Automated assays for trace elements and ferritin measurement in saliva of pigs: Analytical validation and a pilot application to evaluate different iron status
The aim of this study was to validate automated methods to measure iron (Fe), zinc (Zn), copper (Cu) and ferritin in pig saliva samples. A complete analytical validation was performed of all assays. In addition, these methods were applied to saliva of Fe supplemented (n = 22) and non-supplemented (n...
Gespeichert in:
| Veröffentlicht in: | Research in veterinary science 2022-12, Vol.152, p.410-416 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 416 |
|---|---|
| container_issue | |
| container_start_page | 410 |
| container_title | Research in veterinary science |
| container_volume | 152 |
| creator | Ortín-Bustillo, A. Vidal, G. Ramis Tortosa, D. Escribano López-Arjona, M. Rubio, C. Peres Cerón, J.J. Muñoz-Prieto, A. Pardo-Marín, L. López-Martínez, M.J. Botia, M. Martínez-Subiela, S. Tvarijonaviciute, A. Tecles, F. |
| description | The aim of this study was to validate automated methods to measure iron (Fe), zinc (Zn), copper (Cu) and ferritin in pig saliva samples. A complete analytical validation was performed of all assays. In addition, these methods were applied to saliva of Fe supplemented (n = 22) and non-supplemented (n = 20) piglets. All assays were able to measure these biomarkers in pig saliva with adequate precision, accuracy and high sensitivity and, in case of trace elements without needing a deproteinization pre-process. The group of piglets supplemented with Fe presented significantly higher levels of ferritin and Zn in saliva. In conclusion, the automated assays evaluated were able to measure Fe, Zn, Cu and ferritin in saliva of pigs, and in case of trace elements, they have the advantage of not needing a deproteinization pre-treatment and thus these analytes can be measured in a simple and fast manner.
•Iron (Fe), zinc (Zn) and copper (Cu) were analysed by automated assays in pig saliva.•Selected trace elements were measured reliably without pre-treatment in saliva.•Ferritin can be measured in pig saliva.•Ferritin and Zinc in saliva increased in pigs after iron supplementation. |
| doi_str_mv | 10.1016/j.rvsc.2022.09.007 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2723107950</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0034528822002880</els_id><sourcerecordid>2729073664</sourcerecordid><originalsourceid>FETCH-LOGICAL-c368t-aa92a9a2f3963adb91b973c442614fe66519ae974b71e127a02bd595212f2e083</originalsourceid><addsrcrecordid>eNqNkU-r1DAUxYsoOD79Aq4Cbty05k-bTMTN8Hj-gQdudB3upLeSoW1qbjown8avaqbjyoW4Csn5nXPDPVX1WvBGcKHfnZp0Jt9ILmXDbcO5eVLtRKdkLbUWT6sd56qtO7nfP69eEJ04560QZlf9Oqw5TpCxZ0AEF2JDTCwn8MhwxAnnTAzmng2YUshhZhMCrWlTWLkSjOEMLA5sCT_oPTvMMF5y8DCyc5F6yCHOWwIUYoyZwbKMRd_ec2RYsLV8gPVhKEO22FQkypBXelk9G2AkfPXnvKu-f3z4dv-5fvz66cv94bH2Su9zDWAlWJCDslpBf7TiaI3ybSu1aAfUuhMW0Jr2aAQKaYDLY9_ZTgo5SOR7dVe9veUuKf5ckbKbAnkcR5gxruSkkUpwYzv-H6jojFWmawv65i_0FNdUNrQFWm6U1ldK3iifIlHCwS0pTJAuTnB3rded3LVed63XcetKvcX04WbCspVzwOTIB5w99iGhz66P4V_23-4lsGM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2729073664</pqid></control><display><type>article</type><title>Automated assays for trace elements and ferritin measurement in saliva of pigs: Analytical validation and a pilot application to evaluate different iron status</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><creator>Ortín-Bustillo, A. ; Vidal, G. Ramis ; Tortosa, D. Escribano ; López-Arjona, M. ; Rubio, C. Peres ; Cerón, J.J. ; Muñoz-Prieto, A. ; Pardo-Marín, L. ; López-Martínez, M.J. ; Botia, M. ; Martínez-Subiela, S. ; Tvarijonaviciute, A. ; Tecles, F.</creator><creatorcontrib>Ortín-Bustillo, A. ; Vidal, G. Ramis ; Tortosa, D. Escribano ; López-Arjona, M. ; Rubio, C. Peres ; Cerón, J.J. ; Muñoz-Prieto, A. ; Pardo-Marín, L. ; López-Martínez, M.J. ; Botia, M. ; Martínez-Subiela, S. ; Tvarijonaviciute, A. ; Tecles, F.</creatorcontrib><description>The aim of this study was to validate automated methods to measure iron (Fe), zinc (Zn), copper (Cu) and ferritin in pig saliva samples. A complete analytical validation was performed of all assays. In addition, these methods were applied to saliva of Fe supplemented (n = 22) and non-supplemented (n = 20) piglets. All assays were able to measure these biomarkers in pig saliva with adequate precision, accuracy and high sensitivity and, in case of trace elements without needing a deproteinization pre-process. The group of piglets supplemented with Fe presented significantly higher levels of ferritin and Zn in saliva. In conclusion, the automated assays evaluated were able to measure Fe, Zn, Cu and ferritin in saliva of pigs, and in case of trace elements, they have the advantage of not needing a deproteinization pre-treatment and thus these analytes can be measured in a simple and fast manner.
