Antibacterial effect, cytotoxicity, and bond strength of a modified dental adhesive containing silver nanoparticles
[Abstract] This study aimed to evaluate the antibacterial effect, cytotoxicity, and microtensile bond strength of an adhesive system containing silver nanoparticles (NAg). NAg was synthesized and incorporated (500 and 1000 ppm) into Scotchbond Multi-Purpose (SBMP) primer and bond. A microtensile bon...
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| Veröffentlicht in: | Odontology 2023-04, Vol.111 (2), p.420-427 |
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| creator | Aguiar, Juliana Dias Pedrosa, Marlus da Silva Toma, Sergio Hiroshi Araki, Koiti Marques, Marcia Martins Medeiros, Igor Studart |
| description | [Abstract] This study aimed to evaluate the antibacterial effect, cytotoxicity, and microtensile bond strength of an adhesive system containing silver nanoparticles (NAg). NAg was synthesized and incorporated (500 and 1000 ppm) into Scotchbond Multi-Purpose (SBMP) primer and bond. A microtensile bond test (μTBS) was performed after 24 h and 1 year. The adhesive interface was characterized using a confocal Raman microscope. The antibacterial activity was assessed using agar diffusion and biofilm inhibition assays (S. mutans). MTT assay was used to assess the cytotoxicity of NAg-conditioned culture media on human dental pulp stem cells (hDPSCs). The results were statistically analyzed using analysis of variance and Tukey's tests (α = .01). Incorporating 500 and 1000 ppm of NAg in the SBMP did not affect the μTBS after 24 h (p > 0.05). However, in the 1 year evaluation, 500 ppm presented the highest μTBS values (p < 0.05). The addition of NAg at 500 and 1000 ppm in the primer and bond led to larger inhibition halos and colony-forming units than the control (p < 0.05). For the unpolymerized and polymerized groups, the combination of primer and bond presented the highest cytotoxic effects on hDPSCs (p < 0.05). In conclusion, incorporating 500 or 1000 ppm of NAg into an etch-and-rinse adhesive system led to an antibacterial effect without altering the cytotoxicity. SBMP at 500 ppm presented a higher μTBS at 1 year. |
| doi_str_mv | 10.1007/s10266-022-00752-2 |
| format | Article |
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NAg was synthesized and incorporated (500 and 1000 ppm) into Scotchbond Multi-Purpose (SBMP) primer and bond. A microtensile bond test (μTBS) was performed after 24 h and 1 year. The adhesive interface was characterized using a confocal Raman microscope. The antibacterial activity was assessed using agar diffusion and biofilm inhibition assays (S. mutans). MTT assay was used to assess the cytotoxicity of NAg-conditioned culture media on human dental pulp stem cells (hDPSCs). The results were statistically analyzed using analysis of variance and Tukey's tests (α = .01). Incorporating 500 and 1000 ppm of NAg in the SBMP did not affect the μTBS after 24 h (p > 0.05). However, in the 1 year evaluation, 500 ppm presented the highest μTBS values (p < 0.05). The addition of NAg at 500 and 1000 ppm in the primer and bond led to larger inhibition halos and colony-forming units than the control (p < 0.05). For the unpolymerized and polymerized groups, the combination of primer and bond presented the highest cytotoxic effects on hDPSCs (p < 0.05). In conclusion, incorporating 500 or 1000 ppm of NAg into an etch-and-rinse adhesive system led to an antibacterial effect without altering the cytotoxicity. SBMP at 500 ppm presented a higher μTBS at 1 year.