Anti‐Ro/SSA and/or anti‐La/SSB antibodies are associated with adverse endometrial status
Problem Anti‐Ro/SSA and/or anti‐La/SSB (anti‐SSA/SSB) antibodies impair pregnancy outcomes, including embryo implantation and pregnancy maintenance. Optimal endometrial immune status is essential for successful pregnancy. However, whether these antibodies affect endometrial immune status is still un...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 2023-06, Vol.89 (6), p.e13630-n/a |
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| container_title | American journal of reproductive immunology (1989) |
| container_volume | 89 |
| creator | Lu, Fangting Wang, Yanshi Fang, Xuhui Jin, Rentao Xu, Bo Qiu, Qiannan Wu, Li |
| description | Problem
Anti‐Ro/SSA and/or anti‐La/SSB (anti‐SSA/SSB) antibodies impair pregnancy outcomes, including embryo implantation and pregnancy maintenance. Optimal endometrial immune status is essential for successful pregnancy. However, whether these antibodies affect endometrial immune status is still unclear. Menstrual blood can be collected non‐invasively, differs from peripheral blood, and can reflect the endometrial immune status. We herein focused on changes in subsets of natural killer (NK) cells and T cells in menstrual blood.
Methods of study
Menstrual blood samples from anti‐SSA/SSB antibody–positive (n = 18) and anti‐SSA/SSB antibody–negative control (n = 8) women were collected, and the profile of lymphocyte subsets was analyzed. The phenotypes of menstrual blood CD49a− and CD49a+ NK cells were compared, and the abundance of NK and CD49a+ NK cells in menstrual blood of the two groups was assessed. Additionally, CD4+T and CD8+T cells were investigated for their ability to secret functional cytokines.
Results
Menstrual blood contains a large number of (mostly CD49a+) NK cells, which exhibited a more exhausted phenotype with greater expression of the immune checkpoint molecules programmed cell death protein 1 and Tim‐3 compared to CD49a− conventional NK cells. CD8+T cells in menstrual blood from anti‐SSA/SSB antibody–positive women produced a stronger response after stimulation, accompanied by increased interferon‐γ, tumor necrosis factor–α, and granzyme B secretion (P < 0.05, separately).
Conclusion
Menstrual blood cell composition differs between anti‐SSA/SSB antibody–positive women and normal controls, especially in terms of CD49a+ NK cells and CD8+T cells, unbalancing the immune cell composition and inflammatory uterine microenvironment and possibly contributing to adverse pregnancy outcomes. |
| doi_str_mv | 10.1111/aji.13630 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2720430213</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2814274525</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3530-c2e679b16ffbda45067d8a9d9887322b5534a5bb3e85d0e426bab0754bd41dd23</originalsourceid><addsrcrecordid>eNp1kM1OGzEUha2qiABl0ReoRuqmLCbxv51lQEBBkZCg3VWy7PGN6mgyTu2ZInY8Qp-RJ8EkoQsk7ubce_Tp6Oog9JngMSkzscswJkwy_AEdEIlxjfVUfSw75rJWHOsROsx5iXHxmdpHIyaJJlLqA_Rr1vXh6fHfbZzc3c0q2_lJTEU25twW83RzuegD5MomqGzOsQm2B1_dh_53Zf1fSBkq6HxcQZ-Cbavc237In9DewrYZjnd6hH5enP84-17Pby6vzmbzumGC4bqhINXUEblYOG-5wFJ5bad-qrVilDohGLfCOQZaeAycSmcdVoI7z4n3lB2hb9vcdYp_Bsi9WYXcQNvaDuKQDVUUc4YpYQX9-gZdxiF15TtDNeFUcUFFoU62VJNizgkWZp3CyqYHQ7B5qdyUys2m8sJ-2SUObgX-P_nacQEmW-A-tPDwfpKZXV9tI58BiqKK9Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2814274525</pqid></control><display><type>article</type><title>Anti‐Ro/SSA and/or anti‐La/SSB antibodies are associated with adverse endometrial status</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Lu, Fangting ; Wang, Yanshi ; Fang, Xuhui ; Jin, Rentao ; Xu, Bo ; Qiu, Qiannan ; Wu, Li</creator><creatorcontrib>Lu, Fangting ; Wang, Yanshi ; Fang, Xuhui ; Jin, Rentao ; Xu, Bo ; Qiu, Qiannan ; Wu, Li</creatorcontrib><description>Problem
Anti‐Ro/SSA and/or anti‐La/SSB (anti‐SSA/SSB) antibodies impair pregnancy outcomes, including embryo implantation and pregnancy maintenance. Optimal endometrial immune status is essential for successful pregnancy. However, whether these antibodies affect endometrial immune status is still unclear. Menstrual blood can be collected non‐invasively, differs from peripheral blood, and can reflect the endometrial immune status. We herein focused on changes in subsets of natural killer (NK) cells and T cells in menstrual blood.
