Cellulomonas dongxiuzhuiae sp. nov., Cellulomonas wangleii sp. nov. and Cellulomonas fengjieae sp. nov., isolated from the intestinal contents of Marmota himalayana
Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157 T /zg-ZUI40, zg-ZUI222 T /zg-ZUI199 and zg-ZUI188 T / zg-ZUI168) were characterized by a polyphasic approach. Optimal growth of the six strains was observed at p...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2022-10, Vol.72 (10) |
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| creator | Zhang, Gui Yang, Jing Lai, Xin-He Jin, Dong Lu, Shan Liu, Liyun Cheng, Yanpeng Pu, Ji Yang, Caixin Liu, Yue Ye, Lin Xu, Jianguo |
| description | Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157
T
/zg-ZUI40, zg-ZUI222
T
/zg-ZUI199 and zg-ZUI188
T
/ zg-ZUI168) were characterized by a polyphasic approach. Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus
Cellulomonas
. The three type strains have low digital DNA-DNA hybridization (19.3–30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus
Cellulomonas
, and a DNA G+C content range of 73.0-74.6 mol%. The major fatty acids detected in strain pairs zg-ZUI157
T
/zg-ZUI40 and zg-ZUI 222
T
/zg-ZUI199 were C
16:0
, anteiso-C
15:0
and anteiso A-C
15:1
, and C
16:0
, anteiso-C
15:0
, anteiso A-C
15:1
and anteiso-C
17:0
in strain pair zg-ZUI188
T
/zg-ZUI168. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species. MK-9(H
4
) was the predominant quinone detected in strains zg-ZUI222
T
(87.4 %) and zg-ZUI188
T
(91.4 %), and MK-9(H
4
) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157
T
. The cell-wall sugars detected in the three novel species mainly contained rhamnose. The cell-wall peptidoglycan type of the three novel species was A4
β
, with an inferred
l
-Orn
–d
-Asp interpeptide bridge for strains zg-ZUI157
T
and zg-ZUI222
T
, and
l
-Orn
–d
-Glu for strain zg-ZUI188
T
. Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel
Cellulomonas
species, for which the names
Cellulomonas dongxiuzhuiae
sp. nov. (zg-ZUI157
T
=GDMCC 1.2559
T
=KCTC 49678
T
),
Cellulomonas wangleii
sp. nov. (zg-ZUI222
T
=GDMCC 1.2501
T
=KCTC 49675
T
) and
Cellulomonas fengjieae
sp. nov. (zg-ZUI188
T
=GDMCC 1.2563
T
=KCTC 49674
T
) are proposed. |
| doi_str_mv | 10.1099/ijsem.0.005533 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2726922649</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2726922649</sourcerecordid><originalsourceid>FETCH-LOGICAL-c272t-8249ae414bfac9c66423f3d71cf3c84df066a348a9d5298ecdba9db3169cc9e13</originalsourceid><addsrcrecordid>eNpVkctOwzAQRS0EEqWwZe0lCxr8ihsvUcVLArGBtTV17NaVY5c4Acr38KEEiip1NVczRzN3dBE6p6SgRKkrv8q2KUhBSFlyfoBGVEg5KQkXhzvN5DE6yXlFyNAgZIS-ZzaEPqQmRci4TnHx6fuvZe_B4rwucEzvxSXegz4gLoL1fjfHEOt9xNm4WHm7t8PnFKCzNXZtanC3tNjHzubORwjYpEHHLuPk8BO0TeoAL30DATYQ4RQdOQjZnv3XMXq9vXmZ3U8en-8eZtePE8OmrJtUTCiwgoq5A6OMlIJxx-spNY6bStSOSAlcVKDqkqnKmno-yDmnUhmjLOVjdLHdu27TWz94043PZngMok191sMVqRiTQg1osUVNm3JurdPrdvDbbjQl-jcO_ReHJnobB_8Bc5eCpA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2726922649</pqid></control><display><type>article</type><title>Cellulomonas dongxiuzhuiae sp. nov., Cellulomonas wangleii sp. nov. and Cellulomonas fengjieae sp. nov., isolated from the intestinal contents of Marmota