Analysis of MSCs' secretome and EVs cargo: Evaluation of functions and applications
Mesenchymal stem cells (MSCs) can exert different functions and can be used in several medical fields. In the last years, MSC properties have been attributed to their secreted factors such as soluble proteins, cytokines and growth factors. Moreover, a key role is played by the extracellular vesicles...
Gespeichert in:
| Veröffentlicht in: | Life sciences (1973) 2022-11, Vol.308, p.120990-120990, Article 120990 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 120990 |
|---|---|
| container_issue | |
| container_start_page | 120990 |
| container_title | Life sciences (1973) |
| container_volume | 308 |
| creator | Urrata, Valentina Trapani, Marco Franza, Mara Moschella, Francesco Di Stefano, Anna Barbara Toia, Francesca |
| description | Mesenchymal stem cells (MSCs) can exert different functions and can be used in several medical fields. In the last years, MSC properties have been attributed to their secreted factors such as soluble proteins, cytokines and growth factors. Moreover, a key role is played by the extracellular vesicles (EVs) which lead a heterogeneous cargo of proteins, lipids and small and long non-coding RNAs that interfere with the pathways of the recipient cells. Due to the safeness and easiness in obtaining the secretome, its use is becoming a turning point for the application in physiological and pathological fields. This review summarizes the most recent studies on the use of MSCs secretome, focusing on some physiological (angiogenesis and osteogenesis) and pathological (cancer, cardiovascular and autoimmune diseases) applications. The secreted analyzed factors are listed in a table. In addition, the different characteristics of the fetal MSCs derived secretome and the differences in the secretome composition of three-dimensionally (3D) cultured cells are discussed. As very innovative aspects, recent studies on some applications of engineered vesicles, embedded or not in three-dimensional structures, are treated and the influence of some epigenetic modifications on cells and EVs is investigated.
[Display omitted] |
| doi_str_mv | 10.1016/j.lfs.2022.120990 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2718632652</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0024320522006907</els_id><sourcerecordid>2718632652</sourcerecordid><originalsourceid>FETCH-LOGICAL-c212t-38e48ca7f348fc3e532ee4fa34a61c1219acd2e5ef6be8ad4ceebab9ff44880e3</originalsourceid><addsrcrecordid>eNp9kMtOwzAQRS0EEqXwAeyyg02CX3nBqqrKQypiUWBruc4YuUrj4Ekq9e9JCGtWc6U5d6Q5hFwzmjDKsrtdUltMOOU8YZyWJT0hM1bkZUwzwU7JjFIuY8Fpek4uEHeU0jTNxYxsFo2uj-gw8jZ63SzxJkIwATq_h0g3VbT6xMjo8OXvo9VB173unG9G2PaNGTP-Yrpta2d-l3hJzqyuEa7-5px8PK7el8_x-u3pZblYx4Yz3sWiAFkYnVshC2sEpIIDSKuF1BkzjLNSm4pDCjbbQqEraQC2eltaK2VRUBBzcjvdbYP_7gE7tXdooK51A75HxXNWZIJnKR9QNqEmeMQAVrXB7XU4KkbVKFDt1CBQjQLVJHDoPEwdGH44OAgKjYPGQOUCmE5V3v3T_gFW73lz</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2718632652</pqid></control><display><type>article</type><title>Analysis of MSCs' secretome and EVs cargo: Evaluation of functions and applications</title><source>Elsevier ScienceDirect Journals</source><creator>Urrata, Valentina ; Trapani, Marco ; Franza, Mara ; Moschella, Francesco ; Di Stefano, Anna Barbara ; Toia, Francesca</creator><creatorcontrib>Urrata, Valentina ; Trapani, Marco ; Franza, Mara ; Moschella, Francesco ; Di Stefano, Anna Barbara ; Toia, Francesca</creatorcontrib><description>Mesenchymal stem cells (MSCs) can exert different functions and can be used in several medical fields. In the last years, MSC properties have been attributed to their secreted factors such as soluble proteins, cytokines and growth factors. Moreover, a key role is played by the extracellular vesicles (EVs) which lead a heterogeneous cargo of proteins, lipids and small and long non-coding RNAs that interfere with the pathways of the recipient cells. Due to the safeness and easiness in obtaining the secretome, its use is becoming a turning point for the application in physiological and pathological fields. This review summarizes the most recent studies on the use of MSCs secretome, focusing on some physiological (angiogenesis and osteogenesis) and pathological (cancer, cardiovascular and autoimmune diseases) applications. The secreted analyzed factors are listed in a table. In addition, the different characteristics of the fetal MSCs derived secretome and the differences in the secretome composition of three-dimensionally (3D) cultured cells are discussed. As very innovative aspects, recent studies on some applications of engineered vesicles, embedded or not in three-dimensional structures, are treated and the influence of some epigenetic modifications on cells and EVs is investigated.
