CD24 blockade promotes anti‐tumor immunity in oral squamous cell carcinoma
Objectives Our study elucidates the prognostic role of cluster of differentiation (CD) 24 expression in oral squamous cell carcinoma (OSCC) and determines whether targeting CD24 enhances the anti‐tumor immune response by inhibiting tumor‐associated macrophages (TAMs). Materials and Methods The expre...
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| Veröffentlicht in: | Oral diseases 2024-03, Vol.30 (2), p.163-171 |
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| container_title | Oral diseases |
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| creator | Zou, Ke‐Long Lan, Zhou Cui, Hao Zhao, Yu‐Yue Wang, Wei‐Ming Yu, Guang‐Tao |
| description | Objectives
Our study elucidates the prognostic role of cluster of differentiation (CD) 24 expression in oral squamous cell carcinoma (OSCC) and determines whether targeting CD24 enhances the anti‐tumor immune response by inhibiting tumor‐associated macrophages (TAMs).
Materials and Methods
The expression of CD24 and CD68 was analyzed immunohistochemically via tissue microarrays constructed using 56 cohorts of patients with OSCC and 20 control specimens. Further, CD24 was inhibited in an allograft squamous cell carcinoma (SCC) related mouse model with CD24mAb to determine the tumor volume and weight. Changes in immune cells such as TAMs and T cells in the tumor microenvironment (TME) were analyzed by Flow cytometry. The expression of CD4, CD8, and Ki67 was analyzed via immunohistochemistry. The inhibition of CD24 was confirmed by Western blot and immunohistochemistry.
Results
CD24 was overexpressed in OSCC. High expression of CD24 indicated poor survival in patients with OSCC (p = 0.0334). CD24 expression was significantly correlated with CD68 (p = 0.0424). The inhibition of CD24 delayed tumor growth in vivo. A decrease in TAMs number and an increase in T cell number were confirmed, while the ability of tumor proliferation was impaired.
Conclusion
Targeting CD24 could enhance anti‐tumor immune response by inhibiting TAMs. |
| doi_str_mv | 10.1111/odi.14367 |
| format | Article |
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Our study elucidates the prognostic role of cluster of differentiation (CD) 24 expression in oral squamous cell carcinoma (OSCC) and determines whether targeting CD24 enhances the anti‐tumor immune response by inhibiting tumor‐associated macrophages (TAMs).
Materials and Methods
The expression of CD24 and CD68 was analyzed immunohistochemically via tissue microarrays constructed using 56 cohorts of patients with OSCC and 20 control specimens. Further, CD24 was inhibited in an allograft squamous cell carcinoma (SCC) related mouse model with CD24mAb to determine the tumor volume and weight. Changes in immune cells such as TAMs and T cells in the tumor microenvironment (TME) were analyzed by Flow cytometry. The expression of CD4, CD8, and Ki67 was analyzed via immunohistochemistry. The inhibition of CD24 was confirmed by Western blot and immunohistochemistry.
Results
CD24 was overexpressed in OSCC. High expression of CD24 indicated poor survival in patients with OSCC (p = 0.0334). CD24 expression was significantly correlated with CD68 (p = 0.0424). The inhibition of CD24 delayed tumor growth in vivo. A decrease in TAMs number and an increase in T cell number were confirmed, while the ability of tumor proliferation was impaired.
