Lactobacillus rhamnosus GG supernatant promotes intestinal mucin production through regulating 5-HT4R and gut microbiota
Lactobacillus rhamnosus GG (LGG) is a well-known probiotic widely used in foods and drugs. It has been reported that LGG can improve bowel dysfunction in gastrointestinal motility disorders, such as constipation; however, the specific mechanisms remain unclear. The colonic mucus layer is mainly comp...
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description | Lactobacillus rhamnosus
GG (LGG) is a well-known probiotic widely used in foods and drugs. It has been reported that LGG can improve bowel dysfunction in gastrointestinal motility disorders, such as constipation; however, the specific mechanisms remain unclear. The colonic mucus layer is mainly composed of mucin secreted by goblet cells, which plays important roles in lubricating colonic contents and maintaining normal defecation function. It has been reported that increased mucin production is beneficial for relieving constipation symptoms. In this study, we aimed to investigate the role of LGG in regulating intestinal mucin production and the associated mechanisms. Six-week-old C57BL/6J mice were randomized into 3 groups, and were treated with De-Man Rogosa and Sharpe broth (MRS group), tegaserod maleate (tegaserod group) and LGG supernatant (LGGs group) by gavage, respectively. After treatments, defecation parameters, intestinal mucin-2 (MUC2) and serotonin 4 receptor (5-HT4R), goblet cells, and microbiota composition of the mice in each group were assessed. In comparison with the MRS group, higher fecal water content and increased fecal pellet number were found in the tegaserod group and LGGs group. Moreover, LGGs increased the number of goblet cells and upregulated the expression of 5-HT4R and MUC2 in the mouse colon. In addition, Alcian Blue Periodic acid Schiff staining showed that activated 5-HT4R enhanced intestinal MUC2 secretion. Further exploration of the mechanism discovered that LGGs upregulated intestinal S100A10, which was found to be involved in regulating 5-HT4R expression. Furthermore, gut microbiota analysis showed the higher abundance of
Alistipes
,
Allobaculum
,
Desulfovibrio
, and
Clostridium
XlVa in the LGGs group, which have been reported to be involved in regulating gut motility and the intestinal barrier, and alleviating bowel dysfunction. Interestingly, gut dysbiosis was present in the tegaserod group. It is noteworthy that the fecal microbiota transplanted from LGGs-treated mice significantly improved the gut dysmotility in a constipation mouse model. Our results suggested that LGGs could upregulate 5-HT4R to promote MUC2 production, as well as modulate the gut microbiota, thus improving the defecation function in mice. This finding might provide evidence for the application of diet supplementary LGG in relieving gastrointestinal motility disorders. |
doi_str_mv | 10.1039/d2fo01900k |
format | Article |
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GG (LGG) is a well-known probiotic widely used in foods and drugs. It has been reported that LGG can improve bowel dysfunction in gastrointestinal motility disorders, such as constipation; however, the specific mechanisms remain unclear. The colonic mucus layer is mainly composed of mucin secreted by goblet cells, which plays important roles in lubricating colonic contents and maintaining normal defecation function. It has been reported that increased mucin production is beneficial for relieving constipation symptoms. In this study, we aimed to investigate the role of LGG in regulating intestinal mucin production and the associated mechanisms. Six-week-old C57BL/6J mice were randomized into 3 groups, and were treated with De-Man Rogosa and Sharpe broth (MRS group), tegaserod maleate (tegaserod group) and LGG supernatant (LGGs group) by gavage, respectively. After treatments, defecation parameters, intestinal mucin-2 (MUC2) and serotonin 4 receptor (5-HT4R), goblet cells, and microbiota composition of the mice in each group were assessed. In comparison with the MRS group, higher fecal water content and increased fecal pellet number were found in the tegaserod group and LGGs group. Moreover, LGGs increased the number of goblet cells and upregulated the expression of 5-HT4R and MUC2 in the mouse colon. In addition, Alcian Blue Periodic acid Schiff staining showed that activated 5-HT4R enhanced intestinal MUC2 secretion. Further exploration of the mechanism discovered that LGGs upregulated intestinal S100A10, which was found to be involved in regulating 5-HT4R expression. Furthermore, gut microbiota analysis showed the higher abundance of
