FUBP1 promotes the proliferation of lung squamous carcinoma cells and regulates tumor immunity through PD-L1
Background and aim: Lung cancer is a common malignancy. Non-small cell lung cancer (NSCLC) is divided into lung squamous cancer (LUSC), large cell carcinoma, and adenocarcinoma. More than 85% of lung cancer cases are NSCLC patients. Further exploration of the pathogenesis of lung cancer is of great...
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Veröffentlicht in: | Allergologia et immunopathologia 2022-01, Vol.50 (5), p.68-74 |
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description | Background and aim: Lung cancer is a common malignancy. Non-small cell lung cancer (NSCLC) is divided into lung squamous cancer (LUSC), large cell carcinoma, and adenocarcinoma. More than 85% of lung cancer cases are NSCLC patients. Further exploration of the pathogenesis of lung cancer is of great significance. In this study, functions of far upstream element-binding protein 1 (FUBP1) on the proliferation and tumor immunity of LUSC cells were evaluated.
Materials and methods: The Cancer Genome Atlas (TCGA) database, Western blot analysis, and immunohistochemistry (IHC) analysis were used to examine the overexpression levels of FUBP1 in LUSC and paracancerous tissues, LUSC cell line, and human normal lung cell line. Then, Western blot assay was employed to validate the transfection efficiency of FUBP1 knockdown in SK-MES-1 cells. Cell counting kit-8 and colony formation assays were used to detect the viability and proliferation of SK-MES-1 cells. Transwell assay was used to examine the migrative and invasive abilities of SK-MES-1 cells. Finally, the xenograft tumor mice model was applied to explore the role of FUBP1 in vivo. IHC assay was used to determine the expression levels FUBP1, PD-L1, and Ki-67. Flow cytometry technology was employed to detect the proportion of CD4+ and CD8+ cells in short sequence negative control (sh-NC) and sh-FUBP1 groups.
Results: Collectively, the results first indicated that FUBP1 was up-regulated in LUSC tissues and cells. It was also demonstrated that knockdown of FUBP1 suppressed cell migration, invasion, and proliferation in lung squamous carcinoma cells. Finally, knockdown of FUBP1 regulated tumor immunity in vivo.
Conclusion: This research suggested that FUBP1 promotes the proliferation of LUSC cells and regulates tumor immunity through programmed death-ligand 1 (PD-L1). |
doi_str_mv | 10.15586/aei.v50i5.659 |
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Materials and methods: The Cancer Genome Atlas (TCGA) database, Western blot analysis, and immunohistochemistry (IHC) analysis were used to examine the overexpression levels of FUBP1 in LUSC and paracancerous tissues, LUSC cell line, and human normal lung cell line. Then, Western blot assay was employed to validate the transfection efficiency of FUBP1 knockdown in SK-MES-1 cells. Cell counting kit-8 and colony formation assays were used to detect the viability and proliferation of SK-MES-1 cells. Transwell assay was used to examine the migrative and invasive abilities of SK-MES-1 cells. Finally, the xenograft tumor mice model was applied to explore the role of FUBP1 in vivo. IHC assay was used to determine the expression levels FUBP1, PD-L1, and Ki-67. Flow cytometry technology was employed to detect the proportion of CD4+ and CD8+ cells in short sequence negative control (sh-NC) and sh-FUBP1 groups.
Results: Collectively, the results first indicated that FUBP1 was up-regulated in LUSC tissues and cells. It was also demonstrated that knockdown of FUBP1 suppressed cell migration, invasion, and proliferation in lung squamous carcinoma cells. Finally, knockdown of FUBP1 regulated tumor immunity in vivo.
