Genotypic and phenotypic comparison of clinical and environmental Acinetobacter baumannii strains
The genotypic and phenotypic characteristics and antibiotic resistance (antibiogram) profiles of clinical (n = 13) and environmental (n = 7) Acinetobacter baumannii isolates were compared. Based on the Repetitive Extragenic Palindromic Sequence-based PCR (REP-PCR) analysis, the clinical and environm...
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| Veröffentlicht in: | Microbial pathogenesis 2022-11, Vol.172, p.105749-105749, Article 105749 |
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| creator | Havenga, Benjamin Reyneke, Brandon Ndlovu, Thando Khan, Wesaal |
| description | The genotypic and phenotypic characteristics and antibiotic resistance (antibiogram) profiles of clinical (n = 13) and environmental (n = 7) Acinetobacter baumannii isolates were compared. Based on the Repetitive Extragenic Palindromic Sequence-based PCR (REP-PCR) analysis, the clinical and environmental A. baumannii isolates shared low genetic relatedness (∼60%). Multilocus sequence typing (MLST, Oxford scheme) indicated that the clinical A. baumannii were assigned to three sequence types (ST231, ST945 and ST848), while the environmental A. baumannii (excluding AB 14) were categorised into the novel ST2520. The majority of the clinical (excluding AB 5, CAB 11, CAC 37) and environmental (excluding AB 14 and AB 16) A. baumannii strains were then capable of phase variation with both the translucent (71.4%; 15/21) and opaque (95.2%; 20/21) colony phenotypes detected. The clinical isolates however, exhibited significantly (p |
| doi_str_mv | 10.1016/j.micpath.2022.105749 |
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[Display omitted]
•Low genetic relatedness observed between clinical and environmental A. baumannii isolates.•MLST (Oxford scheme) assigned ST231, ST945 and ST848 to the clinical A. baumannii.•Environmental A. baumannii were categorised (MLST) into the novel ST2520 sequence type.•Clinical A. baumannii exhibited increased biofilm formation and antibiotic resistance.•Colistin resistance only detected amongst five clinical A. baumannii isolates.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2022.105749</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Acinetobacter baumannii ; Antibiotic resistance ; Clinical ; Environmental ; MLST ; ST2520</subject><ispartof>Microbial pathogenesis, 2022-11, Vol.172, p.105749-105749, Article 105749</ispartof><rights>2022 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-19e5de54cb367ecb30bb061c1a57b64d2cfa07053d389ce9c2d7f266991cf173</citedby><cites>FETCH-LOGICAL-c272t-19e5de54cb367ecb30bb061c1a57b64d2cfa07053d389ce9c2d7f266991cf173</cites><orcidid>0000-0003-1559-9485</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2022.105749$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids></links><search><creatorcontrib>Havenga, Benjamin</creatorcontrib><creatorcontrib>Reyneke, Brandon</creatorcontrib><creatorcontrib>Ndlovu, Thando</creatorcontrib><creatorcontrib>Khan, Wesaal</creatorcontrib><title>Genotypic and phenotypic comparison of clinical and environmental Acinetobacter baumannii strains</title><title>Microbial pathogenesis</title><description>The genotypic and phenotypic characteristics and antibiotic resistance (antibiogram) profiles of clinical (n = 13) and environmental (n = 7) Acinetobacter baumannii isolates were compared. Based on the Repetitive Extragenic Palindromic Sequence-based PCR (REP-PCR) analysis, the clinical and environmental A. baumannii isolates shared low genetic relatedness (∼60%). Multilocus sequence typing (MLST, Oxford scheme) indicated that the clinical A. baumannii were assigned to three sequence types (ST231, ST945 and ST848), while the environmental A. baumannii (excluding AB 14) were categorised into the novel ST2520. The majority of the clinical (excluding AB 5, CAB 11, CAC 37) and environmental (excluding AB 14 and AB 16) A. baumannii strains were then capable of phase variation with both the translucent (71.4%; 15/21) and opaque (95.2%; 20/21) colony phenotypes detected. The clinical isolates however, exhibited significantly (p < 0.05) higher biofilm formation capabilities (OD570: 2.094 ± 0.497). Moreover, the clinical isolates exhibited significantly (p < 0.05) higher resistance to first line antibiotics, with 92.3% (12/13) characterised as extensively drug resistant (XDR), whereas environmental A. baumannii exhibited increased antibiotic susceptibility with only 57.1% (4/7) characterised as multidrug resistant (MDR). The environmental isolate AB 14 was however, characterised as XDR. In addition, only five clinical A. baumannii isolates exhibited colistin resistance (38.5%; 5/13). The current study highlighted the differences in the genotypic, phenotypic, and antibiotic resistance profiles of clinical and environmental A. baumannii. Moreover, the environmental strains were assigned to the novel ST2520, which substantiates the existence of this opportunistic pathogen in extra-hospital reservoirs.
