Production of optically pure L-valine in fluidized and packed bed reactors with immobilized L-aminoacylase
A process to obtain L‐valine has been developed using fluidized and packed bed reactors with L‐aminoacylase (from hog kidney) immobilized by covalent binding. L‐Valine production using the immobilized derivative of L‐aminoacylase in fluidized and packed bed reactors was studied at three different su...
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| Veröffentlicht in: | Journal of chemical technology and biotechnology (1986) 1999-05, Vol.74 (5), p.403-408 |
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| container_title | Journal of chemical technology and biotechnology (1986) |
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| creator | Bódalo-Santoyo, A Gómez-Carrasco, J L Gómez-Gómez, E Bastida-Rodriquez, J Máximo-Martín, M F Hidalgo-Montesinos, A M |
| description | A process to obtain L‐valine has been developed using fluidized and packed bed reactors with L‐aminoacylase (from hog kidney) immobilized by covalent binding. L‐Valine production using the immobilized derivative of L‐aminoacylase in fluidized and packed bed reactors was studied at three different substrate concentrations and two different flow rates. Higher productions were obtained in the packed bed reactor in all cases. The different solubilities of L‐valine and acetyl‐D‐valine in ethanol were used to purify L‐amino acid from the reactor effluents. The amount of added ethanol did not influence the separation yields, although the purity of L‐valine was strongly affected by this parameter. The last step involved was racemization of the unhydrolyzed acetyl‐D‐valine, which was then used as substrate in a new reaction cycle.
© 1999 Society of Chemical Industry |
| doi_str_mv | 10.1002/(SICI)1097-4660(199905)74:5<403::AID-JCTB51>3.0.CO;2-N |
| format | Article |
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© 1999 Society of Chemical Industry</description><identifier>ISSN: 0268-2575</identifier><identifier>EISSN: 1097-4660</identifier><identifier>DOI: 10.1002/(SICI)1097-4660(199905)74:5<403::AID-JCTB51>3.0.CO;2-N</identifier><identifier>CODEN: JCTBDC</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Amino acids ; Bioconversions. Hemisynthesis ; Biological and medical sciences ; Bioreactors ; Biotechnology ; Chemical bonds ; Ethanol ; fluidized ; Fluidized beds ; Fundamental and applied biological sciences. Psychology ; immobilized enzyme ; L-aminoacylase ; L-valine ; Methods. Procedures. Technologies ; packed bed reactors ; Packed beds ; Purification ; Separation ; Solubility</subject><ispartof>Journal of chemical technology and biotechnology (1986), 1999-05, Vol.74 (5), p.403-408</ispartof><rights>Copyright © 1999 Society of Chemical Industry</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291097-4660%28199905%2974%3A5%3C403%3A%3AAID-JCTB51%3E3.0.CO%3B2-N$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291097-4660%28199905%2974%3A5%3C403%3A%3AAID-JCTB51%3E3.0.CO%3B2-N$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1771497$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Bódalo-Santoyo, A</creatorcontrib><creatorcontrib>Gómez-Carrasco, J L</creatorcontrib><creatorcontrib>Gómez-Gómez, E</creatorcontrib><creatorcontrib>Bastida-Rodriquez, J</creatorcontrib><creatorcontrib>Máximo-Martín, M F</creatorcontrib><creatorcontrib>Hidalgo-Montesinos, A M</creatorcontrib><title>Production of optically pure L-valine in fluidized and packed bed reactors with immobilized L-aminoacylase</title><title>Journal of chemical technology and biotechnology (1986)</title><addtitle>J. Chem. Technol. Biotechnol</addtitle><description>A process to obtain L‐valine has been developed using fluidized and packed bed reactors with L‐aminoacylase (from hog kidney) immobilized by covalent binding. L‐Valine production using the immobilized derivative of L‐aminoacylase in fluidized and packed bed reactors was studied at three different substrate concentrations and two different flow rates. Higher productions were obtained in the packed bed reactor in all cases. The different solubilities of L‐valine and acetyl‐D‐valine in ethanol were used to purify L‐amino acid from the reactor effluents. The amount of added ethanol did not influence the separation yields, although the purity of L‐valine was strongly affected by this parameter. The last step involved was racemization of the unhydrolyzed acetyl‐D‐valine, which was then used as substrate in a new reaction cycle.
