Dyslipidemia induced by lipid diet in late gestation donor impact on growth kinetics and in vitro potential differentiation of umbilical cord Wharton’s Jelly mesenchymal stem cells in goats
Mesenchymal stem cells (MSC) from the umbilical cord (UC) have several attractive properties for clinical use. This study aimed to verify the impact of a lipid-rich diet during late gestation of donor goats on the growth and differentiation of MSCs from UC. From the 100th day of pregnancy to deliver...
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| Veröffentlicht in: | Veterinary research communications 2022-12, Vol.46 (4), p.1259-1270 |
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| creator | Bezerra, Alessandra Façanha Alves, Juliana Paula Martins Fernandes, César Carneiro Linhares Cavalcanti, Camila Muniz Silva, Maria Raquel Lopes Conde, Alfredo José Herrera Tetaping, Gildas Mbemya Ferreira, Anna Clara Accioly Melo, Luciana Magalhães Rodrigues, Ana Paula Ribeiro Rondina, Davide |
| description | Mesenchymal stem cells (MSC) from the umbilical cord (UC) have several attractive properties for clinical use. This study aimed to verify the impact of a lipid-rich diet during late gestation of donor goats on the growth and differentiation of MSCs from UC. From the 100th day of pregnancy to delivery, 22 goats were grouped based on their diet into the donor-lipid (DLD;
n
= 11) and donor-baseline (DBD;
n
= 11) diet groups. Diets were isonitrogenous and isoenergetic, differing in fat content (2.8% vs. 6.3% on a dry matter basis). Wharton’s jelly (WJ) fragments were cultured. After primary culture, samples of WJ-MSCs were characterized by the expression of CD90, CD73, CD34, CD45, CD105, and Fas genes, mitochondrial activity using MitoTracker (MT) fluorescence probe, and growth kinetics. Population doubling time (PDT) was also determined. WJ-MSCs were differentiated into chondrocytes, adipocytes and osteocytes, and the mineralized area and adipocytes were determined. The lipid diet significantly increased triglyceride and cholesterol levels during pregnancy. The DLD group showed sub-expression of the CD90 gene, a high MT intensity, and a low proliferation rate at the end of the subculture. The mean PDT was 83.9 ± 1.3 h. Mineralized area and lipid droplet stain intensity from osteogenic and adipogenic differentiations, respectively, were greater in DLD. We conclude that in donor goats, dietary dyslipidemia during late pregnancy affects the ability of UC-derived MSCs to express their developmental potential in vitro, thus limiting their possible use for therapeutic purposes. |
| doi_str_mv | 10.1007/s11259-022-09995-4 |
| format | Article |
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n
= 11) and donor-baseline (DBD;
n
= 11) diet groups. Diets were isonitrogenous and isoenergetic, differing in fat content (2.8% vs. 6.3% on a dry matter basis). Wharton’s jelly (WJ) fragments were cultured. After primary culture, samples of WJ-MSCs were characterized by the expression of CD90, CD73, CD34, CD45, CD105, and Fas genes, mitochondrial activity using MitoTracker (MT) fluorescence probe, and growth kinetics. Population doubling time (PDT) was also determined. WJ-MSCs were differentiated into chondrocytes, adipocytes and osteocytes, and the mineralized area and adipocytes were determined. The lipid diet significantly increased triglyceride and cholesterol levels during pregnancy. The DLD group showed sub-expression of the CD90 gene, a high MT intensity, and a low proliferation rate at the end of the subculture. The mean PDT was 83.9 ± 1.3 h. Mineralized area and lipid droplet stain intensity from osteogenic and adipogenic differentiations, respectively, were greater in DLD. We conclude that in donor goats, dietary dyslipidemia during late pregnancy affects the ability of UC-derived MSCs to express their developmental potential in vitro, thus limiting their possible use for therapeutic purposes.