Ropivacaine represses the ovarian cancer cell stemness and facilitates cell ferroptosis through inactivating the PI3K/AKT signaling pathway
Purpose Ovarian cancer is a malignant tumor in women all over the world. Ropivacaine is identified as a potential drug for the treatment of malignant tumors, but the role and mechanism of ropivacaine in ovarian cancer remains unknown. Materials and methods Ovarian cancer cells were treated with diff...
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| Veröffentlicht in: | Human & experimental toxicology 2022-08, Vol.41, p.9603271221120652-9603271221120652 |
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| container_title | Human & experimental toxicology |
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| creator | Lu, Yi Mao, Jinbao Xu, Yanbing Pan, Hao Wang, Yu Li, Wei |
| description | Purpose
Ovarian cancer is a malignant tumor in women all over the world. Ropivacaine is identified as a potential drug for the treatment of malignant tumors, but the role and mechanism of ropivacaine in ovarian cancer remains unknown.
Materials and methods
Ovarian cancer cells were treated with different doses of ropivacaine. The function of ropivacaine in ovarian cancer was assessed using Cell Counting Kit-8 assay, flow cytometry, sphere-formation assay, Western blot, Fe2+ level analysis, and immunofluorescence. Meanwhile, the mechanism of ropivacaine in ovarian cancer was investigated by multiple molecular experiments. The protective function of ropivacaine in ovarian cancer was further confirmed by in vivo assay.
Results
The functional research data indicated that the growth and stemness of ovarian cancer cells were restrained after ropivacaine treatment, while the ferroptosis in ovarian cancer cells was facilitated. The mechanism results confirmed that ropivacaine inactivated the PI3K/AKT signaling pathway in ovarian cancer cells. Furthermore, in vivo assay demonstrated that ropivacaine repressed the proliferation of ovarian cancer cells in vivo and had a protective function in ovarian cancer.
Conclusion
Ropivacaine restrained ovarian cancer cell stemness and accelerated cell ferroptosis by inactivating PI3K/AKT signaling pathway. |
| doi_str_mv | 10.1177/09603271221120652 |
| format | Article |
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Ovarian cancer is a malignant tumor in women all over the world. Ropivacaine is identified as a potential drug for the treatment of malignant tumors, but the role and mechanism of ropivacaine in ovarian cancer remains unknown.
Materials and methods
Ovarian cancer cells were treated with different doses of ropivacaine. The function of ropivacaine in ovarian cancer was assessed using Cell Counting Kit-8 assay, flow cytometry, sphere-formation assay, Western blot, Fe2+ level analysis, and immunofluorescence. Meanwhile, the mechanism of ropivacaine in ovarian cancer was investigated by multiple molecular experiments. The protective function of ropivacaine in ovarian cancer was further confirmed by in vivo assay.
Results
The functional research data indicated that the growth and stemness of ovarian cancer cells were restrained after ropivacaine treatment, while the ferroptosis in ovarian cancer cells was facilitated. The mechanism results confirmed that ropivacaine inactivated the PI3K/AKT signaling pathway in ovarian cancer cells. Furthermore, in vivo assay demonstrated that ropivacaine repressed the proliferation of ovarian cancer cells in vivo and had a protective function in ovarian cancer.