•Iron (Fe), zinc (Zn) and copper (Cu) were analysed by automated assays in pig saliva.•Selected trace elements were measured reliably without pre-treatment in saliva.•Ferritin can be measured in pig saliva.•Ferritin and Zinc in saliva increased in pigs after iron supplementation.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2022.09.007</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Analytical chemistry ; Anemia ; Animals ; Assaying ; Automated assay ; Automation ; Biomarkers ; Blood ; chemical species ; Copper ; Deproteinization ; Evaluation ; Ferritin ; Hematology ; Hemoglobin ; Hogs ; Immune system ; Inflammation ; Iron ; Laboratories ; Methods ; Pig saliva ; Plasma ; Saliva ; Swine ; Trace element ; Trace elements ; Veterinary medicine ; Zinc</subject><ispartof>Research in veterinary science, 2022-12, Vol.152, p.410-416</ispartof><rights>2022 The Authors</rights><rights>2022. The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-aa92a9a2f3963adb91b973c442614fe66519ae974b71e127a02bd595212f2e083</citedby><cites>FETCH-LOGICAL-c368t-aa92a9a2f3963adb91b973c442614fe66519ae974b71e127a02bd595212f2e083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rvsc.2022.09.007$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,778,782,3539,27913,27914,45984</link.rule.ids></links><search><creatorcontrib>Ortín-Bustillo, A.</creatorcontrib><creatorcontrib>Vidal, G. Ramis</creatorcontrib><creatorcontrib>Tortosa, D. Escribano</creatorcontrib><creatorcontrib>López-Arjona, M.</creatorcontrib><creatorcontrib>Rubio, C. Peres</creatorcontrib><creatorcontrib>Cerón, J.J.</creatorcontrib><creatorcontrib>Muñoz-Prieto, A.</creatorcontrib><creatorcontrib>Pardo-Marín, L.</creatorcontrib><creatorcontrib>López-Martínez, M.J.</creatorcontrib><creatorcontrib>Botia, M.</creatorcontrib><creatorcontrib>Martínez-Subiela, S.</creatorcontrib><creatorcontrib>Tvarijonaviciute, A.</creatorcontrib><creatorcontrib>Tecles, F.</creatorcontrib><title>Automated assays for trace elements and ferritin measurement in saliva of pigs: Analytical validation and a pilot application to evaluate different iron status</title><title>Research in veterinary science</title><description>The aim of this study was to validate automated methods to measure iron (Fe), zinc (Zn), copper (Cu) and ferritin in pig saliva samples. A complete analytical validation was performed of all assays. In addition, these methods were applied to saliva of Fe supplemented (n = 22) and non-supplemented (n = 20) piglets. All assays were able to measure these biomarkers in pig saliva with adequate precision, accuracy and high sensitivity and, in case of trace elements without needing a deproteinization pre-process. The group of piglets supplemented with Fe presented significantly higher levels of ferritin and Zn in saliva. In conclusion, the automated assays evaluated were able to measure Fe, Zn, Cu and ferritin in saliva of pigs, and in case of trace elements, they have the advantage of not needing a deproteinization pre-treatment and thus these analytes can be measured in a simple and fast manner.
