Customized normothermic machine perfusion decreases ischemia–reperfusion injury compared with static cold storage in a porcine model of liver transplantation
Background Liver transplantation has been demonstrated to be the best treatment for several liver diseases, while grafts are limited. This has caused an increase in waiting lists, making it necessary to find ways to expand the number of organs available for transplantation. Normothermic perfusion (N...
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| Veröffentlicht in: | Artificial organs 2023-01, Vol.47 (1), p.148-159 |
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| creator | Riveros, Sergio Marino, Carlo Ochoa, Gabriela Soto, Dagoberto Alegría, Leyla Zenteno, Maria José San Martín, Sebastián Brañes, Alejandro Achurra, Pablo Rebolledo, Rolando |
| description | Background
Liver transplantation has been demonstrated to be the best treatment for several liver diseases, while grafts are limited. This has caused an increase in waiting lists, making it necessary to find ways to expand the number of organs available for transplantation. Normothermic perfusion (NMP) of liver grafts has been established as an alternative to static cold storage (SCS), but only a small number of perfusion machines are commercially available.
Methods
Using a customized ex situ machine perfusion, we compared the results between ex situ NMP and SCS preservation in a porcine liver transplant model.
Results
During NMP, lactate concentrations were 80% lower after the 3‐h perfusion period, compared with SCS. Bile production had a 2.5‐fold increase during the NMP period. After transplantation, aspartate transaminase (AST) and alanine transaminase (ALT) levels were 35% less in the NMP group, compared to the SCS group. In pathologic analyses of grafts after transplant, tissue oxidation did not change between groups, but the ischemia–reperfusion injury score was lower in the NMP group.
Conclusion
NMP reduced hepatocellular damage and ischemia–reperfusion injury when compared to SCS using a customized perfusion machine. This could be an alternative for low‐income countries to include machine perfusion in their therapeutic options.
This article shows the development of a customized ex situ machine perfusion, reporting reduced hepatocellular damage and ischemia‐reperfusion injury after liver transplantation when compared to SCS in a porcine model. This could be an alternative for low‐income countries to include machine perfusion in their therapeutic options. |
| doi_str_mv | 10.1111/aor.14390 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2707618212</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2707618212</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2830-13dfcf93c03edf529d5e6e10ab23db28e044c77321338389c579474ed287b12b3</originalsourceid><addsrcrecordid>eNp1kc1u1DAQxy1ERZfCgRdAlrjAIa0_4jg5Viu-pEqVKpC4RY49Yb2K4zBOqJYT79AH6LvxJHjZFiSk-jJz-M3PM_oT8oKzU57fmYl4ykvZsEdkxZVQBVdN-ZisGK9YoaryyzF5mtKWMaZLVj0hx7LKbSPYityulzTH4H-Ao2PEEOcNYPCWBmM3fgQ6AfZL8nGkDiyCSZCoT3YDwZtfP28Q_gF-3C64ozaGyWD2Xft5Q9Ns5qyzcXC5j2i-QgapoVNEu_8gRAcDjT0d_HdAOqMZ0zSYcT8Xx2fkqDdDgud39YR8fvf20_pDcXH5_uP6_KKwopas4NL1tm-kZRJcr0TjFFTAmemEdJ2ogZWl1VoKLmUt68Yq3ZS6BCdq3XHRyRPy-uCdMH5bIM1tyFfCkBeBuKRWaKYrXgsuMvrqP3QbFxzzdplSjRZKcp6pNwfKYkwJoW8n9MHgruWs3afW5tTaP6ll9uWdcekCuL_kfUwZODsA136A3cOm9vzy6qD8DeQepbE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2759725311</pqid></control><display><type>article</type><title>Customized normothermic machine perfusion decreases ischemia–reperfusion injury compared with static cold storage in a porcine model of liver transplantation</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Riveros, Sergio ; Marino, Carlo ; Ochoa, Gabriela ; Soto, Dagoberto ; Alegría, Leyla ; Zenteno, Maria José ; San Martín, Sebastián ; Brañes, Alejandro ; Achurra, Pablo ; Rebolledo, Rolando</creator><creatorcontrib>Riveros, Sergio ; Marino, Carlo ; Ochoa, Gabriela ; Soto, Dagoberto ; Alegría, Leyla ; Zenteno, Maria José ; San Martín, Sebastián ; Brañes, Alejandro ; Achurra, Pablo ; Rebolledo, Rolando</creatorcontrib><description>Background
Liver transplantation has been demonstrated to be the best treatment for several liver diseases, while grafts are limited. This has caused an increase in waiting lists, making it necessary to find ways to expand the number of organs available for transplantation. Normothermic perfusion (NMP) of liver grafts has been established as an alternative to static cold storage (SCS), but only a small number of perfusion machines are commercially available.