Conclusion: This research suggested that FUBP1 promotes the proliferation of LUSC cells and regulates tumor immunity through programmed death-ligand 1 (PD-L1).</description><identifier>ISSN: 0301-0546</identifier><identifier>EISSN: 0301-0546</identifier><identifier>EISSN: 1578-1267</identifier><identifier>DOI: 10.15586/aei.v50i5.659</identifier><language>eng</language><publisher>Murcia: Codon Publications</publisher><subject>Antigens ; Apoptosis ; Cell cycle ; Cell growth ; Fatalities ; Flow cytometry ; Gene expression ; Laboratory animals ; Lung cancer ; Polyclonal antibodies ; Protein expression ; Proteins ; Tumors</subject><ispartof>Allergologia et immunopathologia, 2022-01, Vol.50 (5), p.68-74</ispartof><rights>2022. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c230t-efef917f552ad13d7ee0ec7cd9a32c00e58dd661c7e8b31ac3f8dfb2ad5f222d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Yu, Jie</creatorcontrib><creatorcontrib>Peng, Wen</creatorcontrib><creatorcontrib>Xue, Yingbo</creatorcontrib><creatorcontrib>Li, Yun</creatorcontrib><creatorcontrib>Yang, Lei</creatorcontrib><creatorcontrib>Geng, Yang</creatorcontrib><title>FUBP1 promotes the proliferation of lung squamous carcinoma cells and regulates tumor immunity through PD-L1</title><title>Allergologia et immunopathologia</title><description>Background and aim: Lung cancer is a common malignancy. Non-small cell lung cancer (NSCLC) is divided into lung squamous cancer (LUSC), large cell carcinoma, and adenocarcinoma. More than 85% of lung cancer cases are NSCLC patients. Further exploration of the pathogenesis of lung cancer is of great significance. In this study, functions of far upstream element-binding protein 1 (FUBP1) on the proliferation and tumor immunity of LUSC cells were evaluated.
Materials and methods: The Cancer Genome Atlas (TCGA) database, Western blot analysis, and immunohistochemistry (IHC) analysis were used to examine the overexpression levels of FUBP1 in LUSC and paracancerous tissues, LUSC cell line, and human normal lung cell line. Then, Western blot assay was employed to validate the transfection efficiency of FUBP1 knockdown in SK-MES-1 cells. Cell counting kit-8 and colony formation assays were used to detect the viability and proliferation of SK-MES-1 cells. Transwell assay was used to examine the migrative and invasive abilities of SK-MES-1 cells. Finally, the xenograft tumor mice model was applied to explore the role of FUBP1 in vivo. IHC assay was used to determine the expression levels FUBP1, PD-L1, and Ki-67. Flow cytometry technology was employed to detect the proportion of CD4+ and CD8+ cells in short sequence negative control (sh-NC) and sh-FUBP1 groups.
Results: Collectively, the results first indicated that FUBP1 was up-regulated in LUSC tissues and cells. It was also demonstrated that knockdown of FUBP1 suppressed cell migration, invasion, and proliferation in lung squamous carcinoma cells. Finally, knockdown of FUBP1 regulated tumor immunity in vivo.
Conclusion: This research suggested that FUBP1 promotes the proliferation of LUSC cells and regulates tumor immunity through programmed death-ligand 1 (PD-L1).</description><subject>Antigens</subject><subject>Apoptosis</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Fatalities</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Laboratory animals</subject><subject>Lung cancer</subject><subject>Polyclonal antibodies</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Tumors</subject><issn>0301-0546</issn><issn>0301-0546</issn><issn>1578-1267</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkD1PwzAQhiMEEqWwMltiYUnwR5ykI18FpEp0oLPl2ufWVRK3dozUf4_bMiCmu5MevXrvybJbggvCeVM9SLDFN8eWFxWfnGUjzDDJMS-r8z_7ZXYVwgZjimnFRlk7XTzNCdp617kBAhrWcDhaa8DLwboeOYPa2K9Q2EXZuRiQkl7Z3nUSKWjbgGSvkYdVbOUxIHbOI9t1sbfDPuV5F1drNH_JZ-Q6uzCyDXDzO8fZYvr69fyezz7fPp4fZ7miDA85GDATUhvOqdSE6RoAg6qVnkhGFcbAG62riqgamiUjUjHTaLNMMDeUUs3G2f0pN32yixAG0dlwKCt7SB8IWhPalOUE04Te_UM3Lvo-tUsUxSVpKoYTVZwo5V0IHozYettJvxcEi6N8keSLo3yR5LMfTS16RQ</recordid><startdate>20220101</startdate><enddate>20220101</enddate><creator>Yu, Jie</creator><creator>Peng, Wen</creator><creator>Xue, Yingbo</creator><creator>Li, Yun</creator><creator>Yang, Lei</creator><creator>Geng, Yang</creator><general>Codon