Gatekeeper Residue Replacement in a Phosphite Transporter Enhances Mutational Robustness of the Biocontainment Strategy

Biocontainment is a key methodology to reduce environmental risk through the deliberate release of genetically modified microorganisms. Previously, we developed a phosphite (HPO3 2–)-dependent biocontainment strategy, by expressing a phosphite-specific transporter HtxBCDE and phosphite dehydrogenase...

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Veröffentlicht in:	ACS synthetic biology 2022-10, Vol.11 (10), p.3397-3404
Hauptverfasser:	Hirota, Ryuichi, Katsuura, Zen-ichiro, Momokawa, Naoki, Murakami, Hiroki, Watanabe, Satoru, Ishida, Takenori, Ikeda, Takeshi, Funabashi, Hisakage, Kuroda, Akio
Format:	Artikel
Sprache:	eng
Schlagworte:	ATP-Binding Cassette Transporters - genetics Bacterial Proteins - metabolism Cysteine Escherichia coli - genetics Escherichia coli - metabolism Mutation Phenylalanine - genetics Phosphates - metabolism Phosphites - metabolism Phosphorus - metabolism Serine - genetics
Online-Zugang:	Volltext
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