</description><identifier>ISSN: 0165-7380</identifier><identifier>EISSN: 1573-7446</identifier><identifier>DOI: 10.1007/s11259-022-09995-4</identifier><identifier>PMID: 36125693</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adipocytes ; Animals ; Biomedical and Life Sciences ; CD105 antigen ; CD34 antigen ; CD45 antigen ; CD73 antigen ; CD90 antigen ; Cell culture ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Cholesterol ; Chondrocytes ; Diet ; Diet - veterinary ; Dry matter ; Dyslipidemia ; Dyslipidemias - metabolism ; Dyslipidemias - veterinary ; Female ; Fetal alcohol syndrome ; Gestation ; Goat Diseases ; Goats ; Kinetics ; Life Sciences ; Lipids ; Mesenchymal Stem Cells ; Metabolic disorders ; Mitochondria ; Osteocytes ; Pregnancy ; Stem cells ; Subculture ; Therapeutic applications ; Umbilical cord ; Umbilical Cord - metabolism ; Veterinary Medicine/Veterinary Science ; Wharton Jelly - metabolism ; Zoology</subject><ispartof>Veterinary research communications, 2022-12, Vol.46 (4), p.1259-1270</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2022. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c305t-7d8b4779c8eac1eca2b36296c04684a50186f3e1dcc1cae5d8b029f205c6bf993</citedby><cites>FETCH-LOGICAL-c305t-7d8b4779c8eac1eca2b36296c04684a50186f3e1dcc1cae5d8b029f205c6bf993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11259-022-09995-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11259-022-09995-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36125693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bezerra, Alessandra Façanha</creatorcontrib><creatorcontrib>Alves, Juliana Paula Martins</creatorcontrib><creatorcontrib>Fernandes, César Carneiro Linhares</creatorcontrib><creatorcontrib>Cavalcanti, Camila Muniz</creatorcontrib><creatorcontrib>Silva, Maria Raquel Lopes</creatorcontrib><creatorcontrib>Conde, Alfredo José Herrera</creatorcontrib><creatorcontrib>Tetaping, Gildas Mbemya</creatorcontrib><creatorcontrib>Ferreira, Anna Clara Accioly</creatorcontrib><creatorcontrib>Melo, Luciana Magalhães</creatorcontrib><creatorcontrib>Rodrigues, Ana Paula Ribeiro</creatorcontrib><creatorcontrib>Rondina, Davide</creatorcontrib><title>Dyslipidemia induced by lipid diet in late gestation donor impact on growth kinetics and in vitro potential differentiation of umbilical cord Wharton’s Jelly mesenchymal stem cells in goats</title><title>Veterinary research communications</title><addtitle>Vet Res Commun</addtitle><addtitle>Vet Res Commun</addtitle><description>Mesenchymal stem cells (MSC) from the umbilical cord (UC) have several attractive properties for clinical use. This study aimed to verify the impact of a lipid-rich diet during late gestation of donor goats on the growth and differentiation of MSCs from UC. From the 100th day of pregnancy to delivery, 22 goats were grouped based on their diet into the donor-lipid (DLD;
n
= 11) and donor-baseline (DBD;
n
= 11) diet groups. Diets were isonitrogenous and isoenergetic, differing in fat content (2.8% vs. 6.3% on a dry matter basis). Wharton’s jelly (WJ) fragments were cultured. After primary culture, samples of WJ-MSCs were characterized by the expression of CD90, CD73, CD34, CD45, CD105, and Fas genes, mitochondrial activity using MitoTracker (MT) fluorescence probe, and growth kinetics. Population doubling time (PDT) was also determined. WJ-MSCs were differentiated into chondrocytes, adipocytes and osteocytes, and the mineralized area and adipocytes were determined. The lipid diet significantly increased triglyceride and cholesterol levels during pregnancy. The DLD group showed sub-expression of the CD90 gene, a high MT intensity, and a low proliferation rate at the end of the subculture. The mean PDT was 83.9 ± 1.3 h. Mineralized area and lipid droplet stain intensity from osteogenic and adipogenic differentiations, respectively, were greater in DLD. We conclude that in donor goats, dietary dyslipidemia during late pregnancy affects the ability of UC-derived MSCs to express their developmental potential in vitro, thus limiting their possible use for therapeutic purposes.