Developmental light deprivation transiently reduces the expression of vGluT2 and GluN2B in the rat ventral suprachiasmatic nucleus
The suprachiasmatic nucleus (SCN) is the most important circadian clock in mammals. The SCN synchronizes to environmental light via the retinohypothalamic tract (RHT), which is an axon cluster derived from melanopsin‐expressing intrinsic photosensitive retinal ganglion cells. Investigations on the d...
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| Veröffentlicht in: | Synapse (New York, N.Y.) N.Y.), 2023-01, Vol.77 (1), p.e22250-n/a |
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| creator | Reyes‐Méndez, Miriam E. Herrera‐Zamora, J. Manuel Osuna‐Lopez, Fernando Aguilar‐Martínez, Irving S. Góngora‐Alfaro, José L. Navarro‐Polanco, Ricardo A. Sánchez‐Pastor, Enrique Moreno‐Galindo, Eloy G. Alamilla, Javier |
| description | The suprachiasmatic nucleus (SCN) is the most important circadian clock in mammals. The SCN synchronizes to environmental light via the retinohypothalamic tract (RHT), which is an axon cluster derived from melanopsin‐expressing intrinsic photosensitive retinal ganglion cells. Investigations on the development of the nonimage‐forming pathway and the RHT are scarce. Previous studies imply that light stimulation during postnatal development is not needed to make the RHT functional at adult stages. Here, we examined the effects of light deprivation (i.e., constant darkness (DD) rearing) during postnatal development on the expression in the ventral SCN of two crucial proteins for the synchronization of circadian rhythms to light: the presynaptic vesicular glutamate transporter type 2 (vGluT2) and the GluN2B subunit of the postsynaptic NMDA receptor. We found that animals submitted to DD conditions exhibited a transitory reduction in the expression of vGluT2 (at P12–19) and of GluN2B (at P7–9) that was compensated at older stages. These findings support the hypothesis that visual stimulation during early ages is not decisive for normal development of the RHT‐SCN pathway.
Graphical
The SCN synchronizes to environmental light via the RHT. We analyzed the vGluT2 (RHT) and GluN2B (SCN) immunohistochemical expression during the development of rats reared under LD and DD conditions. DD‐exposed animals exhibited a transitory reduction in the VGluT2 and GluN2B expression that was compensated at older stages. These findings support the hypothesis that visual stimulation during early ages is not decisive for normal development of the RHT‐SCN pathway. |
| doi_str_mv | 10.1002/syn.22250 |
| format | Article |
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Graphical
The SCN synchronizes to environmental light via the RHT. We analyzed the vGluT2 (RHT) and GluN2B (SCN) immunohistochemical expression during the development of rats reared under LD and DD conditions. DD‐exposed animals exhibited a transitory reduction in the VGluT2 and GluN2B expression that was compensated at older stages. These findings support the hypothesis that visual stimulation during early ages is not decisive for normal development of the RHT‐SCN pathway.</description><identifier>ISSN: 0887-4476</identifier><identifier>EISSN: 1098-2396</identifier><identifier>DOI: 10.1002/syn.22250</identifier><identifier>PMID: 36085433</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animals ; circadian clock ; Circadian rhythm ; Circadian Rhythm - physiology ; Circadian rhythms ; constant darkness ; development ; glutamate ; Glutamic acid receptors (ionotropic) ; Glutamic acid transporter ; Light effects ; Mammals - metabolism ; Melanopsin ; N-Methyl-D-aspartic acid receptors ; nonimage‐forming system ; Rats ; Receptors, N-Methyl-D-Aspartate - genetics ; Receptors, N-Methyl-D-Aspartate - metabolism ; Retinal ganglion cells ; Retinal Ganglion Cells - metabolism ; Suprachiasmatic nucleus ; Suprachiasmatic Nucleus - metabolism ; Synchronization ; Vesicular Glutamate Transport Protein 2 - metabolism ; Visual stimuli</subject><ispartof>Synapse (New York, N.Y.), 2023-01, Vol.77 (1), p.e22250-n/a</ispartof><rights>2022 Wiley Periodicals LLC.