Injectable Carrier-Free Hydrogel Dressing with Anti-Multidrug-Resistant Staphylococcus aureus and Anti-Inflammatory Capabilities for Accelerated Wound Healing
Antibacterial hydrogels have gradually become a powerful weapon to treat bacterially infected wounds and accelerate healing. In this paper, we designed a small-molecule self-healing antibacterial hydrogel containing 100% drug-loaded benzyl 3β-amino-11-oxo-olean-12-en-30-oate (GN-Bn), which was gover...
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Veröffentlicht in: | ACS applied materials & interfaces 2022-09, Vol.14 (38), p.43035-43049 |
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creator | Cai, Desheng Yang, Yuqin Lu, Jihui Yuan, Zhihua Zhang, Yaozhi Yang, Xiaoyun Huang, Xuemei Li, Tong Tian, Xuehao Xu, Bing Wang, Penglong Lei, Haimin |
description | Antibacterial hydrogels have gradually become a powerful weapon to treat bacterially infected wounds and accelerate healing. In this paper, we designed a small-molecule self-healing antibacterial hydrogel containing 100% drug-loaded benzyl 3β-amino-11-oxo-olean-12-en-30-oate (GN-Bn), which was governed by π–π stacking, hydrogen bonding, and van der Waals forces. Due to the carrier-free design concept, the problems of interbatch variability during sample preparation and carrier-related toxicity can be effectively avoided. Moreover, the GN-Bn hydrogel exhibited promising antibacterial activities against multidrug-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration (MIC) of the GN-Bn hydrogel was 1.5625 nmol/mL, which was lower than those against clinical agents such as norfloxacin, penicillin, and tetracycline. This is attributed to its unique antibacterial mechanism that aims at killing bacteria or preventing their growth by regulating arginine biosynthesis and metabolism through both transcriptomic (RNA-seq) analysis and quantitative polymerase chain reaction (qPCR) analysis. In addition, the GN-Bn hydrogel can also inhibit proinflammatory cytokines (TNF-α, IL-1β, and IL-6) to promote wound healing. Collectively, the GN-Bn hydrogel elicited dual therapeutic effects on an MRSA-infected full-thickness skin wound model through its antibacterial and anti-inflammatory activities, which is attributed to the fact that the GN-Bn hydrogel has multiple advantages including sufficient mechanical stability, biocompatibility, and unique antibacterial mechanisms, making it significantly accelerate MRSA-infected full-thickness skin wound healing as a wound dressing. In a word, the GN-Bn antibacterial hydrogel dressing with an anti-inflammatory and antibacterial bifunctional material holds great potential in clinical application. |
doi_str_mv | 10.1021/acsami.2c15463 |
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In this paper, we designed a small-molecule self-healing antibacterial hydrogel containing 100% drug-loaded benzyl 3β-amino-11-oxo-olean-12-en-30-oate (GN-Bn), which was governed by π–π stacking, hydrogen bonding, and van der Waals forces. Due to the carrier-free design concept, the problems of interbatch variability during sample preparation and carrier-related toxicity can be effectively avoided. Moreover, the GN-Bn hydrogel exhibited promising antibacterial activities against multidrug-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration (MIC) of the GN-Bn hydrogel was 1.5625 nmol/mL, which was lower than those against clinical agents such as norfloxacin, penicillin, and tetracycline. This is attributed to its unique antibacterial mechanism that aims at killing bacteria or preventing their growth by regulating arginine biosynthesis and metabolism through both transcriptomic (RNA-seq) analysis and quantitative polymerase chain reaction (qPCR) analysis. In addition, the GN-Bn hydrogel can also inhibit proinflammatory cytokines (TNF-α, IL-1β, and IL-6) to promote wound healing. Collectively, the GN-Bn hydrogel elicited dual therapeutic effects on an MRSA-infected full-thickness skin wound model through its antibacterial and anti-inflammatory activities, which is attributed to the fact that the GN-Bn hydrogel has multiple advantages including sufficient mechanical stability, biocompatibility, and unique antibacterial mechanisms, making it significantly accelerate MRSA-infected full-thickness skin wound healing as a wound dressing. In a word, the GN-Bn antibacterial hydrogel dressing with an anti-inflammatory and antibacterial bifunctional material holds great potential in clinical application.