Methods of study
Menstrual blood samples from anti‐SSA/SSB antibody–positive (n = 18) and anti‐SSA/SSB antibody–negative control (n = 8) women were collected, and the profile of lymphocyte subsets was analyzed. The phenotypes of menstrual blood CD49a− and CD49a+ NK cells were compared, and the abundance of NK and CD49a+ NK cells in menstrual blood of the two groups was assessed. Additionally, CD4+T and CD8+T cells were investigated for their ability to secret functional cytokines.
Results
Menstrual blood contains a large number of (mostly CD49a+) NK cells, which exhibited a more exhausted phenotype with greater expression of the immune checkpoint molecules programmed cell death protein 1 and Tim‐3 compared to CD49a− conventional NK cells. CD8+T cells in menstrual blood from anti‐SSA/SSB antibody–positive women produced a stronger response after stimulation, accompanied by increased interferon‐γ, tumor necrosis factor–α, and granzyme B secretion (P < 0.05, separately).
Conclusion
Menstrual blood cell composition differs between anti‐SSA/SSB antibody–positive women and normal controls, especially in terms of CD49a+ NK cells and CD8+T cells, unbalancing the immune cell composition and inflammatory uterine microenvironment and possibly contributing to adverse pregnancy outcomes.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/aji.13630</identifier><identifier>PMID: 36181668</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>adverse pregnancy outcomes ; Antibodies ; Antibodies, Antinuclear - metabolism ; anti‐SSA/SSB antibodies ; Apoptosis ; Blood ; CD4 antigen ; CD49a+ NK cells ; CD8 antigen ; CD8+T cells ; Cell death ; Endometrium ; Endometrium - metabolism ; Female ; Granzyme B ; Humans ; Immune checkpoint ; Immune status ; Implantation ; Inflammation ; Integrin alpha1 ; Killer Cells, Natural - metabolism ; Lymphocytes ; Lymphocytes T ; menstrual blood ; Menstruation ; Microenvironments ; Natural killer cells ; PD-1 protein ; Peripheral blood ; Phenotypes ; Pregnancy ; Pregnancy Outcome ; Ro(SSA) antigen</subject><ispartof>American journal of reproductive immunology (1989), 2023-06, Vol.89 (6), p.e13630-n/a</ispartof><rights>2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3530-c2e679b16ffbda45067d8a9d9887322b5534a5bb3e85d0e426bab0754bd41dd23</citedby><cites>FETCH-LOGICAL-c3530-c2e679b16ffbda45067d8a9d9887322b5534a5bb3e85d0e426bab0754bd41dd23</cites><orcidid>0000-0001-8681-597X ; 0000-0002-2842-2219</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faji.13630$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faji.13630$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36181668$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Fangting</creatorcontrib><creatorcontrib>Wang, Yanshi</creatorcontrib><creatorcontrib>Fang, Xuhui</creatorcontrib><creatorcontrib>Jin, Rentao</creatorcontrib><creatorcontrib>Xu, Bo</creatorcontrib><creatorcontrib>Qiu, Qiannan</creatorcontrib><creatorcontrib>Wu, Li</creatorcontrib><title>Anti‐Ro/SSA and/or anti‐La/SSB antibodies are associated with adverse endometrial status</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Problem
Anti‐Ro/SSA and/or anti‐La/SSB (anti‐SSA/SSB) antibodies impair pregnancy outcomes, including embryo implantation and pregnancy maintenance. Optimal endometrial immune status is essential for successful pregnancy. However, whether these antibodies affect endometrial immune status is still unclear. Menstrual blood can be collected non‐invasively, differs from peripheral blood, and can reflect the endometrial immune status. We herein focused on changes in subsets of natural killer (NK) cells and T cells in menstrual blood.
Methods of study
Menstrual blood samples from anti‐SSA/SSB antibody–positive (n = 18) and anti‐SSA/SSB antibody–negative control (n = 8) women were collected, and the profile of lymphocyte subsets was analyzed. The phenotypes of menstrual blood CD49a− and CD49a+ NK cells were compared, and the abundance of NK and CD49a+ NK cells in menstrual blood of the two groups was assessed. Additionally, CD4+T and CD8+T cells were investigated for their ability to secret functional cytokines.
