Application of Carbohydrases in Extracting Protein from Rice Bran
Enzymatic pretreatments of rice bran, using commercial carbohydrases to improve nitrogen extractability, were investigated. The pretreatment used a response surface method which included the variations in concentration of Viscozyme L (0–120 FBG unit) and Celluclast 1.5L (0–360 NC unit), time of incu...
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| Veröffentlicht in: | Journal of the science of food and agriculture 1997-06, Vol.74 (2), p.141-146 |
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| creator | Ansharullah Hourigan, James A Chesterman, Colin F |
| description | Enzymatic pretreatments of rice bran, using commercial carbohydrases to improve nitrogen extractability, were investigated. The pretreatment used a response surface method which included the variations in concentration of Viscozyme L (0–120 FBG unit) and Celluclast 1.5L (0–360 NC unit), time of incubation (1–5 h) and pH (3·8–5·4). The experiments were conducted at two different temperatures, 40 and 50°C. At 40°C, the maximum nitrogen extraction predicted by the model was obtained when the Viscozyme–Celluclast mixture at pH 3·8 with incubation time of 5 h was applied, giving an extracted N of 51%. At the process temperature of 50°C, the model predicted that the use of Viscozyme alone, under similar conditions, would have a most significant effect in increasing the efficacy of the nitrogen extraction, producing a 57% extracted N. This enzymatic pretreatment method improved the nitrogen extractability of rice bran at the neutral pH. © 1997 SCI. |
| doi_str_mv | 10.1002/(SICI)1097-0010(199706)74:2<141::AID-JSFA778>3.0.CO;2-5 |
| format | Article |
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The pretreatment used a response surface method which included the variations in concentration of Viscozyme L (0–120 FBG unit) and Celluclast 1.5L (0–360 NC unit), time of incubation (1–5 h) and pH (3·8–5·4). The experiments were conducted at two different temperatures, 40 and 50°C. At 40°C, the maximum nitrogen extraction predicted by the model was obtained when the Viscozyme–Celluclast mixture at pH 3·8 with incubation time of 5 h was applied, giving an extracted N of 51%. At the process temperature of 50°C, the model predicted that the use of Viscozyme alone, under similar conditions, would have a most significant effect in increasing the efficacy of the nitrogen extraction, producing a 57% extracted N. 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This enzymatic pretreatment method improved the nitrogen extractability of rice bran at the neutral pH. © 1997 SCI.</description><subject>Biological and medical sciences</subject><subject>carbohydrases</subject><subject>Cereal and baking product industries</subject><subject>concentrates</subject><subject>enzymatic method</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>protein extractability</topic><topic>rice bran</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ansharullah</creatorcontrib><creatorcontrib>Hourigan, James A</creatorcontrib><creatorcontrib>Chesterman, Colin F</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Periodicals Index Online Segment 19</collection><collection>Periodicals Index Online Segment 30</collection><collection>Periodicals Index Online</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - West</collection><collection>Primary Sources Access (Plan D) - International</collection><collection>Primary Sources Access & Build (Plan A) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Midwest</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Northeast</collection><collection>Primary Sources Access (Plan D) - Southeast</collection><collection>Primary Sources Access (Plan D) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Southeast</collection><collection>Primary Sources Access (Plan D) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - UK / I</collection><collection>Primary Sources Access (Plan D) - Canada</collection><collection>Primary Sources Access (Plan D) - EMEALA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - International</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - International</collection><collection>Primary Sources Access (Plan D) - West</collection><collection>Periodicals Index Online Segments 1-50</collection><collection>Primary Sources Access (Plan D) - APAC</collection><collection>Primary Sources Access (Plan D) - Midwest</collection><collection>Primary Sources Access (Plan D) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Canada</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - EMEALA</collection><collection>Primary Sources Access & Build (Plan A) - APAC</collection><collection>Primary Sources Access & Build (Plan A) - Canada</collection><collection>Primary Sources Access & Build (Plan A) - West</collection><collection>Primary Sources Access & Build (Plan A) - EMEALA</collection><collection>Primary Sources Access (Plan D) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - Midwest</collection><collection>Primary Sources Access & Build (Plan A) - North Central</collection><collection>Primary Sources Access & Build (Plan A) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - Southeast</collection><collection>Primary Sources Access (Plan D) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - APAC</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - MEA</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ansharullah</au><au>Hourigan, James A</au><au>Chesterman, Colin F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of Carbohydrases in Extracting Protein from Rice Bran</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J. 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At the process temperature of 50°C, the model predicted that the use of Viscozyme alone, under similar conditions, would have a most significant effect in increasing the efficacy of the nitrogen extraction, producing a 57% extracted N. This enzymatic pretreatment method improved the nitrogen extractability of rice bran at the neutral pH. © 1997 SCI.</abstract><cop>London</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/(SICI)1097-0010(199706)74:2<141::AID-JSFA778>3.0.CO;2-5</doi><tpages>6</tpages></addata></record> |
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| source | Periodicals Index Online; Access via Wiley Online Library |
| subjects | Biological and medical sciences carbohydrases Cereal and baking product industries concentrates enzymatic method Food industries Fundamental and applied biological sciences. Psychology protein extractability rice bran |
| title | Application of Carbohydrases in Extracting Protein from Rice Bran |
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