Conclusion
Targeting CD24 could enhance anti‐tumor immune response by inhibiting TAMs.</description><identifier>ISSN: 1354-523X</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.14367</identifier><identifier>PMID: 36056698</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Cancer ; CD24 ; CD4 antigen ; CD8 antigen ; Cell number ; Flow cytometry ; Gene expression ; Immunohistochemistry ; immunotherapy ; Lymphocytes T ; Macrophages ; Medical prognosis ; oncology ; Oral cancer ; Oral carcinoma ; Oral squamous cell carcinoma ; Squamous cell carcinoma ; Tumor microenvironment ; Tumors ; tumor‐associated macrophages</subject><ispartof>Oral diseases, 2024-03, Vol.30 (2), p.163-171</ispartof><rights>2022 Wiley Periodicals LLC.</rights><rights>2024 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3537-c51ecb8c7d3b1ef2c40a4ef2fb5063b13fd5c39f62d70c599cad3ee16890c04f3</citedby><cites>FETCH-LOGICAL-c3537-c51ecb8c7d3b1ef2c40a4ef2fb5063b13fd5c39f62d70c599cad3ee16890c04f3</cites><orcidid>0000-0003-1642-3924</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.14367$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.14367$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36056698$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zou, Ke‐Long</creatorcontrib><creatorcontrib>Lan, Zhou</creatorcontrib><creatorcontrib>Cui, Hao</creatorcontrib><creatorcontrib>Zhao, Yu‐Yue</creatorcontrib><creatorcontrib>Wang, Wei‐Ming</creatorcontrib><creatorcontrib>Yu, Guang‐Tao</creatorcontrib><title>CD24 blockade promotes anti‐tumor immunity in oral squamous cell carcinoma</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objectives
Our study elucidates the prognostic role of cluster of differentiation (CD) 24 expression in oral squamous cell carcinoma (OSCC) and determines whether targeting CD24 enhances the anti‐tumor immune response by inhibiting tumor‐associated macrophages (TAMs).
Materials and Methods
The expression of CD24 and CD68 was analyzed immunohistochemically via tissue microarrays constructed using 56 cohorts of patients with OSCC and 20 control specimens. Further, CD24 was inhibited in an allograft squamous cell carcinoma (SCC) related mouse model with CD24mAb to determine the tumor volume and weight. Changes in immune cells such as TAMs and T cells in the tumor microenvironment (TME) were analyzed by Flow cytometry. The expression of CD4, CD8, and Ki67 was analyzed via immunohistochemistry. The inhibition of CD24 was confirmed by Western blot and immunohistochemistry.
Results
CD24 was overexpressed in OSCC. High expression of CD24 indicated poor survival in patients with OSCC (p = 0.0334). CD24 expression was significantly correlated with CD68 (p = 0.0424). The inhibition of CD24 delayed tumor growth in vivo. A decrease in TAMs number and an increase in T cell number were confirmed, while the ability of tumor proliferation was impaired.
Conclusion
Targeting CD24 could enhance anti‐tumor immune response by inhibiting TAMs.</description><subject>Cancer</subject><subject>CD24</subject><subject>CD4 antigen</subject><subject>CD8 antigen</subject><subject>Cell number</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Immunohistochemistry</subject><subject>immunotherapy</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>Medical prognosis</subject><subject>oncology</subject><subject>Oral cancer</subject><subject>Oral carcinoma</subject><subject>Oral squamous cell carcinoma</subject><subject>Squamous cell carcinoma</subject><subject>Tumor microenvironment</subject><subject>Tumors</subject><subject>tumor‐associated macrophages</subject><issn>1354-523X</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp1kM1KAzEURoMoVqsLX0ACbnQxbTKZTCZLaf0pFLpRcBcymQykTiZtMoN05yP4jD6Jqa0uBO_i3hAOHx8HgAuMRjjO2FVmhDOSswNwgnOEE1Sk9DC-Cc0SmpKXATgNYYkQZpykx2BAckTznBcnYD6ZphksG6deZaXhyjvrOh2gbDvz-f7R9dZ5aKztW9NtoGmh87KBYd1L6_oAlW4aqKRXpnVWnoGjWjZBn-_vEDzf3z1NHpP54mE2uZ0nilDCEkWxVmWhWEVKrOtUZUhm8dYlRXn8InVFFeF1nlYMKcq5khXRGucFRwplNRmC611urLvudeiENWFbRbY6thIpQ5wRxuMagqs_6NL1vo3tRBpdcMZYQSJ1s6OUdyF4XYuVN1b6jcBIbBWLqFh8K47s5T6xL62ufskfpxEY74A30-jN_0liMZ3tIr8AU7GGHw</recordid><startdate>202403</startdate><enddate>202403</enddate><creator>Zou, Ke‐Long</creator><creator>Lan, Zhou</creator><creator>Cui, Hao</creator><creator>Zhao, Yu‐Yue</creator><creator>Wang, Wei‐Ming</creator><creator>Yu, Guang‐Tao</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1642-3924</orcidid></search><sort><creationdate>202403</creationdate><title>CD24 