Urease-negative uropathogen Kalamiella piersonii YU22 metabolizes urea by urea carboxylase and allophanate hydrolase enzyme system
Urea is one of the major components of the human urine and its breakdown by the uropathogens occurs mainly through the activity of the enzyme urease. However, a few reports suggest the presence of an alternate enzyme system for urea breakdown namely urea carboxylase (UC) and allophanate hydrolase (A...
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| Veröffentlicht in: | Microbiological research 2022-10, Vol.263, p.127142-127142, Article 127142 |
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| creator | Yuvarajan, Subramaniyan Hameed, Asif Arun, Ananthapadmanabha Bhagwath Saptami, Kanekar Rekha, Punchappady Devasya |
| description | Urea is one of the major components of the human urine and its breakdown by the uropathogens occurs mainly through the activity of the enzyme urease. However, a few reports suggest the presence of an alternate enzyme system for urea breakdown namely urea carboxylase (UC) and allophanate hydrolase (AH). We have previously reported the UC and AH system in the genome of a urease-negative uropathogen Kalamiella piersonii YU22 of the novel genus Kalamiella (reclassified recently as Pantoea).To validate the UC and AH activity in the presence of urea, we investigated the growth and urea utilization patterns of this bacterium. Growth kinetics, variations in media pH, NH4-N generation and UC and AH gene expressions were probed using urea-containing media. YU22 was able to grow in M9 media containing urea and increase the pH of the media due to the urea breakdown. Further, significantly higher concentrations of extracellular NH4-N (p |
| doi_str_mv | 10.1016/j.micres.2022.127142 |
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However, a few reports suggest the presence of an alternate enzyme system for urea breakdown namely urea carboxylase (UC) and allophanate hydrolase (AH). We have previously reported the UC and AH system in the genome of a urease-negative uropathogen Kalamiella piersonii YU22 of the novel genus Kalamiella (reclassified recently as Pantoea).To validate the UC and AH activity in the presence of urea, we investigated the growth and urea utilization patterns of this bacterium. Growth kinetics, variations in media pH, NH4-N generation and UC and AH gene expressions were probed using urea-containing media. YU22 was able to grow in M9 media containing urea and increase the pH of the media due to the urea breakdown. Further, significantly higher concentrations of extracellular NH4-N (p < 0.001) was also detected in the cultures along with over-expression of UC and AH genes. The bacterium formed biofilm, and displayed swimming and swarming motilities in presence of urea. Additional glucose supply to urea boosted the colonization but ameliorated the media alkalization and ammonification through suppression of gene expressions encoding UC and AH. These results show that the urease-negative strain YU22 can utilize the UC and AH system for urea metabolism. We propose to further investigate the UC and AH system in other urease-negative uropathogens and its implications for pathogenicity and urinary tract colonization.</description><identifier>ISSN: 0944-5013</identifier><identifier>EISSN: 1618-0623</identifier><identifier>DOI: 10.1016/j.micres.2022.127142</identifier><language>eng</language><publisher>Elsevier GmbH</publisher><subject>alkalinization ; Allophanate hydrolase ; ammonification ; bacteria ; biofilm ; enzyme activity ; genes ; glucose ; growth models ; humans ; Hyperammonemia ; Kalamiella piersonii YU22 ; metabolism ; new genus ; Pantoea ; Pantoea piersonii ; pathogenicity ; urea ; Urea carboxylase ; urease ; urinary tract ; Urinary tract infection ; urine</subject><ispartof>Microbiological research, 2022-10, Vol.263, p.127142-127142, Article 127142</ispartof><rights>2022 Elsevier GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-e304ada46a985a9c630604e1839b3f11bbf1f9f7def3073573d63c0506ed02023</citedby><cites>FETCH-LOGICAL-c418t-e304ada46a985a9c630604e1839b3f11bbf1f9f7def3073573d63c0506ed02023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0944501322001823$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Yuvarajan, Subramaniyan</creatorcontrib><creatorcontrib>Hameed, Asif</creatorcontrib><creatorcontrib>Arun, Ananthapadmanabha Bhagwath</creatorcontrib><creatorcontrib>Saptami, Kanekar</creatorcontrib><creatorcontrib>Rekha, Punchappady Devasya</creatorcontrib><title>Urease-negative uropathogen Kalamiella piersonii YU22 metabolizes urea by urea carboxylase and allophanate hydrolase enzyme system</title><title>Microbiological research</title><description>Urea is one of the major components of the human urine and its breakdown by the uropathogens occurs mainly through the activity of the enzyme urease. However, a few reports suggest the presence of an alternate enzyme system for urea breakdown namely urea carboxylase (UC) and allophanate hydrolase (AH). We have previously reported the UC and AH system in the genome of a urease-negative uropathogen Kalamiella piersonii YU22 of the novel genus Kalamiella (reclassified recently as Pantoea).To validate the UC and AH activity in the presence of urea, we investigated the growth and urea utilization patterns of this bacterium. Growth kinetics, variations in media pH, NH4-N generation and UC and AH gene expressions were probed using urea-containing media. YU22 was able to grow in M9 media containing urea and increase the pH of the media due to the urea breakdown. Further, significantly higher concentrations of extracellular NH4-N (p < 0.001) was also detected in the cultures along with over-expression of UC and AH genes. The bacterium formed biofilm, and displayed swimming and swarming motilities in presence of urea. Additional glucose supply to urea boosted the colonization but ameliorated the media alkalization and ammonification through suppression of gene expressions encoding UC and AH. These results show that the urease-negative strain YU22 can utilize the UC and AH system for urea metabolism. We propose to further investigate the UC and AH system in other urease-negative uropathogens and its implications for pathogenicity and urinary tract colonization.</description><subject>alkalinization</subject><subject>Allophanate hydrolase</subject><subject>ammonification</subject><subject>bacteria</subject><subject>biofilm</subject><subject>enzyme activity</subject><subject>genes</subject><subject>glucose</subject><subject>growth models</subject><subject>humans</subject><subject>Hyperammonemia</subject><subject>Kalamiella piersonii YU22</subject><subject>metabolism</subject><subject>new genus</subject><subject>Pantoea</subject><subject>Pantoea piersonii</subject><subject>pathogenicity</subject><subject>urea</subject><subject>Urea carboxylase</subject><subject>urease</subject><subject>urinary tract</subject><subject>Urinary tract infection</subject><subject>urine</subject><issn>0944-5013</issn><issn>1618-0623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqNkTtv20AQhA9BDESR8w9SXJmG8t6DryZAYDh2YANprMLVYXlcSieQPOaOMkKV_uWmQtdGqil2ZoCdj7GvAjYCRHZ12HTOBoobCVJuhMyFlh_YSmSiSCCT6iNbQal1koJQn9jnGA8AQpeFXLGXbSCMlPS0w9E9Ez8GP-C49zvq-T222DlqW-SDoxB97xx_2krJOxqx8q07UZwThLyaFrUYKv93audOjn3NsW39sMceR-L7qQ7-34X609QRj1McqbtkFw22kb686Zptf948Xt8lD79vf13_eEisFsWYkAKNNeoMyyLF0mYKMtAkClVWqhGiqhrRlE1eU6MgV2mu6kxZSCGjGuZh1Jp9W3qH4P8cKY6mc9Gev-vJH6OZZyuULtP0f6wASqQqV7NVL1YbfIyBGjME12GYjABzpmMOZqFjznTMQmeOfV9iNH_8PI9ronXUW6pdIDua2rv3C14BmoqcNw</recordid><startdate>202210</startdate><enddate>202210</enddate><creator>Yuvarajan, Subramaniyan</creator><creator>Hameed, Asif</creator><creator>Arun, Ananthapadmanabha Bhagwath</creator><creator>Saptami, Kanekar</creator><creator>Rekha, Punchappady Devasya</creator><general>Elsevier GmbH</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>202210</creationdate><title>Urease-negative uropathogen Kalamiella piersonii YU22 metabolizes urea by urea carboxylase and allophanate hydrolase enzyme system</title><author>Yuvarajan, Subramaniyan ; Hameed, Asif ; Arun, Ananthapadmanabha Bhagwath ; Saptami, Kanekar ; Rekha, Punchappady Devasya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-e304ada46a985a9c630604e1839b3f11bbf1f9f7def3073573d63c0506ed02023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>alkalinization</topic><topic>Allophanate hydrolase</topic><topic>ammonification</topic><topic>bacteria</topic><topic>biofilm</topic><topic>enzyme activity</topic><topic>genes</topic><topic>glucose</topic><topic>growth models</topic><topic>humans</topic><topic>Hyperammonemia</topic><topic>Kalamiella piersonii YU22</topic><topic>metabolism</topic><topic>new genus</topic><topic>Pantoea</topic><topic>Pantoea piersonii</topic><topic>pathogenicity</topic><topic>urea</topic><topic>Urea carboxylase</topic><topic>urease</topic><topic>urinary tract</topic><topic>Urinary tract infection</topic><topic>urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuvarajan, Subramaniyan</creatorcontrib><creatorcontrib>Hameed, Asif</creatorcontrib><creatorcontrib>Arun, Ananthapadmanabha Bhagwath</creatorcontrib><creatorcontrib>Saptami, Kanekar</creatorcontrib><creatorcontrib>Rekha, Punchappady Devasya</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Microbiological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuvarajan, Subramaniyan</au><au>Hameed, Asif</au><au>Arun, Ananthapadmanabha Bhagwath</au><au>Saptami, Kanekar</au><au>Rekha, Punchappady Devasya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Urease-negative uropathogen Kalamiella piersonii YU22 metabolizes urea by urea carboxylase and allophanate hydrolase enzyme system</atitle><jtitle>Microbiological research</jtitle><date>2022-10</date><risdate>2022</risdate><volume>263</volume><spage>127142</spage><epage>127142</epage><pages>127142-127142</pages><artnum>127142</artnum><issn>0944-5013</issn><eissn>1618-0623</eissn><abstract>Urea is one of the major components of the human urine and its breakdown by the uropathogens occurs mainly through the activity of the enzyme urease. However, a few reports suggest the presence of an alternate enzyme system for urea breakdown namely urea carboxylase (UC) and allophanate hydrolase (AH). We have previously reported the UC and AH system in the genome of a urease-negative uropathogen Kalamiella piersonii YU22 of the novel genus Kalamiella (reclassified recently as Pantoea).To validate the UC and AH activity in the presence of urea, we investigated the growth and urea utilization patterns of this bacterium. Growth kinetics, variations in media pH, NH4-N generation and UC and AH gene expressions were probed using urea-containing media. YU22 was able to grow in M9 media containing urea and increase the pH of the media due to the urea breakdown. Further, significantly higher concentrations of extracellular NH4-N (p < 0.001) was also detected in the cultures along with over-expression of UC and AH genes. The bacterium formed biofilm, and displayed swimming and swarming motilities in presence of urea. Additional glucose supply to urea boosted the colonization but ameliorated the media alkalization and ammonification through suppression of gene expressions encoding UC and AH. These results show that the urease-negative strain YU22 can utilize the UC and AH system for urea metabolism. We propose to further investigate the UC and AH system in other urease-negative uropathogens and its implications for pathogenicity and urinary tract colonization.</abstract><pub>Elsevier GmbH</pub><doi>10.1016/j.micres.2022.127142</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | alkalinization Allophanate hydrolase ammonification bacteria biofilm enzyme activity genes glucose growth models humans Hyperammonemia Kalamiella piersonii YU22 metabolism new genus Pantoea Pantoea piersonii pathogenicity urea Urea carboxylase urease urinary tract Urinary tract infection urine |
| title | Urease-negative uropathogen Kalamiella piersonii YU22 metabolizes urea by urea carboxylase and allophanate hydrolase enzyme system |
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