Adenylate cyclase activity of TIR1/AFB auxin receptors in plants
The phytohormone auxin is the major coordinative signal in plant development 1 , mediating transcriptional reprogramming by a well-established canonical signalling pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin receptors are F-box subunits of ubiquitin ligase comple...
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| Veröffentlicht in: | Nature (London) 2022-11, Vol.611 (7934), p.133-138 |
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| creator | Qi, Linlin Kwiatkowski, Mateusz Chen, Huihuang Hoermayer, Lukas Sinclair, Scott Zou, Minxia del Genio, Charo I. Kubeš, Martin F. Napier, Richard Jaworski, Krzysztof Friml, Jiří |
| description | The phytohormone auxin is the major coordinative signal in plant development
1
, mediating transcriptional reprogramming by a well-established canonical signalling pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin, they associate with Aux/IAA transcriptional repressors and target them for degradation via ubiquitination
2
,
3
. Here we identify adenylate cyclase (AC) activity as an additional function of TIR1/AFB receptors across land plants. Auxin, together with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC motif of the TIR1 C-terminal region, all of which abolish the AC activity, each render TIR1 ineffective in mediating gravitropism and sustained auxin-induced root growth inhibition, and also affect auxin-induced transcriptional regulation. These results highlight the importance of TIR1/AFB AC activity in canonical auxin signalling. They also identify a unique phytohormone receptor cassette combining F-box and AC motifs, and the role of cAMP as a second messenger in plants.
Adenylate cyclase activity in TIR1/AFB, the canonical auxin receptor, has an essential role in auxin-mediated root growth inhibition in land plants. |
| doi_str_mv | 10.1038/s41586-022-05369-7 |
| format | Article |
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1
, mediating transcriptional reprogramming by a well-established canonical signalling pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin, they associate with Aux/IAA transcriptional repressors and target them for degradation via ubiquitination
2
,
3
. Here we identify adenylate cyclase (AC) activity as an additional function of TIR1/AFB receptors across land plants. Auxin, together with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC motif of the TIR1 C-terminal region, all of which abolish the AC activity, each render TIR1 ineffective in mediating gravitropism and sustained auxin-induced root growth inhibition, and also affect auxin-induced transcriptional regulation. These results highlight the importance of TIR1/AFB AC activity in canonical auxin signalling. They also identify a unique phytohormone receptor cassette combining F-box and AC motifs, and the role of cAMP as a second messenger in plants.
Adenylate cyclase activity in TIR1/AFB, the canonical auxin receptor, has an essential role in auxin-mediated root growth inhibition in land plants.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/s41586-022-05369-7</identifier><identifier>PMID: 36289340</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14/19 ; 38/35 ; 38/77 ; 42/34 ; 631/449/1659 ; 631/449/1741/1576 ; 631/449/2491/2046 ; 631/449/2675 ; 82/1 ; 82/16 ; 82/58 ; 82/80 ; 82/83 ; 96/44 ; Adenylate cyclase ; Adenylyl Cyclases - genetics ; Adenylyl Cyclases - metabolism ; Arabidopsis - enzymology ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Auxins ; Cyclic AMP ; Cyclic AMP - metabolism ; E coli ; Enzyme kinetics ; F-Box Proteins - genetics ; F-Box Proteins - metabolism ; Gene Expression Regulation, Plant ; Gene regulation ; Gravitropism ; Humanities and Social Sciences ; Indoleacetic Acids - metabolism ; Indoleacetic Acids - pharmacology ; multidisciplinary ; Mutation ; Plant growth ; Plant Growth Regulators - metabolism ; Plant Growth Regulators - pharmacology ; Plant hormones ; Plant Roots - growth & development ; Proteins ; Receptor mechanisms ; Receptors ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - metabolism ; Repressors ; Science ; Science (multidisciplinary) ; Second Messenger Systems ; Signal transduction ; Signaling ; Transcription ; Transcription factors ; Ubiquitin ; Ubiquitin-protein ligase</subject><ispartof>Nature (London), 2022-11, Vol.611 (7934), p.133-138</ispartof><rights>The Author(s), under exclusive licence to Springer Nature Limited 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2022. The Author(s), under exclusive licence to Springer Nature Limited.