</rights><rights>2023 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2830-768c7435a79801a919902a13aa723966d024f1e34c4d5967e0a1e5a35ea16b6f3</citedby><cites>FETCH-LOGICAL-c2830-768c7435a79801a919902a13aa723966d024f1e34c4d5967e0a1e5a35ea16b6f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fsyn.22250$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fsyn.22250$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36085433$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reyes‐Méndez, Miriam E.</creatorcontrib><creatorcontrib>Herrera‐Zamora, J. Manuel</creatorcontrib><creatorcontrib>Osuna‐Lopez, Fernando</creatorcontrib><creatorcontrib>Aguilar‐Martínez, Irving S.</creatorcontrib><creatorcontrib>Góngora‐Alfaro, José L.</creatorcontrib><creatorcontrib>Navarro‐Polanco, Ricardo A.</creatorcontrib><creatorcontrib>Sánchez‐Pastor, Enrique</creatorcontrib><creatorcontrib>Moreno‐Galindo, Eloy G.</creatorcontrib><creatorcontrib>Alamilla, Javier</creatorcontrib><title>Developmental light deprivation transiently reduces the expression of vGluT2 and GluN2B in the rat ventral suprachiasmatic nucleus</title><title>Synapse (New York, N.Y.)</title><addtitle>Synapse</addtitle><description>The suprachiasmatic nucleus (SCN) is the most important circadian clock in mammals. The SCN synchronizes to environmental light via the retinohypothalamic tract (RHT), which is an axon cluster derived from melanopsin‐expressing intrinsic photosensitive retinal ganglion cells. Investigations on the development of the nonimage‐forming pathway and the RHT are scarce. Previous studies imply that light stimulation during postnatal development is not needed to make the RHT functional at adult stages. Here, we examined the effects of light deprivation (i.e., constant darkness (DD) rearing) during postnatal development on the expression in the ventral SCN of two crucial proteins for the synchronization of circadian rhythms to light: the presynaptic vesicular glutamate transporter type 2 (vGluT2) and the GluN2B subunit of the postsynaptic NMDA receptor. We found that animals submitted to DD conditions exhibited a transitory reduction in the expression of vGluT2 (at P12–19) and of GluN2B (at P7–9) that was compensated at older stages. These findings support the hypothesis that visual stimulation during early ages is not decisive for normal development of the RHT‐SCN pathway.
Graphical
The SCN synchronizes to environmental light via the RHT. We analyzed the vGluT2 (RHT) and GluN2B (SCN) immunohistochemical expression during the development of rats reared under LD and DD conditions. DD‐exposed animals exhibited a transitory reduction in the VGluT2 and GluN2B expression that was compensated at older stages. These findings support the hypothesis that visual stimulation during early ages is not decisive for normal development of the RHT‐SCN pathway.