Characterization of neutral lipases revealed the tissue‐specific triacylglycerol hydrolytic activity in Nilaparvata lugens
The lipid metabolism plays an essential role in the development and reproduction of insects, and lipases are important enzymes in lipid metabolism. In Nilaparvata lugens, an important insect pest on rice, triacylglycerol hydrolytic activities were different among tissues, with high activity in integ...
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| Veröffentlicht in: | Insect science 2023-06, Vol.30 (3), p.693-704 |
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| creator | Zhang, Hui‐Hui Yang, Bao‐Jun Wu, Yong Gao, Hao‐Li Lin, Xu‐Min Zou, Jian‐Zheng Liu, Ze‐Wen |
| description | The lipid metabolism plays an essential role in the development and reproduction of insects, and lipases are important enzymes in lipid metabolism. In Nilaparvata lugens, an important insect pest on rice, triacylglycerol hydrolytic activities were different among tissues, with high activity in integument, ovary, and fat body, but low activity in intestine. To figure out the tissue‐specific triacylglycerol hydrolytic activity, we identified 43 lipases in N. lugens. Of these 43 lipases, 23 belonged to neutral lipases, so this group was selected to perform further experiments on triacylglycerol hydrolysis. The complete motifs of catalytic triads, β9 loop, and lid motif, are required for the triacylglycerol hydrolytic activity in neutral lipases, which were found in some neutral lipases with high gene expression levels in integument and ovary, but not in intestine. The recombinant proteins of 3 neutral lipases with or without 3 complete motifs were obtained, and the activity determination confirmed the importance of 3 motifs. Silencing XM_022331066.1, which is highly expressed in ovary and with 3 complete motifs, significantly decreased the egg production and hatchability of N. lugens, partially through decline of the lipid metabolism. In summary, at least one‐third of important motifs were incomplete in all neutral lipases with high gene expression in intestine, which could partially explain why the lipase activity in intestine was much lower than that in other tissues. The low activity to hydrolyze triacylglycerol in N. lugens intestine might be associated with its food resource and nutrient components, and the ovary‐specific neutral lipases were important for N. lugens reproduction.
Forty‐three lipases in Nilaparvata lugens were identified, with half of them belonging to neutral lipases. The neutral lipases in N. lugens intestine lost triacylglycerol hydrolytic activity and ovary‐specific neutral lipases played important roles in lipid metabolism and the reproduction of N. lugens. |
| doi_str_mv | 10.1111/1744-7917.13118 |
| format | Article |
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Forty‐three lipases in Nilaparvata lugens were identified, with half of them belonging to neutral lipases. The neutral lipases in N. lugens intestine lost triacylglycerol hydrolytic activity and ovary‐specific neutral lipases played important roles in lipid metabolism and the reproduction of N. lugens.</description><identifier>ISSN: 1672-9609</identifier><identifier>EISSN: 1744-7917</identifier><identifier>DOI: 10.1111/1744-7917.13118</identifier><identifier>PMID: 36093889</identifier><language>eng</language><publisher>Australia: Wiley Subscription Services, Inc</publisher><subject>Animals ; Egg production ; Fat body ; Female ; Gene expression ; Hatchability ; Hemiptera ; Hydrolysis ; Insecta - metabolism ; Insects ; Integument ; Intestine ; Lipase - genetics ; Lipase - metabolism ; lipases ; Lipid metabolism ; Lipids ; Metabolism ; neutral lipases ; Nilaparvata lugens ; Ovaries ; reproduction ; Tissues ; triacylglycerol hydrolytic activity ; Triglycerides ; Triglycerides - metabolism</subject><ispartof>Insect science, 2023-06, Vol.30 (3), p.693-704</ispartof><rights>2022 Institute of Zoology, Chinese Academy of Sciences.</rights><rights>2023 Institute of Zoology, Chinese Academy of Sciences.