</rights><rights>Copyright Nature Publishing Group Nov 3, 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-65273830eb30756d6c818048c2cccfe798c3739e6a1df5e214a139ea421cd4bb3</citedby><cites>FETCH-LOGICAL-c441t-65273830eb30756d6c818048c2cccfe798c3739e6a1df5e214a139ea421cd4bb3</cites><orcidid>0000-0002-0605-518X ; 0000-0002-1473-4545 ; 0000-0001-9958-017X ; 0000-0002-8597-268X ; 0000-0001-5187-8401 ; 0000-0002-4566-0593 ; 0000-0002-8302-7596</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/s41586-022-05369-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/s41586-022-05369-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36289340$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qi, Linlin</creatorcontrib><creatorcontrib>Kwiatkowski, Mateusz</creatorcontrib><creatorcontrib>Chen, Huihuang</creatorcontrib><creatorcontrib>Hoermayer, Lukas</creatorcontrib><creatorcontrib>Sinclair, Scott</creatorcontrib><creatorcontrib>Zou, Minxia</creatorcontrib><creatorcontrib>del Genio, Charo I.</creatorcontrib><creatorcontrib>Kubeš, Martin F.</creatorcontrib><creatorcontrib>Napier, Richard</creatorcontrib><creatorcontrib>Jaworski, Krzysztof</creatorcontrib><creatorcontrib>Friml, Jiří</creatorcontrib><title>Adenylate cyclase activity of TIR1/AFB auxin receptors in plants</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>The phytohormone auxin is the major coordinative signal in plant development
1
, mediating transcriptional reprogramming by a well-established canonical signalling pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin, they associate with Aux/IAA transcriptional repressors and target them for degradation via ubiquitination
2
,
3
. Here we identify adenylate cyclase (AC) activity as an additional function of TIR1/AFB receptors across land plants. Auxin, together with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC motif of the TIR1 C-terminal region, all of which abolish the AC activity, each render TIR1 ineffective in mediating gravitropism and sustained auxin-induced root growth inhibition, and also affect auxin-induced transcriptional regulation. These results highlight the importance of TIR1/AFB AC activity in canonical auxin signalling. They also identify a unique phytohormone receptor cassette combining F-box and AC motifs, and the role of cAMP as a second messenger in plants.
Adenylate cyclase activity in TIR1/AFB, the canonical auxin receptor, has an essential role in auxin-mediated root growth inhibition in land plants.</description><subject>14/19</subject><subject>38/35</subject><subject>38/77</subject><subject>42/34</subject><subject>631/449/1659</subject><subject>631/449/1741/1576</subject><subject>631/449/2491/2046</subject><subject>631/449/2675</subject><subject>82/1</subject><subject>82/16</subject><subject>82/58</subject><subject>82/80</subject><subject>82/83</subject><subject>96/44</subject><subject>Adenylate cyclase</subject><subject>Adenylyl Cyclases - genetics</subject><subject>Adenylyl Cyclases - metabolism</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Auxins</subject><subject>Cyclic AMP</subject><subject>Cyclic AMP - metabolism</subject><subject>E coli</subject><subject>Enzyme 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cyclase activity of TIR1/AFB auxin receptors in plants</title><author>Qi, Linlin ; Kwiatkowski, Mateusz ; Chen, Huihuang ; Hoermayer, Lukas ; Sinclair, Scott ; Zou, Minxia ; del Genio, Charo I. ; Kubeš, Martin F. ; Napier, Richard ; Jaworski, Krzysztof ; Friml, Jiří</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-65273830eb30756d6c818048c2cccfe798c3739e6a1df5e214a139ea421cd4bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>14/19</topic><topic>38/35</topic><topic>38/77</topic><topic>42/34</topic><topic>631/449/1659</topic><topic>631/449/1741/1576</topic><topic>631/449/2491/2046</topic><topic>631/449/2675</topic><topic>82/1</topic><topic>82/16</topic><topic>82/58</topic><topic>82/80</topic><topic>82/83</topic><topic>96/44</topic><topic>Adenylate cyclase</topic><topic>Adenylyl Cyclases - genetics</topic><topic>Adenylyl Cyclases - metabolism</topic><topic>Arabidopsis - enzymology</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Auxins</topic><topic>Cyclic AMP</topic><topic>Cyclic AMP - metabolism</topic><topic>E coli</topic><topic>Enzyme kinetics</topic><topic>F-Box Proteins - genetics</topic><topic>F-Box Proteins - metabolism</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene