Karyotyping of commercial cultivars of melon (Cucumis melo L.)
Background This study on cultivars of melon ( Cucumis melo L.) marketed in Brazil was conducted to obtain information to be used in breeding programs of this species. Little is known about the karyotype variability among C. melo L. cultivars targeted at the consumer market. The objective of the pres...
Gespeichert in:
| Veröffentlicht in: | Molecular biology reports 2022-11, Vol.49 (11), p.10279-10292 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 10292 |
|---|---|
| container_issue | 11 |
| container_start_page | 10279 |
| container_title | Molecular biology reports |
| container_volume | 49 |
| creator | Santos, Matusalem Campos Souza, Margarete Magalhães de Melo, Cláusio Antônio Ferreira Silva, Gonçalo Santos |
| description | Background
This study on cultivars of melon (
Cucumis melo
L.) marketed in Brazil was conducted to obtain information to be used in breeding programs of this species. Little is known about the karyotype variability among
C. melo
L. cultivars targeted at the consumer market. The objective of the present study was to verify the karyotype variability in eight commercial melon cultivars used in the Brazilian market.
Methods and results
Slides were stained with 2% Giemsa and assembled with Neomount to perform chromosomal morphometry. GC-rich heterochromatin was observed by CMA
3
/DAPI staining. 5 S rDNA, centromeric satellite DNA (SatDNA), and telomeric sites were visualized using fluorescence
in situ
hybridization. All images were captured on an Olympus BX41 microscope equipped with a 5 M Olympus DP25 digital camera and DP2-BSW software. The cultivars showed symmetrical karyotypes with significant differences in total chromosome length and average chromosome size. Heterochromatic CMA
3
+
blocks were observed in terminal regions related to satellites (secondary constrictions), as well as in centromeric and pericentromeric regions. A single chromosomal pair of 5 S rDNA sites was observed in all cultivars, but at distinct locations. Centromeric satellite sequences, tested for the first time in melon, revealed only centromeric sites. Telomeric sites were observed in all the chromosomes of the cultivars.
Conclusions
Karyotype variation was observed in cultivars of melon, which were analyzed for chromosomal morphology and localization of GC-rich heterochromatin, as well centromeric SatDNA, rDNA, and telomeric chromosomal markers. Hence, these cultivars can be used in future breeding programs. |
| doi_str_mv | 10.1007/s11033-022-07520-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2714059769</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2714059769</sourcerecordid><originalsourceid>FETCH-LOGICAL-c282t-5b32eb3438c4842f7a488f5c606c73b8f2124491388b126a007534721ba739493</originalsourceid><addsrcrecordid>eNp9kE1LAzEQhoMoWKt_wNOCl3pInXxtshdBSv3Aghc9h2zIli27m5rsCu2vN-0KggdPwwzPO8w8CF0TmBMAeRcJAcYwUIpBCgp4f4ImREiGeSHVKZoAA4K5EuQcXcS4AQBOpJig-1cTdr7fbetunfkqs75tXbC1aTI7NH39ZUI8zFvX-C6bLQY7tHU8ttlqfnuJzirTRHf1U6fo43H5vnjGq7enl8XDCluqaI9FyagrGWfKcsVpJQ1XqhI2h9xKVqqKEsp5QZhSJaG5SS8JxiUlpZGs4AWbotm4dxv85-Bir9MV1jWN6ZwfoqaScBCFzA_ozR9044fQpesSlSxIpRRJFB0pG3yMwVV6G-o2udAE9EGpHpXqpFQflep9CrExFBPcrV34Xf1P6huwrnZN</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2730178881</pqid></control><display><type>article</type><title>Karyotyping of commercial cultivars of melon (Cucumis melo L.)</title><source>SpringerLink Journals - AutoHoldings</source><creator>Santos, Matusalem Campos ; Souza, Margarete Magalhães ; de Melo, Cláusio Antônio Ferreira ; Silva, Gonçalo Santos</creator><creatorcontrib>Santos, Matusalem Campos ; Souza, Margarete Magalhães ; de Melo, Cláusio Antônio Ferreira ; Silva, Gonçalo Santos</creatorcontrib><description>Background
This study on cultivars of melon (
Cucumis melo
L.) marketed in Brazil was conducted to obtain information to be used in breeding programs of this species. Little is known about the karyotype variability among
C. melo
L. cultivars targeted at the consumer market. The objective of the present study was to verify the karyotype variability in eight commercial melon cultivars used in the Brazilian market.