</description><identifier>ISSN: 1618-1247</identifier><identifier>EISSN: 1618-1255</identifier><identifier>DOI: 10.1007/s10266-022-00752-2</identifier><identifier>PMID: 36209305</identifier><language>eng</language><publisher>Singapore: The Society of the Nippon Dental University</publisher><subject>Adhesives ; Anti-Bacterial Agents - pharmacology ; Antibacterial activity ; Biofilms ; Cell culture ; Culture media ; Cytotoxicity ; Dental Bonding ; Dental Cements - chemistry ; Dental Cements - pharmacology ; Dental pulp ; Dentin ; Dentin-Bonding Agents - chemistry ; Dentin-Bonding Agents - pharmacology ; Dentistry ; Humans ; Materials Testing ; Medicine ; Metal Nanoparticles - chemistry ; Nanoparticles ; Oral and Maxillofacial Surgery ; Original Article ; Resin Cements - chemistry ; Resin Cements - pharmacology ; Silver ; Silver - chemistry ; Silver - pharmacology ; Tensile Strength</subject><ispartof>Odontology, 2023-04, Vol.111 (2), p.420-427</ispartof><rights>The Author(s), under exclusive licence to The Society of The Nippon Dental University 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2022. The Author(s), under exclusive licence to The Society of The Nippon Dental University.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-fbb7032948b0a720705c0ee3b1bded481c6d2c72847eed0c289b7678de1a0d03</citedby><cites>FETCH-LOGICAL-c463t-fbb7032948b0a720705c0ee3b1bded481c6d2c72847eed0c289b7678de1a0d03</cites><orcidid>0000-0002-4052-7208</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10266-022-00752-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10266-022-00752-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36209305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aguiar, Juliana Dias</creatorcontrib><creatorcontrib>Pedrosa, Marlus da Silva</creatorcontrib><creatorcontrib>Toma, Sergio Hiroshi</creatorcontrib><creatorcontrib>Araki, Koiti</creatorcontrib><creatorcontrib>Marques, Marcia Martins</creatorcontrib><creatorcontrib>Medeiros, Igor Studart</creatorcontrib><creatorcontrib>Department of Microbiology & Immunology</creatorcontrib><creatorcontrib>Department of Biomaterials and Oral Biology</creatorcontrib><creatorcontrib>Chemical Institute</creatorcontrib><creatorcontrib>School of Dentistry of the University of Sao Paulo</creatorcontrib><creatorcontrib>The University of North Carolina at Chapel Hill</creatorcontrib><creatorcontrib>Post-Graduation Program in Dentistry</creatorcontrib><creatorcontrib>University of Sao Paulo</creatorcontrib><creatorcontrib>School of Dentistry of the Ibirapuera University</creatorcontrib><title>Antibacterial effect, cytotoxicity, and bond strength of a modified dental adhesive containing silver nanoparticles</title><title>Odontology</title><addtitle>Odontology</addtitle><addtitle>Odontology</addtitle><description>[Abstract] This study aimed to evaluate the antibacterial effect, cytotoxicity, and microtensile bond strength of an adhesive system containing silver nanoparticles (NAg). NAg was synthesized and incorporated (500 and 1000 ppm) into Scotchbond Multi-Purpose (SBMP) primer and bond. A microtensile bond test (μTBS) was performed after 24 h and 1 year. The adhesive interface was characterized using a confocal Raman microscope. The antibacterial activity was assessed using agar diffusion and biofilm inhibition assays (S. mutans). MTT assay was used to assess the cytotoxicity of NAg-conditioned culture media on human dental pulp stem cells (hDPSCs). The results were statistically analyzed using analysis of variance and Tukey's tests (α = .01). Incorporating 500 and 1000 ppm of NAg in the SBMP did not affect the μTBS after 24 h (p > 0.05). However, in the 1 year evaluation, 500 ppm presented the highest μTBS values (p < 0.05). The addition of NAg at 500 and 1000 ppm in the primer and bond led to larger inhibition halos and colony-forming units than the control (p < 0.05). For the unpolymerized and polymerized groups, the combination of primer and bond presented the highest cytotoxic effects on hDPSCs (p < 0.05). In conclusion, incorporating 500 or 1000 ppm of NAg into an etch-and-rinse adhesive system led to an antibacterial effect without altering the cytotoxicity. SBMP at 500 ppm presented a higher μTBS at 1 year.