A developmental atlas of somatosensory diversification and maturation in the dorsal root ganglia by single-cell mass cytometry
Precisely controlled development of the somatosensory system is essential for detecting pain, itch, temperature, mechanical touch and body position. To investigate the protein-level changes that occur during somatosensory development, we performed single-cell mass cytometry on dorsal root ganglia fr...
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| Veröffentlicht in: | Nature neuroscience 2022-11, Vol.25 (11), p.1543-1558 |
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| creator | Keeler, Austin B. Van Deusen, Amy L. Gadani, Irene C. Williams, Corey M. Goggin, Sarah M. Hirt, Ashley K. Vradenburgh, Shayla A. Fread, Kristen I. Puleo, Emily A. Jin, Lucy Calhan, O. Yipkin Deppmann, Christopher D. Zunder, Eli R. |
| description | Precisely controlled development of the somatosensory system is essential for detecting pain, itch, temperature, mechanical touch and body position. To investigate the protein-level changes that occur during somatosensory development, we performed single-cell mass cytometry on dorsal root ganglia from C57/BL6 mice of both sexes, with litter replicates collected daily from embryonic day 11.5 to postnatal day 4. Measuring nearly 3 million cells, we quantified 30 molecularly distinct somatosensory glial and 41 distinct neuronal states across all timepoints. Analysis of differentiation trajectories revealed rare cells that co-express two or more Trk receptors and over-express stem cell markers, suggesting that these neurotrophic factor receptors play a role in cell fate specification. Comparison to previous RNA-based studies identified substantial differences between many protein–mRNA pairs, demonstrating the importance of protein-level measurements to identify functional cell states. Overall, this study demonstrates that mass cytometry is a high-throughput, scalable platform to rapidly phenotype somatosensory tissues.
Somatosensory neurons detect pain, temperature and touch. Keeler et al. constructed a single-cell, protein-level atlas of nearly 3 million cells from the mouse dorsal root ganglia, covering 13 days of embryonic and postnatal development. |
| doi_str_mv | 10.1038/s41593-022-01181-8 |
| format | Article |
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Somatosensory neurons detect pain, temperature and touch. Keeler et al. constructed a single-cell, protein-level atlas of nearly 3 million cells from the mouse dorsal root ganglia, covering 13 days of embryonic and postnatal development.</description><subject>13/1</subject><subject>13/31</subject><subject>13/51</subject><subject>14/63</subject><subject>631/378/2571/1696</subject><subject>631/378/2571/219</subject><subject>631/378/2571/2573</subject><subject>631/378/2620</subject><subject>64/60</subject><subject>82/58</subject><subject>82/80</subject><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Behavioral Sciences</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Body temperature</subject><subject>Cell Differentiation</subject><subject>Cell fate</subject><subject>Cytometry</subject><subject>Dorsal root ganglia</subject><subject>Embryogenesis</subject><subject>Female</subject><subject>Ganglia</subject><subject>Ganglia, Spinal - physiology</subject><subject>Male</subject><subject>Maturation</subject><subject>Mice</subject><subject>mRNA</subject><subject>Neurobiology</subject><subject>Neuroglia</subject><subject>Neuronal-glial interactions</subject><subject>Neurons - physiology</subject><subject>Neurosciences</subject><subject>Neurotrophic factors</subject><subject>Neurotrophin receptors</subject><subject>Pain</subject><subject>Phenotypes</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Resource</subject><subject>RNA, Messenger - genetics</subject><subject>Somatosensory system</subject><subject>Stem cells</subject><subject>Trajectory analysis</subject><subject>Trk receptors</subject><issn>1097-6256</issn><issn>1546-1726</issn><issn>1546-1726</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUuLFTEQhRtRnIf-ARcScOMmmkfntRyG0REG3Og6pNPpa4bu5JpKD_TG326uPSq4cFUV6junipyue0XJO0q4fg89FYZjwhgmlGqK9ZPunIpeYqqYfNp6YhSWTMiz7gLgnhCihDbPuzMuOeFE6vPuxxUaw0OY83EJqboZuTo7QHlCkBdXM4QEuWxojA-hQJyidzXmhFwaUZuvZX_GhOq3gMZcoHmUnCs6uHSYo0PDhiC2NmAf5rmJAJDfal5CLduL7tnkZggvH-tl9_XDzZfrW3z3-eOn66s77HtqKma98oPgXns2eU6JdF4ZJbwWlDjPpZCmVTE4Q4nmgRHCOdNUOTIYM-iRX3Zvd99jyd_XANUuEU73uBTyCpap9iG0F4Y19M0_6H1eS2rXnSjKm7nqG8V2ypcMUMJkjyUurmyWEntKx-7p2JaO_ZWO1U30-tF6HZYw_pH8jqMBfAegjdIhlL-7_2P7EyN0m4c</recordid><startdate>20221101</startdate><enddate>20221101</enddate><creator>Keeler, Austin B.