</description><identifier>ISSN: 1944-8244</identifier><identifier>EISSN: 1944-8252</identifier><identifier>DOI: 10.1021/acsami.2c15463</identifier><language>eng</language><publisher>American Chemical Society</publisher><subject>Biological and Medical Applications of Materials and Interfaces</subject><ispartof>ACS applied materials & interfaces, 2022-09, Vol.14 (38), p.43035-43049</ispartof><rights>2022 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a307t-8588e4d3d075889c4c9dcc93ab80bf0bbd9ef8e041ec368df36fdfb9a037cc393</citedby><cites>FETCH-LOGICAL-a307t-8588e4d3d075889c4c9dcc93ab80bf0bbd9ef8e041ec368df36fdfb9a037cc393</cites><orcidid>0000-0001-8952-6428</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acsami.2c15463$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acsami.2c15463$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids></links><search><creatorcontrib>Cai, Desheng</creatorcontrib><creatorcontrib>Yang, Yuqin</creatorcontrib><creatorcontrib>Lu, Jihui</creatorcontrib><creatorcontrib>Yuan, Zhihua</creatorcontrib><creatorcontrib>Zhang, Yaozhi</creatorcontrib><creatorcontrib>Yang, Xiaoyun</creatorcontrib><creatorcontrib>Huang, Xuemei</creatorcontrib><creatorcontrib>Li, Tong</creatorcontrib><creatorcontrib>Tian, Xuehao</creatorcontrib><creatorcontrib>Xu, Bing</creatorcontrib><creatorcontrib>Wang, Penglong</creatorcontrib><creatorcontrib>Lei, Haimin</creatorcontrib><title>Injectable Carrier-Free Hydrogel Dressing with Anti-Multidrug-Resistant Staphylococcus aureus and Anti-Inflammatory Capabilities for Accelerated Wound Healing</title><title>ACS applied materials & interfaces</title><addtitle>ACS Appl. Mater. Interfaces</addtitle><description>Antibacterial hydrogels have gradually become a powerful weapon to treat bacterially infected wounds and accelerate healing. In this paper, we designed a small-molecule self-healing antibacterial hydrogel containing 100% drug-loaded benzyl 3β-amino-11-oxo-olean-12-en-30-oate (GN-Bn), which was governed by π–π stacking, hydrogen bonding, and van der Waals forces. Due to the carrier-free design concept, the problems of interbatch variability during sample preparation and carrier-related toxicity can be effectively avoided. Moreover, the GN-Bn hydrogel exhibited promising antibacterial activities against multidrug-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration (MIC) of the GN-Bn hydrogel was 1.5625 nmol/mL, which was lower than those against clinical agents such as norfloxacin, penicillin, and tetracycline. This is attributed to its unique antibacterial mechanism that aims at killing bacteria or preventing their growth by regulating arginine biosynthesis and metabolism through both transcriptomic (RNA-seq) analysis and quantitative polymerase chain reaction (qPCR) analysis. In addition, the GN-Bn hydrogel can also inhibit proinflammatory cytokines (TNF-α, IL-1β, and IL-6) to promote wound healing. Collectively, the GN-Bn hydrogel elicited dual therapeutic effects on an MRSA-infected full-thickness skin wound model through its antibacterial and anti-inflammatory activities, which is attributed to the fact that the GN-Bn hydrogel has multiple advantages including sufficient mechanical stability, biocompatibility, and unique antibacterial mechanisms, making it significantly accelerate MRSA-infected full-thickness skin wound healing as a wound dressing. In a word, the GN-Bn antibacterial hydrogel dressing with an anti-inflammatory and antibacterial bifunctional material holds great potential in clinical application.</description><subject>Biological and Medical Applications of Materials and Interfaces</subject><issn>1944-8244</issn><issn>1944-8252</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1UU1r3DAQNaWBpEmvOetYCt7Klj_k47LtdhcSAvmgRzOWRrtaZGs7kgn7Z_pb68Uht5zeY3hv3gwvSW4zvsh4nv0AFaC3i1xlZVGJT8lV1hRFKvMy__zOi-Iy-RLCgfNK5Ly8Sv5thwOqCJ1DtgIii5SuCZFtTpr8Dh37SRiCHXbs1cY9Ww7Rpveji1bTuEsfMdgQYYjsKcJxf3JeeaXGwGAkPMOgZ8t2MA76HqKn0xR0hM46Gy0GZjyxpVLokCCiZn_8OJk2CG4KvUkuDLiAX9_wOnlZ_3pebdK7h9_b1fIuBcHrmMpSSiy00LyeWKMK1WilGgGd5J3hXacbNBJ5kaESldRGVEabrgEuaqVEI66Tb_PeI_m_I4bY9jZMNzkY0I-hzeus4lLW5Vm6mKWKfAiEpj2S7YFObcbbcxHtXET7VsRk-D4bpnl78CMN0ycfif8Dpc6Puw</recordid><startdate>20220928</startdate><enddate>20220928</enddate><creator>Cai, Desheng</creator><creator>Yang, Yuqin</creator><creator>Lu, Jihui</creator><creator>Yuan, Zhihua</creator><creator>Zhang, Yaozhi</creator><creator>Yang, Xiaoyun</creator><creator>Huang, Xuemei</creator><creator>Li, Tong</creator><creator>Tian, Xuehao</creator><creator>Xu, Bing</creator><creator>Wang, Penglong</creator><creator>Lei, Haimin</creator><general>American