regulation</topic><topic>Gravitropism</topic><topic>Humanities and Social Sciences</topic><topic>Indoleacetic Acids - metabolism</topic><topic>Indoleacetic Acids - pharmacology</topic><topic>multidisciplinary</topic><topic>Mutation</topic><topic>Plant growth</topic><topic>Plant Growth Regulators - metabolism</topic><topic>Plant Growth Regulators - pharmacology</topic><topic>Plant hormones</topic><topic>Plant Roots - growth & development</topic><topic>Proteins</topic><topic>Receptor mechanisms</topic><topic>Receptors</topic><topic>Receptors, Cell Surface - 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of TIR1/AFB auxin receptors in plants</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>2022-11-03</date><risdate>2022</risdate><volume>611</volume><issue>7934</issue><spage>133</spage><epage>138</epage><pages>133-138</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>The phytohormone auxin is the major coordinative signal in plant development
1
, mediating transcriptional reprogramming by a well-established canonical signalling pathway. TRANSPORT INHIBITOR RESPONSE 1 (TIR1)/AUXIN-SIGNALING F-BOX (AFB) auxin receptors are F-box subunits of ubiquitin ligase complexes. In response to auxin, they associate with Aux/IAA transcriptional repressors and target them for degradation via ubiquitination
2
,
3
. Here we identify adenylate cyclase (AC) activity as an additional function of TIR1/AFB receptors across land plants. Auxin, together with Aux/IAAs, stimulates cAMP production. Three separate mutations in the AC motif of the TIR1 C-terminal region, all of which abolish the AC activity, each render TIR1 ineffective in mediating gravitropism and sustained auxin-induced root growth inhibition, and also affect auxin-induced transcriptional regulation. These results highlight the importance of TIR1/AFB AC activity in canonical auxin signalling. They also identify a unique phytohormone receptor cassette combining F-box and AC motifs, and the role of cAMP as a second messenger in plants.
Adenylate cyclase activity in TIR1/AFB, the canonical auxin receptor, has an essential role in auxin-mediated root growth inhibition in land plants.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>36289340</pmid><doi>10.1038/s41586-022-05369-7</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-0605-518X</orcidid><orcidid>https://orcid.org/0000-0002-1473-4545</orcidid><orcidid>https://orcid.org/0000-0001-9958-017X</orcidid><orcidid>https://orcid.org/0000-0002-8597-268X</orcidid><orcidid>https://orcid.org/0000-0001-5187-8401</orcidid><orcidid>https://orcid.org/0000-0002-4566-0593</orcidid><orcidid>https://orcid.org/0000-0002-8302-7596</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 2022-11, Vol.611 (7934), p.133-138 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2729521137 |
| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | 14/19 38/35 38/77 42/34 631/449/1659 631/449/1741/1576 631/449/2491/2046 631/449/2675 82/1 82/16 82/58 82/80 82/83 96/44 Adenylate cyclase Adenylyl Cyclases - genetics Adenylyl Cyclases - metabolism Arabidopsis - enzymology Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Auxins Cyclic AMP Cyclic AMP - metabolism E coli Enzyme kinetics F-Box Proteins - genetics F-Box Proteins - metabolism Gene Expression Regulation, Plant Gene regulation Gravitropism Humanities and Social Sciences Indoleacetic Acids - metabolism Indoleacetic Acids - pharmacology multidisciplinary Mutation Plant growth Plant Growth Regulators - metabolism Plant Growth Regulators - pharmacology Plant hormones Plant Roots - growth & development Proteins Receptor mechanisms Receptors Receptors, Cell Surface - genetics Receptors, Cell Surface - metabolism Repressors Science Science (multidisciplinary) Second Messenger Systems Signal transduction Signaling Transcription Transcription factors Ubiquitin Ubiquitin-protein ligase |
| title | Adenylate cyclase activity of TIR1/AFB auxin receptors in plants |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T23%3A03%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Adenylate%20cyclase%20activity%20of%20TIR1/AFB%20auxin%20receptors%20in%20plants&rft.jtitle=Nature%20(London)&rft.au=Qi,%20Linlin&rft.date=2022-11-03&rft.volume=611&rft.issue=7934&rft.spage=133&rft.epage=138&rft.pages=133-138&rft.issn=0028-0836&rft.eissn=1476-4687&rft_id=info:doi/10.1038/s41586-022-05369-7&rft_dat=%3Cproquest_cross%3E2731823023%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2731823023&rft_id=info:pmid/36289340&rfr_iscdi=true |