himalayana</title><source>Microbiology Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Zhang, Gui ; Yang, Jing ; Lai, Xin-He ; Jin, Dong ; Lu, Shan ; Liu, Liyun ; Cheng, Yanpeng ; Pu, Ji ; Yang, Caixin ; Liu, Yue ; Ye, Lin ; Xu, Jianguo</creator><creatorcontrib>Zhang, Gui ; Yang, Jing ; Lai, Xin-He ; Jin, Dong ; Lu, Shan ; Liu, Liyun ; Cheng, Yanpeng ; Pu, Ji ; Yang, Caixin ; Liu, Yue ; Ye, Lin ; Xu, Jianguo</creatorcontrib><description>Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157
T
/zg-ZUI40, zg-ZUI222
T
/zg-ZUI199 and zg-ZUI188
T
/ zg-ZUI168) were characterized by a polyphasic approach. Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus
Cellulomonas
. The three type strains have low digital DNA-DNA hybridization (19.3–30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus
Cellulomonas
, and a DNA G+C content range of 73.0-74.6 mol%. The major fatty acids detected in strain pairs zg-ZUI157
T
/zg-ZUI40 and zg-ZUI 222
T
/zg-ZUI199 were C
16:0
, anteiso-C
15:0
and anteiso A-C
15:1
, and C
16:0
, anteiso-C
15:0
, anteiso A-C
15:1
and anteiso-C
17:0
in strain pair zg-ZUI188
T
/zg-ZUI168. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species. MK-9(H
4
) was the predominant quinone detected in strains zg-ZUI222
T
(87.4 %) and zg-ZUI188
T
(91.4 %), and MK-9(H
4
) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157
T
. The cell-wall sugars detected in the three novel species mainly contained rhamnose. The cell-wall peptidoglycan type of the three novel species was A4
β
, with an inferred
l
-Orn
–d
-Asp interpeptide bridge for strains zg-ZUI157
T
and zg-ZUI222
T
, and
l
-Orn
–d
-Glu for strain zg-ZUI188
T
. Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel
Cellulomonas
species, for which the names
Cellulomonas dongxiuzhuiae
sp. nov. (zg-ZUI157
T
=GDMCC 1.2559
T
=KCTC 49678
T
),
Cellulomonas wangleii
sp. nov. (zg-ZUI222
T
=GDMCC 1.2501
T
=KCTC 49675
T
) and
Cellulomonas fengjieae
sp. nov. (zg-ZUI188
T
=GDMCC 1.2563
T
=KCTC 49674
T
) are proposed.</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.005533</identifier><language>eng</language><ispartof>International journal of systematic and evolutionary microbiology, 2022-10, Vol.72 (10)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-8249ae414bfac9c66423f3d71cf3c84df066a348a9d5298ecdba9db3169cc9e13</citedby><cites>FETCH-LOGICAL-c272t-8249ae414bfac9c66423f3d71cf3c84df066a348a9d5298ecdba9db3169cc9e13</cites><orcidid>0000-0003-3649-8229</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,27901,27902</link.rule.ids></links><search><creatorcontrib>Zhang, Gui</creatorcontrib><creatorcontrib>Yang, Jing</creatorcontrib><creatorcontrib>Lai, Xin-He</creatorcontrib><creatorcontrib>Jin, Dong</creatorcontrib><creatorcontrib>Lu, Shan</creatorcontrib><creatorcontrib>Liu, Liyun</creatorcontrib><creatorcontrib>Cheng, Yanpeng</creatorcontrib><creatorcontrib>Pu, Ji</creatorcontrib><creatorcontrib>Yang, Caixin</creatorcontrib><creatorcontrib>Liu, Yue</creatorcontrib><creatorcontrib>Ye, Lin</creatorcontrib><creatorcontrib>Xu, Jianguo</creatorcontrib><title>Cellulomonas dongxiuzhuiae sp. nov., Cellulomonas wangleii sp. nov. and Cellulomonas fengjieae sp. nov., isolated from the intestinal contents of Marmota himalayana</title><title>International journal of systematic and evolutionary microbiology</title><description>Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157