Chemical Society</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8952-6428</orcidid></search><sort><creationdate>20220928</creationdate><title>Injectable Carrier-Free Hydrogel Dressing with Anti-Multidrug-Resistant Staphylococcus aureus and Anti-Inflammatory Capabilities for Accelerated Wound Healing</title><author>Cai, Desheng ; Yang, Yuqin ; Lu, Jihui ; Yuan, Zhihua ; Zhang, Yaozhi ; Yang, Xiaoyun ; Huang, Xuemei ; Li, Tong ; Tian, Xuehao ; Xu, Bing ; Wang, Penglong ; Lei, Haimin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a307t-8588e4d3d075889c4c9dcc93ab80bf0bbd9ef8e041ec368df36fdfb9a037cc393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biological and Medical Applications of Materials and Interfaces</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cai, Desheng</creatorcontrib><creatorcontrib>Yang, Yuqin</creatorcontrib><creatorcontrib>Lu, Jihui</creatorcontrib><creatorcontrib>Yuan, Zhihua</creatorcontrib><creatorcontrib>Zhang, Yaozhi</creatorcontrib><creatorcontrib>Yang, Xiaoyun</creatorcontrib><creatorcontrib>Huang, Xuemei</creatorcontrib><creatorcontrib>Li, Tong</creatorcontrib><creatorcontrib>Tian, Xuehao</creatorcontrib><creatorcontrib>Xu, Bing</creatorcontrib><creatorcontrib>Wang, Penglong</creatorcontrib><creatorcontrib>Lei, Haimin</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS applied materials & interfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cai, Desheng</au><au>Yang, Yuqin</au><au>Lu, Jihui</au><au>Yuan, Zhihua</au><au>Zhang, Yaozhi</au><au>Yang, Xiaoyun</au><au>Huang, Xuemei</au><au>Li, Tong</au><au>Tian, Xuehao</au><au>Xu, Bing</au><au>Wang, Penglong</au><au>Lei, Haimin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Injectable Carrier-Free Hydrogel Dressing with Anti-Multidrug-Resistant Staphylococcus aureus and Anti-Inflammatory Capabilities for Accelerated Wound Healing</atitle><jtitle>ACS applied materials & interfaces</jtitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><date>2022-09-28</date><risdate>2022</risdate><volume>14</volume><issue>38</issue><spage>43035</spage><epage>43049</epage><pages>43035-43049</pages><issn>1944-8244</issn><eissn>1944-8252</eissn><abstract>Antibacterial hydrogels have gradually become a powerful weapon to treat bacterially infected wounds and accelerate healing. In this paper, we designed a small-molecule self-healing antibacterial hydrogel containing 100% drug-loaded benzyl 3β-amino-11-oxo-olean-12-en-30-oate (GN-Bn), which was governed by π–π stacking, hydrogen bonding, and van der Waals forces. Due to the carrier-free design concept, the problems of interbatch variability during sample preparation and carrier-related toxicity can be effectively avoided. Moreover, the GN-Bn hydrogel exhibited promising antibacterial activities against multidrug-resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration (MIC) of the GN-Bn hydrogel was 1.5625 nmol/mL, which was lower than those against clinical agents such as norfloxacin, penicillin, and tetracycline. This is attributed to its unique antibacterial mechanism that aims at killing bacteria or preventing their growth by regulating arginine biosynthesis and metabolism through both transcriptomic (RNA-seq) analysis and quantitative polymerase chain reaction (qPCR) analysis. In addition, the GN-Bn hydrogel can also inhibit proinflammatory cytokines (TNF-α, IL-1β, and IL-6) to promote wound healing. Collectively, the GN-Bn hydrogel elicited dual therapeutic effects on an MRSA-infected full-thickness skin wound model through its antibacterial and anti-inflammatory activities, which is attributed to the fact that the GN-Bn hydrogel has multiple advantages including sufficient mechanical stability, biocompatibility, and unique antibacterial mechanisms, making it significantly accelerate MRSA-infected full-thickness skin wound healing as a wound dressing. In a word, the GN-Bn antibacterial hydrogel dressing with an anti-inflammatory and antibacterial bifunctional material holds great potential in clinical application.</abstract><pub>American Chemical Society</pub><doi>10.1021/acsami.2c15463</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-8952-6428</orcidid></addata></record> |
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title | Injectable Carrier-Free Hydrogel Dressing with Anti-Multidrug-Resistant Staphylococcus aureus and Anti-Inflammatory Capabilities for Accelerated Wound Healing |
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