Conclusion
Ropivacaine restrained ovarian cancer cell stemness and accelerated cell ferroptosis by inactivating PI3K/AKT signaling pathway.</description><identifier>ISSN: 0960-3271</identifier><identifier>EISSN: 1477-0903</identifier><identifier>DOI: 10.1177/09603271221120652</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>1-Phosphatidylinositol 3-kinase ; AKT protein ; Assaying ; Cancer ; Cell proliferation ; Deactivation ; Ferroptosis ; Flow cytometry ; Immunofluorescence ; Ovarian cancer ; Ropivacaine ; Signal transduction ; Tumors</subject><ispartof>Human & experimental toxicology, 2022-08, Vol.41, p.9603271221120652-9603271221120652</ispartof><rights>The Author(s) 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-4d1c1ab91bb553e18d799a6f679f3dc489763533ae1054dcd3e7a5ed56b12e9c3</citedby><cites>FETCH-LOGICAL-c388t-4d1c1ab91bb553e18d799a6f679f3dc489763533ae1054dcd3e7a5ed56b12e9c3</cites><orcidid>0000-0002-4502-5813</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/09603271221120652$$EPDF$$P50$$Gsage$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/09603271221120652$$EHTML$$P50$$Gsage$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,864,21966,27853,27924,27925,44945,45333</link.rule.ids></links><search><creatorcontrib>Lu, Yi</creatorcontrib><creatorcontrib>Mao, Jinbao</creatorcontrib><creatorcontrib>Xu, Yanbing</creatorcontrib><creatorcontrib>Pan, Hao</creatorcontrib><creatorcontrib>Wang, Yu</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><title>Ropivacaine represses the ovarian cancer cell stemness and facilitates cell ferroptosis through inactivating the PI3K/AKT signaling pathway</title><title>Human & experimental toxicology</title><addtitle>Hum Exp Toxicol</addtitle><description>Purpose
Ovarian cancer is a malignant tumor in women all over the world. Ropivacaine is identified as a potential drug for the treatment of malignant tumors, but the role and mechanism of ropivacaine in ovarian cancer remains unknown.
Materials and methods
Ovarian cancer cells were treated with different doses of ropivacaine. The function of ropivacaine in ovarian cancer was assessed using Cell Counting Kit-8 assay, flow cytometry, sphere-formation assay, Western blot, Fe2+ level analysis, and immunofluorescence. Meanwhile, the mechanism of ropivacaine in ovarian cancer was investigated by multiple molecular experiments. The protective function of ropivacaine in ovarian cancer was further confirmed by in vivo assay.
Results
The functional research data indicated that the growth and stemness of ovarian cancer cells were restrained after ropivacaine treatment, while the ferroptosis in ovarian cancer cells was facilitated. The mechanism results confirmed that ropivacaine inactivated the PI3K/AKT signaling pathway in ovarian cancer cells. Furthermore, in vivo assay demonstrated that ropivacaine repressed the proliferation of ovarian cancer cells in vivo and had a protective function in ovarian cancer.
Conclusion
Ropivacaine restrained ovarian cancer cell stemness and accelerated cell ferroptosis by inactivating PI3K/AKT signaling pathway.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>AKT protein</subject><subject>Assaying</subject><subject>Cancer</subject><subject>Cell proliferation</subject><subject>Deactivation</subject><subject>Ferroptosis</subject><subject>Flow cytometry</subject><subject>Immunofluorescence</subject><subject>Ovarian cancer</subject><subject>Ropivacaine</subject><subject>Signal transduction</subject><subject>Tumors</subject><issn>0960-3271</issn><issn>1477-0903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>AFRWT</sourceid><recordid>eNp1kV1LHDEUhkNR6Nb2B_Qu0JvejCaTzdeliK2i0FL0ejibObMbmU2mSVbxN_RPm3ELguLVgXOe9z1fhHzl7JhzrU-YVUy0mrct5y1Tsv1AFnypdcMsEwdkMdebGfhIPuV8xxhTVvIF-fcnTv4eHPiANOGUMGfMtGyQxntIHgJ1EBwm6nAcaS64DRWhEHo6gPOjL1Cq4Lk6YEpxKjH72SHF3XpDfQBXaofiw_rZ9veluDo5vbqh2a8DjHN6grJ5gMfP5HCAMeOX__GI3P44vzm7aK5__bw8O71unDCmNMueOw4ry1crKQVy02trQQ1K20H0bmmsVkIKAciZXPauF6hBYi_VirdonTgi3_e-U4p_d5hLt_V5XgACxl3u6pUUM0YbUdFvr9C7uEt17EoZq4zmQupK8T3lUsw54dBNyW8hPXacdfN7ujfvqZrjvSbDGl9c3xc8AdN_kWI</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>Lu, Yi</creator><creator>Mao, Jinbao</creator><creator>Xu, Yanbing</creator><creator>Pan, Hao</creator><creator>Wang, Yu</creator><creator>Li, Wei</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><scope>AFRWT</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4502-5813</orcidid></search><sort><creationdate>20220801</creationdate><title>Ropivacaine