blockade promotes anti‐tumor immunity in oral squamous cell carcinoma</title><author>Zou, Ke‐Long ; Lan, Zhou ; Cui, Hao ; Zhao, Yu‐Yue ; Wang, Wei‐Ming ; Yu, Guang‐Tao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3537-c51ecb8c7d3b1ef2c40a4ef2fb5063b13fd5c39f62d70c599cad3ee16890c04f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Cancer</topic><topic>CD24</topic><topic>CD4 antigen</topic><topic>CD8 antigen</topic><topic>Cell number</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Immunohistochemistry</topic><topic>immunotherapy</topic><topic>Lymphocytes T</topic><topic>Macrophages</topic><topic>Medical prognosis</topic><topic>oncology</topic><topic>Oral cancer</topic><topic>Oral carcinoma</topic><topic>Oral squamous cell carcinoma</topic><topic>Squamous cell carcinoma</topic><topic>Tumor microenvironment</topic><topic>Tumors</topic><topic>tumor‐associated macrophages</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zou, Ke‐Long</creatorcontrib><creatorcontrib>Lan, Zhou</creatorcontrib><creatorcontrib>Cui, Hao</creatorcontrib><creatorcontrib>Zhao, Yu‐Yue</creatorcontrib><creatorcontrib>Wang, Wei‐Ming</creatorcontrib><creatorcontrib>Yu, Guang‐Tao</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zou, Ke‐Long</au><au>Lan, Zhou</au><au>Cui, Hao</au><au>Zhao, Yu‐Yue</au><au>Wang, Wei‐Ming</au><au>Yu, Guang‐Tao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CD24 blockade promotes anti‐tumor immunity in oral squamous cell carcinoma</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2024-03</date><risdate>2024</risdate><volume>30</volume><issue>2</issue><spage>163</spage><epage>171</epage><pages>163-171</pages><issn>1354-523X</issn><eissn>1601-0825</eissn><abstract>Objectives
Our study elucidates the prognostic role of cluster of differentiation (CD) 24 expression in oral squamous cell carcinoma (OSCC) and determines whether targeting CD24 enhances the anti‐tumor immune response by inhibiting tumor‐associated macrophages (TAMs).
Materials and Methods
The expression of CD24 and CD68 was analyzed immunohistochemically via tissue microarrays constructed using 56 cohorts of patients with OSCC and 20 control specimens. Further, CD24 was inhibited in an allograft squamous cell carcinoma (SCC) related mouse model with CD24mAb to determine the tumor volume and weight. Changes in immune cells such as TAMs and T cells in the tumor microenvironment (TME) were analyzed by Flow cytometry. The expression of CD4, CD8, and Ki67 was analyzed via immunohistochemistry. The inhibition of CD24 was confirmed by Western blot and immunohistochemistry.
Results
CD24 was overexpressed in OSCC. High expression of CD24 indicated poor survival in patients with OSCC (p = 0.0334). CD24 expression was significantly correlated with CD68 (p = 0.0424). The inhibition of CD24 delayed tumor growth in vivo. A decrease in TAMs number and an increase in T cell number were confirmed, while the ability of tumor proliferation was impaired.
Conclusion
Targeting CD24 could enhance anti‐tumor immune response by inhibiting TAMs.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36056698</pmid><doi>10.1111/odi.14367</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-1642-3924</orcidid></addata></record> |
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| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Cancer CD24 CD4 antigen CD8 antigen Cell number Flow cytometry Gene expression Immunohistochemistry immunotherapy Lymphocytes T Macrophages Medical prognosis oncology Oral cancer Oral carcinoma Oral squamous cell carcinoma Squamous cell carcinoma Tumor microenvironment Tumors tumor‐associated macrophages |
| title | CD24 blockade promotes anti‐tumor immunity in oral squamous cell carcinoma |
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