Alistipes
,
Allobaculum
,
Desulfovibrio
, and
Clostridium
XlVa in the LGGs group, which have been reported to be involved in regulating gut motility and the intestinal barrier, and alleviating bowel dysfunction. Interestingly, gut dysbiosis was present in the tegaserod group. It is noteworthy that the fecal microbiota transplanted from LGGs-treated mice significantly improved the gut dysmotility in a constipation mouse model. Our results suggested that LGGs could upregulate 5-HT4R to promote MUC2 production, as well as modulate the gut microbiota, thus improving the defecation function in mice. This finding might provide evidence for the application of diet supplementary LGG in relieving gastrointestinal motility disorders.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/d2fo01900k</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Bacteria ; Calcium-binding protein ; Constipation ; Defecation ; Digestive system ; Disorders ; Dysbacteriosis ; Fecal microflora ; Feces ; Gastric motility ; Gastrointestinal tract ; Goblet cells ; Intestinal microflora ; Intestine ; Lactobacilli ; Lactobacillus rhamnosus ; Microbiota ; Moisture content ; Motility ; Mucin ; Mucus ; Probiotics ; S100 protein ; Serotonin ; Serotonin S4 receptors ; Signs and symptoms ; Water content</subject><ispartof>Food & function, 2022-11, Vol.13 (23), p.12144-12155</ispartof><rights>Copyright Royal Society of Chemistry 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c263t-17aa9c689135e56df5441471c13c42814aa24f473f93d8d30c5dc920aae0c4b73</citedby><cites>FETCH-LOGICAL-c263t-17aa9c689135e56df5441471c13c42814aa24f473f93d8d30c5dc920aae0c4b73</cites><orcidid>0000-0002-0147-7826</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Gu, Yu</creatorcontrib><creatorcontrib>Qin, Xiali</creatorcontrib><creatorcontrib>Zhou, Guoqiong</creatorcontrib><creatorcontrib>Wang, Chen</creatorcontrib><creatorcontrib>Mu, Chenlu</creatorcontrib><creatorcontrib>Liu, Xiang</creatorcontrib><creatorcontrib>Zhong, Weilong</creatorcontrib><creatorcontrib>Xu, Xin</creatorcontrib><creatorcontrib>Wang, Bangmao</creatorcontrib><creatorcontrib>Jiang, Kui</creatorcontrib><creatorcontrib>Liu, Jinghua</creatorcontrib><creatorcontrib>Cao, Hailong</creatorcontrib><title>Lactobacillus rhamnosus GG supernatant promotes intestinal mucin production through regulating 5-HT4R and gut microbiota</title><title>Food & function</title><description>Lactobacillus rhamnosus
GG (LGG) is a well-known probiotic widely used in foods and drugs. It has been reported that LGG can improve bowel dysfunction in gastrointestinal motility disorders, such as constipation; however, the specific mechanisms remain unclear. The colonic mucus layer is mainly composed of mucin secreted by goblet cells, which plays important roles in lubricating colonic contents and maintaining normal defecation function. It has been reported that increased mucin production is beneficial for relieving constipation symptoms. In this study, we aimed to investigate the role of LGG in regulating intestinal mucin production and the associated mechanisms. Six-week-old C57BL/6J mice were randomized into 3 groups, and were treated with De-Man Rogosa and Sharpe broth (MRS group), tegaserod maleate (tegaserod group) and LGG supernatant (LGGs group) by gavage, respectively. After treatments, defecation parameters, intestinal mucin-2 (MUC2) and serotonin 4 receptor (5-HT4R), goblet cells, and microbiota composition of the mice in each group were assessed. In comparison with the MRS group, higher fecal water content and increased fecal pellet number were found in the tegaserod group and LGGs group. Moreover, LGGs increased the number of goblet cells and upregulated the expression of 5-HT4R and MUC2 in the mouse colon. In addition, Alcian Blue Periodic acid Schiff staining showed that activated 5-HT4R enhanced intestinal MUC2 secretion. Further exploration of the mechanism discovered that LGGs upregulated intestinal S100A10, which was found to be involved in regulating 5-HT4R expression. Furthermore, gut microbiota analysis showed the higher abundance of
