Mapping and identification of a new potential dominant resistance gene to turnip mosaic virus in Brassica rapa
Main conclusion By constructing an F 2 population, a new potential dominant resistance gene to TuMV in Brassica rapa was mapped and identified. Brassica rapa is the most widely grown vegetable crop in China, and turnip mosaic virus (TuMV) is a great threat to its production. Hence, it is a very impo...
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| Veröffentlicht in: | Planta 2022-10, Vol.256 (4), p.66-66, Article 66 |
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| creator | Lu, Xinxin Li, Ze Huang, Wenyue Wang, Shaoxing Zhang, Shifan Li, Fei Zhang, Hui Sun, Rifei Li, Guoliang Zhang, Shujiang |
| description | Main conclusion
By constructing an F
2
population, a new potential dominant resistance gene to TuMV in
Brassica rapa
was mapped and identified.
Brassica rapa
is the most widely grown vegetable crop in China, and turnip mosaic virus (TuMV) is a great threat to its production. Hence, it is a very important work to excavate more and novel resistance genes in
B. rapa
. In this study, the resistant line B80124 and the susceptible line B80450 were used to construct the F
2
populations, and through genetic analysis, the resistance to TuMV was found to be controlled by a dominant gene. Bulked segregant analysis sequence (BSA-seq) was used for the primary mapping, and an intersection (22.25–25.03 Mb) was obtained. After fine mapping using single nucleotide polymorphisms (SNP) markers, the candidate region was narrowed to 330 kb between the SNP markers A06S11 and A06S14, including eight genes relating to disease resistance. Using the transcriptome analysis and sequence identification,
BraA06g035130.3C
was screened as the final candidate gene, and it contained two deletion mutations, leading to frameshift in the susceptible line B80450. In addition, the phylogenetic analysis, hydrophilia and hydrophobicity analysis, subcellular location prediction analysis, amino acid bias analysis, and 3D modeling structures of
BraA06g035130.3C
were conducted to predict its functions. This study was conducive to the identification of a new TuMV resistance gene in
B. rapa
, which is of important scientific significance and application value for the improvement of TuMV resistance traits and molecular design breeding for Brassica crops. |
| doi_str_mv | 10.1007/s00425-022-03981-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2707874551</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2707874551</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-71df2471d6096256f68b48d42edcbb8e784fdb3e253d7428d0380b877f2c484e3</originalsourceid><addsrcrecordid>eNp9kUtLAzEUhYMoWKt_wFXAjZvRO3lM0qUWX1Bxo-uQmWRKSpuMSUbx35u2guDCLJIL5zuHGw5C5zVc1QDiOgEwwisgpAI6k3XFD9CkZpRUBJg8RBOAMsOM8mN0ktIKoIhCTJB_1sPg_BJrb7Az1mfXu05nFzwOPdbY2088hLwV9BqbsHFe-4yjTS5l7TuLl9ZbnAPOY_RuwJuQtOvwh4tjws7j26hTKpE46kGfoqNer5M9-3mn6O3-7nX-WC1eHp7mN4uqo5zkStSmJ6zcDcwawpu-kS2ThhFruraVVkjWm5ZawqkRjEgDVEIrhehJxySzdIou97lDDO-jTVltXOrseq29DWNSRICQgnFeF_TiD7oK5Sdlux0lSFNOocie6mJIKdpeDdFtdPxSNahtBWpfgSoVqF0FihcT3ZtSgf3Sxt_of1zf7kKJkg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2707726666</pqid></control><display><type>article</type><title>Mapping and identification of a new potential dominant resistance gene to turnip mosaic virus in Brassica rapa</title><source>Springer Journals</source><creator>Lu, Xinxin ; Li, Ze ; Huang, Wenyue ; Wang, Shaoxing ; Zhang, Shifan ; Li, Fei ; Zhang, Hui ; Sun, Rifei ; Li, Guoliang ; Zhang, Shujiang</creator><creatorcontrib>Lu, Xinxin ; Li, Ze ; Huang, Wenyue ; Wang, Shaoxing ; Zhang, Shifan ; Li, Fei ; Zhang, Hui ; Sun, Rifei ; Li, Guoliang ; Zhang, Shujiang</creatorcontrib><description>Main conclusion
By constructing an F
2
population, a new potential dominant resistance gene to TuMV in
Brassica rapa
was mapped and identified.
