Microneedle Array Encapsulated with Programmed DNA Hydrogels for Rapidly Sampling and Sensitively Sensing of Specific MicroRNA in Dermal Interstitial Fluid
Author: Please verify that the changes made to improve the English still retain your original meaning.Detection of microRNA (miRNA) in dermal interstitial fluid (ISF) has emerged as clinically useful in health status monitoring. However, it remains a great challenge owing to the difficult sampling a...
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| Veröffentlicht in: | ACS nano 2022-11, Vol.16 (11), p.18366-18375 |
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| creator | Yang, Qiqi Wang, Yeyu Liu, Tengfei Wu, Chaoxiong Li, Jinze Cheng, Jiale Wei, Wei Yang, Fan Zhou, Liping Zhang, Yufan Yang, Shuangshuang Dong, Haifeng |
| description | Author: Please verify that the changes made to improve the English still retain your original meaning.Detection of microRNA (miRNA) in dermal interstitial fluid (ISF) has emerged as clinically useful in health status monitoring. However, it remains a great challenge owing to the difficult sampling and low abundance. Here, we report a DNA hydrogel microneedles (MNs) array to realize rapid enrichment and sensitive detection of miRNA in ISF. The MNs’ patch consists of methacrylate hyaluronic acid (MeHA) equipped with a smart DNA circuit hydrogels’ system (MeHA/DNA), in which an appropriate miRNA input enables triggering a cascading toehold-mediated DNA displacement reaction to catalytically cleave cross-linking points to generate amplified fluorescence (FL) for miRNA detection. The MeHA/DNA-MNs patch with high mechanical strength can extract adequate ISF in a short time (0.97 ± 0.2 mg in 5 min) in vivo because of its supreme water affinity. Additionally, the cascading toehold-mediated DNA displacement signal amplification reaction allows for sensitive detection of the low-abundant miRNAs down to 241.56 pM. The DNA hydrogels’ MNs present potential for minimally invasive personalized diagnosis and real-time health monitoring in clinical applications. |
| doi_str_mv | 10.1021/acsnano.2c06261 |
| format | Article |
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However, it remains a great challenge owing to the difficult sampling and low abundance. Here, we report a DNA hydrogel microneedles (MNs) array to realize rapid enrichment and sensitive detection of miRNA in ISF. The MNs’ patch consists of methacrylate hyaluronic acid (MeHA) equipped with a smart DNA circuit hydrogels’ system (MeHA/DNA), in which an appropriate miRNA input enables triggering a cascading toehold-mediated DNA displacement reaction to catalytically cleave cross-linking points to generate amplified fluorescence (FL) for miRNA detection. The MeHA/DNA-MNs patch with high mechanical strength can extract adequate ISF in a short time (0.97 ± 0.2 mg in 5 min) in vivo because of its supreme water affinity. Additionally, the cascading toehold-mediated DNA displacement signal amplification reaction allows for sensitive detection of the low-abundant miRNAs down to 241.56 pM. The DNA hydrogels’ MNs present potential for minimally invasive personalized diagnosis and real-time health monitoring in clinical applications.