</description><subject>Animals</subject><subject>circadian clock</subject><subject>Circadian rhythm</subject><subject>Circadian Rhythm - physiology</subject><subject>Circadian rhythms</subject><subject>constant darkness</subject><subject>development</subject><subject>glutamate</subject><subject>Glutamic acid receptors (ionotropic)</subject><subject>Glutamic acid transporter</subject><subject>Light effects</subject><subject>Mammals - metabolism</subject><subject>Melanopsin</subject><subject>N-Methyl-D-aspartic acid receptors</subject><subject>nonimage‐forming system</subject><subject>Rats</subject><subject>Receptors, N-Methyl-D-Aspartate - genetics</subject><subject>Receptors, N-Methyl-D-Aspartate - metabolism</subject><subject>Retinal ganglion cells</subject><subject>Retinal Ganglion Cells - metabolism</subject><subject>Suprachiasmatic nucleus</subject><subject>Suprachiasmatic Nucleus - metabolism</subject><subject>Synchronization</subject><subject>Vesicular Glutamate Transport Protein 2 - metabolism</subject><subject>Visual stimuli</subject><issn>0887-4476</issn><issn>1098-2396</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10c1u1DAUBWALUdGhsOAFkCU2sEh7_ZPEWdICpVJVFpQFq-jWuWFSOU6w44HZ8uR4OoVFpa5syZ-PfH0YeyXgWADIk7j1x1LKEp6wlYDGFFI11VO2AmPqQuu6OmTPY7wFACVAP2OHqgJTaqVW7M8H2pCb5pH8go674cd64R3NYdjgMkyeLwF9HPKp2_JAXbIU-bImTr_nQDHuyNTzzblL15Kj73jeXclTPvg7FnDhm3w75PCY5oB2PWAcc7blPllHKb5gBz26SC_v1yP27dPH67PPxeWX84uz95eFlUZBUVfG1lqVWDcGBDaiaUCiUIj1btyqA6l7QUpb3ZVNVROgoBJVSSiqm6pXR-ztPncO089EcWnHIVpyDj1NKbayFtLopoQy0zcP6O2Ugs-vy0oZMALETr3bKxumGAP1bf62EcO2FdDuimlzMe1dMdm-vk9MNyN1_-W_JjI42YNfg6Pt40nt1-9X-8i_iYuYEQ</recordid><startdate>202301</startdate><enddate>202301</enddate><creator>Reyes‐Méndez, Miriam E.</creator><creator>Herrera‐Zamora, J. Manuel</creator><creator>Osuna‐Lopez, Fernando</creator><creator>Aguilar‐Martínez, Irving S.</creator><creator>Góngora‐Alfaro, José L.</creator><creator>Navarro‐Polanco, Ricardo A.</creator><creator>Sánchez‐Pastor, Enrique</creator><creator>Moreno‐Galindo, Eloy G.</creator><creator>Alamilla, Javier</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>202301</creationdate><title>Developmental light deprivation transiently reduces the expression of vGluT2 and GluN2B in the rat ventral suprachiasmatic nucleus</title><author>Reyes‐Méndez, Miriam E. ; Herrera‐Zamora, J. Manuel ; Osuna‐Lopez, Fernando ; Aguilar‐Martínez, Irving S. ; Góngora‐Alfaro, José L. ; Navarro‐Polanco, Ricardo A. ; Sánchez‐Pastor, Enrique ; Moreno‐Galindo, Eloy G. ; Alamilla, Javier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2830-768c7435a79801a919902a13aa723966d024f1e34c4d5967e0a1e5a35ea16b6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>circadian clock</topic><topic>Circadian rhythm</topic><topic>Circadian Rhythm - physiology</topic><topic>Circadian rhythms</topic><topic>constant darkness</topic><topic>development</topic><topic>glutamate</topic><topic>Glutamic acid receptors (ionotropic)</topic><topic>Glutamic acid transporter</topic><topic>Light effects</topic><topic>Mammals - metabolism</topic><topic>Melanopsin</topic><topic>N-Methyl-D-aspartic acid receptors</topic><topic>nonimage‐forming system</topic><topic>Rats</topic><topic>Receptors, N-Methyl-D-Aspartate - genetics</topic><topic>Receptors, N-Methyl-D-Aspartate - metabolism</topic><topic>Retinal ganglion cells</topic><topic>Retinal Ganglion Cells - metabolism</topic><topic>Suprachiasmatic nucleus</topic><topic>Suprachiasmatic Nucleus - metabolism</topic><topic>Synchronization</topic><topic>Vesicular Glutamate Transport Protein 2 - metabolism</topic><topic>Visual stimuli</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reyes‐Méndez, Miriam E.</creatorcontrib><creatorcontrib>Herrera‐Zamora, J. Manuel</creatorcontrib><creatorcontrib>Osuna‐Lopez, Fernando</creatorcontrib><creatorcontrib>Aguilar‐Martínez, Irving S.</creatorcontrib><creatorcontrib>Góngora‐Alfaro, José L.