Brassica rapa
is the most widely grown vegetable crop in China, and turnip mosaic virus (TuMV) is a great threat to its production. Hence, it is a very important work to excavate more and novel resistance genes in
B. rapa
. In this study, the resistant line B80124 and the susceptible line B80450 were used to construct the F
2
populations, and through genetic analysis, the resistance to TuMV was found to be controlled by a dominant gene. Bulked segregant analysis sequence (BSA-seq) was used for the primary mapping, and an intersection (22.25–25.03 Mb) was obtained. After fine mapping using single nucleotide polymorphisms (SNP) markers, the candidate region was narrowed to 330 kb between the SNP markers A06S11 and A06S14, including eight genes relating to disease resistance. Using the transcriptome analysis and sequence identification,
BraA06g035130.3C
was screened as the final candidate gene, and it contained two deletion mutations, leading to frameshift in the susceptible line B80450. In addition, the phylogenetic analysis, hydrophilia and hydrophobicity analysis, subcellular location prediction analysis, amino acid bias analysis, and 3D modeling structures of
BraA06g035130.3C
were conducted to predict its functions. This study was conducive to the identification of a new TuMV resistance gene in
B. rapa
, which is of important scientific significance and application value for the improvement of TuMV resistance traits and molecular design breeding for Brassica crops.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-022-03981-5</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Agriculture ; Amino acids ; Biomedical and Life Sciences ; Brassica ; Brassica rapa ; Disease resistance ; Ecology ; Forestry ; Frameshift mutation ; Gene deletion ; Gene mapping ; Genes ; Genetic analysis ; Hydrophobicity ; Life Sciences ; Mapping ; Markers ; Mutation ; Nucleotides ; Original Article ; Phylogeny ; Plant breeding ; Plant Sciences ; Population genetics ; Single-nucleotide polymorphism ; Three dimensional models ; Transcriptomes ; Turnips ; Viruses</subject><ispartof>Planta, 2022-10, Vol.256 (4), p.66-66, Article 66</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-71df2471d6096256f68b48d42edcbb8e784fdb3e253d7428d0380b877f2c484e3</citedby><cites>FETCH-LOGICAL-c352t-71df2471d6096256f68b48d42edcbb8e784fdb3e253d7428d0380b877f2c484e3</cites><orcidid>0000-0002-2265-1889</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00425-022-03981-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00425-022-03981-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Lu, Xinxin</creatorcontrib><creatorcontrib>Li, Ze</creatorcontrib><creatorcontrib>Huang, Wenyue</creatorcontrib><creatorcontrib>Wang, Shaoxing</creatorcontrib><creatorcontrib>Zhang, Shifan</creatorcontrib><creatorcontrib>Li, Fei</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Sun, Rifei</creatorcontrib><creatorcontrib>Li, Guoliang</creatorcontrib><creatorcontrib>Zhang, Shujiang</creatorcontrib><title>Mapping and identification of a new potential dominant resistance gene to turnip mosaic virus in Brassica rapa</title><title>Planta</title><addtitle>Planta</addtitle><description>Main conclusion
By constructing an F
2
population, a new potential dominant resistance gene to TuMV in
Brassica rapa
was mapped and identified.