</description><subject>Adipocytes</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>CD105 antigen</subject><subject>CD34 antigen</subject><subject>CD45 antigen</subject><subject>CD73 antigen</subject><subject>CD90 antigen</subject><subject>Cell culture</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Cholesterol</subject><subject>Chondrocytes</subject><subject>Diet</subject><subject>Diet - veterinary</subject><subject>Dry matter</subject><subject>Dyslipidemia</subject><subject>Dyslipidemias - metabolism</subject><subject>Dyslipidemias - veterinary</subject><subject>Female</subject><subject>Fetal alcohol syndrome</subject><subject>Gestation</subject><subject>Goat Diseases</subject><subject>Goats</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Mesenchymal Stem Cells</subject><subject>Metabolic disorders</subject><subject>Mitochondria</subject><subject>Osteocytes</subject><subject>Pregnancy</subject><subject>Stem cells</subject><subject>Subculture</subject><subject>Therapeutic applications</subject><subject>Umbilical cord</subject><subject>Umbilical Cord - metabolism</subject><subject>Veterinary Medicine/Veterinary Science</subject><subject>Wharton Jelly - metabolism</subject><subject>Zoology</subject><issn>0165-7380</issn><issn>1573-7446</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUuO1DAQhi0EYpqBC7BAltiwCfiROPESzfDUSGxALCPHrnR7iO1gO6DsuAan4S6cBKd7AIkFq1KVv_ps-UfoISVPKSHts0Qpa2RFGKuIlLKp6ltoR5uWV21di9toR6hoqpZ35AzdS-maECI7wu-iMy7KppB8h35crmmyszXgrMLWm0WDwcOKj0NsLOQyxZPKgPeQsso2eGyCDxFbNyudcen3MXzNB_zJeshWJ6y82ba-2BwDnkMGn62aim0cIR6boyaMeHGDnawuhzpEgz8eVMzB__z2PeG3ME0rdpDA68PqCpIyOKzLOG32fVA53Ud3RjUleHBTz9GHly_eX7yurt69enPx_KrSnDS5ak031G0rdQdKU9CKDVwwKTSpRVerhtBOjByo0ZpqBU3BCZMjI40WwyglP0dPTt45hs9L-Yje2bQ9RXkIS-pZSwWRxVYX9PE_6HVYoi-vKxSXnLFSCsVOlI4hpQhjP0frVFx7Svot3v4Ub1_i7Y_x9pv60Y16GRyYPyu_8ywAPwGpHPk9xL93_0f7C7uQtbM</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Bezerra, Alessandra Façanha</creator><creator>Alves, Juliana Paula Martins</creator><creator>Fernandes, César Carneiro Linhares</creator><creator>Cavalcanti, Camila Muniz</creator><creator>Silva, Maria Raquel Lopes</creator><creator>Conde, Alfredo José Herrera</creator><creator>Tetaping, Gildas Mbemya</creator><creator>Ferreira, Anna Clara Accioly</creator><creator>Melo, Luciana Magalhães</creator><creator>Rodrigues, Ana Paula Ribeiro</creator><creator>Rondina, Davide</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20221201</creationdate><title>Dyslipidemia induced by lipid diet in late gestation donor impact on growth kinetics and in vitro potential differentiation of umbilical cord Wharton’s Jelly mesenchymal stem cells in goats</title><author>Bezerra, Alessandra Façanha ; Alves, Juliana Paula Martins ; Fernandes, César Carneiro Linhares ; Cavalcanti, Camila Muniz ; Silva, Maria Raquel Lopes ; Conde, Alfredo José Herrera ; Tetaping, Gildas Mbemya ; Ferreira, Anna Clara Accioly ; Melo, Luciana Magalhães ; Rodrigues, Ana Paula Ribeiro ; Rondina, Davide</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-7d8b4779c8eac1eca2b36296c04684a50186f3e1dcc1cae5d8b029f205c6bf993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adipocytes</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>CD105 antigen</topic><topic>CD34 antigen</topic><topic>CD45 antigen</topic><topic>CD73 antigen</topic><topic>CD90 antigen</topic><topic>Cell culture</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Cholesterol</topic><topic>Chondrocytes</topic><topic>Diet</topic><topic>Diet - veterinary</topic><topic>Dry matter</topic><topic>Dyslipidemia</topic><topic>Dyslipidemias - metabolism</topic><topic>Dyslipidemias - veterinary</topic><topic>Female</topic><topic>Fetal alcohol syndrome</topic><topic>Gestation</topic><topic>Goat Diseases</topic><topic>Goats</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Mesenchymal Stem Cells</topic><topic>Metabolic disorders</topic><topic>Mitochondria</topic><topic>Osteocytes</topic><topic>Pregnancy</topic><topic>Stem cells</topic><topic>Subculture</topic><topic>Therapeutic applications</topic><topic>Umbilical cord</topic><topic>Umbilical Cord - metabolism</topic><topic>Veterinary Medicine/Veterinary Science</topic><topic>Wharton Jelly - metabolism</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bezerra, Alessandra Façanha</creatorcontrib><creatorcontrib>Alves, Juliana Paula Martins</creatorcontrib><creatorcontrib>Fernandes, César Carneiro Linhares</creatorcontrib><creatorcontrib>Cavalcanti, Camila Muniz</creatorcontrib><creatorcontrib>Silva, Maria