China and worldwide. © 2022 Society of Chemical Industry.
A one‐step multiplex PCR assay for the detection and differentiation of the four species of Clarireedia associated with dollar spot on turfgrass in China: C. jacksonii, C. paspali, C. monteithiana and C. hainanense with species‐specific PCR fragments under differential sizes.</description><subject>Ascomycota - genetics</subject><subject>Assaying</subject><subject>China</subject><subject>Clarireedia</subject><subject>detection</subject><subject>Differentiation</subject><subject>Dollar spot</subject><subject>Economic importance</subject><subject>multiplex PCR</subject><subject>Multiplex Polymerase Chain Reaction - methods</subject><subject>Multiplexing</subject><subject>Polymerase chain reaction</subject><subject>Signs and symptoms</subject><subject>Species</subject><subject>turfgrass</subject><subject>Turfgrasses</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10c1KJDEQB_AgK36t-AYS8KAg4yaddNI5yqCuICi6C96adLqikZ5Om6TZnZsnzz7jPokZRz0Ie0pS_PinkkJoh5IjSkjxY4hHspBiBW3QshATrlT17XNf3a6jzRgfCCFKqWINrTPBGCeEbqDnY-x7-Pf0EhMMeDZ2yQ0d_MVX02usY9RzbH3A6R5wCwlMcr7Hum9x66yFAH1y-q3mbYZjwHEA4yAuztNOBxcAWqex0WN0_R1ufZerWfmU78VpDPYu5Hu-o1Wruwjb7-sW-n168mv6c3JxeXY-Pb6YGMZLMRENF0LxqgFZGaapASmV1IWyVFNBdUMsEbpRxnLOuLCmAU645KUhrTGyZVvoYJk7BP84Qkz1zEUDuake_BjrQrKizL9T0Ez3vtCH_MA-d5eVpIxXoiyz2l8qE3yMAWw9BDfTYV5TUi9GUw-xXowmy933vLGZQfvpPmaRweES_HEdzP-XU1_dvMW9AoLwmQY</recordid><startdate>202303</startdate><enddate>202303</enddate><creator>Zhang, Huangwei</creator><creator>Liu, Jun</creator><creator>Dong, Yinglu</creator><creator>Hu, Jian</creator><creator>Lamour, Kurt</creator><creator>Yang, Zhimin</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>202303</creationdate><title>A one‐step multiplex PCR assay for the detection and differentiation of four species of Clarireedia causing dollar spot on turfgrass</title><author>Zhang, Huangwei ; Liu, Jun ; Dong, Yinglu ; Hu, Jian ; Lamour, Kurt ; Yang, Zhimin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3456-6b466948be78c3a1ce7797a29f1a161ab0f06ab9cf44346fcbe404745c0dcc7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Ascomycota - genetics</topic><topic>Assaying</topic><topic>China</topic><topic>Clarireedia</topic><topic>detection</topic><topic>Differentiation</topic><topic>Dollar spot</topic><topic>Economic importance</topic><topic>multiplex PCR</topic><topic>Multiplex Polymerase Chain Reaction - methods</topic><topic>Multiplexing</topic><topic>Polymerase chain reaction</topic><topic>Signs and symptoms</topic><topic>Species</topic><topic>turfgrass</topic><topic>Turfgrasses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Huangwei</creatorcontrib><creatorcontrib>Liu, Jun</creatorcontrib><creatorcontrib>Dong, Yinglu</creatorcontrib><creatorcontrib>Hu, Jian</creatorcontrib><creatorcontrib>Lamour, Kurt</creatorcontrib><creatorcontrib>Yang, Zhimin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Huangwei</au><au>Liu, Jun</au><au>Dong, Yinglu</au><au>Hu, Jian</au><au>Lamour, Kurt</au><au>Yang, Zhimin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A one‐step multiplex PCR assay for the detection and differentiation of four species of Clarireedia causing dollar spot on turfgrass</atitle><jtitle>Pest management science</jtitle><addtitle>Pest Manag Sci</addtitle><date>2023-03</date><risdate>2023</risdate><volume>79</volume><issue>3</issue><spage>1069</spage><epage>1077</epage><pages>1069-1077</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>BACKGROUND
Dollar spot (DS) is one of the most destructive and economically important diseases of cool‐ and warm‐season turfgrasses worldwide. A total of six species causing DS disease in the genus Clarireedia have been described, and four of them have been reported to be distributed countrywide in China. Identification of different species of Clarireedia is a prerequisite for the effective management of DS disease.
RESULTS
Here we report a novel polymerase chain reaction (PCR)‐based method for the detection and differentiation of the four species of Clarireedia associated with DS on turfgrass in China: C. jacksonii, C. paspali, C. monteithiana and C. hainanense. Species‐specific genes were identified for each species by comparative genomics analysis. Four primer pairs were designed and mixed to amplify species‐specific PCR fragments with differential sizes for the four species of Clarireedia in a single multiplex PCR assay. No PCR products were generated from the DNA templates of other common fungal pathogens associated with multiple turfgrass diseases. The multiplex PCR method developed can be used for the rapid and accurate detection and differentiation of the four species of Clarireedia from pure cultures as well as from infected turfgrass blades with DS symptoms.
CONCLUSION
The study developed a one‐step multiplex PCR assay for the detection and differentiation of four species of Clarireedia causing DS on turfgrass in China, which will have important implications for DS management in China and worldwide. © 2022 Society of Chemical Industry.
A one‐step multiplex PCR assay for the detection and differentiation of the four species of Clarireedia associated with dollar spot on turfgrass in China: C. jacksonii, C. paspali, C. monteithiana and C. hainanense with species‐specific PCR fragments under differential sizes.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>36334001</pmid><doi>10.1002/ps.7276</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1526-498X |
| ispartof | Pest management science, 2023-03, Vol.79 (3), p.1069-1077 |
| issn | 1526-498X 1526-4998 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2732536321 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Ascomycota - genetics Assaying China Clarireedia detection Differentiation Dollar spot Economic importance multiplex PCR Multiplex Polymerase Chain Reaction - methods Multiplexing Polymerase chain reaction Signs and symptoms Species turfgrass Turfgrasses |
| title | A one‐step multiplex PCR assay for the detection and differentiation of four species of Clarireedia causing dollar spot on turfgrass |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T15%3A25%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20one%E2%80%90step%20multiplex%20PCR%20assay%20for%20the%20detection%20and%20differentiation%20of%20four%20species%20of%20Clarireedia%20causing%20dollar%20spot%20on%20turfgrass&rft.jtitle=Pest%20management%20science&rft.au=Zhang,%20Huangwei&rft.date=2023-03&rft.volume=79&rft.issue=3&rft.spage=1069&rft.epage=1077&rft.pages=1069-1077&rft.issn=1526-498X&rft.eissn=1526-4998&rft_id=info:doi/10.1002/ps.7276&rft_dat=%3Cproquest_cross%3E2771348655%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2771348655&rft_id=info:pmid/36334001&rfr_iscdi=true |