Poly(caprolactone)‐aligned nanofibers associated with fibronectin‐loaded collagen hydrogel as a potent bioactive scaffold for cell‐free regenerative endodontics
Aim Guided tissue regeneration has been considered a promising strategy to replace conventional endodontic therapy of teeth with incomplete root formation. Therefore, the objective of this study was to develop a tubular scaffold (TB‐SC) with poly (caprolactone)‐aligned nanofibres associated with a f...
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| Veröffentlicht in: | International endodontic journal 2022-12, Vol.55 (12), p.1359-1371 |
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| creator | Leite, Maria Luísa Oliveira Ribeiro, Rafael Antônio Soares, Diana Gabriela Hebling, Josimeri Souza Costa, Carlos Alberto |
| description | Aim
Guided tissue regeneration has been considered a promising strategy to replace conventional endodontic therapy of teeth with incomplete root formation. Therefore, the objective of this study was to develop a tubular scaffold (TB‐SC) with poly (caprolactone)‐aligned nanofibres associated with a fibronectin (FN)‐loaded collagen hydrogel and assess the pulp regeneration potential mediated by human apical papilla cells (hAPCs) using an in vitro model of teeth with incomplete root formation.
Methodology
Aligned nanofibre strips based on 10% poly(caprolactone) (PCL) were synthesized with the electrospinning technique to produce the TB‐SCs. These were submitted to different treatments, according to the following groups: TB‐SC (negative control): TB‐SC without treatment; TB‐SC + FN (positive control): TB‐SC coated with 10 μg/ml of FN; TB‐SC + H: TB‐SC associated with collagen hydrogel; TB‐SC + HFN: TB‐SC associated with FN‐loaded collagen hydrogel. Then, the biomaterials were inserted into cylindrical devices to mimic the regenerative therapy of teeth with incomplete root formation. The hAPCs were seeded on the upper surface of the TB‐SCs associated or not with any treatment, and cell migration/proliferation and the gene expression of markers related to pulp regeneration (ITGA5, ITGAV, COL1A1 and COL1A3) were evaluated. The data were submitted to anova/Tukey's tests (α = 5%).
Results
Higher values of cell migration/proliferation and gene expression of all markers tested were observed in groups TB‐SC + FN, TB‐SC + H, and TB‐SC + HFN compared with the TB‐SC group (p |
| doi_str_mv | 10.1111/iej.13823 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2707874960</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2707874960</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3303-7272ad0924771f6a5654723acad0b9a9c417d008274f9c80f11bd218f871b0c03</originalsourceid><addsrcrecordid>eNp1kU1uFDEQhS1EJIaEBTewxCZZdOKf7nH3EkUBgiLBIlm3qu3yxCPHHuweotlxBE6Rg-Uk1GRYIVGbkp6_V1XyY-y9FOeS6iLg-lzqXulXbCH1smtUN8jXbCFkqxvV990b9rbWtRCiE1ou2NP3HHenFjYlR7BzTnj2_Os3xLBK6HiClH2YsFQOtWYbYCb1Mcz3nORCtJ1DIkPM4OjF5hhhhYnf71zJK4xk48A3ecY08ylkWhF-Iq8WvM_RcZ8LtxgjjfAFkRckNxZ4oTC57HKag60n7MhDrPjubz9md5-ubi-_NDffPl9ffrxprNZCN0YZBU4MqjVG-iV0y641SoMlcRpgsK00TohemdYPthdeyskp2fveyElYoY_Z6WEu_cePLdZ5fAh1fyAkzNs6KiNMb9phuUc__IOu87Ykuo4o3bZSGD0QdXagbMm1FvTjpoQHKLtRinGf2EiJjS-JEXtxYB9DxN3_wfH66uvB8QeLGp1v</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2734410739</pqid></control><display><type>article</type><title>Poly(caprolactone)‐aligned nanofibers associated with fibronectin‐loaded collagen hydrogel as a potent bioactive scaffold for cell‐free regenerative endodontics</title><source>Wiley Journals</source><creator>Leite, Maria Luísa ; Oliveira Ribeiro, Rafael Antônio ; Soares, Diana Gabriela ; Hebling, Josimeri ; Souza Costa, Carlos Alberto</creator><creatorcontrib>Leite, Maria Luísa ; Oliveira Ribeiro, Rafael Antônio ; Soares, Diana Gabriela ; Hebling, Josimeri ; Souza Costa, Carlos Alberto</creatorcontrib><description>Aim
Guided tissue regeneration has been considered a promising strategy to replace conventional endodontic therapy of teeth with incomplete root formation. Therefore, the objective of this study was to develop a tubular scaffold (TB‐SC) with poly (caprolactone)‐aligned nanofibres associated with a fibronectin (FN)‐loaded collagen hydrogel and assess the pulp regeneration potential mediated by human apical papilla cells (hAPCs) using an in vitro model of teeth with incomplete root formation.