•Iron (Fe), zinc (Zn) and copper (Cu) were analysed by automated assays in pig saliva.•Selected trace elements were measured reliably without pre-treatment in saliva.•Ferritin can be measured in pig saliva.•Ferritin and Zinc in saliva increased in pigs after iron supplementation.</description><subject>Analytical chemistry</subject><subject>Anemia</subject><subject>Animals</subject><subject>Assaying</subject><subject>Automated assay</subject><subject>Automation</subject><subject>Biomarkers</subject><subject>Blood</subject><subject>chemical species</subject><subject>Copper</subject><subject>Deproteinization</subject><subject>Evaluation</subject><subject>Ferritin</subject><subject>Hematology</subject><subject>Hemoglobin</subject><subject>Hogs</subject><subject>Immune system</subject><subject>Inflammation</subject><subject>Iron</subject><subject>Laboratories</subject><subject>Methods</subject><subject>Pig saliva</subject><subject>Plasma</subject><subject>Saliva</subject><subject>Swine</subject><subject>Trace element</subject><subject>Trace elements</subject><subject>Veterinary medicine</subject><subject>Zinc</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqNkU-r1DAUxYsoOD79Aq4Cbty05k-bTMTN8Hj-gQdudB3upLeSoW1qbjown8avaqbjyoW4Csn5nXPDPVX1WvBGcKHfnZp0Jt9ILmXDbcO5eVLtRKdkLbUWT6sd56qtO7nfP69eEJ04560QZlf9Oqw5TpCxZ0AEF2JDTCwn8MhwxAnnTAzmng2YUshhZhMCrWlTWLkSjOEMLA5sCT_oPTvMMF5y8DCyc5F6yCHOWwIUYoyZwbKMRd_ec2RYsLV8gPVhKEO22FQkypBXelk9G2AkfPXnvKu-f3z4dv-5fvz66cv94bH2Su9zDWAlWJCDslpBf7TiaI3ybSu1aAfUuhMW0Jr2aAQKaYDLY9_ZTgo5SOR7dVe9veUuKf5ckbKbAnkcR5gxruSkkUpwYzv-H6jojFWmawv65i_0FNdUNrQFWm6U1ldK3iifIlHCwS0pTJAuTnB3rded3LVed63XcetKvcX04WbCspVzwOTIB5w99iGhz66P4V_23-4lsGM</recordid><startdate>20221220</startdate><enddate>20221220</enddate><creator>Ortín-Bustillo, A.</creator><creator>Vidal, G. Ramis</creator><creator>Tortosa, D. Escribano</creator><creator>López-Arjona, M.</creator><creator>Rubio, C. Peres</creator><creator>Cerón, J.J.</creator><creator>Muñoz-Prieto, A.</creator><creator>Pardo-Marín, L.</creator><creator>López-Martínez, M.J.</creator><creator>Botia, M.</creator><creator>Martínez-Subiela, S.</creator><creator>Tvarijonaviciute, A.</creator><creator>Tecles, F.</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20221220</creationdate><title>Automated assays for trace elements and ferritin measurement in saliva of pigs: Analytical validation and a pilot application to evaluate different iron status</title><author>Ortín-Bustillo, A. ; Vidal, G. Ramis ; Tortosa, D. Escribano ; López-Arjona, M. ; Rubio, C. Peres ; Cerón, J.J. ; Muñoz-Prieto, A. ; Pardo-Marín, L. ; López-Martínez, M.J. ; Botia, M. ; Martínez-Subiela, S. ; Tvarijonaviciute, A. ; Tecles, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-aa92a9a2f3963adb91b973c442614fe66519ae974b71e127a02bd595212f2e083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analytical chemistry</topic><topic>Anemia</topic><topic>Animals</topic><topic>Assaying</topic><topic>Automated assay</topic><topic>Automation</topic><topic>Biomarkers</topic><topic>Blood</topic><topic>chemical species</topic><topic>Copper</topic><topic>Deproteinization</topic><topic>Evaluation</topic><topic>Ferritin</topic><topic>Hematology</topic><topic>Hemoglobin</topic><topic>Hogs</topic><topic>Immune system</topic><topic>Inflammation</topic><topic>Iron</topic><topic>Laboratories</topic><topic>Methods</topic><topic>Pig saliva</topic><topic>Plasma</topic><topic>Saliva</topic><topic>Swine</topic><topic>Trace element</topic><topic>Trace elements</topic><topic>Veterinary medicine</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ortín-Bustillo, A.</creatorcontrib><creatorcontrib>Vidal, G. Ramis</creatorcontrib><creatorcontrib>Tortosa, D. Escribano</creatorcontrib><creatorcontrib>López-Arjona, M.</creatorcontrib><creatorcontrib>Rubio, C. Peres</creatorcontrib><creatorcontrib>Cerón, J.J.</creatorcontrib><creatorcontrib>Muñoz-Prieto, A.