Results
Menstrual blood contains a large number of (mostly CD49a+) NK cells, which exhibited a more exhausted phenotype with greater expression of the immune checkpoint molecules programmed cell death protein 1 and Tim‐3 compared to CD49a− conventional NK cells. CD8+T cells in menstrual blood from anti‐SSA/SSB antibody–positive women produced a stronger response after stimulation, accompanied by increased interferon‐γ, tumor necrosis factor–α, and granzyme B secretion (P < 0.05, separately).
Conclusion
Menstrual blood cell composition differs between anti‐SSA/SSB antibody–positive women and normal controls, especially in terms of CD49a+ NK cells and CD8+T cells, unbalancing the immune cell composition and inflammatory uterine microenvironment and possibly contributing to adverse pregnancy outcomes.</description><subject>adverse pregnancy outcomes</subject><subject>Antibodies</subject><subject>Antibodies, Antinuclear - metabolism</subject><subject>anti‐SSA/SSB antibodies</subject><subject>Apoptosis</subject><subject>Blood</subject><subject>CD4 antigen</subject><subject>CD49a+ NK cells</subject><subject>CD8 antigen</subject><subject>CD8+T cells</subject><subject>Cell death</subject><subject>Endometrium</subject><subject>Endometrium - metabolism</subject><subject>Female</subject><subject>Granzyme B</subject><subject>Humans</subject><subject>Immune checkpoint</subject><subject>Immune status</subject><subject>Implantation</subject><subject>Inflammation</subject><subject>Integrin alpha1</subject><subject>Killer Cells, Natural - metabolism</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>menstrual blood</subject><subject>Menstruation</subject><subject>Microenvironments</subject><subject>Natural killer cells</subject><subject>PD-1 protein</subject><subject>Peripheral blood</subject><subject>Phenotypes</subject><subject>Pregnancy</subject><subject>Pregnancy Outcome</subject><subject>Ro(SSA) antigen</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1OGzEUha2qiABl0ReoRuqmLCbxv51lQEBBkZCg3VWy7PGN6mgyTu2ZInY8Qp-RJ8EkoQsk7ubce_Tp6Oog9JngMSkzscswJkwy_AEdEIlxjfVUfSw75rJWHOsROsx5iXHxmdpHIyaJJlLqA_Rr1vXh6fHfbZzc3c0q2_lJTEU25twW83RzuegD5MomqGzOsQm2B1_dh_53Zf1fSBkq6HxcQZ-Cbavc237In9DewrYZjnd6hH5enP84-17Pby6vzmbzumGC4bqhINXUEblYOG-5wFJ5bad-qrVilDohGLfCOQZaeAycSmcdVoI7z4n3lB2hb9vcdYp_Bsi9WYXcQNvaDuKQDVUUc4YpYQX9-gZdxiF15TtDNeFUcUFFoU62VJNizgkWZp3CyqYHQ7B5qdyUys2m8sJ-2SUObgX-P_nacQEmW-A-tPDwfpKZXV9tI58BiqKK9Q</recordid><startdate>202306</startdate><enddate>202306</enddate><creator>Lu, Fangting</creator><creator>Wang, Yanshi</creator><creator>Fang, Xuhui</creator><creator>Jin, Rentao</creator><creator>Xu, Bo</creator><creator>Qiu, Qiannan</creator><creator>Wu, Li</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8681-597X</orcidid><orcidid>https://orcid.org/0000-0002-2842-2219</orcidid></search><sort><creationdate>202306</creationdate><title>Anti‐Ro/SSA and/or anti‐La/SSB antibodies are associated with adverse endometrial status</title><author>Lu, Fangting ; Wang, Yanshi ; Fang, Xuhui ; Jin, Rentao ; Xu, Bo ; Qiu, Qiannan ; Wu, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3530-c2e679b16ffbda45067d8a9d9887322b5534a5bb3e85d0e426bab0754bd41dd23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>adverse pregnancy outcomes</topic><topic>Antibodies</topic><topic>Antibodies, Antinuclear - metabolism</topic><topic>anti‐SSA/SSB antibodies</topic><topic>Apoptosis</topic><topic>Blood</topic><topic>CD4 antigen</topic><topic>CD49a+ NK cells</topic><topic>CD8 antigen</topic><topic>CD8+T cells</topic><topic>Cell death</topic><topic>Endometrium</topic><topic>Endometrium - metabolism</topic><topic>Female</topic><topic>Granzyme B</topic><topic>Humans</topic><topic>Immune checkpoint</topic><topic>Immune status</topic><topic>Implantation</topic><topic>Inflammation</topic><topic>Integrin alpha1</topic><topic>Killer Cells, Natural - metabolism</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>menstrual blood</topic><topic>Menstruation</topic><topic>Microenvironments</topic><topic>Natural