Methodology
Aligned nanofibre strips based on 10% poly(caprolactone) (PCL) were synthesized with the electrospinning technique to produce the TB‐SCs. These were submitted to different treatments, according to the following groups: TB‐SC (negative control): TB‐SC without treatment; TB‐SC + FN (positive control): TB‐SC coated with 10 μg/ml of FN; TB‐SC + H: TB‐SC associated with collagen hydrogel; TB‐SC + HFN: TB‐SC associated with FN‐loaded collagen hydrogel. Then, the biomaterials were inserted into cylindrical devices to mimic the regenerative therapy of teeth with incomplete root formation. The hAPCs were seeded on the upper surface of the TB‐SCs associated or not with any treatment, and cell migration/proliferation and the gene expression of markers related to pulp regeneration (ITGA5, ITGAV, COL1A1 and COL1A3) were evaluated. The data were submitted to anova/Tukey's tests (α = 5%).
Results
Higher values of cell migration/proliferation and gene expression of all markers tested were observed in groups TB‐SC + FN, TB‐SC + H, and TB‐SC + HFN compared with the TB‐SC group (p < .05). The hAPCs in the TB‐SC + HFN group showed the highest values of cell proliferation and gene expression of COL1A1 and COL3A1 (p < .05), as well as superior cell migration results to groups TB‐SC and TB‐SC + H (p < .05).
Conclusion
Aligned nanofibre scaffolds associated with the FN‐loaded collagen hydrogel enhanced the migration and proliferation of hAPCs and gene expression of pulp regeneration markers. Therefore, the use of these biomaterials may be considered an interesting strategy for regenerative pulp therapy of teeth with incomplete root formation.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.13823</identifier><language>eng</language><publisher>Chichester: Wiley Subscription Services, Inc</publisher><subject>apical papilla cells ; Biomaterials ; Biomedical materials ; Cell adhesion & migration ; Cell migration ; Cell proliferation ; Collagen ; Collagen (type I) ; Dental pulp ; Fibronectin ; Gene expression ; hydrogel ; Hydrogels ; nanofibres ; Polycaprolactone ; pulp regeneration ; Teeth ; Tissue engineering</subject><ispartof>International endodontic journal, 2022-12, Vol.55 (12), p.1359-1371</ispartof><rights>2022 British Endodontic Society. Published by John Wiley & Sons Ltd</rights><rights>Copyright © 2022 International Endodontic Journal. Published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3303-7272ad0924771f6a5654723acad0b9a9c417d008274f9c80f11bd218f871b0c03</citedby><cites>FETCH-LOGICAL-c3303-7272ad0924771f6a5654723acad0b9a9c417d008274f9c80f11bd218f871b0c03</cites><orcidid>0000-0002-7455-6867 ; 0000-0001-8100-3294 ; 0000-0001-6650-3637 ; 0000-0002-1485-6104 ; 0000-0002-2846-2325</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fiej.13823$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fiej.13823$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Leite, Maria Luísa</creatorcontrib><creatorcontrib>Oliveira Ribeiro, Rafael Antônio</creatorcontrib><creatorcontrib>Soares, Diana Gabriela</creatorcontrib><creatorcontrib>Hebling, Josimeri</creatorcontrib><creatorcontrib>Souza Costa, Carlos Alberto</creatorcontrib><title>Poly(caprolactone)‐aligned nanofibers associated with fibronectin‐loaded collagen hydrogel as a potent bioactive scaffold for cell‐free regenerative endodontics</title><title>International endodontic journal</title><description>Aim
Guided tissue regeneration has been considered a promising strategy to replace conventional endodontic therapy of teeth with incomplete root formation. Therefore, the objective of this study was to develop a tubular scaffold (TB‐SC) with poly (caprolactone)‐aligned nanofibres associated with a fibronectin (FN)‐loaded collagen hydrogel and assess the pulp regeneration potential mediated by human apical papilla cells (hAPCs) using an in vitro model of teeth with incomplete root formation.