</creatorcontrib><creatorcontrib>Navarro‐Polanco, Ricardo A.</creatorcontrib><creatorcontrib>Sánchez‐Pastor, Enrique</creatorcontrib><creatorcontrib>Moreno‐Galindo, Eloy G.</creatorcontrib><creatorcontrib>Alamilla, Javier</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Synapse (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reyes‐Méndez, Miriam E.</au><au>Herrera‐Zamora, J. Manuel</au><au>Osuna‐Lopez, Fernando</au><au>Aguilar‐Martínez, Irving S.</au><au>Góngora‐Alfaro, José L.</au><au>Navarro‐Polanco, Ricardo A.</au><au>Sánchez‐Pastor, Enrique</au><au>Moreno‐Galindo, Eloy G.</au><au>Alamilla, Javier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental light deprivation transiently reduces the expression of vGluT2 and GluN2B in the rat ventral suprachiasmatic nucleus</atitle><jtitle>Synapse (New York, N.Y.)</jtitle><addtitle>Synapse</addtitle><date>2023-01</date><risdate>2023</risdate><volume>77</volume><issue>1</issue><spage>e22250</spage><epage>n/a</epage><pages>e22250-n/a</pages><issn>0887-4476</issn><eissn>1098-2396</eissn><abstract>The suprachiasmatic nucleus (SCN) is the most important circadian clock in mammals. The SCN synchronizes to environmental light via the retinohypothalamic tract (RHT), which is an axon cluster derived from melanopsin‐expressing intrinsic photosensitive retinal ganglion cells. Investigations on the development of the nonimage‐forming pathway and the RHT are scarce. Previous studies imply that light stimulation during postnatal development is not needed to make the RHT functional at adult stages. Here, we examined the effects of light deprivation (i.e., constant darkness (DD) rearing) during postnatal development on the expression in the ventral SCN of two crucial proteins for the synchronization of circadian rhythms to light: the presynaptic vesicular glutamate transporter type 2 (vGluT2) and the GluN2B subunit of the postsynaptic NMDA receptor. We found that animals submitted to DD conditions exhibited a transitory reduction in the expression of vGluT2 (at P12–19) and of GluN2B (at P7–9) that was compensated at older stages. These findings support the hypothesis that visual stimulation during early ages is not decisive for normal development of the RHT‐SCN pathway.
Graphical
The SCN synchronizes to environmental light via the RHT. We analyzed the vGluT2 (RHT) and GluN2B (SCN) immunohistochemical expression during the development of rats reared under LD and DD conditions. DD‐exposed animals exhibited a transitory reduction in the VGluT2 and GluN2B expression that was compensated at older stages. These findings support the hypothesis that visual stimulation during early ages is not decisive for normal development of the RHT‐SCN pathway.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36085433</pmid><doi>10.1002/syn.22250</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals circadian clock Circadian rhythm Circadian Rhythm - physiology Circadian rhythms constant darkness development glutamate Glutamic acid receptors (ionotropic) Glutamic acid transporter Light effects Mammals - metabolism Melanopsin N-Methyl-D-aspartic acid receptors nonimage‐forming system Rats Receptors, N-Methyl-D-Aspartate - genetics Receptors, N-Methyl-D-Aspartate - metabolism Retinal ganglion cells Retinal Ganglion Cells - metabolism Suprachiasmatic nucleus Suprachiasmatic Nucleus - metabolism Synchronization Vesicular Glutamate Transport Protein 2 - metabolism Visual stimuli |
| title | Developmental light deprivation transiently reduces the expression of vGluT2 and GluN2B in the rat ventral suprachiasmatic nucleus |
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