[Display omitted]</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2022.120990</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>3D cultures ; Epigenetic ; Extracellular vesicles ; Mesenchymal stem cells ; miRNAs ; Secretome</subject><ispartof>Life sciences (1973), 2022-11, Vol.308, p.120990-120990, Article 120990</ispartof><rights>2022 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c212t-38e48ca7f348fc3e532ee4fa34a61c1219acd2e5ef6be8ad4ceebab9ff44880e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0024320522006907$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Urrata, Valentina</creatorcontrib><creatorcontrib>Trapani, Marco</creatorcontrib><creatorcontrib>Franza, Mara</creatorcontrib><creatorcontrib>Moschella, Francesco</creatorcontrib><creatorcontrib>Di Stefano, Anna Barbara</creatorcontrib><creatorcontrib>Toia, Francesca</creatorcontrib><title>Analysis of MSCs' secretome and EVs cargo: Evaluation of functions and applications</title><title>Life sciences (1973)</title><description>Mesenchymal stem cells (MSCs) can exert different functions and can be used in several medical fields. In the last years, MSC properties have been attributed to their secreted factors such as soluble proteins, cytokines and growth factors. Moreover, a key role is played by the extracellular vesicles (EVs) which lead a heterogeneous cargo of proteins, lipids and small and long non-coding RNAs that interfere with the pathways of the recipient cells. Due to the safeness and easiness in obtaining the secretome, its use is becoming a turning point for the application in physiological and pathological fields. This review summarizes the most recent studies on the use of MSCs secretome, focusing on some physiological (angiogenesis and osteogenesis) and pathological (cancer, cardiovascular and autoimmune diseases) applications. The secreted analyzed factors are listed in a table. In addition, the different characteristics of the fetal MSCs derived secretome and the differences in the secretome composition of three-dimensionally (3D) cultured cells are discussed. As very innovative aspects, recent studies on some applications of engineered vesicles, embedded or not in three-dimensional structures, are treated and the influence of some epigenetic modifications on cells and EVs is investigated.
[Display omitted]</description><subject>3D cultures</subject><subject>Epigenetic</subject><subject>Extracellular vesicles</subject><subject>Mesenchymal stem cells</subject><subject>miRNAs</subject><subject>Secretome</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kMtOwzAQRS0EEqXwAeyyg02CX3nBqqrKQypiUWBruc4YuUrj4Ekq9e9JCGtWc6U5d6Q5hFwzmjDKsrtdUltMOOU8YZyWJT0hM1bkZUwzwU7JjFIuY8Fpek4uEHeU0jTNxYxsFo2uj-gw8jZ63SzxJkIwATq_h0g3VbT6xMjo8OXvo9VB173unG9G2PaNGTP-Yrpta2d-l3hJzqyuEa7-5px8PK7el8_x-u3pZblYx4Yz3sWiAFkYnVshC2sEpIIDSKuF1BkzjLNSm4pDCjbbQqEraQC2eltaK2VRUBBzcjvdbYP_7gE7tXdooK51A75HxXNWZIJnKR9QNqEmeMQAVrXB7XU4KkbVKFDt1CBQjQLVJHDoPEwdGH44OAgKjYPGQOUCmE5V3v3T_gFW73lz</recordid><startdate>20221101</startdate><enddate>20221101</enddate><creator>Urrata, Valentina</creator><creator>Trapani, Marco</creator><creator>Franza, Mara</creator><creator>Moschella, Francesco</creator><creator>Di Stefano, Anna Barbara</creator><creator>Toia, Francesca</creator><general>Elsevier Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20221101</creationdate><title>Analysis of MSCs' secretome and EVs cargo: Evaluation of functions and applications</title><author>Urrata, Valentina ; Trapani, Marco ; Franza, Mara ; Moschella, Francesco ; Di Stefano, Anna Barbara ; Toia, Francesca</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c212t-38e48ca7f348fc3e532ee4fa34a61c1219acd2e5ef6be8ad4ceebab9ff44880e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>3D