Abrusamide H Impairs the Secretion of the Cytokines in RAW264.7 Cells and the Inflammatory Infiltration in Tail Transection‐Induced Zebrafish

Abrus mollis Hance (Leguminosae) has a variety of biological activities, including anti‐inflammatory, antioxidant, antibacterial, antiviral, and antitumor activities. However, the specific substances responsible for the anti‐inflammatory effects are unknown. Abrusamide H (BJBS) is a truxillic acid d...

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Veröffentlicht in:Chemistry & biodiversity 2022-11, Vol.19 (11), p.e202200474-n/a
Hauptverfasser: Liu, Roujia, Zhou, Feirong, Yu, Jiaxian, Wei, Xinru, Liu, Xiangying, Yuan, Xujiang, Yu, Chuqin
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container_issue 11
container_start_page e202200474
container_title Chemistry & biodiversity
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creator Liu, Roujia
Zhou, Feirong
Yu, Jiaxian
Wei, Xinru
Liu, Xiangying
Yuan, Xujiang
Yu, Chuqin
description Abrus mollis Hance (Leguminosae) has a variety of biological activities, including anti‐inflammatory, antioxidant, antibacterial, antiviral, and antitumor activities. However, the specific substances responsible for the anti‐inflammatory effects are unknown. Abrusamide H (BJBS) is a truxillic acid derivative obtained from the leaves of Abrus mollis Hance and has potential anti‐inflammatory effects. In this study, we aimed to estimate the potential effect and mechanism of BJBS in inflammation by establishing lipopolysaccharide (LPS)‐stimulated RAW264.7 cells in vitro and an injured zebrafish tail fin in vivo. The RAW264.7 cells were treated with different concentrations of BJBS after LPS stimulation. The production of nitric oxide (NO) was detected by Griess reaction, and reactive oxygen species (ROS) were detected by an ROS assay kit. The levels of proinflammatory cytokines, including interleukin 6 (IL‐6), tumor necrosis factor α (TNF‐α), interleukin 1β (IL‐1β), and interleukin 18 (IL‐18) were measured by ELISA. Results showed that BJBS at all concentrations inhibited the proliferation of RAW264.7 macrophages after LPS stimulation by cell counting kit‐8 and the production of NO and ROS. In the BJBS treatment group, the levels of IL‐6, TNF‐α, IL‐1β, and IL‐18 decreased in a concentration‐dependent manner. The results in vivo showed that no significant difference in the survival of zebrafish between the BJBS and blank groups and BJBS inhibited the migration and aggregation of zebrafish neutrophils in a dose‐dependent manner in inflammation induced by tail transection‐induced inflammation. In conclusion, BJBS inhibited the production of NO and ROS, decreased the levels of secreted IL‐6, TNF‐α, IL‐1β, and IL‐18, and reduced the migration and aggregation of zebrafish neutrophils.