</description><subject>Adhesives</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial activity</subject><subject>Biofilms</subject><subject>Cell culture</subject><subject>Culture media</subject><subject>Cytotoxicity</subject><subject>Dental Bonding</subject><subject>Dental Cements - chemistry</subject><subject>Dental Cements - pharmacology</subject><subject>Dental pulp</subject><subject>Dentin</subject><subject>Dentin-Bonding Agents - chemistry</subject><subject>Dentin-Bonding Agents - pharmacology</subject><subject>Dentistry</subject><subject>Humans</subject><subject>Materials Testing</subject><subject>Medicine</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Nanoparticles</subject><subject>Oral and Maxillofacial Surgery</subject><subject>Original Article</subject><subject>Resin Cements - chemistry</subject><subject>Resin Cements - pharmacology</subject><subject>Silver</subject><subject>Silver - chemistry</subject><subject>Silver - pharmacology</subject><subject>Tensile Strength</subject><issn>1618-1247</issn><issn>1618-1255</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kk9v1DAQxSMEolXpF-CALHHh0MB4nMTJsaqgVKrEpXfLfyZbo6y92N6K_fZ4SWklDr3MeOT33lj6uWnec_jMAeSXzAGHoQXEto49tviqOeUDH1uOff_66dzJk-Y8Z2-gQ85B8v5tcyIGhElAf9rky1C80bZQ8nphNM9kywWzhxJL_O2tL4cLpoNjJtaSS6KwKfcszkyzbXR-9uSYo1CqWbt7yv6BmI119sGHDct-eaDEgg5xp1PxdqH8rnkz6yXT-WM_a-6-fb27-t7e_ri-ubq8bW03iNLOxkgQOHWjAS0RJPQWiIThxpHrRm4Hh1bi2EkiBxbHychBjo64BgfirPm0xu5S_LWnXNTWZ0vLogPFfVYoUfABhBRV-vE_6c-4T6E-rqpGOYiec1lVuKpsijknmtUu-a1OB8VBHaGoFYqqUNRfKAqr6cNj9N5syT1Z_iGoArEKcr0KG0rPu1-MvV5dNdNbvcSw-EDPVrvh0VUMCgGFAl7R47Gp-g3gWGTtOPWT-APZ2q5s</recordid><startdate>20230401</startdate><enddate>20230401</enddate><creator>Aguiar, Juliana Dias</creator><creator>Pedrosa, Marlus da Silva</creator><creator>Toma, Sergio Hiroshi</creator><creator>Araki, Koiti</creator><creator>Marques, Marcia Martins</creator><creator>Medeiros, Igor Studart</creator><general>The Society of the Nippon Dental University</general><general>Springer Nature Singapore</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4052-7208</orcidid></search><sort><creationdate>20230401</creationdate><title>Antibacterial effect, cytotoxicity, and bond strength of a modified dental adhesive containing silver nanoparticles</title><author>Aguiar, Juliana Dias ; Pedrosa, Marlus da Silva ; Toma, Sergio Hiroshi ; Araki, Koiti ; Marques, Marcia Martins ; Medeiros, Igor Studart</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-fbb7032948b0a720705c0ee3b1bded481c6d2c72847eed0c289b7678de1a0d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adhesives</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterial activity</topic><topic>Biofilms</topic><topic>Cell culture</topic><topic>Culture media</topic><topic>Cytotoxicity</topic><topic>Dental Bonding</topic><topic>Dental Cements - chemistry</topic><topic>Dental Cements - pharmacology</topic><topic>Dental pulp</topic><topic>Dentin</topic><topic>Dentin-Bonding Agents - chemistry</topic><topic>Dentin-Bonding Agents - pharmacology</topic><topic>Dentistry</topic><topic>Humans</topic><topic>Materials Testing</topic><topic>Medicine</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Nanoparticles</topic><topic>Oral and Maxillofacial Surgery</topic><topic>Original Article</topic><topic>Resin Cements - chemistry</topic><topic>Resin Cements - pharmacology</topic><topic>Silver</topic><topic>Silver - chemistry</topic><topic>Silver - pharmacology</topic><topic>Tensile Strength</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aguiar, Juliana Dias</creatorcontrib><creatorcontrib>Pedrosa, Marlus da Silva</creatorcontrib><creatorcontrib>Toma, Sergio Hiroshi</creatorcontrib><creatorcontrib>Araki, Koiti</creatorcontrib><creatorcontrib>Marques, Marcia Martins</creatorcontrib><creatorcontrib>Medeiros, Igor Studart</creatorcontrib><creatorcontrib>Department of Microbiology & Immunology</creatorcontrib><creatorcontrib>Department of Biomaterials and Oral Biology</creatorcontrib><creatorcontrib>Chemical Institute</creatorcontrib><creatorcontrib>School