Neutralizing the adverse effects of cyclophosphamide on the mouse testis and sperm parameters through pentoxifylline: A molecular and stereological study
Cyclophosphamide (CP) is one of the chemotherapeutic drugs, which plays its role by interfering with all rapidly proliferating tissues like cancer and testis. The aim of this study was to investigate the protective effect of pentoxifylline (PTX) on the sperm parameters, spermatogenesis indices, bioc...
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Veröffentlicht in: | Andrologia 2022-11, Vol.54 (10), p.e14543-n/a |
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description | Cyclophosphamide (CP) is one of the chemotherapeutic drugs, which plays its role by interfering with all rapidly proliferating tissues like cancer and testis. The aim of this study was to investigate the protective effect of pentoxifylline (PTX) on the sperm parameters, spermatogenesis indices, biochemical alterations and gene expressions, in adult male mice treated with CP. A total of 24 male NMRI mice were divided into four groups: control, CP group (15 mg/kg weekly), PTX (100 mg/kg daily) and CP + PTX and treated for 35 days with the intraperitoneal injection. A significant decrease in the spermatogenesis indices, Leydig cells, sperm motility, viability, count, tail length and daily sperm production was found in the CP group compared to the control group. The results of this study indicate that PTX prevented these adverse effects of CP and decreased the number of apoptotic cells. Moreover, the CP group showed decreased levels of total antioxidant capacity, testosterone, lipid peroxidation and the expression of cytochrome P450 and 3β‐hydroxysteroid, all of which were neutralized in the CP + PTX group. It seems that PTX has the potential to be used in therapeutic regimens of cancer patients to reduce the side effects of CP. However, more research is needed to evaluate this prevention in mice models of cancer. |
doi_str_mv | 10.1111/and.14543 |
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The aim of this study was to investigate the protective effect of pentoxifylline (PTX) on the sperm parameters, spermatogenesis indices, biochemical alterations and gene expressions, in adult male mice treated with CP. A total of 24 male NMRI mice were divided into four groups: control, CP group (15 mg/kg weekly), PTX (100 mg/kg daily) and CP + PTX and treated for 35 days with the intraperitoneal injection. A significant decrease in the spermatogenesis indices, Leydig cells, sperm motility, viability, count, tail length and daily sperm production was found in the CP group compared to the control group. The results of this study indicate that PTX prevented these adverse effects of CP and decreased the number of apoptotic cells. Moreover, the CP group showed decreased levels of total antioxidant capacity, testosterone, lipid peroxidation and the expression of cytochrome P450 and 3β‐hydroxysteroid, all of which were neutralized in the CP + PTX group. It seems that PTX has the potential to be used in therapeutic regimens of cancer patients to reduce the side effects of CP. However, more research is needed to evaluate this prevention in mice models of cancer.</description><identifier>ISSN: 0303-4569</identifier><identifier>EISSN: 1439-0272</identifier><identifier>DOI: 10.1111/and.14543</identifier><language>eng</language><publisher>Berlin: Wiley Subscription Services, Inc</publisher><subject>Animal models ; Apoptosis ; Cancer ; Cyclophosphamide ; Cytochrome P450 ; infertility ; Leydig cells ; Lipid peroxidation ; pentoxifylline ; Side effects ; Sperm ; Spermatogenesis ; testis ; Testosterone</subject><ispartof>Andrologia, 2022-11, Vol.54 (10), p.e14543-n/a</ispartof><rights>2022 Wiley‐VCH GmbH.</rights><rights>2022 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2533-b63d1ebd4d0bf3b5206b4b05cfe4c99a834e08b0ef19e1fecc3838f4e93c71823</cites><orcidid>0000-0003-0190-897X ; 0000-0001-8383-6150 ; 0000-0002-2400-313X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fand.14543$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fand.14543$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids></links><search><creatorcontrib>Mahmoodi, Monireh</creatorcontrib><creatorcontrib>Bakhshi, Sepideh</creatorcontrib><creatorcontrib>Sadeghzadeh, Firouzeh</creatorcontrib><title>Neutralizing the adverse effects of cyclophosphamide on the mouse testis and sperm parameters through pentoxifylline: A molecular and stereological study</title><title>Andrologia</title><description>Cyclophosphamide (CP) is one of the chemotherapeutic drugs, which plays its role by interfering with all rapidly proliferating tissues like cancer and testis. The aim of this study was to investigate the protective effect of pentoxifylline (PTX) on the sperm parameters, spermatogenesis indices, biochemical alterations and gene expressions, in adult male mice treated with CP. A total of 24 male NMRI mice were divided into four groups: control, CP group (15 mg/kg weekly), PTX (100 mg/kg daily) and CP + PTX and treated for 35 days with the intraperitoneal injection. A significant decrease in the spermatogenesis indices, Leydig cells, sperm motility, viability, count, tail length and daily sperm production was found in the CP group compared to the control group. The results of this study indicate that PTX prevented these adverse effects of CP and decreased the number of apoptotic cells. Moreover, the CP group showed decreased levels of total antioxidant capacity, testosterone, lipid peroxidation and the expression of cytochrome P450 and 3β‐hydroxysteroid, all of which were neutralized in the CP + PTX group. It seems that PTX has the potential to be used in therapeutic regimens of cancer patients to reduce the side effects of CP. However, more research is needed to evaluate this prevention in mice models of cancer.