doi_str_mv 10.1002/cbdv.202200474
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However, the specific substances responsible for the anti‐inflammatory effects are unknown. Abrusamide H (BJBS) is a truxillic acid derivative obtained from the leaves of Abrus mollis Hance and has potential anti‐inflammatory effects. In this study, we aimed to estimate the potential effect and mechanism of BJBS in inflammation by establishing lipopolysaccharide (LPS)‐stimulated RAW264.7 cells in vitro and an injured zebrafish tail fin in vivo. The RAW264.7 cells were treated with different concentrations of BJBS after LPS stimulation. The production of nitric oxide (NO) was detected by Griess reaction, and reactive oxygen species (ROS) were detected by an ROS assay kit. The levels of proinflammatory cytokines, including interleukin 6 (IL‐6), tumor necrosis factor α (TNF‐α), interleukin 1β (IL‐1β), and interleukin 18 (IL‐18) were measured by ELISA. Results showed that BJBS at all concentrations inhibited the proliferation of RAW264.7 macrophages after LPS stimulation by cell counting kit‐8 and the production of NO and ROS. In the BJBS treatment group, the levels of IL‐6, TNF‐α, IL‐1β, and IL‐18 decreased in a concentration‐dependent manner. The results in vivo showed that no significant difference in the survival of zebrafish between the BJBS and blank groups and BJBS inhibited the migration and aggregation of zebrafish neutrophils in a dose‐dependent manner in inflammation induced by tail transection‐induced inflammation. In conclusion, BJBS inhibited the production of NO and ROS, decreased the levels of secreted IL‐6, TNF‐α, IL‐1β, and IL‐18, and reduced the migration and aggregation of zebrafish neutrophils.</description><identifier>ISSN: 1612-1872</identifier><identifier>EISSN: 1612-1880</identifier><identifier>DOI: 10.1002/cbdv.202200474</identifier><language>eng</language><publisher>Weinheim: Wiley Subscription Services, Inc</publisher><subject>Abrus mollis ; abrusamide H ; Agglomeration ; Anticancer properties ; Cytokines ; Danio rerio ; Enzyme-linked immunosorbent assay ; Inflammation ; Interleukin 18 ; Interleukin 6 ; Leukocyte migration ; Leukocytes (neutrophilic) ; Lipopolysaccharides ; LPS ; Macrophages ; Metastases ; Neutrophils ; Nitric oxide ; RAW264.7 ; Reactive oxygen species ; Stimulation ; Tails ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Zebrafish</subject><ispartof>Chemistry &amp; biodiversity, 2022-11, Vol.19 (11), p.e202200474-n/a</ispartof><rights>2022 Wiley‐VHCA AG, Zurich, Switzerland</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3504-7c50282e53f3d3b431fb63c5cfe58a72d918237a93a9fe81439f5e71f3b291693</citedby><cites>FETCH-LOGICAL-c3504-7c50282e53f3d3b431fb63c5cfe58a72d918237a93a9fe81439f5e71f3b291693</cites><orcidid>0000-0002-2021-014X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbdv.202200474$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbdv.202200474$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Liu, Roujia</creatorcontrib><creatorcontrib>Zhou, Feirong</creatorcontrib><creatorcontrib>Yu, Jiaxian</creatorcontrib><creatorcontrib>Wei, Xinru</creatorcontrib><creatorcontrib>Liu, Xiangying</creatorcontrib><creatorcontrib>Yuan, Xujiang</creatorcontrib><creatorcontrib>Yu, Chuqin</creatorcontrib><title>Abrusamide H Impairs the Secretion of the Cytokines in RAW264.7 Cells and the Inflammatory Infiltration in Tail Transection‐Induced Zebrafish</title><title>Chemistry &amp; biodiversity</title><description>Abrus mollis Hance (Leguminosae) has a variety of biological activities, including anti‐inflammatory, antioxidant, antibacterial, antiviral, and antitumor activities. However, the specific substances responsible for the anti‐inflammatory effects are unknown. Abrusamide H (BJBS) is a truxillic acid derivative obtained from the leaves of Abrus mollis Hance and has potential anti‐inflammatory effects. In this study, we aimed to