Cellular Aging Secretes: a Comparison of Bone-Marrow-Derived and Induced Mesenchymal Stem Cells and Their Secretome Over Long-Term Culture
Mesenchymal stem cells (MSCs) hold promising therapeutic potential in several clinical applications, mainly due to their paracrine activity. The implementation of future secretome-based therapeutic strategies requires the use of easily accessible MSCs sources that provide high numbers of cells with...
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| Veröffentlicht in: | STEM CELL REVIEWS AND REPORTS 2023, Vol.19 (1), p.248-263 |
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| creator | Marote, Ana Santos, Diogo Mendes-Pinheiro, Bárbara Serre-Miranda, Cláudia Anjo, Sandra I. Vieira, Joana Ferreira-Antunes, Filipa Correia, Joana Sofia Borges-Pereira, Caroline Pinho, Andreia G. Campos, Jonas Manadas, Bruno Teixeira, Manuel R. Correia-Neves, Margarida Pinto, Luísa Costa, Pedro M. Roybon, Laurent Salgado, António J. |
| description | Mesenchymal stem cells (MSCs) hold promising therapeutic potential in several clinical applications, mainly due to their paracrine activity. The implementation of future secretome-based therapeutic strategies requires the use of easily accessible MSCs sources that provide high numbers of cells with homogenous characteristics. MSCs obtained from induced pluripotent stem cells (iMSCs) have been put forward as an advantageous alternative to the gold-standard tissue sources, such as bone marrow (BM-MSCs). In this study, we aimed at comparing the secretome of BM-MSCs and iMSCs over long-term culture. For that, we performed a broad characterization of both sources regarding their identity, proteomic secretome analysis, as well as replicative senescence and associated phenotypes, including its effects on MSCs secretome composition and immunomodulatory action. Our results evidence a rejuvenated phenotype of iMSCs, which is translated into a superior proliferative capacity before the induction of replicative senescence. Despite this significant difference between iMSCs and BM-MSCs proliferation, both untargeted and targeted proteomic analysis revealed a similar secretome composition for both sources in pre-senescent and senescent states. These results suggest that shifting from the use of BM-MSCs to a more advantageous source, like iMSCs, may yield similar therapeutic effects as identified over the past years for this gold-standard MSC source.
Graphical Abstract |
| doi_str_mv | 10.1007/s12015-022-10453-6 |
| format | Article |
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Despite this significant difference between iMSCs and BM-MSCs proliferation, both untargeted and targeted proteomic analysis revealed a similar secretome composition for both sources in pre-senescent and senescent states. These results suggest that shifting from the use of BM-MSCs to a more advantageous source, like iMSCs, may yield similar therapeutic effects as identified over the past years for this gold-standard MSC source.
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| ispartof | STEM CELL REVIEWS AND REPORTS, 2023, Vol.19 (1), p.248-263 |
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| subjects | Aging Basic Medicine Biomedical and Life Sciences Biomedical Engineering and Bioengineering Bone Marrow Cell and Molecular Biology Cell Biology Cell culture Cell Differentiation Cell- och molekylärbiologi Cellular Senescence Human platelet lysate Immunomodulation Induced pluripotent stem cells Life Sciences Medical and Health Sciences Medicin och hälsovetenskap Medicinska och farmaceutiska grundvetenskaper Mesenchymal Stem Cells Paracrine signalling Phenotypes Pluripotency Proteomics Regenerative Medicine/Tissue Engineering Replicative senescence Secretome Senescence Senescence-associated secretory profile Stem Cells |
| title | Cellular Aging Secretes: a Comparison of Bone-Marrow-Derived and Induced Mesenchymal Stem Cells and Their Secretome Over Long-Term Culture |
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