Raquel Lopes</creatorcontrib><creatorcontrib>Conde, Alfredo José Herrera</creatorcontrib><creatorcontrib>Tetaping, Gildas Mbemya</creatorcontrib><creatorcontrib>Ferreira, Anna Clara Accioly</creatorcontrib><creatorcontrib>Melo, Luciana Magalhães</creatorcontrib><creatorcontrib>Rodrigues, Ana Paula Ribeiro</creatorcontrib><creatorcontrib>Rondina, Davide</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bezerra, Alessandra Façanha</au><au>Alves, Juliana Paula Martins</au><au>Fernandes, César Carneiro Linhares</au><au>Cavalcanti, Camila Muniz</au><au>Silva, Maria Raquel Lopes</au><au>Conde, Alfredo José Herrera</au><au>Tetaping, Gildas Mbemya</au><au>Ferreira, Anna Clara Accioly</au><au>Melo, Luciana Magalhães</au><au>Rodrigues, Ana Paula Ribeiro</au><au>Rondina, Davide</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dyslipidemia induced by lipid diet in late gestation donor impact on growth kinetics and in vitro potential differentiation of umbilical cord Wharton’s Jelly mesenchymal stem cells in goats</atitle><jtitle>Veterinary research communications</jtitle><stitle>Vet Res Commun</stitle><addtitle>Vet Res Commun</addtitle><date>2022-12-01</date><risdate>2022</risdate><volume>46</volume><issue>4</issue><spage>1259</spage><epage>1270</epage><pages>1259-1270</pages><issn>0165-7380</issn><eissn>1573-7446</eissn><abstract>Mesenchymal stem cells (MSC) from the umbilical cord (UC) have several attractive properties for clinical use. This study aimed to verify the impact of a lipid-rich diet during late gestation of donor goats on the growth and differentiation of MSCs from UC. From the 100th day of pregnancy to delivery, 22 goats were grouped based on their diet into the donor-lipid (DLD;
n
= 11) and donor-baseline (DBD;
n
= 11) diet groups. Diets were isonitrogenous and isoenergetic, differing in fat content (2.8% vs. 6.3% on a dry matter basis). Wharton’s jelly (WJ) fragments were cultured. After primary culture, samples of WJ-MSCs were characterized by the expression of CD90, CD73, CD34, CD45, CD105, and Fas genes, mitochondrial activity using MitoTracker (MT) fluorescence probe, and growth kinetics. Population doubling time (PDT) was also determined. WJ-MSCs were differentiated into chondrocytes, adipocytes and osteocytes, and the mineralized area and adipocytes were determined. The lipid diet significantly increased triglyceride and cholesterol levels during pregnancy. The DLD group showed sub-expression of the CD90 gene, a high MT intensity, and a low proliferation rate at the end of the subculture. The mean PDT was 83.9 ± 1.3 h. Mineralized area and lipid droplet stain intensity from osteogenic and adipogenic differentiations, respectively, were greater in DLD. We conclude that in donor goats, dietary dyslipidemia during late pregnancy affects the ability of UC-derived MSCs to express their developmental potential in vitro, thus limiting their possible use for therapeutic purposes.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>36125693</pmid><doi>10.1007/s11259-022-09995-4</doi><tpages>12</tpages></addata></record> |
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| ispartof | Veterinary research communications, 2022-12, Vol.46 (4), p.1259-1270 |
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| subjects | Adipocytes Animals Biomedical and Life Sciences CD105 antigen CD34 antigen CD45 antigen CD73 antigen CD90 antigen Cell culture Cell Differentiation Cell Proliferation Cells, Cultured Cholesterol Chondrocytes Diet Diet - veterinary Dry matter Dyslipidemia Dyslipidemias - metabolism Dyslipidemias - veterinary Female Fetal alcohol syndrome Gestation Goat Diseases Goats Kinetics Life Sciences Lipids Mesenchymal Stem Cells Metabolic disorders Mitochondria Osteocytes Pregnancy Stem cells Subculture Therapeutic applications Umbilical cord Umbilical Cord - metabolism Veterinary Medicine/Veterinary Science Wharton Jelly - metabolism Zoology |
| title | Dyslipidemia induced by lipid diet in late gestation donor impact on growth kinetics and in vitro potential differentiation of umbilical cord Wharton’s Jelly mesenchymal stem cells in goats |
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