Brassica rapa
is the most widely grown vegetable crop in China, and turnip mosaic virus (TuMV) is a great threat to its production. Hence, it is a very important work to excavate more and novel resistance genes in
B. rapa
. In this study, the resistant line B80124 and the susceptible line B80450 were used to construct the F
2
populations, and through genetic analysis, the resistance to TuMV was found to be controlled by a dominant gene. Bulked segregant analysis sequence (BSA-seq) was used for the primary mapping, and an intersection (22.25–25.03 Mb) was obtained. After fine mapping using single nucleotide polymorphisms (SNP) markers, the candidate region was narrowed to 330 kb between the SNP markers A06S11 and A06S14, including eight genes relating to disease resistance. Using the transcriptome analysis and sequence identification,
BraA06g035130.3C
was screened as the final candidate gene, and it contained two deletion mutations, leading to frameshift in the susceptible line B80450. In addition, the phylogenetic analysis, hydrophilia and hydrophobicity analysis, subcellular location prediction analysis, amino acid bias analysis, and 3D modeling structures of
BraA06g035130.3C
were conducted to predict its functions. This study was conducive to the identification of a new TuMV resistance gene in
B. rapa
, which is of important scientific significance and application value for the improvement of TuMV resistance traits and molecular design breeding for Brassica crops.</description><subject>Agriculture</subject><subject>Amino acids</subject><subject>Biomedical and Life Sciences</subject><subject>Brassica</subject><subject>Brassica rapa</subject><subject>Disease resistance</subject><subject>Ecology</subject><subject>Forestry</subject><subject>Frameshift mutation</subject><subject>Gene deletion</subject><subject>Gene mapping</subject><subject>Genes</subject><subject>Genetic analysis</subject><subject>Hydrophobicity</subject><subject>Life Sciences</subject><subject>Mapping</subject><subject>Markers</subject><subject>Mutation</subject><subject>Nucleotides</subject><subject>Original Article</subject><subject>Phylogeny</subject><subject>Plant breeding</subject><subject>Plant Sciences</subject><subject>Population genetics</subject><subject>Single-nucleotide polymorphism</subject><subject>Three dimensional models</subject><subject>Transcriptomes</subject><subject>Turnips</subject><subject>Viruses</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kUtLAzEUhYMoWKt_wFXAjZvRO3lM0qUWX1Bxo-uQmWRKSpuMSUbx35u2guDCLJIL5zuHGw5C5zVc1QDiOgEwwisgpAI6k3XFD9CkZpRUBJg8RBOAMsOM8mN0ktIKoIhCTJB_1sPg_BJrb7Az1mfXu05nFzwOPdbY2088hLwV9BqbsHFe-4yjTS5l7TuLl9ZbnAPOY_RuwJuQtOvwh4tjws7j26hTKpE46kGfoqNer5M9-3mn6O3-7nX-WC1eHp7mN4uqo5zkStSmJ6zcDcwawpu-kS2ThhFruraVVkjWm5ZawqkRjEgDVEIrhehJxySzdIou97lDDO-jTVltXOrseq29DWNSRICQgnFeF_TiD7oK5Sdlux0lSFNOocie6mJIKdpeDdFtdPxSNahtBWpfgSoVqF0FihcT3ZtSgf3Sxt_of1zf7kKJkg</recordid><startdate>20221001</startdate><enddate>20221001</enddate><creator>Lu, Xinxin</creator><creator>Li, Ze</creator><creator>Huang, Wenyue</creator><creator>Wang, Shaoxing</creator><creator>Zhang, Shifan</creator><creator>Li, Fei</creator><creator>Zhang, Hui</creator><creator>Sun, Rifei</creator><creator>Li, Guoliang</creator><creator>Zhang, Shujiang</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2265-1889</orcidid></search><sort><creationdate>20221001</creationdate><title>Mapping and identification of a new potential dominant resistance gene to turnip mosaic virus in Brassica rapa</title><author>Lu, Xinxin ; Li, Ze ; Huang, Wenyue ; Wang, Shaoxing ; Zhang, Shifan ; Li, Fei ; Zhang, Hui ; Sun, Rifei ; Li, Guoliang ; Zhang, Shujiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-71df2471d6096256f68b48d42edcbb8e784fdb3e253d7428d0380b877f2c484e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Agriculture</topic><topic>Amino acids</topic><topic>Biomedical and Life Sciences</topic><topic>Brassica</topic><topic>Brassica rapa</topic><topic>Disease resistance</topic><topic>Ecology</topic><topic>Forestry</topic><topic>Frameshift mutation</topic><topic>Gene