Alistipes
,
Allobaculum
,
Desulfovibrio
, and
Clostridium
XlVa in the LGGs group, which have been reported to be involved in regulating gut motility and the intestinal barrier, and alleviating bowel dysfunction. Interestingly, gut dysbiosis was present in the tegaserod group. It is noteworthy that the fecal microbiota transplanted from LGGs-treated mice significantly improved the gut dysmotility in a constipation mouse model. Our results suggested that LGGs could upregulate 5-HT4R to promote MUC2 production, as well as modulate the gut microbiota, thus improving the defecation function in mice. This finding might provide evidence for the application of diet supplementary LGG in relieving gastrointestinal motility disorders.</description><subject>Bacteria</subject><subject>Calcium-binding protein</subject><subject>Constipation</subject><subject>Defecation</subject><subject>Digestive system</subject><subject>Disorders</subject><subject>Dysbacteriosis</subject><subject>Fecal microflora</subject><subject>Feces</subject><subject>Gastric motility</subject><subject>Gastrointestinal tract</subject><subject>Goblet cells</subject><subject>Intestinal microflora</subject><subject>Intestine</subject><subject>Lactobacilli</subject><subject>Lactobacillus rhamnosus</subject><subject>Microbiota</subject><subject>Moisture content</subject><subject>Motility</subject><subject>Mucin</subject><subject>Mucus</subject><subject>Probiotics</subject><subject>S100 protein</subject><subject>Serotonin</subject><subject>Serotonin S4 receptors</subject><subject>Signs and symptoms</subject><subject>Water content</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpdkU1LxDAQhosouKgXf0HAiwjVfLVpjuLHKi4IouCtzKZpN9omaz5A_71ZVy_OYWZgHoZ35i2KY4LPCWbyoqO9w0Ri_L5TzCjmtKwr_Lr713NZ7xdHIbzhHEzKRjaz4nMBKrolKDOOKSC_gsm6kLv5HIW01t5CBBvR2rvJRR2QsTlHY2FEU1LGbiZdUtE4i-LKuzSskNdDGiFDA6rKu2f-hMB2aEgRTUZ5tzQuwmGx18MY9NFvPShebm-er-7KxeP8_upyUSpas1gSASBV3UjCKl3VXV9xTrggijDFaUM4AOU9F6yXrGs6hlXVKUkxgMaKLwU7KE63e7POj5Slt5MJSo8jWO1SaKlgRJBGiA168g99cyk_YNxQHPP8WdZk6mxL5UtC8Lpv195M4L9agtuND-01vX388eGBfQO1HnvG</recordid><startdate>20221128</startdate><enddate>20221128</enddate><creator>Gu, Yu</creator><creator>Qin, Xiali</creator><creator>Zhou, Guoqiong</creator><creator>Wang, Chen</creator><creator>Mu, Chenlu</creator><creator>Liu, Xiang</creator><creator>Zhong, Weilong</creator><creator>Xu, Xin</creator><creator>Wang, Bangmao</creator><creator>Jiang, Kui</creator><creator>Liu, Jinghua</creator><creator>Cao, Hailong</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0147-7826</orcidid></search><sort><creationdate>20221128</creationdate><title>Lactobacillus rhamnosus GG supernatant promotes intestinal mucin production through regulating 5-HT4R and gut microbiota</title><author>Gu, Yu ; Qin, Xiali ; Zhou, Guoqiong ; Wang, Chen ; Mu, Chenlu ; Liu, Xiang ; Zhong, Weilong ; Xu, Xin ; Wang, Bangmao ; Jiang, Kui ; Liu, Jinghua ; Cao, Hailong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c263t-17aa9c689135e56df5441471c13c42814aa24f473f93d8d30c5dc920aae0c4b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Bacteria</topic><topic>Calcium-binding protein</topic><topic>Constipation</topic><topic>Defecation</topic><topic>Digestive system</topic><topic>Disorders</topic><topic>Dysbacteriosis</topic><topic>Fecal microflora</topic><topic>Feces</topic><topic>Gastric motility</topic><topic>Gastrointestinal tract</topic><topic>Goblet cells</topic><topic>Intestinal microflora</topic><topic>Intestine</topic><topic>Lactobacilli</topic><topic>Lactobacillus rhamnosus</topic><topic>Microbiota</topic><topic>Moisture content</topic><topic>Motility</topic><topic>Mucin</topic><topic>Mucus</topic><topic>Probiotics</topic><topic>S100 protein</topic><topic>Serotonin</topic><topic>Serotonin S4 receptors</topic><topic>Signs and symptoms</topic><topic>Water