</creatorcontrib><creatorcontrib>Pardo-Marín, L.</creatorcontrib><creatorcontrib>López-Martínez, M.J.</creatorcontrib><creatorcontrib>Botia, M.</creatorcontrib><creatorcontrib>Martínez-Subiela, S.</creatorcontrib><creatorcontrib>Tvarijonaviciute, A.</creatorcontrib><creatorcontrib>Tecles, F.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ortín-Bustillo, A.</au><au>Vidal, G. Ramis</au><au>Tortosa, D. Escribano</au><au>López-Arjona, M.</au><au>Rubio, C. Peres</au><au>Cerón, J.J.</au><au>Muñoz-Prieto, A.</au><au>Pardo-Marín, L.</au><au>López-Martínez, M.J.</au><au>Botia, M.</au><au>Martínez-Subiela, S.</au><au>Tvarijonaviciute, A.</au><au>Tecles, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Automated assays for trace elements and ferritin measurement in saliva of pigs: Analytical validation and a pilot application to evaluate different iron status</atitle><jtitle>Research in veterinary science</jtitle><date>2022-12-20</date><risdate>2022</risdate><volume>152</volume><spage>410</spage><epage>416</epage><pages>410-416</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>The aim of this study was to validate automated methods to measure iron (Fe), zinc (Zn), copper (Cu) and ferritin in pig saliva samples. A complete analytical validation was performed of all assays. In addition, these methods were applied to saliva of Fe supplemented (n = 22) and non-supplemented (n = 20) piglets. All assays were able to measure these biomarkers in pig saliva with adequate precision, accuracy and high sensitivity and, in case of trace elements without needing a deproteinization pre-process. The group of piglets supplemented with Fe presented significantly higher levels of ferritin and Zn in saliva. In conclusion, the automated assays evaluated were able to measure Fe, Zn, Cu and ferritin in saliva of pigs, and in case of trace elements, they have the advantage of not needing a deproteinization pre-treatment and thus these analytes can be measured in a simple and fast manner.
•Iron (Fe), zinc (Zn) and copper (Cu) were analysed by automated assays in pig saliva.•Selected trace elements were measured reliably without pre-treatment in saliva.•Ferritin can be measured in pig saliva.•Ferritin and Zinc in saliva increased in pigs after iron supplementation.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.rvsc.2022.09.007</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0034-5288 |
| ispartof | Research in veterinary science, 2022-12, Vol.152, p.410-416 |
| issn | 0034-5288 1532-2661 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2723107950 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings |
| subjects | Analytical chemistry Anemia Animals Assaying Automated assay Automation Biomarkers Blood chemical species Copper Deproteinization Evaluation Ferritin Hematology Hemoglobin Hogs Immune system Inflammation Iron Laboratories Methods Pig saliva Plasma Saliva Swine Trace element Trace elements Veterinary medicine Zinc |
| title | Automated assays for trace elements and ferritin measurement in saliva of pigs: Analytical validation and a pilot application to evaluate different iron status |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T09%3A09%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Automated%20assays%20for%20trace%20elements%20and%20ferritin%20measurement%20in%20saliva%20of%20pigs:%20Analytical%20validation%20and%20a%20pilot%20application%20to%20evaluate%20different%20iron%20status&rft.jtitle=Research%20in%20veterinary%20science&rft.au=Ort%C3%ADn-Bustillo,%20A.&rft.date=2022-12-20&rft.volume=152&rft.spage=410&rft.epage=416&rft.pages=410-416&rft.issn=0034-5288&rft.eissn=1532-2661&rft_id=info:doi/10.1016/j.rvsc.2022.09.007&rft_dat=%3Cproquest_cross%3E2729073664%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2729073664&rft_id=info:pmid/&rft_els_id=S0034528822002880&rfr_iscdi=true |