deletion</topic><topic>Gene mapping</topic><topic>Genes</topic><topic>Genetic analysis</topic><topic>Hydrophobicity</topic><topic>Life Sciences</topic><topic>Mapping</topic><topic>Markers</topic><topic>Mutation</topic><topic>Nucleotides</topic><topic>Original Article</topic><topic>Phylogeny</topic><topic>Plant breeding</topic><topic>Plant Sciences</topic><topic>Population genetics</topic><topic>Single-nucleotide polymorphism</topic><topic>Three dimensional models</topic><topic>Transcriptomes</topic><topic>Turnips</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Xinxin</creatorcontrib><creatorcontrib>Li, Ze</creatorcontrib><creatorcontrib>Huang, Wenyue</creatorcontrib><creatorcontrib>Wang, Shaoxing</creatorcontrib><creatorcontrib>Zhang, Shifan</creatorcontrib><creatorcontrib>Li, Fei</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Sun, Rifei</creatorcontrib><creatorcontrib>Li, Guoliang</creatorcontrib><creatorcontrib>Zhang, Shujiang</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Xinxin</au><au>Li, Ze</au><au>Huang, Wenyue</au><au>Wang, Shaoxing</au><au>Zhang, Shifan</au><au>Li, Fei</au><au>Zhang, Hui</au><au>Sun, Rifei</au><au>Li, Guoliang</au><au>Zhang, Shujiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mapping and identification of a new potential dominant resistance gene to turnip mosaic virus in Brassica rapa</atitle><jtitle>Planta</jtitle><stitle>Planta</stitle><date>2022-10-01</date><risdate>2022</risdate><volume>256</volume><issue>4</issue><spage>66</spage><epage>66</epage><pages>66-66</pages><artnum>66</artnum><issn>0032-0935</issn><eissn>1432-2048</eissn><abstract>Main conclusion
By constructing an F
2
population, a new potential dominant resistance gene to TuMV in
Brassica rapa
was mapped and identified.
Brassica rapa
is the most widely grown vegetable crop in China, and turnip mosaic virus (TuMV) is a great threat to its production. Hence, it is a very important work to excavate more and novel resistance genes in
B. rapa
. In this study, the resistant line B80124 and the susceptible line B80450 were used to construct the F
2
populations, and through genetic analysis, the resistance to TuMV was found to be controlled by a dominant gene. Bulked segregant analysis sequence (BSA-seq) was used for the primary mapping, and an intersection (22.25–25.03 Mb) was obtained. After fine mapping using single nucleotide polymorphisms (SNP) markers, the candidate region was narrowed to 330 kb between the SNP markers A06S11 and A06S14, including eight genes relating to disease resistance. Using the transcriptome analysis and sequence identification,
BraA06g035130.3C
was screened as the final candidate gene, and it contained two deletion mutations, leading to frameshift in the susceptible line B80450. In addition, the phylogenetic analysis, hydrophilia and hydrophobicity analysis, subcellular location prediction analysis, amino acid bias analysis, and 3D modeling structures of
BraA06g035130.3C
were conducted to predict its functions. This study was conducive to the identification of a new TuMV resistance gene in
B. rapa
, which is of important scientific significance and application value for the improvement of TuMV resistance traits and molecular design breeding for Brassica crops.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00425-022-03981-5</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-2265-1889</orcidid></addata></record> |
| fulltext | fulltext |
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| source | Springer Journals |
| subjects | Agriculture Amino acids Biomedical and Life Sciences Brassica Brassica rapa Disease resistance Ecology Forestry Frameshift mutation Gene deletion Gene mapping Genes Genetic analysis Hydrophobicity Life Sciences Mapping Markers Mutation Nucleotides Original Article Phylogeny Plant breeding Plant Sciences Population genetics Single-nucleotide polymorphism Three dimensional models Transcriptomes Turnips Viruses |
| title | Mapping and identification of a new potential dominant resistance gene to turnip mosaic virus in Brassica rapa |
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