Methods and results
Slides were stained with 2% Giemsa and assembled with Neomount to perform chromosomal morphometry. GC-rich heterochromatin was observed by CMA
3
/DAPI staining. 5 S rDNA, centromeric satellite DNA (SatDNA), and telomeric sites were visualized using fluorescence
in situ
hybridization. All images were captured on an Olympus BX41 microscope equipped with a 5 M Olympus DP25 digital camera and DP2-BSW software. The cultivars showed symmetrical karyotypes with significant differences in total chromosome length and average chromosome size. Heterochromatic CMA
3
+
blocks were observed in terminal regions related to satellites (secondary constrictions), as well as in centromeric and pericentromeric regions. A single chromosomal pair of 5 S rDNA sites was observed in all cultivars, but at distinct locations. Centromeric satellite sequences, tested for the first time in melon, revealed only centromeric sites. Telomeric sites were observed in all the chromosomes of the cultivars.
Conclusions
Karyotype variation was observed in cultivars of melon, which were analyzed for chromosomal morphology and localization of GC-rich heterochromatin, as well centromeric SatDNA, rDNA, and telomeric chromosomal markers. Hence, these cultivars can be used in future breeding programs.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-022-07520-z</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Chromosomes ; Cucumis melo ; Cultivars ; Fluorescence in situ hybridization ; Heterochromatin ; Histology ; Karyotypes ; Life Sciences ; Localization ; Morphology ; Morphometry ; Original Article ; Plant breeding ; Satellite DNA ; Satellites</subject><ispartof>Molecular biology reports, 2022-11, Vol.49 (11), p.10279-10292</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022</rights><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-5b32eb3438c4842f7a488f5c606c73b8f2124491388b126a007534721ba739493</citedby><cites>FETCH-LOGICAL-c282t-5b32eb3438c4842f7a488f5c606c73b8f2124491388b126a007534721ba739493</cites><orcidid>0000-0003-0292-7988</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-022-07520-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-022-07520-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids></links><search><creatorcontrib>Santos, Matusalem Campos</creatorcontrib><creatorcontrib>Souza, Margarete Magalhães</creatorcontrib><creatorcontrib>de Melo, Cláusio Antônio Ferreira</creatorcontrib><creatorcontrib>Silva, Gonçalo Santos</creatorcontrib><title>Karyotyping of commercial cultivars of melon (Cucumis melo L.)</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><description>Background
This study on cultivars of melon (
Cucumis melo
L.) marketed in Brazil was conducted to obtain information to be used in breeding programs of this species. Little is known about the karyotype variability among
C. melo
L. cultivars targeted at the consumer market. The objective of the present study was to verify the karyotype variability in eight commercial melon cultivars used in the Brazilian market.
Methods and results
Slides were stained with 2% Giemsa and assembled with Neomount to perform chromosomal morphometry. GC-rich heterochromatin was observed by CMA
3
/DAPI staining. 5 S rDNA, centromeric satellite DNA (SatDNA), and telomeric sites were visualized using fluorescence
in situ
hybridization. All images were captured on an Olympus BX41 microscope equipped with a 5 M Olympus DP25 digital camera and DP2-BSW software. The cultivars showed symmetrical karyotypes with significant differences in total chromosome length and average chromosome size. Heterochromatic CMA
3
+
blocks were observed in terminal regions related to satellites (secondary constrictions), as well as in centromeric and pericentromeric regions. A single chromosomal pair of 5 S rDNA sites was observed in all cultivars, but at distinct locations. Centromeric satellite sequences, tested for the first time in melon, revealed only centromeric sites. Telomeric sites were observed in all the chromosomes of the cultivars.
Conclusions
Karyotype variation was observed in cultivars of melon, which were analyzed for chromosomal morphology and localization of GC-rich heterochromatin, as well centromeric SatDNA, rDNA, and telomeric chromosomal markers. Hence, these cultivars can be used in future breeding programs.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Chromosomes</subject><subject>Cucumis melo</subject><subject>Cultivars</subject><subject>Fluorescence in situ hybridization</subject><subject>Heterochromatin</subject><subject>Histology</subject><subject>Karyotypes</subject><subject>Life Sciences</subject><subject>Localization</subject><subject>Morphology</subject><subject>Morphometry</subject><subject>Original Article</subject><subject>Plant breeding</subject><subject>Satellite DNA</subject><subject>Satellites</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE1LAzEQhoMoWKt_wNOCl3pInXxtshdBSv3Aghc9h2zIli27m5rsCu2vN-0KggdPwwzPO8w8CF0TmBMAeRcJAcYwUIpBCgp4f4ImREiGeSHVKZoAA4K5EuQcXcS4AQBOpJig-1cTdr7fbetunfkqs75tXbC1aTI7NH39ZUI8zFvX-C6bLQY7tHU8ttlqfnuJzirTRHf1U6fo43H5vnjGq7enl8XDCluqaI9FyagrGWfKcsVpJQ1XqhI2h9xKVqqKEsp5QZhSJaG5SS8JxiUlpZGs4AWbotm4dxv85-Bir9MV1jWN6ZwfoqaScBCFzA_ozR9044fQpesSlSxIpRRJFB0pG3yMwVV6G-o2udAE9EGpHpXqpFQflep9CrExFBPcrV34Xf1P6huwrnZN</recordid><startdate>20221101</startdate><enddate>20221101</enddate><creator>Santos, Matusalem Campos</creator><creator>Souza, Margarete Magalhães</creator><creator>de Melo, Cláusio Antônio Ferreira</creator><creator>Silva, Gonçalo Santos</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0292-7988</orcidid></search><sort><creationdate>20221101</creationdate><title>Karyotyping of commercial cultivars of melon (Cucumis melo L.)