[Display omitted]
•Low genetic relatedness observed between clinical and environmental A. baumannii isolates.•MLST (Oxford scheme) assigned ST231, ST945 and ST848 to the clinical A. baumannii.•Environmental A. baumannii were categorised (MLST) into the novel ST2520 sequence type.•Clinical A. baumannii exhibited increased biofilm formation and antibiotic resistance.•Colistin resistance only detected amongst five clinical A. baumannii isolates.</description><subject>Acinetobacter baumannii</subject><subject>Antibiotic resistance</subject><subject>Clinical</subject><subject>Environmental</subject><subject>MLST</subject><subject>ST2520</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkM1LxDAQxYMouH78CUKPXrpO0o80J5FFV2HBi_eQTqdsljapSXbB_96uK169zDCP9x7Mj7E7DksOvH7YLUeLk0nbpQAhZq2SpTpjCw6qzrmA5pwtoGlEXgKHS3YV4w4AVFmoBTNrcj59TRYz47ps2v6d6MfJBBu9y3yf4WCdRTP8uMgdbPBuJJdm5Qmto-Rbg4lC1pr9aJyzNospGOviDbvozRDp9ndfs4-X54_Va755X7-tnjY5CilSzhVVHVUltkUtaZ7QtlBz5KaSbV12AnsDEqqiKxqFpFB0shd1rRTHnsvimt2faqfgP_cUkx5tRBoG48jvoxaSi6YqpSpma3WyYvAxBur1FOxowpfmoI9E9U7_EtVHovpEdM49nnI0v3GwFHRESw6ps4Ew6c7bfxq-AU8thBY</recordid><startdate>202211</startdate><enddate>202211</enddate><creator>Havenga, Benjamin</creator><creator>Reyneke, Brandon</creator><creator>Ndlovu, Thando</creator><creator>Khan, Wesaal</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1559-9485</orcidid></search><sort><creationdate>202211</creationdate><title>Genotypic and phenotypic comparison of clinical and environmental Acinetobacter baumannii strains</title><author>Havenga, Benjamin ; Reyneke, Brandon ; Ndlovu, Thando ; Khan, Wesaal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-19e5de54cb367ecb30bb061c1a57b64d2cfa07053d389ce9c2d7f266991cf173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acinetobacter baumannii</topic><topic>Antibiotic resistance</topic><topic>Clinical</topic><topic>Environmental</topic><topic>MLST</topic><topic>ST2520</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Havenga, Benjamin</creatorcontrib><creatorcontrib>Reyneke, Brandon</creatorcontrib><creatorcontrib>Ndlovu, Thando</creatorcontrib><creatorcontrib>Khan, Wesaal</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Havenga, Benjamin</au><au>Reyneke, Brandon</au><au>Ndlovu, Thando</au><au>Khan, Wesaal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotypic and phenotypic comparison of clinical and environmental Acinetobacter baumannii strains</atitle><jtitle>Microbial pathogenesis</jtitle><date>2022-11</date><risdate>2022</risdate><volume>172</volume><spage>105749</spage><epage>105749</epage><pages>105749-105749</pages><artnum>105749</artnum><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>The genotypic and phenotypic characteristics and antibiotic resistance (antibiogram) profiles of clinical (n = 13) and environmental (n = 7) Acinetobacter baumannii isolates were compared. Based on the Repetitive Extragenic Palindromic Sequence-based PCR (REP-PCR) analysis, the clinical and environmental A. baumannii isolates shared low genetic relatedness (∼60%). Multilocus sequence typing (MLST, Oxford scheme) indicated that the clinical A. baumannii were assigned to three sequence types (ST231, ST945 and ST848), while the environmental A. baumannii (excluding AB 14) were categorised into the novel ST2520. The majority of the clinical (excluding AB 5, CAB 11, CAC 37) and environmental (excluding AB 14 and AB 16) A. baumannii strains were then capable of phase variation with both the translucent (71.4%; 15/21) and opaque (95.2%; 20/21) colony phenotypes detected. The clinical isolates however, exhibited significantly (p < 0.05) higher biofilm formation capabilities (OD570: 2.094 ± 0.497). Moreover, the clinical isolates exhibited significantly (p < 0.05) higher resistance to first line antibiotics, with 92.3% (12/13) characterised as extensively drug resistant (XDR), whereas environmental A. baumannii exhibited increased antibiotic susceptibility with only 57.1% (4/7) characterised as multidrug resistant (MDR). The environmental isolate AB 14 was however, characterised as XDR. In addition, only five clinical A. baumannii isolates exhibited colistin resistance (38.5%; 5/13). The current study highlighted the differences in the genotypic, phenotypic, and antibiotic resistance profiles of clinical and environmental A. baumannii. Moreover, the environmental strains were assigned to the novel ST2520, which substantiates the existence of this opportunistic pathogen in extra-hospital reservoirs.
[Display omitted]
•Low genetic relatedness observed between clinical and environmental A. baumannii isolates.•MLST (Oxford scheme) assigned ST231, ST945 and ST848 to the clinical A. baumannii.•Environmental A. baumannii were categorised (MLST) into the novel ST2520 sequence type.•Clinical A. baumannii exhibited increased biofilm formation and antibiotic resistance.•Colistin resistance only detected amongst five clinical A. baumannii isolates.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.micpath.2022.105749</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1559-9485</orcidid></addata></record> |
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| subjects | Acinetobacter baumannii Antibiotic resistance Clinical Environmental MLST ST2520 |
| title | Genotypic and phenotypic comparison of clinical and environmental Acinetobacter baumannii strains |
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