© 1999 Society of Chemical Industry</description><subject>Amino acids</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Chemical bonds</subject><subject>Ethanol</subject><subject>fluidized</subject><subject>Fluidized beds</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>immobilized enzyme</subject><subject>L-aminoacylase</subject><subject>L-valine</subject><subject>Methods. Procedures. Technologies</subject><subject>packed bed reactors</subject><subject>Packed beds</subject><subject>Purification</subject><subject>Separation</subject><subject>Solubility</subject><issn>0268-2575</issn><issn>1097-4660</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkV1v0zAUQCMEEmXwH_KA0PaQcv2dFDRpCzCKSoto0Xi7chIbvOWLOGGUX09Cq_HIg2Xr-vg8-ATBOYE5AaAvT7fLdHlGIFERlxJOSZIkIM4UX4jXHNhicbF8E31Id5eCnLM5zNPNKxqtHwSz-ycPgxlQGUdUKPE4eOL9DQDImMpZcPOpa4oh711Th40Nm7Z3uS7LfdgOnQlX0U9dutqErg5tObjC_TZFqOsibHV-Ox6zcXVG533T-fDO9d9DV1VN5sq_4CrSlasbne9L7c3T4JHVpTfPjvtJ8OXd2136PlptrpbpxSr6xoGSyGYZ07KwXCWaMssLxQnksbWCMGOBxMpKAYZZqoWhLKcmo1rnXMRFATyj7CR4cfC2XfNjML7HyvnclKWuTTN4pAqUlJL_HyRMMEgm4_MjqP34O7bTde48tp2rdLdHohThiRqxrwfszpVm_-8acOqIU0acmuDUBA8ZUXEUOGbEsSIeKiJDwHSDFNfHyaiODmrne_PrXq27W5SKKYHX6yvcfvwc7663Ei_ZH6AqqI0</recordid><startdate>199905</startdate><enddate>199905</enddate><creator>Bódalo-Santoyo, A</creator><creator>Gómez-Carrasco, J L</creator><creator>Gómez-Gómez, E</creator><creator>Bastida-Rodriquez, J</creator><creator>Máximo-Martín, M F</creator><creator>Hidalgo-Montesinos, A M</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>199905</creationdate><title>Production of optically pure L-valine in fluidized and packed bed reactors with immobilized L-aminoacylase</title><author>Bódalo-Santoyo, A ; Gómez-Carrasco, J L ; Gómez-Gómez, E ; Bastida-Rodriquez, J ; Máximo-Martín, M F ; Hidalgo-Montesinos, A M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g4021-fbb3a6df479a23f4d7410c8ff513ef0187f650e3f2a5e23c2eb2aac458dd04b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino acids</topic><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Chemical bonds</topic><topic>Ethanol</topic><topic>fluidized</topic><topic>Fluidized beds</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>immobilized enzyme</topic><topic>L-aminoacylase</topic><topic>L-valine</topic><topic>Methods. Procedures. Technologies</topic><topic>packed bed reactors</topic><topic>Packed beds</topic><topic>Purification</topic><topic>Separation</topic><topic>Solubility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bódalo-Santoyo, A</creatorcontrib><creatorcontrib>Gómez-Carrasco, J L</creatorcontrib><creatorcontrib>Gómez-Gómez, E</creatorcontrib><creatorcontrib>Bastida-Rodriquez, J</creatorcontrib><creatorcontrib>Máximo-Martín, M F</creatorcontrib><creatorcontrib>Hidalgo-Montesinos, A M</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bódalo-Santoyo, A</au><au>Gómez-Carrasco, J L</au><au>Gómez-Gómez, E</au><au>Bastida-Rodriquez, J</au><au>Máximo-Martín, M F</au><au>Hidalgo-Montesinos, A M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of optically pure L-valine in fluidized and packed bed reactors with immobilized L-aminoacylase</atitle><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle><addtitle>J. Chem. Technol. Biotechnol</addtitle><date>1999-05</date><risdate>1999</risdate><volume>74</volume><issue>5</issue><spage>403</spage><epage>408</epage><pages>403-408</pages><issn>0268-2575</issn><eissn>1097-4660</eissn><coden>JCTBDC</coden><abstract>A process to obtain L‐valine has been developed using fluidized and packed bed reactors with L‐aminoacylase (from hog kidney) immobilized by covalent binding. L‐Valine production using the immobilized derivative of L‐aminoacylase in fluidized and packed bed reactors was studied at three different substrate concentrations and two different flow rates. Higher productions were obtained in the packed bed reactor in all cases. The different solubilities of L‐valine and acetyl‐D‐valine in ethanol were used to purify L‐amino acid from the reactor effluents. The amount of added ethanol did not influence the separation yields, although the purity of L‐valine was strongly affected by this parameter. The last step involved was racemization of the unhydrolyzed acetyl‐D‐valine, which was then used as substrate in a new reaction cycle.
© 1999 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/(SICI)1097-4660(199905)74:5<403::AID-JCTB51>3.0.CO;2-N</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0268-2575 |
| ispartof | Journal of chemical technology and biotechnology (1986), 1999-05, Vol.74 (5), p.403-408 |
| issn | 0268-2575 1097-4660 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_27076664 |
| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Amino acids Bioconversions. Hemisynthesis Biological and medical sciences Bioreactors Biotechnology Chemical bonds Ethanol fluidized Fluidized beds Fundamental and applied biological sciences. Psychology immobilized enzyme L-aminoacylase L-valine Methods. Procedures. Technologies packed bed reactors Packed beds Purification Separation Solubility |
| title | Production of optically pure L-valine in fluidized and packed bed reactors with immobilized L-aminoacylase |
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