Preimplantation genetic testing for human blastocysts with potential parental contamination using a quantitative parental contamination test (qPCT): an evidence-based study
Is it possible to develop a quantitative method for detecting parental DNA contamination in conventional IVF using preimplantation genetic testing for aneuploidy (PGT-A)? In this study, a quantification method was established for the parental contamination test (qPCT), which ensured more reliable re...
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| Veröffentlicht in: | Reproductive biomedicine online 2023-01, Vol.46 (1), p.69-79 |
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| creator | Dong, Yunqiao Liu, Dun Zou, Yangyun Wan, Cheng Chen, Chuangqi Dong, Mei Huang, Yuqiang Huang, Cuiyu Weng, Huinan Zhu, Xiulan Wang, Fang Jiao, Shujing Liu, Na Lu, Sijia Zhang, Xiqian Liu, Fenghua |
| description | Is it possible to develop a quantitative method for detecting parental DNA contamination in conventional IVF using preimplantation genetic testing for aneuploidy (PGT-A)?
In this study, a quantification method was established for the parental contamination test (qPCT), which ensured more reliable results, and then verified its effectiveness for vitrified conventional IVF embryos. A total of 120 surplus vitrified blastocysts from patients who underwent prior routine IVF cycles were available for study.
The results of the prospective clinical study of qPCT-PGT-A showed that the maternal contamination rate was 0.83% (1/120) and that the risk of paternal contamination was negligible. The 24 frozen embryo transfer cycles resulted in 16 clinical pregnancies, including 13 live births, one late inevitable miscarriage and two ongoing pregnancies.
The risk of PGT in embryos with potential parental contamination is relatively low, and PGT-A is applicable for vitrified conventional IVF embryos. |
| doi_str_mv | 10.1016/j.rbmo.2022.08.103 |
| format | Article |
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In this study, a quantification method was established for the parental contamination test (qPCT), which ensured more reliable results, and then verified its effectiveness for vitrified conventional IVF embryos. A total of 120 surplus vitrified blastocysts from patients who underwent prior routine IVF cycles were available for study.
The results of the prospective clinical study of qPCT-PGT-A showed that the maternal contamination rate was 0.83% (1/120) and that the risk of paternal contamination was negligible. The 24 frozen embryo transfer cycles resulted in 16 clinical pregnancies, including 13 live births, one late inevitable miscarriage and two ongoing pregnancies.
The risk of PGT in embryos with potential parental contamination is relatively low, and PGT-A is applicable for vitrified conventional IVF embryos.</description><identifier>ISSN: 1472-6483</identifier><identifier>EISSN: 1472-6491</identifier><identifier>DOI: 10.1016/j.rbmo.2022.08.103</identifier><identifier>PMID: 36257886</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Aneuploidy ; Biopsy ; Blastocyst ; Fathers ; Female ; Fertilization in Vitro - methods ; Genetic Testing - methods ; Human blastocyst ; Humans ; IVF ; Male ; Parents ; PGT ; Pregnancy ; Preimplantation Diagnosis - methods ; Prospective Studies ; Quantitative parental contamination testing</subject><ispartof>Reproductive biomedicine online, 2023-01, Vol.46 (1), p.69-79</ispartof><rights>2022 The Authors</rights><rights>Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-1b8e54f70d88f84f7f500a8da94e1efcbd1a483c291e40fd32b0aeb5dcaa4f3e3</citedby><cites>FETCH-LOGICAL-c400t-1b8e54f70d88f84f7f500a8da94e1efcbd1a483c291e40fd32b0aeb5dcaa4f3e3</cites><orcidid>0000-0002-4186-2876 ; 0000-0002-3860-9466</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rbmo.2022.08.103$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36257886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dong, Yunqiao</creatorcontrib><creatorcontrib>Liu, Dun</creatorcontrib><creatorcontrib>Zou, Yangyun</creatorcontrib><creatorcontrib>Wan, Cheng</creatorcontrib><creatorcontrib>Chen, Chuangqi</creatorcontrib><creatorcontrib>Dong, Mei</creatorcontrib><creatorcontrib>Huang, Yuqiang</creatorcontrib><creatorcontrib>Huang, Cuiyu</creatorcontrib><creatorcontrib>Weng, Huinan</creatorcontrib><creatorcontrib>Zhu, Xiulan</creatorcontrib><creatorcontrib>Wang, Fang</creatorcontrib><creatorcontrib>Jiao, Shujing</creatorcontrib><creatorcontrib>Liu, Na</creatorcontrib><creatorcontrib>Lu, Sijia</creatorcontrib><creatorcontrib>Zhang, Xiqian</creatorcontrib><creatorcontrib>Liu, Fenghua</creatorcontrib><title>Preimplantation genetic testing for human blastocysts with potential parental contamination using a quantitative parental contamination test (qPCT): an evidence-based study</title><title>Reproductive biomedicine online</title><addtitle>Reprod Biomed Online</addtitle><description>Is it possible to develop a quantitative method for detecting parental DNA contamination in conventional IVF using preimplantation genetic testing for aneuploidy (PGT-A)?
