Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro
OBJECTIVEto investigate the ability of solutions containing sodium hexametaphosphate, fluoride and quercetin, alone or in association, to prevent dentin erosion and to inhibit matrix metalloproteinases -2 and -9 activity using in vitro protocols. DESIGNRoot dentin blocks (n = 96) were prepared and d...
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| Veröffentlicht in: | Archives of oral biology 2022-11, Vol.143, p.105541-105541, Article 105541 |
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| container_title | Archives of oral biology |
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| creator | Capalbo, Letícia Cabrera Delbem, Alberto Carlos Botazzo Dal-Fabbro, Renan Inácio, Kelly Karina de Oliveira, Rodrigo Cardoso Pessan, Juliano Pelim |
| description | OBJECTIVEto investigate the ability of solutions containing sodium hexametaphosphate, fluoride and quercetin, alone or in association, to prevent dentin erosion and to inhibit matrix metalloproteinases -2 and -9 activity using in vitro protocols. DESIGNRoot dentin blocks (n = 96) were prepared and divided into 8 experimental groups (n = 12/group), according to the solutions to be tested: Placebo; 0.24% sodium fluoride (F); 1.0% sodium hexametaphosphate (HMP); 0.03% quercetin (QC); F+HMP; F+QC; HMP+QC; and F+HMP+QC. Erosive challenges were performed 4×/day for 5 days. Specimens were treated with the respective solutions for one minute, twice a day. Next, dentin loss (profilometry) and integrated hardness area in depth (KHN × µm) were determined. The antiproteolytic potential was assessed by gelatin zymography. Dentin erosion results (log10-transformed) were submitted to one-way ANOVA, followed by Tukey's test. Integrated hardness area in depth data (raw) were submitted to two-way, repeated-measures ANOVA, followed by Holm-Sidak's test (p |
| doi_str_mv | 10.1016/j.archoralbio.2022.105541 |
| format | Article |
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DESIGNRoot dentin blocks (n = 96) were prepared and divided into 8 experimental groups (n = 12/group), according to the solutions to be tested: Placebo; 0.24% sodium fluoride (F); 1.0% sodium hexametaphosphate (HMP); 0.03% quercetin (QC); F+HMP; F+QC; HMP+QC; and F+HMP+QC. Erosive challenges were performed 4×/day for 5 days. Specimens were treated with the respective solutions for one minute, twice a day. Next, dentin loss (profilometry) and integrated hardness area in depth (KHN × µm) were determined. The antiproteolytic potential was assessed by gelatin zymography. Dentin erosion results (log10-transformed) were submitted to one-way ANOVA, followed by Tukey's test. Integrated hardness area in depth data (raw) were submitted to two-way, repeated-measures ANOVA, followed by Holm-Sidak's test (p<0.05). RESULTSDentin erosion was significantly lower for F+HMP+QC than for all other treatments. At the shallowest depths (5-30 µm), blocks treated with F+HMP+QC had the highest integrated hardness area in depth values. All treatments completely inhibited matrix metalloproteinases-2 activity, except for the group QC (77% inhibition). For matrix metalloproteinases-9, all HMP-containing solutions or F+QC promoted total antiproteolytic activity. CONCLUSIONThe association of fluoride, sodium hexametaphosphate, and quercetin must be considered a valuable strategy for novel product formulation for home and professional use, considering its superior protective effects against dentin erosion and its antiproteolytic potential.</description><identifier>ISSN: 0003-9969</identifier><identifier>EISSN: 1879-1506</identifier><identifier>DOI: 10.1016/j.archoralbio.2022.105541</identifier><language>eng</language><ispartof>Archives of oral biology, 2022-11, Vol.143, p.105541-105541, Article 105541</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-5fd222f9199bc33684e103725c1f3b969d50514fd2b33a71e2cbed01ef153dc43</citedby><cites>FETCH-LOGICAL-c345t-5fd222f9199bc33684e103725c1f3b969d50514fd2b33a71e2cbed01ef153dc43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Capalbo, Letícia Cabrera</creatorcontrib><creatorcontrib>Delbem, Alberto Carlos Botazzo</creatorcontrib><creatorcontrib>Dal-Fabbro, Renan</creatorcontrib><creatorcontrib>Inácio, Kelly Karina</creatorcontrib><creatorcontrib>de Oliveira, Rodrigo Cardoso</creatorcontrib><creatorcontrib>Pessan, Juliano Pelim</creatorcontrib><title>Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro</title><title>Archives of oral biology</title><description>OBJECTIVEto investigate the ability of solutions containing sodium hexametaphosphate, fluoride and quercetin, alone or in association, to prevent dentin erosion and to inhibit matrix metalloproteinases -2 and -9 activity using in vitro protocols. DESIGNRoot dentin blocks (n = 96) were prepared and divided into 8 experimental groups (n = 12/group), according to the solutions to be tested: Placebo; 0.24% sodium fluoride (F); 1.0% sodium hexametaphosphate (HMP); 0.03% quercetin (QC); F+HMP; F+QC; HMP+QC; and F+HMP+QC. Erosive challenges were performed 4×/day for 5 days. Specimens were treated with the respective solutions for one minute, twice a day. Next, dentin loss (profilometry) and integrated hardness area in depth (KHN × µm) were determined. The