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3718-c9ed8b258fbd615cb9b7893afe015dffafbdd9c93e897445e9d3d484fe981323</citedby><cites>FETCH-LOGICAL-c3718-c9ed8b258fbd615cb9b7893afe015dffafbdd9c93e897445e9d3d484fe981323</cites><orcidid>0000-0003-1084-9449</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1744-7917.13118$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1744-7917.13118$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36093889$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Hui‐Hui</creatorcontrib><creatorcontrib>Yang, Bao‐Jun</creatorcontrib><creatorcontrib>Wu, Yong</creatorcontrib><creatorcontrib>Gao, Hao‐Li</creatorcontrib><creatorcontrib>Lin, Xu‐Min</creatorcontrib><creatorcontrib>Zou, Jian‐Zheng</creatorcontrib><creatorcontrib>Liu, Ze‐Wen</creatorcontrib><title>Characterization of neutral lipases revealed the tissue‐specific triacylglycerol hydrolytic activity in Nilaparvata lugens</title><title>Insect science</title><addtitle>Insect Sci</addtitle><description>The lipid metabolism plays an essential role in the development and reproduction of insects, and lipases are important enzymes in lipid metabolism. In Nilaparvata lugens, an important insect pest on rice, triacylglycerol hydrolytic activities were different among tissues, with high activity in integument, ovary, and fat body, but low activity in intestine. To figure out the tissue‐specific triacylglycerol hydrolytic activity, we identified 43 lipases in N. lugens. Of these 43 lipases, 23 belonged to neutral lipases, so this group was selected to perform further experiments on triacylglycerol hydrolysis. The complete motifs of catalytic triads, β9 loop, and lid motif, are required for the triacylglycerol hydrolytic activity in neutral lipases, which were found in some neutral lipases with high gene expression levels in integument and ovary, but not in intestine. The recombinant proteins of 3 neutral lipases with or without 3 complete motifs were obtained, and the activity determination confirmed the importance of 3 motifs. Silencing XM_022331066.1, which is highly expressed in ovary and with 3 complete motifs, significantly decreased the egg production and hatchability of N. lugens, partially through decline of the lipid metabolism. In summary, at least one‐third of important motifs were incomplete in all neutral lipases with high gene expression in intestine, which could partially explain why the lipase activity in intestine was much lower than that in other tissues. The low activity to hydrolyze triacylglycerol in N. lugens intestine might be associated with its food resource and nutrient components, and the ovary‐specific neutral lipases were important for N. lugens reproduction.
Forty‐three lipases in Nilaparvata lugens were identified, with half of them belonging to neutral lipases. The neutral lipases in N. lugens intestine lost triacylglycerol hydrolytic activity and ovary‐specific neutral lipases played important roles in lipid metabolism and the reproduction of N. lugens.</description><subject>Animals</subject><subject>Egg production</subject><subject>Fat body</subject><subject>Female</subject><subject>Gene expression</subject><subject>Hatchability</subject><subject>Hemiptera</subject><subject>Hydrolysis</subject><subject>Insecta - metabolism</subject><subject>Insects</subject><subject>Integument</subject><subject>Intestine</subject><subject>Lipase - genetics</subject><subject>Lipase - metabolism</subject><subject>lipases</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Metabolism</subject><subject>neutral lipases</subject><subject>Nilaparvata lugens</subject><subject>Ovaries</subject><subject>reproduction</subject><subject>Tissues</subject><subject>triacylglycerol hydrolytic activity</subject><subject>Triglycerides</subject><subject>Triglycerides - metabolism</subject><issn>1672-9609</issn><issn>1744-7917</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFPHCEYhklTU6167q0h6aWX0QF2ZuDYbFo1MXpw74SBDxfDzkyB2WaMB3-Cv9FfIttdPXiRy0deHt5AHoS-kfKE5HVKmtmsaARpTggjhH9CB2_J57yvG1qIuhT76GuMd2XJBBX0C9pnOWOciwP0MF-qoHSC4O5Vcn2He4s7GFNQHns3qAgRB1iD8mBwWgJOLsYRnh-f4gDaWadxCk7pyd_6SUPoPV5OJo8p5aPc7NYuTdh1-Mp5NaiwVklhP95CF4_QnlU-wvFuHqLFn9-L-XlxeX12Mf91WWjWEF5oAYa3tOK2NTWpdCvahgumLJSkMtaqnBuhBQMu8u8rEIaZGZ9ZEJwwyg7Rz23tEPq_I8QkVy5q8F510I9R0oYwRmhT1xn98Q6968fQ5cdJymlFayEqkqnTLaVDH2MAK4fgVipMkpRy40VuLMiNBfnfS77xfdc7tiswb_yriAxUW-Cf8zB91Ccvrm62xS9BKpvz</recordid><startdate>202306</startdate><enddate>202306</enddate><creator>Zhang, Hui‐Hui</creator><creator>Yang, Bao‐Jun</creator><creator>Wu, Yong</creator><creator>Gao, Hao‐Li</creator><creator>Lin, Xu‐Min</creator><creator>Zou, Jian‐Zheng</creator><creator>Liu, Ze‐Wen</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1084-9449</orcidid></search><sort><creationdate>202306</creationdate><title>Characterization of neutral lipases revealed the tissue‐specific triacylglycerol hydrolytic activity in Nilaparvata lugens</title><author>Zhang, Hui‐Hui ; Yang, Bao‐Jun ; Wu, Yong ; Gao, Hao‐Li ; Lin, Xu‐Min ; Zou, Jian‐Zheng ; Liu, Ze‐Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3718-c9ed8b258fbd615cb9b7893afe015dffafbdd9c93e897445e9d3d484fe981323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Egg production</topic><topic>Fat body</topic><topic>Female</topic><topic>Gene expression</topic><topic>Hatchability</topic><topic>Hemiptera</topic><topic>Hydrolysis</topic><topic>Insecta - metabolism</topic><topic>Insects</topic><topic>Integument</topic><topic>Intestine</topic><topic>Lipase - genetics</topic><topic>Lipase - metabolism</topic><topic>lipases</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Metabolism</topic><topic>neutral lipases</topic><topic>Nilaparvata lugens</topic><topic>Ovaries</topic><topic>reproduction</topic><topic>Tissues</topic><topic>triacylglycerol hydrolytic activity</topic><topic>Triglycerides</topic><topic>Triglycerides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Hui‐Hui</creatorcontrib><creatorcontrib>Yang, Bao‐Jun</creatorcontrib><creatorcontrib>Wu, Yong</creatorcontrib><creatorcontrib>Gao, Hao‐Li</creatorcontrib><creatorcontrib>Lin, Xu‐Min</creatorcontrib><creatorcontrib>Zou, Jian‐Zheng</creatorcontrib><creatorcontrib>Liu, Ze‐Wen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Hui‐Hui</au><au>Yang, Bao‐Jun</au><au>Wu, Yong</au><au>Gao, Hao‐Li</au><au>Lin, Xu‐Min</au><au>Zou, Jian‐Zheng</au><au>Liu, Ze‐Wen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of neutral lipases revealed the tissue‐specific triacylglycerol hydrolytic activity in Nilaparvata lugens</atitle><jtitle>Insect science</jtitle><addtitle>Insect Sci</addtitle><date>2023-06</date><risdate>2023</risdate><volume>30</volume><issue>3</issue><spage>693</spage><epage>704</epage><pages>693-704</pages><issn>1672-9609</issn><eissn>1744-7917</eissn><abstract>The lipid metabolism plays an essential role in the development and reproduction of insects, and lipases are important enzymes in lipid metabolism. In Nilaparvata lugens, an important insect pest on rice, triacylglycerol hydrolytic activities were different among tissues, with high activity in integument, ovary, and fat body, but low activity in intestine. To figure out the tissue‐specific triacylglycerol hydrolytic activity, we identified 43 lipases in N. lugens. Of these 43 lipases, 23 belonged to neutral lipases, so this group was selected to perform further experiments on triacylglycerol hydrolysis. The complete motifs of catalytic triads, β9 loop, and lid motif, are required for the triacylglycerol hydrolytic activity in neutral lipases, which were found in some neutral lipases with high gene expression levels in integument and ovary, but not in intestine. The recombinant proteins of 3 neutral lipases with or without 3 complete motifs were obtained, and the activity determination confirmed the importance of 3 motifs. Silencing XM_022331066.1, which is highly expressed in ovary and with 3 complete motifs, significantly decreased the egg production and hatchability of N. lugens, partially through decline of the lipid metabolism. In summary, at least one‐third of important motifs were incomplete in all neutral lipases with high gene expression in intestine, which could partially explain why the lipase activity in intestine was much lower than that in other tissues. The low activity to hydrolyze triacylglycerol in N. lugens intestine might be associated with its food resource and nutrient components, and the ovary‐specific neutral lipases were important for N. lugens reproduction.
Forty‐three lipases in Nilaparvata lugens were identified, with half of them belonging to neutral lipases. The neutral lipases in N. lugens intestine lost triacylglycerol hydrolytic activity and ovary‐specific neutral lipases played important roles in lipid metabolism and the reproduction of N. lugens.</abstract><cop>Australia</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36093889</pmid><doi>10.1111/1744-7917.13118</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-1084-9449</orcidid></addata></record> |
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| subjects | Animals Egg production Fat body Female Gene expression Hatchability Hemiptera Hydrolysis Insecta - metabolism Insects Integument Intestine Lipase - genetics Lipase - metabolism lipases Lipid metabolism Lipids Metabolism neutral lipases Nilaparvata lugens Ovaries reproduction Tissues triacylglycerol hydrolytic activity Triglycerides Triglycerides - metabolism |
| title | Characterization of neutral lipases revealed the tissue‐specific triacylglycerol hydrolytic activity in Nilaparvata lugens |
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