estimate the potential effect and mechanism of BJBS in inflammation by establishing lipopolysaccharide (LPS)‐stimulated RAW264.7 cells in vitro and an injured zebrafish tail fin in vivo. The RAW264.7 cells were treated with different concentrations of BJBS after LPS stimulation. The production of nitric oxide (NO) was detected by Griess reaction, and reactive oxygen species (ROS) were detected by an ROS assay kit. The levels of proinflammatory cytokines, including interleukin 6 (IL‐6), tumor necrosis factor α (TNF‐α), interleukin 1β (IL‐1β), and interleukin 18 (IL‐18) were measured by ELISA. Results showed that BJBS at all concentrations inhibited the proliferation of RAW264.7 macrophages after LPS stimulation by cell counting kit‐8 and the production of NO and ROS. In the BJBS treatment group, the levels of IL‐6, TNF‐α, IL‐1β, and IL‐18 decreased in a concentration‐dependent manner. The results in vivo showed that no significant difference in the survival of zebrafish between the BJBS and blank groups and BJBS inhibited the migration and aggregation of zebrafish neutrophils in a dose‐dependent manner in inflammation induced by tail transection‐induced inflammation. In conclusion, BJBS inhibited the production of NO and ROS, decreased the levels of secreted IL‐6, TNF‐α, IL‐1β, and IL‐18, and reduced the migration and aggregation of zebrafish neutrophils.</description><subject>Abrus mollis</subject><subject>abrusamide H</subject><subject>Agglomeration</subject><subject>Anticancer properties</subject><subject>Cytokines</subject><subject>Danio rerio</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Inflammation</subject><subject>Interleukin 18</subject><subject>Interleukin 6</subject><subject>Leukocyte migration</subject><subject>Leukocytes (neutrophilic)</subject><subject>Lipopolysaccharides</subject><subject>LPS</subject><subject>Macrophages</subject><subject>Metastases</subject><subject>Neutrophils</subject><subject>Nitric oxide</subject><subject>RAW264.7</subject><subject>Reactive oxygen species</subject><subject>Stimulation</subject><subject>Tails</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><subject>Zebrafish</subject><issn>1612-1872</issn><issn>1612-1880</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkc9q20AQxkVJoI7Ta88LveRiZ_9IWu3RVZrYYAikbgu9iNVqFq8rrZxdqcG3vEHyjHmSrOzgQC89zczH7xtm-KLoM8FTgjG9VGX1d0oxpRjHPP4QjUhK6IRkGT459px-jM683wQ-6NkoepqVrveyMRWgOVo0W2mcR90a0HdQDjrTWtTqvZDvuvaPseCRsehu9oum8ZSjHOraI2mrPbOwupZNI7vW7YbB1J2T-yXBs5KmRisnrQc1aC-Pzwtb9Qoq9BtKJ7Xx6_PoVMvaw6e3Oo5-XH9b5fPJ8vZmkc-WE8USHE-4SsIHFBKmWcXKmBFdpkwlSkOSSU4rQTLKuBRMCg0ZiZnQCXCiWUkFSQUbRxeHvVvX3vfgu6IxXoVfpIW29wXlFAsqYpYG9Ms_6KbtnQ3XBYrxJEtpOlDTA6Vc670DXWydaaTbFQQXQz7FkE9xzCcYxMHwYGrY_Ycu8q9XP9-9r2XXlLE</recordid><startdate>202211</startdate><enddate>202211</enddate><creator>Liu, Roujia</creator><creator>Zhou, Feirong</creator><creator>Yu, Jiaxian</creator><creator>Wei, Xinru</creator><creator>Liu, Xiangying</creator><creator>Yuan, Xujiang</creator><creator>Yu, Chuqin</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2021-014X</orcidid></search><sort><creationdate>202211</creationdate><title>Abrusamide H Impairs the Secretion of the Cytokines in RAW264.7 Cells and the Inflammatory Infiltration in Tail Transection‐Induced Zebrafish</title><author>Liu, Roujia ; Zhou, Feirong ; Yu, Jiaxian ; Wei, Xinru ; Liu, Xiangying ; Yuan, Xujiang ; Yu, Chuqin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3504-7c50282e53f3d3b431fb63c5cfe58a72d918237a93a9fe81439f5e71f3b291693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Abrus mollis</topic><topic>abrusamide H</topic><topic>Agglomeration</topic><topic>Anticancer properties</topic><topic>Cytokines</topic><topic>Danio rerio</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Inflammation</topic><topic>Interleukin 18</topic><topic>Interleukin 6</topic><topic>Leukocyte migration</topic><topic>Leukocytes (neutrophilic)</topic><topic>Lipopolysaccharides</topic><topic>LPS</topic><topic>Macrophages</topic><topic>Metastases</topic><topic>Neutrophils</topic><topic>Nitric oxide</topic><topic>RAW264.7</topic><topic>Reactive oxygen species</topic><topic>Stimulation</topic><topic>Tails</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Roujia</creatorcontrib><creatorcontrib>Zhou, Feirong</creatorcontrib><creatorcontrib>Yu, Jiaxian</creatorcontrib><creatorcontrib>Wei, Xinru</creatorcontrib><creatorcontrib>Liu, Xiangying</creatorcontrib><creatorcontrib>Yuan, Xujiang</creatorcontrib><creatorcontrib>Yu, Chuqin</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry &amp; biodiversity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Roujia</au><au>Zhou, Feirong</au><au>Yu, Jiaxian</au><au>Wei, Xinru</au><au>Liu, Xiangying</au><au>Yuan, Xujiang</au><au>Yu, Chuqin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abrusamide H Impairs the Secretion of the Cytokines in RAW264.7 Cells and the Inflammatory Infiltration in Tail Transection‐Induced Zebrafish</atitle><jtitle>Chemistry &amp; biodiversity</jtitle><date>2022-11</date><risdate>2022</risdate><volume>19</volume><issue>11</issue><spage>e202200474</spage><epage>n/a</epage><pages>e202200474-n/a</pages><issn>1612-1872</issn><eissn>1612-1880</eissn><abstract>Abrus mollis Hance (Leguminosae) has a variety of biological activities, including anti‐inflammatory, antioxidant, antibacterial, antiviral, and antitumor activities. However, the specific substances responsible for the anti‐inflammatory effects are unknown. Abrusamide H (BJBS) is a truxillic acid derivative obtained from the leaves of Abrus mollis Hance and has potential anti‐inflammatory effects. In this study, we aimed to estimate the potential effect and mechanism of BJBS in inflammation by establishing lipopolysaccharide (LPS)‐stimulated RAW264.7 cells in vitro and an injured zebrafish tail fin in vivo. The RAW264.7 cells were treated with different concentrations of BJBS after LPS stimulation. The production of nitric oxide (NO) was detected by Griess reaction, and reactive oxygen species (ROS) were detected by an ROS assay kit. The levels of proinflammatory cytokines, including interleukin 6 (IL‐6), tumor necrosis factor α (TNF‐α), interleukin 1β (IL‐1β), and interleukin 18 (IL‐18) were measured by ELISA. Results showed that BJBS at all concentrations inhibited the proliferation of RAW264.7 macrophages after LPS stimulation by cell counting kit‐8 and the production of NO and ROS. In the BJBS treatment group, the levels of IL‐6, TNF‐α, IL‐1β, and IL‐18 decreased in a concentration‐dependent manner. The results in vivo showed that no significant difference in the survival of zebrafish between the BJBS and blank groups and BJBS inhibited the migration and aggregation of zebrafish neutrophils in a dose‐dependent manner in inflammation induced by tail transection‐induced inflammation. In conclusion, BJBS inhibited the production of NO and ROS, decreased the levels of secreted IL‐6, TNF‐α, IL‐1β, and IL‐18, and reduced the migration and aggregation of zebrafish neutrophils.</abstract><cop>Weinheim</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/cbdv.202200474</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-2021-014X</orcidid></addata></record>
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subjects Abrus mollis
abrusamide H
Agglomeration
Anticancer properties
Cytokines
Danio rerio
Enzyme-linked immunosorbent assay
Inflammation
Interleukin 18
Interleukin 6
Leukocyte migration
Leukocytes (neutrophilic)
Lipopolysaccharides
LPS
Macrophages
Metastases
Neutrophils
Nitric oxide
RAW264.7
Reactive oxygen species
Stimulation
Tails
Tumor necrosis factor-TNF
Tumor necrosis factor-α
Zebrafish
title Abrusamide H Impairs the Secretion of the Cytokines in RAW264.7 Cells and the Inflammatory Infiltration in Tail Transection‐Induced Zebrafish
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