represses the ovarian cancer cell stemness and facilitates cell ferroptosis through inactivating the PI3K/AKT signaling pathway</title><author>Lu, Yi ; Mao, Jinbao ; Xu, Yanbing ; Pan, Hao ; Wang, Yu ; Li, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-4d1c1ab91bb553e18d799a6f679f3dc489763533ae1054dcd3e7a5ed56b12e9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>AKT protein</topic><topic>Assaying</topic><topic>Cancer</topic><topic>Cell proliferation</topic><topic>Deactivation</topic><topic>Ferroptosis</topic><topic>Flow cytometry</topic><topic>Immunofluorescence</topic><topic>Ovarian cancer</topic><topic>Ropivacaine</topic><topic>Signal transduction</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Yi</creatorcontrib><creatorcontrib>Mao, Jinbao</creatorcontrib><creatorcontrib>Xu, Yanbing</creatorcontrib><creatorcontrib>Pan, Hao</creatorcontrib><creatorcontrib>Wang, Yu</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><collection>Sage Journals GOLD Open Access 2024</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human & experimental toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Yi</au><au>Mao, Jinbao</au><au>Xu, Yanbing</au><au>Pan, Hao</au><au>Wang, Yu</au><au>Li, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ropivacaine represses the ovarian cancer cell stemness and facilitates cell ferroptosis through inactivating the PI3K/AKT signaling pathway</atitle><jtitle>Human & experimental toxicology</jtitle><addtitle>Hum Exp Toxicol</addtitle><date>2022-08-01</date><risdate>2022</risdate><volume>41</volume><spage>9603271221120652</spage><epage>9603271221120652</epage><pages>9603271221120652-9603271221120652</pages><issn>0960-3271</issn><eissn>1477-0903</eissn><abstract>Purpose
Ovarian cancer is a malignant tumor in women all over the world. Ropivacaine is identified as a potential drug for the treatment of malignant tumors, but the role and mechanism of ropivacaine in ovarian cancer remains unknown.
Materials and methods
Ovarian cancer cells were treated with different doses of ropivacaine. The function of ropivacaine in ovarian cancer was assessed using Cell Counting Kit-8 assay, flow cytometry, sphere-formation assay, Western blot, Fe2+ level analysis, and immunofluorescence. Meanwhile, the mechanism of ropivacaine in ovarian cancer was investigated by multiple molecular experiments. The protective function of ropivacaine in ovarian cancer was further confirmed by in vivo assay.
Results
The functional research data indicated that the growth and stemness of ovarian cancer cells were restrained after ropivacaine treatment, while the ferroptosis in ovarian cancer cells was facilitated. The mechanism results confirmed that ropivacaine inactivated the PI3K/AKT signaling pathway in ovarian cancer cells. Furthermore, in vivo assay demonstrated that ropivacaine repressed the proliferation of ovarian cancer cells in vivo and had a protective function in ovarian cancer.
Conclusion
Ropivacaine restrained ovarian cancer cell stemness and accelerated cell ferroptosis by inactivating PI3K/AKT signaling pathway.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><doi>10.1177/09603271221120652</doi><orcidid>https://orcid.org/0000-0002-4502-5813</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Sage Journals GOLD Open Access 2024; Alma/SFX Local Collection |
| subjects | 1-Phosphatidylinositol 3-kinase AKT protein Assaying Cancer Cell proliferation Deactivation Ferroptosis Flow cytometry Immunofluorescence Ovarian cancer Ropivacaine Signal transduction Tumors |
| title | Ropivacaine represses the ovarian cancer cell stemness and facilitates cell ferroptosis through inactivating the PI3K/AKT signaling pathway |
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