Dopamine inhibits pyroptosis and attenuates secondary damage after spinal cord injury in female mice
•Dopamine suppresses NLRP3 inflammasome activation and pyroptosis.•Dopamine attenuates neuroinflammation.•Dopamine alleviates secondary damage. An excessive inflammatory response accompanies the pathogenesis of spinal cord injury (SCI) and has been found to be promoted by inflammasomes in a variety...
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| Veröffentlicht in: | Neuroscience letters 2023-01, Vol.792, p.136935-136935, Article 136935 |
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| container_title | Neuroscience letters |
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| creator | Jiang, Wu He, Fan Ding, Guoming Wu, Junsong |
| description | •Dopamine suppresses NLRP3 inflammasome activation and pyroptosis.•Dopamine attenuates neuroinflammation.•Dopamine alleviates secondary damage.
An excessive inflammatory response accompanies the pathogenesis of spinal cord injury (SCI) and has been found to be promoted by inflammasomes in a variety of disease models. Dopamine is a neurotransmitter that also regulates nucleotide-binding oligomerization domain-like receptor 3 (NLRP3) inflammasome-dependent neuroinflammation. However, little is known regarding the effects and molecular mechanisms underlying the role of dopamine in SCI.
Functional recovery in mice was assessed with the Basso Mouse Scale (BMS). Neuronal loss was evaluated with immunochemical staining of NeuN. Pyroptosis was assessed with immunofluorescence staining, flow cytometry, western blotting, and cell viability and cytotoxicity assays.
Dopamine was significantly associated with enhanced locomotor recovery after SCI, and with decreased NLRP3 inflammasome activation, pyroptosis, neuronal loss and pro-inflammatory cytokine levels. In vitro data suggested that dopamine suppressed NLRP3 inflammasome activation and pyroptosis, and decreased pro-inflammatory cytokine levels. Conclusions: Dopamine may be a novel approach for alleviating secondary damage after SCI. |
| doi_str_mv | 10.1016/j.neulet.2022.136935 |
| format | Article |
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An excessive inflammatory response accompanies the pathogenesis of spinal cord injury (SCI) and has been found to be promoted by inflammasomes in a variety of disease models. Dopamine is a neurotransmitter that also regulates nucleotide-binding oligomerization domain-like receptor 3 (NLRP3) inflammasome-dependent neuroinflammation. However, little is known regarding the effects and molecular mechanisms underlying the role of dopamine in SCI.
Functional recovery in mice was assessed with the Basso Mouse Scale (BMS). Neuronal loss was evaluated with immunochemical staining of NeuN. Pyroptosis was assessed with immunofluorescence staining, flow cytometry, western blotting, and cell viability and cytotoxicity assays.
Dopamine was significantly associated with enhanced locomotor recovery after SCI, and with decreased NLRP3 inflammasome activation, pyroptosis, neuronal loss and pro-inflammatory cytokine levels. In vitro data suggested that dopamine suppressed NLRP3 inflammasome activation and pyroptosis, and decreased pro-inflammatory cytokine levels. Conclusions: Dopamine may be a novel approach for alleviating secondary damage after SCI.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2022.136935</identifier><identifier>PMID: 36307053</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Animals ; Cytokines - pharmacology ; Dopamine ; Dopamine - pharmacology ; Female ; Inflammasome ; Inflammasomes ; Inflammatory response ; Mice ; Neuronal loss ; NLR Family, Pyrin Domain-Containing 3 Protein ; Pyroptosis ; Pyroptosis - physiology ; Spinal Cord - pathology ; Spinal Cord Injuries - drug therapy ; Spinal Cord Injuries - pathology</subject><ispartof>Neuroscience letters, 2023-01, Vol.792, p.136935-136935, Article 136935</ispartof><rights>2022</rights><rights>Copyright © 2022. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c292t-2f800df653eab262b89958688ff3a1426768ae7c3daf917f473ed69b6233fc673</citedby><cites>FETCH-LOGICAL-c292t-2f800df653eab262b89958688ff3a1426768ae7c3daf917f473ed69b6233fc673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.neulet.2022.136935$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36307053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Wu</creatorcontrib><creatorcontrib>He, Fan</creatorcontrib><creatorcontrib>Ding, Guoming</creatorcontrib><creatorcontrib>Wu, Junsong</creatorcontrib><title>Dopamine inhibits pyroptosis and attenuates secondary damage after spinal cord injury in female mice</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>•Dopamine suppresses NLRP3 inflammasome activation and pyroptosis.•Dopamine attenuates neuroinflammation.•Dopamine alleviates secondary damage.
An excessive inflammatory response accompanies the pathogenesis of spinal cord injury (SCI) and has been found to be promoted by inflammasomes in a variety of disease models. Dopamine is a neurotransmitter that also regulates nucleotide-binding oligomerization domain-like receptor 3 (NLRP3) inflammasome-dependent neuroinflammation. However, little is known regarding the effects and molecular mechanisms underlying the role of dopamine in SCI.
Functional recovery in mice was assessed with the Basso Mouse Scale (BMS). Neuronal loss was evaluated with immunochemical staining of NeuN. Pyroptosis was assessed with immunofluorescence staining, flow cytometry, western blotting, and cell viability and cytotoxicity assays.