</description><identifier>ISSN: 1936-0851</identifier><identifier>EISSN: 1936-086X</identifier><identifier>DOI: 10.1021/acsnano.2c06261</identifier><identifier>PMID: 36326107</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>DNA - genetics ; Extracellular Fluid ; Hydrogels ; MicroRNAs - genetics ; Needles</subject><ispartof>ACS nano, 2022-11, Vol.16 (11), p.18366-18375</ispartof><rights>2022 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a263t-3af7e0daa178802db1a65a29fc90e8603fb9e397c6419453cb953059c6dcab793</citedby><cites>FETCH-LOGICAL-a263t-3af7e0daa178802db1a65a29fc90e8603fb9e397c6419453cb953059c6dcab793</cites><orcidid>0000-0002-6907-6578</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acsnano.2c06261$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acsnano.2c06261$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27075,27923,27924,56737,56787</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36326107$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Qiqi</creatorcontrib><creatorcontrib>Wang, Yeyu</creatorcontrib><creatorcontrib>Liu, Tengfei</creatorcontrib><creatorcontrib>Wu, Chaoxiong</creatorcontrib><creatorcontrib>Li, Jinze</creatorcontrib><creatorcontrib>Cheng, Jiale</creatorcontrib><creatorcontrib>Wei, Wei</creatorcontrib><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>Zhou, Liping</creatorcontrib><creatorcontrib>Zhang, Yufan</creatorcontrib><creatorcontrib>Yang, Shuangshuang</creatorcontrib><creatorcontrib>Dong, Haifeng</creatorcontrib><title>Microneedle Array Encapsulated with Programmed DNA Hydrogels for Rapidly Sampling and Sensitively Sensing of Specific MicroRNA in Dermal Interstitial Fluid</title><title>ACS nano</title><addtitle>ACS Nano</addtitle><description>Author: Please verify that the changes made to improve the English still retain your original meaning.Detection of microRNA (miRNA) in dermal interstitial fluid (ISF) has emerged as clinically useful in health status monitoring. However, it remains a great challenge owing to the difficult sampling and low abundance. Here, we report a DNA hydrogel microneedles (MNs) array to realize rapid enrichment and sensitive detection of miRNA in ISF. The MNs’ patch consists of methacrylate hyaluronic acid (MeHA) equipped with a smart DNA circuit hydrogels’ system (MeHA/DNA), in which an appropriate miRNA input enables triggering a cascading toehold-mediated DNA displacement reaction to catalytically cleave cross-linking points to generate amplified fluorescence (FL) for miRNA detection. The MeHA/DNA-MNs patch with high mechanical strength can extract adequate ISF in a short time (0.97 ± 0.2 mg in 5 min) in vivo because of its supreme water affinity. Additionally, the cascading toehold-mediated DNA displacement signal amplification reaction allows for sensitive detection of the low-abundant miRNAs down to 241.56 pM. The DNA hydrogels’ MNs present potential for minimally invasive personalized diagnosis and real-time health monitoring in clinical applications.</description><subject>DNA - genetics</subject><subject>Extracellular Fluid</subject><subject>Hydrogels</subject><subject>MicroRNAs - genetics</subject><subject>Needles</subject><issn>1936-0851</issn><issn>1936-086X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtv3CAUhVGUKK92nV3EMlI1CZgYzHKUt5RHlWml7qxruE6IMHbBbjW_pX-2TGeaXVbce_nO4XEIOeLslLOCn4FJAUJ_WhgmC8m3yD7XQs5YJX9sv9cl3yMHKb0xVqpKyV2yJ6TINFP75M-DM7EPiNYjnccIS3oVDAxp8jCipb_d-Eq_xv4lQtfl_vJxTm-XNg_QJ9r2kT7D4Kxf0gV0g3fhhUKwdIEhudH9wtXGqs7zvqWLAY1rnaH_Tn3OXi7QS4wdeHoXRoxpzKrcXPvJ2U9kpwWf8PNmPSTfr6--XdzO7p9u7i7m9zMopBhnAlqFzAJwVVWssA0HWUKhW6MZVpKJttEotDLynOvzUphGl4KV2khroFFaHJKTte8Q-58TprHuXDLoPQTsp1QXSnDFq1Ks0LM1mq-fUsS2HqLrIC5rzupVIvUmkXqTSFYcb8ynJn_gO_8_ggx8WQNZWb_1Uwz5rR_a_QVS15lk</recordid><startdate>20221122</startdate><enddate>20221122</enddate><creator>Yang, Qiqi</creator><creator>Wang, Yeyu</creator><creator>Liu, Tengfei</creator><creator>Wu, Chaoxiong</creator><creator>Li, Jinze</creator><creator>Cheng, Jiale</creator><creator>Wei, Wei</creator><creator>Yang, Fan</creator><creator>Zhou, Liping</creator><creator>Zhang, Yufan</creator><creator>Yang, Shuangshuang</creator><creator>Dong, Haifeng</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6907-6578</orcidid></search><sort><creationdate>20221122</creationdate><title>Microneedle