content</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gu, Yu</creatorcontrib><creatorcontrib>Qin, Xiali</creatorcontrib><creatorcontrib>Zhou, Guoqiong</creatorcontrib><creatorcontrib>Wang, Chen</creatorcontrib><creatorcontrib>Mu, Chenlu</creatorcontrib><creatorcontrib>Liu, Xiang</creatorcontrib><creatorcontrib>Zhong, Weilong</creatorcontrib><creatorcontrib>Xu, Xin</creatorcontrib><creatorcontrib>Wang, Bangmao</creatorcontrib><creatorcontrib>Jiang, Kui</creatorcontrib><creatorcontrib>Liu, Jinghua</creatorcontrib><creatorcontrib>Cao, Hailong</creatorcontrib><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gu, Yu</au><au>Qin, Xiali</au><au>Zhou, Guoqiong</au><au>Wang, Chen</au><au>Mu, Chenlu</au><au>Liu, Xiang</au><au>Zhong, Weilong</au><au>Xu, Xin</au><au>Wang, Bangmao</au><au>Jiang, Kui</au><au>Liu, Jinghua</au><au>Cao, Hailong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lactobacillus rhamnosus GG supernatant promotes intestinal mucin production through regulating 5-HT4R and gut microbiota</atitle><jtitle>Food & function</jtitle><date>2022-11-28</date><risdate>2022</risdate><volume>13</volume><issue>23</issue><spage>12144</spage><epage>12155</epage><pages>12144-12155</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>Lactobacillus rhamnosus
GG (LGG) is a well-known probiotic widely used in foods and drugs. It has been reported that LGG can improve bowel dysfunction in gastrointestinal motility disorders, such as constipation; however, the specific mechanisms remain unclear. The colonic mucus layer is mainly composed of mucin secreted by goblet cells, which plays important roles in lubricating colonic contents and maintaining normal defecation function. It has been reported that increased mucin production is beneficial for relieving constipation symptoms. In this study, we aimed to investigate the role of LGG in regulating intestinal mucin production and the associated mechanisms. Six-week-old C57BL/6J mice were randomized into 3 groups, and were treated with De-Man Rogosa and Sharpe broth (MRS group), tegaserod maleate (tegaserod group) and LGG supernatant (LGGs group) by gavage, respectively. After treatments, defecation parameters, intestinal mucin-2 (MUC2) and serotonin 4 receptor (5-HT4R), goblet cells, and microbiota composition of the mice in each group were assessed. In comparison with the MRS group, higher fecal water content and increased fecal pellet number were found in the tegaserod group and LGGs group. Moreover, LGGs increased the number of goblet cells and upregulated the expression of 5-HT4R and MUC2 in the mouse colon. In addition, Alcian Blue Periodic acid Schiff staining showed that activated 5-HT4R enhanced intestinal MUC2 secretion. Further exploration of the mechanism discovered that LGGs upregulated intestinal S100A10, which was found to be involved in regulating 5-HT4R expression. Furthermore, gut microbiota analysis showed the higher abundance of
Alistipes
,
Allobaculum
,
Desulfovibrio
, and
Clostridium
XlVa in the LGGs group, which have been reported to be involved in regulating gut motility and the intestinal barrier, and alleviating bowel dysfunction. Interestingly, gut dysbiosis was present in the tegaserod group. It is noteworthy that the fecal microbiota transplanted from LGGs-treated mice significantly improved the gut dysmotility in a constipation mouse model. Our results suggested that LGGs could upregulate 5-HT4R to promote MUC2 production, as well as modulate the gut microbiota, thus improving the defecation function in mice. This finding might provide evidence for the application of diet supplementary LGG in relieving gastrointestinal motility disorders.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/d2fo01900k</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-0147-7826</orcidid></addata></record> |
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subjects | Bacteria Calcium-binding protein Constipation Defecation Digestive system Disorders Dysbacteriosis Fecal microflora Feces Gastric motility Gastrointestinal tract Goblet cells Intestinal microflora Intestine Lactobacilli Lactobacillus rhamnosus Microbiota Moisture content Motility Mucin Mucus Probiotics S100 protein Serotonin Serotonin S4 receptors Signs and symptoms Water content |
title | Lactobacillus rhamnosus GG supernatant promotes intestinal mucin production through regulating 5-HT4R and gut microbiota |
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