Methodology
Aligned nanofibre strips based on 10% poly(caprolactone) (PCL) were synthesized with the electrospinning technique to produce the TB‐SCs. These were submitted to different treatments, according to the following groups: TB‐SC (negative control): TB‐SC without treatment; TB‐SC + FN (positive control): TB‐SC coated with 10 μg/ml of FN; TB‐SC + H: TB‐SC associated with collagen hydrogel; TB‐SC + HFN: TB‐SC associated with FN‐loaded collagen hydrogel. Then, the biomaterials were inserted into cylindrical devices to mimic the regenerative therapy of teeth with incomplete root formation. The hAPCs were seeded on the upper surface of the TB‐SCs associated or not with any treatment, and cell migration/proliferation and the gene expression of markers related to pulp regeneration (ITGA5, ITGAV, COL1A1 and COL1A3) were evaluated. The data were submitted to anova/Tukey's tests (α = 5%).
Results
Higher values of cell migration/proliferation and gene expression of all markers tested were observed in groups TB‐SC + FN, TB‐SC + H, and TB‐SC + HFN compared with the TB‐SC group (p < .05). The hAPCs in the TB‐SC + HFN group showed the highest values of cell proliferation and gene expression of COL1A1 and COL3A1 (p < .05), as well as superior cell migration results to groups TB‐SC and TB‐SC + H (p < .05).
Conclusion
Aligned nanofibre scaffolds associated with the FN‐loaded collagen hydrogel enhanced the migration and proliferation of hAPCs and gene expression of pulp regeneration markers. Therefore, the use of these biomaterials may be considered an interesting strategy for regenerative pulp therapy of teeth with incomplete root formation.</description><subject>apical papilla cells</subject><subject>Biomaterials</subject><subject>Biomedical materials</subject><subject>Cell adhesion & migration</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Collagen</subject><subject>Collagen (type I)</subject><subject>Dental pulp</subject><subject>Fibronectin</subject><subject>Gene expression</subject><subject>hydrogel</subject><subject>Hydrogels</subject><subject>nanofibres</subject><subject>Polycaprolactone</subject><subject>pulp regeneration</subject><subject>Teeth</subject><subject>Tissue engineering</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kU1uFDEQhS1EJIaEBTewxCZZdOKf7nH3EkUBgiLBIlm3qu3yxCPHHuweotlxBE6Rg-Uk1GRYIVGbkp6_V1XyY-y9FOeS6iLg-lzqXulXbCH1smtUN8jXbCFkqxvV990b9rbWtRCiE1ou2NP3HHenFjYlR7BzTnj2_Os3xLBK6HiClH2YsFQOtWYbYCb1Mcz3nORCtJ1DIkPM4OjF5hhhhYnf71zJK4xk48A3ecY08ylkWhF-Iq8WvM_RcZ8LtxgjjfAFkRckNxZ4oTC57HKag60n7MhDrPjubz9md5-ubi-_NDffPl9ffrxprNZCN0YZBU4MqjVG-iV0y641SoMlcRpgsK00TohemdYPthdeyskp2fveyElYoY_Z6WEu_cePLdZ5fAh1fyAkzNs6KiNMb9phuUc__IOu87Ykuo4o3bZSGD0QdXagbMm1FvTjpoQHKLtRinGf2EiJjS-JEXtxYB9DxN3_wfH66uvB8QeLGp1v</recordid><startdate>202212</startdate><enddate>202212</enddate><creator>Leite, Maria Luísa</creator><creator>Oliveira Ribeiro, Rafael Antônio</creator><creator>Soares, Diana Gabriela</creator><creator>Hebling, Josimeri</creator><creator>Souza Costa, Carlos Alberto</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7455-6867</orcidid><orcidid>https://orcid.org/0000-0001-8100-3294</orcidid><orcidid>https://orcid.org/0000-0001-6650-3637</orcidid><orcidid>https://orcid.org/0000-0002-1485-6104</orcidid><orcidid>https://orcid.org/0000-0002-2846-2325</orcidid></search><sort><creationdate>202212</creationdate><title>Poly(caprolactone)‐aligned nanofibers associated with fibronectin‐loaded collagen hydrogel as a potent bioactive scaffold for cell‐free regenerative endodontics</title><author>Leite, Maria Luísa ; Oliveira Ribeiro, Rafael Antônio ; Soares, Diana Gabriela ; Hebling, Josimeri ; Souza Costa, Carlos Alberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3303-7272ad0924771f6a5654723acad0b9a9c417d008274f9c80f11bd218f871b0c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>apical papilla cells</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>Cell adhesion & migration</topic><topic>Cell migration</topic><topic>Cell proliferation</topic><topic>Collagen</topic><topic>Collagen (type I)</topic><topic>Dental pulp</topic><topic>Fibronectin</topic><topic>Gene