</description><subject>Animal models</subject><subject>Apoptosis</subject><subject>Cancer</subject><subject>Cyclophosphamide</subject><subject>Cytochrome P450</subject><subject>infertility</subject><subject>Leydig cells</subject><subject>Lipid peroxidation</subject><subject>pentoxifylline</subject><subject>Side effects</subject><subject>Sperm</subject><subject>Spermatogenesis</subject><subject>testis</subject><subject>Testosterone</subject><issn>0303-4569</issn><issn>1439-0272</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp10c1OxCAQB3BiNHGjHnwDEi966AqF7rbeNn4nRi96bigddjG0VGjV-ia-raP1ZOJcCMlvBv5AyCFnc451qtp6zmUmxRaZcSmKhKXLdJvMmGAikdmi2CUHMT4zLJktl1LOyOc9DH1Qzn7Ydk37DVBVv0KIQMEY0H2k3lA9aue7jY_dRjW2BurbH9r4AWEPsbeR4uE0dhAa2qmgGuhxCqrgh_WGdtD2_t2a0TnbwhldYa8DPTgVpkbU4J1fW60c7oZ63Cc7RrkIB7_rHnm6unw8v0nuHq5vz1d3iU4zIZJqIWoOVS1rVhlRZSlbVLJimTYgdVGoXEhgecXA8AI4RtIiF7mRUAi95Hkq9sjxNLcL_mXALGVjowbnVAuYr8QnZFIIWXCkR3_osx9Ci7f7ViLlaZrlqE4mpYOPMYApu2AbFcaSs_L7n0pMXP78E9rTyb5ZB-P_sFzdX0wdXwQpl9I</recordid><startdate>202211</startdate><enddate>202211</enddate><creator>Mahmoodi, Monireh</creator><creator>Bakhshi, Sepideh</creator><creator>Sadeghzadeh, Firouzeh</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0190-897X</orcidid><orcidid>https://orcid.org/0000-0001-8383-6150</orcidid><orcidid>https://orcid.org/0000-0002-2400-313X</orcidid></search><sort><creationdate>202211</creationdate><title>Neutralizing the adverse effects of cyclophosphamide on the mouse testis and sperm parameters through pentoxifylline: A molecular and stereological study</title><author>Mahmoodi, Monireh ; Bakhshi, Sepideh ; Sadeghzadeh, Firouzeh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2533-b63d1ebd4d0bf3b5206b4b05cfe4c99a834e08b0ef19e1fecc3838f4e93c71823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal models</topic><topic>Apoptosis</topic><topic>Cancer</topic><topic>Cyclophosphamide</topic><topic>Cytochrome P450</topic><topic>infertility</topic><topic>Leydig cells</topic><topic>Lipid peroxidation</topic><topic>pentoxifylline</topic><topic>Side effects</topic><topic>Sperm</topic><topic>Spermatogenesis</topic><topic>testis</topic><topic>Testosterone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mahmoodi, Monireh</creatorcontrib><creatorcontrib>Bakhshi, Sepideh</creatorcontrib><creatorcontrib>Sadeghzadeh, Firouzeh</creatorcontrib><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Andrologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mahmoodi, Monireh</au><au>Bakhshi, Sepideh</au><au>Sadeghzadeh, Firouzeh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutralizing the adverse effects of cyclophosphamide on the mouse testis and sperm parameters through pentoxifylline: A molecular and stereological study</atitle><jtitle>Andrologia</jtitle><date>2022-11</date><risdate>2022</risdate><volume>54</volume><issue>10</issue><spage>e14543</spage><epage>n/a</epage><pages>e14543-n/a</pages><issn>0303-4569</issn><eissn>1439-0272</eissn><abstract>Cyclophosphamide (CP) is one of the chemotherapeutic drugs, which plays its role by interfering with all rapidly proliferating tissues like cancer and testis. The aim of this study was to investigate the protective effect of pentoxifylline (PTX) on the sperm parameters, spermatogenesis indices, biochemical alterations and gene expressions, in adult male mice treated with CP. A total of 24 male NMRI mice were divided into four groups: control, CP group (15 mg/kg weekly), PTX (100 mg/kg daily) and CP + PTX and treated for 35 days with the intraperitoneal injection. A significant decrease in the spermatogenesis indices, Leydig cells, sperm motility, viability, count, tail length and daily sperm production was found in the CP group compared to the control group. The results of this study indicate that PTX prevented these adverse effects of CP and decreased the number of apoptotic cells. Moreover, the CP group showed decreased levels of total antioxidant capacity, testosterone, lipid peroxidation and the expression of cytochrome P450 and 3β‐hydroxysteroid, all of which were neutralized in the CP + PTX group. It seems that PTX has the potential to be used in therapeutic regimens of cancer patients to reduce the side effects of CP. However, more research is needed to evaluate this prevention in mice models of cancer.</abstract><cop>Berlin</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/and.14543</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-0190-897X</orcidid><orcidid>https://orcid.org/0000-0001-8383-6150</orcidid><orcidid>https://orcid.org/0000-0002-2400-313X</orcidid></addata></record> |
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subjects | Animal models Apoptosis Cancer Cyclophosphamide Cytochrome P450 infertility Leydig cells Lipid peroxidation pentoxifylline Side effects Sperm Spermatogenesis testis Testosterone |
title | Neutralizing the adverse effects of cyclophosphamide on the mouse testis and sperm parameters through pentoxifylline: A molecular and stereological study |
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