Publications</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>COVID</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20220101</creationdate><title>FUBP1 promotes the proliferation of lung squamous carcinoma cells and regulates tumor immunity through PD-L1</title><author>Yu, Jie ; Peng, Wen ; Xue, Yingbo ; Li, Yun ; Yang, Lei ; Geng, Yang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c230t-efef917f552ad13d7ee0ec7cd9a32c00e58dd661c7e8b31ac3f8dfb2ad5f222d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antigens</topic><topic>Apoptosis</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Fatalities</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Laboratory animals</topic><topic>Lung cancer</topic><topic>Polyclonal antibodies</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Jie</creatorcontrib><creatorcontrib>Peng, Wen</creatorcontrib><creatorcontrib>Xue, Yingbo</creatorcontrib><creatorcontrib>Li, Yun</creatorcontrib><creatorcontrib>Yang, Lei</creatorcontrib><creatorcontrib>Geng, Yang</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Allergologia et immunopathologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Jie</au><au>Peng, Wen</au><au>Xue, Yingbo</au><au>Li, Yun</au><au>Yang, Lei</au><au>Geng, Yang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>FUBP1 promotes the proliferation of lung squamous carcinoma cells and regulates tumor immunity through PD-L1</atitle><jtitle>Allergologia et immunopathologia</jtitle><date>2022-01-01</date><risdate>2022</risdate><volume>50</volume><issue>5</issue><spage>68</spage><epage>74</epage><pages>68-74</pages><issn>0301-0546</issn><eissn>0301-0546</eissn><eissn>1578-1267</eissn><abstract>Background and aim: Lung cancer is a common malignancy. Non-small cell lung cancer (NSCLC) is divided into lung squamous cancer (LUSC), large cell carcinoma, and adenocarcinoma. More than 85% of lung cancer cases are NSCLC patients. Further exploration of the pathogenesis of lung cancer is of great significance. In this study, functions of far upstream element-binding protein 1 (FUBP1) on the proliferation and tumor immunity of LUSC cells were evaluated.
Materials and methods: The Cancer Genome Atlas (TCGA) database, Western blot analysis, and immunohistochemistry (IHC) analysis were used to examine the overexpression levels of FUBP1 in LUSC and paracancerous tissues, LUSC cell line, and human normal lung cell line. Then, Western blot assay was employed to validate the transfection efficiency of FUBP1 knockdown in SK-MES-1 cells. Cell counting kit-8 and colony formation assays were used to detect the viability and proliferation of SK-MES-1 cells. Transwell assay was used to examine the migrative and invasive abilities of SK-MES-1 cells. Finally, the xenograft tumor mice model was applied to explore the role of FUBP1 in vivo. IHC assay was used to determine the expression levels FUBP1, PD-L1, and Ki-67. Flow cytometry technology was employed to detect the proportion of CD4+ and CD8+ cells in short sequence negative control (sh-NC) and sh-FUBP1 groups.
Results: Collectively, the results first indicated that FUBP1 was up-regulated in LUSC tissues and cells. It was also demonstrated that knockdown of FUBP1 suppressed cell migration, invasion, and proliferation in lung squamous carcinoma cells. Finally, knockdown of FUBP1 regulated tumor immunity in vivo.
Conclusion: This research suggested that FUBP1 promotes the proliferation of LUSC cells and regulates tumor immunity through programmed death-ligand 1 (PD-L1).</abstract><cop>Murcia</cop><pub>Codon Publications</pub><doi>10.15586/aei.v50i5.659</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Antigens Apoptosis Cell cycle Cell growth Fatalities Flow cytometry Gene expression Laboratory animals Lung cancer Polyclonal antibodies Protein expression Proteins Tumors |
title | FUBP1 promotes the proliferation of lung squamous carcinoma cells and regulates tumor immunity through PD-L1 |
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