</title><author>Santos, Matusalem Campos ; Souza, Margarete Magalhães ; de Melo, Cláusio Antônio Ferreira ; Silva, Gonçalo Santos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-5b32eb3438c4842f7a488f5c606c73b8f2124491388b126a007534721ba739493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Chromosomes</topic><topic>Cucumis melo</topic><topic>Cultivars</topic><topic>Fluorescence in situ hybridization</topic><topic>Heterochromatin</topic><topic>Histology</topic><topic>Karyotypes</topic><topic>Life Sciences</topic><topic>Localization</topic><topic>Morphology</topic><topic>Morphometry</topic><topic>Original Article</topic><topic>Plant breeding</topic><topic>Satellite DNA</topic><topic>Satellites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santos, Matusalem Campos</creatorcontrib><creatorcontrib>Souza, Margarete Magalhães</creatorcontrib><creatorcontrib>de Melo, Cláusio Antônio Ferreira</creatorcontrib><creatorcontrib>Silva, Gonçalo Santos</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santos, Matusalem Campos</au><au>Souza, Margarete Magalhães</au><au>de Melo, Cláusio Antônio Ferreira</au><au>Silva, Gonçalo Santos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Karyotyping of commercial cultivars of melon (Cucumis melo L.)</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><date>2022-11-01</date><risdate>2022</risdate><volume>49</volume><issue>11</issue><spage>10279</spage><epage>10292</epage><pages>10279-10292</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
This study on cultivars of melon (
Cucumis melo
L.) marketed in Brazil was conducted to obtain information to be used in breeding programs of this species. Little is known about the karyotype variability among
C. melo
L. cultivars targeted at the consumer market. The objective of the present study was to verify the karyotype variability in eight commercial melon cultivars used in the Brazilian market.
Methods and results
Slides were stained with 2% Giemsa and assembled with Neomount to perform chromosomal morphometry. GC-rich heterochromatin was observed by CMA
3
/DAPI staining. 5 S rDNA, centromeric satellite DNA (SatDNA), and telomeric sites were visualized using fluorescence
in situ
hybridization. All images were captured on an Olympus BX41 microscope equipped with a 5 M Olympus DP25 digital camera and DP2-BSW software. The cultivars showed symmetrical karyotypes with significant differences in total chromosome length and average chromosome size. Heterochromatic CMA
3
+
blocks were observed in terminal regions related to satellites (secondary constrictions), as well as in centromeric and pericentromeric regions. A single chromosomal pair of 5 S rDNA sites was observed in all cultivars, but at distinct locations. Centromeric satellite sequences, tested for the first time in melon, revealed only centromeric sites. Telomeric sites were observed in all the chromosomes of the cultivars.
Conclusions
Karyotype variation was observed in cultivars of melon, which were analyzed for chromosomal morphology and localization of GC-rich heterochromatin, as well centromeric SatDNA, rDNA, and telomeric chromosomal markers. Hence, these cultivars can be used in future breeding programs.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11033-022-07520-z</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0003-0292-7988</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4851 |
| ispartof | Molecular biology reports, 2022-11, Vol.49 (11), p.10279-10292 |
| issn | 0301-4851 1573-4978 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2714059769 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Chromosomes Cucumis melo Cultivars Fluorescence in situ hybridization Heterochromatin Histology Karyotypes Life Sciences Localization Morphology Morphometry Original Article Plant breeding Satellite DNA Satellites |
| title | Karyotyping of commercial cultivars of melon (Cucumis melo L.) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T07%3A36%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Karyotyping%20of%20commercial%20cultivars%20of%20melon%20(Cucumis%20melo%20L.)&rft.jtitle=Molecular%20biology%20reports&rft.au=Santos,%20Matusalem%20Campos&rft.date=2022-11-01&rft.volume=49&rft.issue=11&rft.spage=10279&rft.epage=10292&rft.pages=10279-10292&rft.issn=0301-4851&rft.eissn=1573-4978&rft_id=info:doi/10.1007/s11033-022-07520-z&rft_dat=%3Cproquest_cross%3E2714059769%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2730178881&rft_id=info:pmid/&rfr_iscdi=true |