antiproteolytic potential was assessed by gelatin zymography. Dentin erosion results (log10-transformed) were submitted to one-way ANOVA, followed by Tukey's test. Integrated hardness area in depth data (raw) were submitted to two-way, repeated-measures ANOVA, followed by Holm-Sidak's test (p<0.05). RESULTSDentin erosion was significantly lower for F+HMP+QC than for all other treatments. At the shallowest depths (5-30 µm), blocks treated with F+HMP+QC had the highest integrated hardness area in depth values. All treatments completely inhibited matrix metalloproteinases-2 activity, except for the group QC (77% inhibition). For matrix metalloproteinases-9, all HMP-containing solutions or F+QC promoted total antiproteolytic activity. CONCLUSIONThe association of fluoride, sodium hexametaphosphate, and quercetin must be considered a valuable strategy for novel product formulation for home and professional use, considering its superior protective effects against dentin erosion and its antiproteolytic potential.</description><issn>0003-9969</issn><issn>1879-1506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpNkMtOwzAQRS0EEqXwD2bHoil-xEm9RFV5SJXYwNpy7LHiKomL7fL4e1yVBauZO3M1unMQuqVkSQlt7ndLHU0foh46H5aMMFbmQtT0DM3oqpUVFaQ5RzNCCK-kbOQlukppV6RoGjpD48Y5MBkHh1Ow_jDiHr71CFnv-5D2vc6A9WTxxwGigeynBdYpBePLwuIQ8RQy_vK5x244hOgtLHCYsIWpeDHEkHyRpf30OYZrdOH0kODmr87R--Pmbf1cbV-fXtYP28rwWuRKOMsYc5JK2RnOm1UNlPCWCUMd78oTVhBB6-LqONctBWY6sISCo4JbU_M5ujvd3cdQkqesRp8MDIOeIBySYi0VreTtqilWebKakjVFcGof_ajjj6JEHRGrnfqHWB0RqxNi_gt4d3VB</recordid><startdate>202211</startdate><enddate>202211</enddate><creator>Capalbo, Letícia Cabrera</creator><creator>Delbem, Alberto Carlos Botazzo</creator><creator>Dal-Fabbro, Renan</creator><creator>Inácio, Kelly Karina</creator><creator>de Oliveira, Rodrigo Cardoso</creator><creator>Pessan, Juliano Pelim</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202211</creationdate><title>Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro</title><author>Capalbo, Letícia Cabrera ; Delbem, Alberto Carlos Botazzo ; Dal-Fabbro, Renan ; Inácio, Kelly Karina ; de Oliveira, Rodrigo Cardoso ; Pessan, Juliano Pelim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c345t-5fd222f9199bc33684e103725c1f3b969d50514fd2b33a71e2cbed01ef153dc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Capalbo, Letícia Cabrera</creatorcontrib><creatorcontrib>Delbem, Alberto Carlos Botazzo</creatorcontrib><creatorcontrib>Dal-Fabbro, Renan</creatorcontrib><creatorcontrib>Inácio, Kelly Karina</creatorcontrib><creatorcontrib>de Oliveira, Rodrigo Cardoso</creatorcontrib><creatorcontrib>Pessan, Juliano Pelim</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of oral biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Capalbo, Letícia Cabrera</au><au>Delbem, Alberto Carlos Botazzo</au><au>Dal-Fabbro, Renan</au><au>Inácio, Kelly Karina</au><au>de Oliveira, Rodrigo Cardoso</au><au>Pessan, Juliano Pelim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro</atitle><jtitle>Archives of oral biology</jtitle><date>2022-11</date><risdate>2022</risdate><volume>143</volume><spage>105541</spage><epage>105541</epage><pages>105541-105541</pages><artnum>105541</artnum><issn>0003-9969</issn><eissn>1879-1506</eissn><abstract>OBJECTIVEto investigate the ability of solutions containing sodium hexametaphosphate, fluoride and quercetin, alone or in association, to prevent dentin erosion and to inhibit matrix metalloproteinases -2 and -9 activity using in vitro protocols. DESIGNRoot dentin blocks (n = 96) were prepared and divided into 8 experimental groups (n = 12/group), according to the solutions to be tested: Placebo; 0.24% sodium fluoride (F); 1.0% sodium hexametaphosphate (HMP); 0.03% quercetin (QC); F+HMP; F+QC; HMP+QC; and F+HMP+QC. Erosive challenges were performed 4×/day for 5 days. Specimens were treated with the respective solutions for one minute, twice a day. Next, dentin loss (profilometry) and integrated hardness area in depth (KHN × µm) were determined. The antiproteolytic potential was assessed by gelatin zymography. Dentin erosion results (log10-transformed) were submitted to one-way ANOVA, followed by Tukey's test. Integrated hardness area in depth data (raw) were submitted to two-way, repeated-measures ANOVA, followed by Holm-Sidak's test (p<0.05). RESULTSDentin erosion was significantly lower for F+HMP+QC than for all other treatments. At the shallowest depths (5-30 µm), blocks treated with F+HMP+QC had the highest integrated hardness area in depth values. All treatments completely inhibited matrix metalloproteinases-2 activity, except for the group QC (77% inhibition). For matrix metalloproteinases-9, all HMP-containing solutions or F+QC promoted total antiproteolytic activity. CONCLUSIONThe association of fluoride, sodium hexametaphosphate, and quercetin must be considered a valuable strategy for novel product formulation for home and professional use, considering its superior protective effects against dentin erosion and its antiproteolytic potential.</abstract><doi>10.1016/j.archoralbio.2022.105541</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| title | Effect of sodium hexametaphosphate and quercetin, associated or not with fluoride, on dentin erosion in vitro |
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