T
/zg-ZUI40, zg-ZUI222
T
/zg-ZUI199 and zg-ZUI188
T
/ zg-ZUI168) were characterized by a polyphasic approach. Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus
Cellulomonas
. The three type strains have low digital DNA-DNA hybridization (19.3–30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus
Cellulomonas
, and a DNA G+C content range of 73.0-74.6 mol%. The major fatty acids detected in strain pairs zg-ZUI157
T
/zg-ZUI40 and zg-ZUI 222
T
/zg-ZUI199 were C
16:0
, anteiso-C
15:0
and anteiso A-C
15:1
, and C
16:0
, anteiso-C
15:0
, anteiso A-C
15:1
and anteiso-C
17:0
in strain pair zg-ZUI188
T
/zg-ZUI168. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species. MK-9(H
4
) was the predominant quinone detected in strains zg-ZUI222
T
(87.4 %) and zg-ZUI188
T
(91.4 %), and MK-9(H
4
) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157
T
. The cell-wall sugars detected in the three novel species mainly contained rhamnose. The cell-wall peptidoglycan type of the three novel species was A4
β
, with an inferred
l
-Orn
–d
-Asp interpeptide bridge for strains zg-ZUI157
T
and zg-ZUI222
T
, and
l
-Orn
–d
-Glu for strain zg-ZUI188
T
. Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel
Cellulomonas
species, for which the names
Cellulomonas dongxiuzhuiae
sp. nov. (zg-ZUI157
T
=GDMCC 1.2559
T
=KCTC 49678
T
),
Cellulomonas wangleii
sp. nov. (zg-ZUI222
T
=GDMCC 1.2501
T
=KCTC 49675
T
) and
Cellulomonas fengjieae
sp. nov. (zg-ZUI188
T
=GDMCC 1.2563
T
=KCTC 49674
T
) are proposed.</description><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpVkctOwzAQRS0EEqWwZe0lCxr8ihsvUcVLArGBtTV17NaVY5c4Acr38KEEiip1NVczRzN3dBE6p6SgRKkrv8q2KUhBSFlyfoBGVEg5KQkXhzvN5DE6yXlFyNAgZIS-ZzaEPqQmRci4TnHx6fuvZe_B4rwucEzvxSXegz4gLoL1fjfHEOt9xNm4WHm7t8PnFKCzNXZtanC3tNjHzubORwjYpEHHLuPk8BO0TeoAL30DATYQ4RQdOQjZnv3XMXq9vXmZ3U8en-8eZtePE8OmrJtUTCiwgoq5A6OMlIJxx-spNY6bStSOSAlcVKDqkqnKmno-yDmnUhmjLOVjdLHdu27TWz94043PZngMok191sMVqRiTQg1osUVNm3JurdPrdvDbbjQl-jcO_ReHJnobB_8Bc5eCpA</recordid><startdate>20221001</startdate><enddate>20221001</enddate><creator>Zhang, Gui</creator><creator>Yang, Jing</creator><creator>Lai, Xin-He</creator><creator>Jin, Dong</creator><creator>Lu, Shan</creator><creator>Liu, Liyun</creator><creator>Cheng, Yanpeng</creator><creator>Pu, Ji</creator><creator>Yang, Caixin</creator><creator>Liu, Yue</creator><creator>Ye, Lin</creator><creator>Xu, Jianguo</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3649-8229</orcidid></search><sort><creationdate>20221001</creationdate><title>Cellulomonas dongxiuzhuiae sp. nov., Cellulomonas wangleii sp. nov. and Cellulomonas fengjieae sp. nov., isolated from the intestinal contents of Marmota himalayana</title><author>Zhang, Gui ; Yang, Jing ; Lai, Xin-He ; Jin, Dong ; Lu, Shan ; Liu, Liyun ; Cheng, Yanpeng ; Pu, Ji ; Yang, Caixin ; Liu, Yue ; Ye, Lin ; Xu, Jianguo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-8249ae414bfac9c66423f3d71cf3c84df066a348a9d5298ecdba9db3169cc9e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Gui</creatorcontrib><creatorcontrib>Yang, Jing</creatorcontrib><creatorcontrib>Lai, Xin-He</creatorcontrib><creatorcontrib>Jin, Dong</creatorcontrib><creatorcontrib>Lu, Shan</creatorcontrib><creatorcontrib>Liu, Liyun</creatorcontrib><creatorcontrib>Cheng, Yanpeng</creatorcontrib><creatorcontrib>Pu, Ji</creatorcontrib><creatorcontrib>Yang, Caixin</creatorcontrib><creatorcontrib>Liu, Yue</creatorcontrib><creatorcontrib>Ye, Lin</creatorcontrib><creatorcontrib>Xu, Jianguo</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Gui</au><au>Yang, Jing</au><au>Lai, Xin-He</au><au>Jin, Dong</au><au>Lu, Shan</au><au>Liu, Liyun</au><au>Cheng, Yanpeng</au><au>Pu, Ji</au><au>Yang, Caixin</au><au>Liu, Yue</au><au>Ye, Lin</au><au>Xu, Jianguo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellulomonas dongxiuzhuiae sp. nov., Cellulomonas wangleii sp. nov. and Cellulomonas fengjieae sp. nov., isolated from the intestinal contents of Marmota himalayana</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><date>2022-10-01</date><risdate>2022</risdate><volume>72</volume><issue>10</issue><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157
T
/zg-ZUI40, zg-ZUI222
T
/zg-ZUI199 and zg-ZUI188
T
/ zg-ZUI168) were characterized by a polyphasic approach. Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus
Cellulomonas
. The three type strains have low digital DNA-DNA hybridization (19.3–30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus
Cellulomonas
, and a DNA G+C content range of 73.0-74.6 mol%. The major fatty acids detected in strain pairs zg-ZUI157
T
/zg-ZUI40 and zg-ZUI 222
T
/zg-ZUI199 were C
16:0
, anteiso-C
15:0
and anteiso A-C
15:1
, and C
16:0
, anteiso-C
15:0
, anteiso A-C
15:1
and anteiso-C
17:0
in strain pair zg-ZUI188
T
/zg-ZUI168. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species. MK-9(H
4
) was the predominant quinone detected in strains zg-ZUI222
T
(87.4 %) and zg-ZUI188
T
(91.4 %), and MK-9(H
4
) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157
T
. The cell-wall sugars detected in the three novel species mainly contained rhamnose. The cell-wall peptidoglycan type of the three novel species was A4
β
, with an inferred
l
-Orn
–d
-Asp interpeptide bridge for strains zg-ZUI157
T
and zg-ZUI222
T
, and
l
-Orn
–d
-Glu for strain zg-ZUI188
T
. Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel
Cellulomonas
species, for which the names
Cellulomonas dongxiuzhuiae
sp. nov. (zg-ZUI157
T
=GDMCC 1.2559
T
=KCTC 49678
T
),
Cellulomonas wangleii
sp. nov. (zg-ZUI222
T
=GDMCC 1.2501
T
=KCTC 49675
T
) and
Cellulomonas fengjieae
sp. nov. (zg-ZUI188
T
=GDMCC 1.2563
T
=KCTC 49674
T
) are proposed.</abstract><doi>10.1099/ijsem.0.005533</doi><orcidid>https://orcid.org/0000-0003-3649-8229</orcidid></addata></record> |
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| identifier | ISSN: 1466-5026 |
| ispartof | International journal of systematic and evolutionary microbiology, 2022-10, Vol.72 (10) |
| issn | 1466-5026 1466-5034 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2726922649 |
| source | Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| title | Cellulomonas dongxiuzhuiae sp. nov., Cellulomonas wangleii sp. nov. and Cellulomonas fengjieae sp. nov., isolated from the intestinal contents of Marmota himalayana |
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