Dopamine was significantly associated with enhanced locomotor recovery after SCI, and with decreased NLRP3 inflammasome activation, pyroptosis, neuronal loss and pro-inflammatory cytokine levels. In vitro data suggested that dopamine suppressed NLRP3 inflammasome activation and pyroptosis, and decreased pro-inflammatory cytokine levels. Conclusions: Dopamine may be a novel approach for alleviating secondary damage after SCI.</description><subject>Animals</subject><subject>Cytokines - pharmacology</subject><subject>Dopamine</subject><subject>Dopamine - pharmacology</subject><subject>Female</subject><subject>Inflammasome</subject><subject>Inflammasomes</subject><subject>Inflammatory response</subject><subject>Mice</subject><subject>Neuronal loss</subject><subject>NLR Family, Pyrin Domain-Containing 3 Protein</subject><subject>Pyroptosis</subject><subject>Pyroptosis - physiology</subject><subject>Spinal Cord - pathology</subject><subject>Spinal Cord Injuries - drug therapy</subject><subject>Spinal Cord Injuries - pathology</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1L5TAUhoM46NXxH4hk6abXfLRJuxFEHWdAcDOzDmlyorm0SU1SwX9vL9VZujqL837wPgidU7KlhIqr3TbAPEDZMsLYlnLR8eYAbWgrWSU7yQ7RhnBSV7yryTE6yXlHCGloUx-hYy44kaThG2Tv4qRHHwD78OJ7XzKe3lOcSsw-Yx0s1qVAmHWBjDOYGKxO79jqUT8D1q5AwnnyQQ_YxGSXlN28_H3ADkY9AB69gZ_oh9NDhrPPe4r-_br_e_u7enx6-HN781gZ1rFSMdcSYp1oOOieCda3Xde0om2d45rWTEjRapCGW-06Kl0tOVjR9YJx7oyQ_BRdrrlTiq8z5KJGnw0Mgw4Q56yY5IQvBFq2SOtValLMOYFTU_LjMk1RovZ81U6tfNWer1r5LraLz4a5H8H-N30BXQTXqwCWnW8eksrGQzBgfQJTlI3--4YPnqqO4Q</recordid><startdate>20230101</startdate><enddate>20230101</enddate><creator>Jiang, Wu</creator><creator>He, Fan</creator><creator>Ding, Guoming</creator><creator>Wu, Junsong</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20230101</creationdate><title>Dopamine inhibits pyroptosis and attenuates secondary damage after spinal cord injury in female mice</title><author>Jiang, Wu ; He, Fan ; Ding, Guoming ; Wu, Junsong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c292t-2f800df653eab262b89958688ff3a1426768ae7c3daf917f473ed69b6233fc673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Cytokines - pharmacology</topic><topic>Dopamine</topic><topic>Dopamine - pharmacology</topic><topic>Female</topic><topic>Inflammasome</topic><topic>Inflammasomes</topic><topic>Inflammatory response</topic><topic>Mice</topic><topic>Neuronal loss</topic><topic>NLR Family, Pyrin Domain-Containing 3 Protein</topic><topic>Pyroptosis</topic><topic>Pyroptosis - physiology</topic><topic>Spinal Cord - pathology</topic><topic>Spinal Cord Injuries - drug therapy</topic><topic>Spinal Cord Injuries - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Wu</creatorcontrib><creatorcontrib>He, Fan</creatorcontrib><creatorcontrib>Ding, Guoming</creatorcontrib><creatorcontrib>Wu, Junsong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Wu</au><au>He, Fan</au><au>Ding, Guoming</au><au>Wu, Junsong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dopamine inhibits pyroptosis and attenuates secondary damage after spinal cord injury in female mice</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2023-01-01</date><risdate>2023</risdate><volume>792</volume><spage>136935</spage><epage>136935</epage><pages>136935-136935</pages><artnum>136935</artnum><issn>0304-3940</issn><eissn>1872-7972</eissn><abstract>•Dopamine suppresses NLRP3 inflammasome activation and pyroptosis.•Dopamine attenuates neuroinflammation.•Dopamine alleviates secondary damage.
An excessive inflammatory response accompanies the pathogenesis of spinal cord injury (SCI) and has been found to be promoted by inflammasomes in a variety of disease models. Dopamine is a neurotransmitter that also regulates nucleotide-binding oligomerization domain-like receptor 3 (NLRP3) inflammasome-dependent neuroinflammation. However, little is known regarding the effects and molecular mechanisms underlying the role of dopamine in SCI.
Functional recovery in mice was assessed with the Basso Mouse Scale (BMS). Neuronal loss was evaluated with immunochemical staining of NeuN. Pyroptosis was assessed with immunofluorescence staining, flow cytometry, western blotting, and cell viability and cytotoxicity assays.
Dopamine was significantly associated with enhanced locomotor recovery after SCI, and with decreased NLRP3 inflammasome activation, pyroptosis, neuronal loss and pro-inflammatory cytokine levels. In vitro data suggested that dopamine suppressed NLRP3 inflammasome activation and pyroptosis, and decreased pro-inflammatory cytokine levels. Conclusions: Dopamine may be a novel approach for alleviating secondary damage after SCI.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>36307053</pmid><doi>10.1016/j.neulet.2022.136935</doi><tpages>1</tpages></addata></record> |
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| ispartof | Neuroscience letters, 2023-01, Vol.792, p.136935-136935, Article 136935 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cytokines - pharmacology Dopamine Dopamine - pharmacology Female Inflammasome Inflammasomes Inflammatory response Mice Neuronal loss NLR Family, Pyrin Domain-Containing 3 Protein Pyroptosis Pyroptosis - physiology Spinal Cord - pathology Spinal Cord Injuries - drug therapy Spinal Cord Injuries - pathology |
| title | Dopamine inhibits pyroptosis and attenuates secondary damage after spinal cord injury in female mice |
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