A comprehensive assessment of four whole blood stabilizers for flow‐cytometric analysis of leukocyte populations

Though cryopreservation of cell fractions is widely used in flow cytometry studies, whole blood cryopreservation is more challenging due to the presence of erythrocytes and effects of fixatives commonly used for preservation. Here, we evaluated and compared head‐to‐head the performance of four comme...

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Veröffentlicht in:	Cytometry. Part A 2023-04, Vol.103 (4), p.313-324
Hauptverfasser:	Nguyen, Ngoc Anh, Huang, Xiaobo, Cabrera, Luz E., Pekkarinen, Pirkka T., Nowlan, Kirsten, Strandin, Tomas, Kantele, Anu, Vapalahti, Olli, Heinonen, Santtu, Kekäläinen, Eliisa
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Blood CC chemokine receptors CCR7 protein CD11b antigen CD16 antigen COVID-19 Cryopreservation Design of experiments Erythrocytes Experimental design Fc receptors Fixatives Flow Cytometry Fluorescence Granulocytes Humans immunophenotyping L-selectin Leukocytes Leukocytes (granulocytic) Leukocytes (neutrophilic) Lipopolysaccharides Longitudinal studies Lymphocytes Lymphocytes B Lymphocytes T Lymphopenia method Natural killer cells Performance evaluation Phenotypes Phenylalanine Proteomics whole blood whole blood stabilizer
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