</creator><creator>Van Deusen, Amy L.</creator><creator>Gadani, Irene C.</creator><creator>Williams, Corey M.</creator><creator>Goggin, Sarah M.</creator><creator>Hirt, Ashley K.</creator><creator>Vradenburgh, Shayla A.</creator><creator>Fread, Kristen I.</creator><creator>Puleo, Emily A.</creator><creator>Jin, Lucy</creator><creator>Calhan, O. 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Yipkin</au><au>Deppmann, Christopher D.</au><au>Zunder, Eli R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A developmental atlas of somatosensory diversification and maturation in the dorsal root ganglia by single-cell mass cytometry</atitle><jtitle>Nature neuroscience</jtitle><stitle>Nat Neurosci</stitle><addtitle>Nat Neurosci</addtitle><date>2022-11-01</date><risdate>2022</risdate><volume>25</volume><issue>11</issue><spage>1543</spage><epage>1558</epage><pages>1543-1558</pages><issn>1097-6256</issn><issn>1546-1726</issn><eissn>1546-1726</eissn><abstract>Precisely controlled development of the somatosensory system is essential for detecting pain, itch, temperature, mechanical touch and body position. To investigate the protein-level changes that occur during somatosensory development, we performed single-cell mass cytometry on dorsal root ganglia from C57/BL6 mice of both sexes, with litter replicates collected daily from embryonic day 11.5 to postnatal day 4. Measuring nearly 3 million cells, we quantified 30 molecularly distinct somatosensory glial and 41 distinct neuronal states across all timepoints. Analysis of differentiation trajectories revealed rare cells that co-express two or more Trk receptors and over-express stem cell markers, suggesting that these neurotrophic factor receptors play a role in cell fate specification. Comparison to previous RNA-based studies identified substantial differences between many protein–mRNA pairs, demonstrating the importance of protein-level measurements to identify functional cell states. Overall, this study demonstrates that mass cytometry is a high-throughput, scalable platform to rapidly phenotype somatosensory tissues.
Somatosensory neurons detect pain, temperature and touch. Keeler et al. constructed a single-cell, protein-level atlas of nearly 3 million cells from the mouse dorsal root ganglia, covering 13 days of embryonic and postnatal development.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>36303068</pmid><doi>10.1038/s41593-022-01181-8</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0001-5630-6533</orcidid><orcidid>https://orcid.org/0000-0002-6591-1767</orcidid><orcidid>https://orcid.org/0000-0002-0356-1685</orcidid><orcidid>https://orcid.org/0000-0002-6427-0283</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 13/1 13/31 13/51 14/63 631/378/2571/1696 631/378/2571/219 631/378/2571/2573 631/378/2620 64/60 82/58 82/80 Animal Genetics and Genomics Animals Behavioral Sciences Biological Techniques Biomedical and Life Sciences Biomedicine Body temperature Cell Differentiation Cell fate Cytometry Dorsal root ganglia Embryogenesis Female Ganglia Ganglia, Spinal - physiology Male Maturation Mice mRNA Neurobiology Neuroglia Neuronal-glial interactions Neurons - physiology Neurosciences Neurotrophic factors Neurotrophin receptors Pain Phenotypes Proteins Receptors Resource RNA, Messenger - genetics Somatosensory system Stem cells Trajectory analysis Trk receptors |
| title | A developmental atlas of somatosensory diversification and maturation in the dorsal root ganglia by single-cell mass cytometry |
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