of Dentistry of the University of Sao Paulo</creatorcontrib><creatorcontrib>The University of North Carolina at Chapel Hill</creatorcontrib><creatorcontrib>Post-Graduation Program in Dentistry</creatorcontrib><creatorcontrib>University of Sao Paulo</creatorcontrib><creatorcontrib>School of Dentistry of the Ibirapuera University</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Odontology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aguiar, Juliana Dias</au><au>Pedrosa, Marlus da Silva</au><au>Toma, Sergio Hiroshi</au><au>Araki, Koiti</au><au>Marques, Marcia Martins</au><au>Medeiros, Igor Studart</au><aucorp>Department of Microbiology & Immunology</aucorp><aucorp>Department of Biomaterials and Oral Biology</aucorp><aucorp>Chemical Institute</aucorp><aucorp>School of Dentistry of the University of Sao Paulo</aucorp><aucorp>The University of North Carolina at Chapel Hill</aucorp><aucorp>Post-Graduation Program in Dentistry</aucorp><aucorp>University of Sao Paulo</aucorp><aucorp>School of Dentistry of the Ibirapuera University</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial effect, cytotoxicity, and bond strength of a modified dental adhesive containing silver nanoparticles</atitle><jtitle>Odontology</jtitle><stitle>Odontology</stitle><addtitle>Odontology</addtitle><date>2023-04-01</date><risdate>2023</risdate><volume>111</volume><issue>2</issue><spage>420</spage><epage>427</epage><pages>420-427</pages><issn>1618-1247</issn><eissn>1618-1255</eissn><abstract>[Abstract] This study aimed to evaluate the antibacterial effect, cytotoxicity, and microtensile bond strength of an adhesive system containing silver nanoparticles (NAg). NAg was synthesized and incorporated (500 and 1000 ppm) into Scotchbond Multi-Purpose (SBMP) primer and bond. A microtensile bond test (μTBS) was performed after 24 h and 1 year. The adhesive interface was characterized using a confocal Raman microscope. The antibacterial activity was assessed using agar diffusion and biofilm inhibition assays (S. mutans). MTT assay was used to assess the cytotoxicity of NAg-conditioned culture media on human dental pulp stem cells (hDPSCs). The results were statistically analyzed using analysis of variance and Tukey's tests (α = .01). Incorporating 500 and 1000 ppm of NAg in the SBMP did not affect the μTBS after 24 h (p > 0.05). However, in the 1 year evaluation, 500 ppm presented the highest μTBS values (p < 0.05). The addition of NAg at 500 and 1000 ppm in the primer and bond led to larger inhibition halos and colony-forming units than the control (p < 0.05). For the unpolymerized and polymerized groups, the combination of primer and bond presented the highest cytotoxic effects on hDPSCs (p < 0.05). In conclusion, incorporating 500 or 1000 ppm of NAg into an etch-and-rinse adhesive system led to an antibacterial effect without altering the cytotoxicity. SBMP at 500 ppm presented a higher μTBS at 1 year.</abstract><cop>Singapore</cop><pub>The Society of the Nippon Dental University</pub><pmid>36209305</pmid><doi>10.1007/s10266-022-00752-2</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-4052-7208</orcidid></addata></record> |
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| subjects | Adhesives Anti-Bacterial Agents - pharmacology Antibacterial activity Biofilms Cell culture Culture media Cytotoxicity Dental Bonding Dental Cements - chemistry Dental Cements - pharmacology Dental pulp Dentin Dentin-Bonding Agents - chemistry Dentin-Bonding Agents - pharmacology Dentistry Humans Materials Testing Medicine Metal Nanoparticles - chemistry Nanoparticles Oral and Maxillofacial Surgery Original Article Resin Cements - chemistry Resin Cements - pharmacology Silver Silver - chemistry Silver - pharmacology Tensile Strength |
| title | Antibacterial effect, cytotoxicity, and bond strength of a modified dental adhesive containing silver nanoparticles |
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