Array Encapsulated with Programmed DNA Hydrogels for Rapidly Sampling and Sensitively Sensing of Specific MicroRNA in Dermal Interstitial Fluid</title><author>Yang, Qiqi ; Wang, Yeyu ; Liu, Tengfei ; Wu, Chaoxiong ; Li, Jinze ; Cheng, Jiale ; Wei, Wei ; Yang, Fan ; Zhou, Liping ; Zhang, Yufan ; Yang, Shuangshuang ; Dong, Haifeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a263t-3af7e0daa178802db1a65a29fc90e8603fb9e397c6419453cb953059c6dcab793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>DNA - genetics</topic><topic>Extracellular Fluid</topic><topic>Hydrogels</topic><topic>MicroRNAs - genetics</topic><topic>Needles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Qiqi</creatorcontrib><creatorcontrib>Wang, Yeyu</creatorcontrib><creatorcontrib>Liu, Tengfei</creatorcontrib><creatorcontrib>Wu, Chaoxiong</creatorcontrib><creatorcontrib>Li, Jinze</creatorcontrib><creatorcontrib>Cheng, Jiale</creatorcontrib><creatorcontrib>Wei, Wei</creatorcontrib><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>Zhou, Liping</creatorcontrib><creatorcontrib>Zhang, Yufan</creatorcontrib><creatorcontrib>Yang, Shuangshuang</creatorcontrib><creatorcontrib>Dong, Haifeng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Qiqi</au><au>Wang, Yeyu</au><au>Liu, Tengfei</au><au>Wu, Chaoxiong</au><au>Li, Jinze</au><au>Cheng, Jiale</au><au>Wei, Wei</au><au>Yang, Fan</au><au>Zhou, Liping</au><au>Zhang, Yufan</au><au>Yang, Shuangshuang</au><au>Dong, Haifeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microneedle Array Encapsulated with Programmed DNA Hydrogels for Rapidly Sampling and Sensitively Sensing of Specific MicroRNA in Dermal Interstitial Fluid</atitle><jtitle>ACS nano</jtitle><addtitle>ACS Nano</addtitle><date>2022-11-22</date><risdate>2022</risdate><volume>16</volume><issue>11</issue><spage>18366</spage><epage>18375</epage><pages>18366-18375</pages><issn>1936-0851</issn><eissn>1936-086X</eissn><abstract>Author: Please verify that the changes made to improve the English still retain your original meaning.Detection of microRNA (miRNA) in dermal interstitial fluid (ISF) has emerged as clinically useful in health status monitoring. However, it remains a great challenge owing to the difficult sampling and low abundance. Here, we report a DNA hydrogel microneedles (MNs) array to realize rapid enrichment and sensitive detection of miRNA in ISF. The MNs’ patch consists of methacrylate hyaluronic acid (MeHA) equipped with a smart DNA circuit hydrogels’ system (MeHA/DNA), in which an appropriate miRNA input enables triggering a cascading toehold-mediated DNA displacement reaction to catalytically cleave cross-linking points to generate amplified fluorescence (FL) for miRNA detection. The MeHA/DNA-MNs patch with high mechanical strength can extract adequate ISF in a short time (0.97 ± 0.2 mg in 5 min) in vivo because of its supreme water affinity. Additionally, the cascading toehold-mediated DNA displacement signal amplification reaction allows for sensitive detection of the low-abundant miRNAs down to 241.56 pM. 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| source | MEDLINE; ACS Publications |
| subjects | DNA - genetics Extracellular Fluid Hydrogels MicroRNAs - genetics Needles |
| title | Microneedle Array Encapsulated with Programmed DNA Hydrogels for Rapidly Sampling and Sensitively Sensing of Specific MicroRNA in Dermal Interstitial Fluid |
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