In this study, a quantification method was established for the parental contamination test (qPCT), which ensured more reliable results, and then verified its effectiveness for vitrified conventional IVF embryos. A total of 120 surplus vitrified blastocysts from patients who underwent prior routine IVF cycles were available for study.
The results of the prospective clinical study of qPCT-PGT-A showed that the maternal contamination rate was 0.83% (1/120) and that the risk of paternal contamination was negligible. The 24 frozen embryo transfer cycles resulted in 16 clinical pregnancies, including 13 live births, one late inevitable miscarriage and two ongoing pregnancies.
The risk of PGT in embryos with potential parental contamination is relatively low, and PGT-A is applicable for vitrified conventional IVF embryos.</description><subject>Aneuploidy</subject><subject>Biopsy</subject><subject>Blastocyst</subject><subject>Fathers</subject><subject>Female</subject><subject>Fertilization in Vitro - methods</subject><subject>Genetic Testing - methods</subject><subject>Human blastocyst</subject><subject>Humans</subject><subject>IVF</subject><subject>Male</subject><subject>Parents</subject><subject>PGT</subject><subject>Pregnancy</subject><subject>Preimplantation Diagnosis - methods</subject><subject>Prospective Studies</subject><subject>Quantitative parental contamination testing</subject><issn>1472-6483</issn><issn>1472-6491</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uctu2zAQJIoUzfMHcgh4TA9yl5QsU0EugdG0BQI0h_RMUOQyoSGJMkm58D_1I0vBaW7paQeL2RlyhpBLBgsGrP6yWYS29wsOnC9A5F35gZywasWLumrY0RsW5TE5jXEDwASI8hM5Lmu-XAlRn5A_jwFdP3ZqSCo5P9BnHDA5TRPG5IZnan2gL1OvBtp2Kiav9zFF-tulFzr6hENyqqOjChlloH0evRsOWlOcFRTdTlnezQY7fI87-9Hr7eP66fMNzW64cwYHjUWrIhoa02T25-SjVV3Ei9d5Rn7df31afy8efn77sb57KHQFkArWClxWdgVGCCsysEsAJYxqKmRodWuYyqFo3jCswJqSt6CwXRqtVGVLLM_I9UF3DH475YfJ3kWNXU4J_RQlX_G6gobXTabyA1UHH2NAK8fgehX2koGcW5IbObck55YkiLwr89HVq_7U9mjeTv7Vkgm3BwLmX-4cBhm1m9MwLqBO0nj3P_2_cL-p7g</recordid><startdate>202301</startdate><enddate>202301</enddate><creator>Dong, Yunqiao</creator><creator>Liu, Dun</creator><creator>Zou, Yangyun</creator><creator>Wan, Cheng</creator><creator>Chen, Chuangqi</creator><creator>Dong, Mei</creator><creator>Huang, Yuqiang</creator><creator>Huang, Cuiyu</creator><creator>Weng, Huinan</creator><creator>Zhu, Xiulan</creator><creator>Wang, Fang</creator><creator>Jiao, Shujing</creator><creator>Liu, Na</creator><creator>Lu, Sijia</creator><creator>Zhang, Xiqian</creator><creator>Liu, Fenghua</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4186-2876</orcidid><orcidid>https://orcid.org/0000-0002-3860-9466</orcidid></search><sort><creationdate>202301</creationdate><title>Preimplantation genetic testing for human blastocysts with potential parental contamination using a quantitative parental contamination test (qPCT): an evidence-based study</title><author>Dong, Yunqiao ; Liu, Dun ; Zou, Yangyun ; Wan, Cheng ; Chen, Chuangqi ; Dong, Mei ; Huang, Yuqiang ; Huang, Cuiyu ; Weng, Huinan ; Zhu, Xiulan ; Wang, Fang ; Jiao, Shujing ; Liu, Na ; Lu, Sijia ; Zhang, Xiqian ; Liu, Fenghua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-1b8e54f70d88f84f7f500a8da94e1efcbd1a483c291e40fd32b0aeb5dcaa4f3e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Aneuploidy</topic><topic>Biopsy</topic><topic>Blastocyst</topic><topic>Fathers</topic><topic>Female</topic><topic>Fertilization in Vitro - methods</topic><topic>Genetic Testing - methods</topic><topic>Human blastocyst</topic><topic>Humans</topic><topic>IVF</topic><topic>Male</topic><topic>Parents</topic><topic>PGT</topic><topic>Pregnancy</topic><topic>Preimplantation Diagnosis - methods</topic><topic>Prospective Studies</topic><topic>Quantitative parental contamination testing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dong, Yunqiao</creatorcontrib><creatorcontrib>Liu, Dun</creatorcontrib><creatorcontrib>Zou, Yangyun</creatorcontrib><creatorcontrib>Wan, Cheng</creatorcontrib><creatorcontrib>Chen, Chuangqi</creatorcontrib><creatorcontrib>Dong, Mei</creatorcontrib><creatorcontrib>Huang, Yuqiang</creatorcontrib><creatorcontrib>Huang, Cuiyu</creatorcontrib><creatorcontrib>Weng, Huinan</creatorcontrib><creatorcontrib>Zhu, Xiulan</creatorcontrib><creatorcontrib>Wang, Fang</creatorcontrib><creatorcontrib>Jiao, Shujing</creatorcontrib><creatorcontrib>Liu, Na</creatorcontrib><creatorcontrib>Lu, Sijia</creatorcontrib><creatorcontrib>Zhang, Xiqian</creatorcontrib><creatorcontrib>Liu, Fenghua</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Reproductive biomedicine online</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dong, Yunqiao</au><au>Liu, Dun</au><au>Zou, Yangyun</au><au>Wan, Cheng</au><au>Chen, Chuangqi</au><au>Dong, Mei</au><au>Huang, Yuqiang</au><au>Huang, Cuiyu</au><au>Weng, Huinan</au><au>Zhu, Xiulan</au><au>Wang, Fang</au><au>Jiao, Shujing</au><au>Liu, Na</au><au>Lu, Sijia</au><au>Zhang, Xiqian</au><au>Liu, Fenghua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preimplantation genetic testing for human blastocysts with potential parental contamination using a quantitative parental contamination test (qPCT): an evidence-based study</atitle><jtitle>Reproductive biomedicine online</jtitle><addtitle>Reprod Biomed Online</addtitle><date>2023-01</date><risdate>2023</risdate><volume>46</volume><issue>1</issue><spage>69</spage><epage>79</epage><pages>69-79</pages><issn>1472-6483</issn><eissn>1472-6491</eissn><abstract>Is it possible to develop a quantitative method for detecting parental DNA contamination in conventional IVF using preimplantation genetic testing for aneuploidy (PGT-A)?
In this study, a quantification method was established for the parental contamination test (qPCT), which ensured more reliable results, and then verified its effectiveness for vitrified conventional IVF embryos. A total of 120 surplus vitrified blastocysts from patients who underwent prior routine IVF cycles were available for study.
The results of the prospective clinical study of qPCT-PGT-A showed that the maternal contamination rate was 0.83% (1/120) and that the risk of paternal contamination was negligible. The 24 frozen embryo transfer cycles resulted in 16 clinical pregnancies, including 13 live births, one late inevitable miscarriage and two ongoing pregnancies.
The risk of PGT in embryos with potential parental contamination is relatively low, and PGT-A is applicable for vitrified conventional IVF embryos.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>36257886</pmid><doi>10.1016/j.rbmo.2022.08.103</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4186-2876</orcidid><orcidid>https://orcid.org/0000-0002-3860-9466</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Reproductive biomedicine online, 2023-01, Vol.46 (1), p.69-79 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Aneuploidy Biopsy Blastocyst Fathers Female Fertilization in Vitro - methods Genetic Testing - methods Human blastocyst Humans IVF Male Parents PGT Pregnancy Preimplantation Diagnosis - methods Prospective Studies Quantitative parental contamination testing |
| title | Preimplantation genetic testing for human blastocysts with potential parental contamination using a quantitative parental contamination test (qPCT): an evidence-based study |
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