expression</topic><topic>hydrogel</topic><topic>Hydrogels</topic><topic>nanofibres</topic><topic>Polycaprolactone</topic><topic>pulp regeneration</topic><topic>Teeth</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leite, Maria Luísa</creatorcontrib><creatorcontrib>Oliveira Ribeiro, Rafael Antônio</creatorcontrib><creatorcontrib>Soares, Diana Gabriela</creatorcontrib><creatorcontrib>Hebling, Josimeri</creatorcontrib><creatorcontrib>Souza Costa, Carlos Alberto</creatorcontrib><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leite, Maria Luísa</au><au>Oliveira Ribeiro, Rafael Antônio</au><au>Soares, Diana Gabriela</au><au>Hebling, Josimeri</au><au>Souza Costa, Carlos Alberto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Poly(caprolactone)‐aligned nanofibers associated with fibronectin‐loaded collagen hydrogel as a potent bioactive scaffold for cell‐free regenerative endodontics</atitle><jtitle>International endodontic journal</jtitle><date>2022-12</date><risdate>2022</risdate><volume>55</volume><issue>12</issue><spage>1359</spage><epage>1371</epage><pages>1359-1371</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Aim
Guided tissue regeneration has been considered a promising strategy to replace conventional endodontic therapy of teeth with incomplete root formation. Therefore, the objective of this study was to develop a tubular scaffold (TB‐SC) with poly (caprolactone)‐aligned nanofibres associated with a fibronectin (FN)‐loaded collagen hydrogel and assess the pulp regeneration potential mediated by human apical papilla cells (hAPCs) using an in vitro model of teeth with incomplete root formation.
Methodology
Aligned nanofibre strips based on 10% poly(caprolactone) (PCL) were synthesized with the electrospinning technique to produce the TB‐SCs. These were submitted to different treatments, according to the following groups: TB‐SC (negative control): TB‐SC without treatment; TB‐SC + FN (positive control): TB‐SC coated with 10 μg/ml of FN; TB‐SC + H: TB‐SC associated with collagen hydrogel; TB‐SC + HFN: TB‐SC associated with FN‐loaded collagen hydrogel. Then, the biomaterials were inserted into cylindrical devices to mimic the regenerative therapy of teeth with incomplete root formation. The hAPCs were seeded on the upper surface of the TB‐SCs associated or not with any treatment, and cell migration/proliferation and the gene expression of markers related to pulp regeneration (ITGA5, ITGAV, COL1A1 and COL1A3) were evaluated. The data were submitted to anova/Tukey's tests (α = 5%).
Results
Higher values of cell migration/proliferation and gene expression of all markers tested were observed in groups TB‐SC + FN, TB‐SC + H, and TB‐SC + HFN compared with the TB‐SC group (p < .05). The hAPCs in the TB‐SC + HFN group showed the highest values of cell proliferation and gene expression of COL1A1 and COL3A1 (p < .05), as well as superior cell migration results to groups TB‐SC and TB‐SC + H (p < .05).
Conclusion
Aligned nanofibre scaffolds associated with the FN‐loaded collagen hydrogel enhanced the migration and proliferation of hAPCs and gene expression of pulp regeneration markers. Therefore, the use of these biomaterials may be considered an interesting strategy for regenerative pulp therapy of teeth with incomplete root formation.</abstract><cop>Chichester</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/iej.13823</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-7455-6867</orcidid><orcidid>https://orcid.org/0000-0001-8100-3294</orcidid><orcidid>https://orcid.org/0000-0001-6650-3637</orcidid><orcidid>https://orcid.org/0000-0002-1485-6104</orcidid><orcidid>https://orcid.org/0000-0002-2846-2325</orcidid></addata></record> |
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| subjects | apical papilla cells Biomaterials Biomedical materials Cell adhesion & migration Cell migration Cell proliferation Collagen Collagen (type I) Dental pulp Fibronectin Gene expression hydrogel Hydrogels nanofibres Polycaprolactone pulp regeneration Teeth Tissue engineering |
| title | Poly(caprolactone)‐aligned nanofibers associated with fibronectin‐loaded collagen hydrogel as a potent bioactive scaffold for cell‐free regenerative endodontics |
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