killer cells</topic><topic>PD-1 protein</topic><topic>Peripheral blood</topic><topic>Phenotypes</topic><topic>Pregnancy</topic><topic>Pregnancy Outcome</topic><topic>Ro(SSA) antigen</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Fangting</creatorcontrib><creatorcontrib>Wang, Yanshi</creatorcontrib><creatorcontrib>Fang, Xuhui</creatorcontrib><creatorcontrib>Jin, Rentao</creatorcontrib><creatorcontrib>Xu, Bo</creatorcontrib><creatorcontrib>Qiu, Qiannan</creatorcontrib><creatorcontrib>Wu, Li</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Fangting</au><au>Wang, Yanshi</au><au>Fang, Xuhui</au><au>Jin, Rentao</au><au>Xu, Bo</au><au>Qiu, Qiannan</au><au>Wu, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti‐Ro/SSA and/or anti‐La/SSB antibodies are associated with adverse endometrial status</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2023-06</date><risdate>2023</risdate><volume>89</volume><issue>6</issue><spage>e13630</spage><epage>n/a</epage><pages>e13630-n/a</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>Problem
Anti‐Ro/SSA and/or anti‐La/SSB (anti‐SSA/SSB) antibodies impair pregnancy outcomes, including embryo implantation and pregnancy maintenance. Optimal endometrial immune status is essential for successful pregnancy. However, whether these antibodies affect endometrial immune status is still unclear. Menstrual blood can be collected non‐invasively, differs from peripheral blood, and can reflect the endometrial immune status. We herein focused on changes in subsets of natural killer (NK) cells and T cells in menstrual blood.
Methods of study
Menstrual blood samples from anti‐SSA/SSB antibody–positive (n = 18) and anti‐SSA/SSB antibody–negative control (n = 8) women were collected, and the profile of lymphocyte subsets was analyzed. The phenotypes of menstrual blood CD49a− and CD49a+ NK cells were compared, and the abundance of NK and CD49a+ NK cells in menstrual blood of the two groups was assessed. Additionally, CD4+T and CD8+T cells were investigated for their ability to secret functional cytokines.
Results
Menstrual blood contains a large number of (mostly CD49a+) NK cells, which exhibited a more exhausted phenotype with greater expression of the immune checkpoint molecules programmed cell death protein 1 and Tim‐3 compared to CD49a− conventional NK cells. CD8+T cells in menstrual blood from anti‐SSA/SSB antibody–positive women produced a stronger response after stimulation, accompanied by increased interferon‐γ, tumor necrosis factor–α, and granzyme B secretion (P < 0.05, separately).
Conclusion
Menstrual blood cell composition differs between anti‐SSA/SSB antibody–positive women and normal controls, especially in terms of CD49a+ NK cells and CD8+T cells, unbalancing the immune cell composition and inflammatory uterine microenvironment and possibly contributing to adverse pregnancy outcomes.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36181668</pmid><doi>10.1111/aji.13630</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-8681-597X</orcidid><orcidid>https://orcid.org/0000-0002-2842-2219</orcidid></addata></record> |
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| ispartof | American journal of reproductive immunology (1989), 2023-06, Vol.89 (6), p.e13630-n/a |
| issn | 1046-7408 1600-0897 |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | adverse pregnancy outcomes Antibodies Antibodies, Antinuclear - metabolism anti‐SSA/SSB antibodies Apoptosis Blood CD4 antigen CD49a+ NK cells CD8 antigen CD8+T cells Cell death Endometrium Endometrium - metabolism Female Granzyme B Humans Immune checkpoint Immune status Implantation Inflammation Integrin alpha1 Killer Cells, Natural - metabolism Lymphocytes Lymphocytes T menstrual blood Menstruation Microenvironments Natural killer cells PD-1 protein Peripheral blood Phenotypes Pregnancy Pregnancy Outcome Ro(SSA) antigen |
| title | Anti‐Ro/SSA and/or anti‐La/SSB antibodies are associated with adverse endometrial status |
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