cultures</topic><topic>Epigenetic</topic><topic>Extracellular vesicles</topic><topic>Mesenchymal stem cells</topic><topic>miRNAs</topic><topic>Secretome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Urrata, Valentina</creatorcontrib><creatorcontrib>Trapani, Marco</creatorcontrib><creatorcontrib>Franza, Mara</creatorcontrib><creatorcontrib>Moschella, Francesco</creatorcontrib><creatorcontrib>Di Stefano, Anna Barbara</creatorcontrib><creatorcontrib>Toia, Francesca</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Urrata, Valentina</au><au>Trapani, Marco</au><au>Franza, Mara</au><au>Moschella, Francesco</au><au>Di Stefano, Anna Barbara</au><au>Toia, Francesca</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of MSCs' secretome and EVs cargo: Evaluation of functions and applications</atitle><jtitle>Life sciences (1973)</jtitle><date>2022-11-01</date><risdate>2022</risdate><volume>308</volume><spage>120990</spage><epage>120990</epage><pages>120990-120990</pages><artnum>120990</artnum><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Mesenchymal stem cells (MSCs) can exert different functions and can be used in several medical fields. In the last years, MSC properties have been attributed to their secreted factors such as soluble proteins, cytokines and growth factors. Moreover, a key role is played by the extracellular vesicles (EVs) which lead a heterogeneous cargo of proteins, lipids and small and long non-coding RNAs that interfere with the pathways of the recipient cells. Due to the safeness and easiness in obtaining the secretome, its use is becoming a turning point for the application in physiological and pathological fields. This review summarizes the most recent studies on the use of MSCs secretome, focusing on some physiological (angiogenesis and osteogenesis) and pathological (cancer, cardiovascular and autoimmune diseases) applications. The secreted analyzed factors are listed in a table. In addition, the different characteristics of the fetal MSCs derived secretome and the differences in the secretome composition of three-dimensionally (3D) cultured cells are discussed. As very innovative aspects, recent studies on some applications of engineered vesicles, embedded or not in three-dimensional structures, are treated and the influence of some epigenetic modifications on cells and EVs is investigated.
[Display omitted]</abstract><pub>Elsevier Inc</pub><doi>10.1016/j.lfs.2022.120990</doi><tpages>1</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0024-3205 |
| ispartof | Life sciences (1973), 2022-11, Vol.308, p.120990-120990, Article 120990 |
| issn | 0024-3205 1879-0631 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2718632652 |
| source | Elsevier ScienceDirect Journals |
| subjects | 3D cultures Epigenetic Extracellular vesicles Mesenchymal stem cells miRNAs Secretome |
| title | Analysis of MSCs' secretome and EVs cargo: Evaluation of functions and applications |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T16%3A02%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Analysis%20of%20MSCs'%20secretome%20and%20EVs%20cargo:%20Evaluation%20of%20functions%20and%20applications&rft.jtitle=Life%20sciences%20(1973)&rft.au=Urrata,%20Valentina&rft.date=2022-11-01&rft.volume=308&rft.spage=120990&rft.epage=120990&rft.pages=120990-120990&rft.artnum=120990&rft.issn=0024-3205&rft.eissn=1879-0631&rft_id=info:doi/10.1016/j.lfs.2022.120990&rft_dat=%3Cproquest_cross%3E2718632652%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2718632652&rft_id=info:pmid/&rft_els_id=S0024320522006907&rfr_iscdi=true |