Methods
Using a customized ex situ machine perfusion, we compared the results between ex situ NMP and SCS preservation in a porcine liver transplant model.
Results
During NMP, lactate concentrations were 80% lower after the 3‐h perfusion period, compared with SCS. Bile production had a 2.5‐fold increase during the NMP period. After transplantation, aspartate transaminase (AST) and alanine transaminase (ALT) levels were 35% less in the NMP group, compared to the SCS group. In pathologic analyses of grafts after transplant, tissue oxidation did not change between groups, but the ischemia–reperfusion injury score was lower in the NMP group.
Conclusion
NMP reduced hepatocellular damage and ischemia–reperfusion injury when compared to SCS using a customized perfusion machine. This could be an alternative for low‐income countries to include machine perfusion in their therapeutic options.
This article shows the development of a customized ex situ machine perfusion, reporting reduced hepatocellular damage and ischemia‐reperfusion injury after liver transplantation when compared to SCS in a porcine model. This could be an alternative for low‐income countries to include machine perfusion in their therapeutic options.</description><identifier>ISSN: 0160-564X</identifier><identifier>EISSN: 1525-1594</identifier><identifier>DOI: 10.1111/aor.14390</identifier><identifier>PMID: 36007920</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Alanine ; Alanine transaminase ; Animal models ; Animals ; Aspartate transaminase ; Bile ; Cold storage ; Cryopreservation ; Customization ; graft preservation ; Grafting ; Injury prevention ; Ischemia ; Lactic acid ; Liver ; Liver - pathology ; Liver - surgery ; Liver diseases ; Liver transplantation ; Liver Transplantation - adverse effects ; Liver Transplantation - methods ; Liver transplants ; liver viability ; machine perfusion ; organ preservation ; Organ Preservation - methods ; Oxidation ; Perfusion ; Perfusion - methods ; Reperfusion ; Reperfusion Injury - etiology ; Reperfusion Injury - pathology ; Reperfusion Injury - prevention & control ; Swine ; Transplantation ; Transplants & implants</subject><ispartof>Artificial organs, 2023-01, Vol.47 (1), p.148-159</ispartof><rights>2022 International Center for Artificial Organ and Transplantation (ICAOT) and Wiley Periodicals LLC.</rights><rights>2023 International Center for Artificial Organs and Transplantation and Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2830-13dfcf93c03edf529d5e6e10ab23db28e044c77321338389c579474ed287b12b3</citedby><cites>FETCH-LOGICAL-c2830-13dfcf93c03edf529d5e6e10ab23db28e044c77321338389c579474ed287b12b3</cites><orcidid>0000-0002-8937-3043 ; 0000-0002-7350-1530 ; 0000-0001-6532-3052 ; 0000-0003-3501-4042 ; 0000-0001-6803-423X ; 0000-0002-1512-1046 ; 0000-0002-0930-4452</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faor.14390$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faor.14390$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36007920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Riveros, Sergio</creatorcontrib><creatorcontrib>Marino, Carlo</creatorcontrib><creatorcontrib>Ochoa, Gabriela</creatorcontrib><creatorcontrib>Soto, Dagoberto</creatorcontrib><creatorcontrib>Alegría, Leyla</creatorcontrib><creatorcontrib>Zenteno, Maria José</creatorcontrib><creatorcontrib>San Martín, Sebastián</creatorcontrib><creatorcontrib>Brañes, Alejandro</creatorcontrib><creatorcontrib>Achurra, Pablo</creatorcontrib><creatorcontrib>Rebolledo, Rolando</creatorcontrib><title>Customized normothermic machine perfusion decreases ischemia–reperfusion injury compared with static cold storage in a porcine model of liver transplantation</title><title>Artificial organs</title><addtitle>Artif Organs</addtitle><description>Background
Liver transplantation has been demonstrated to be the best treatment for several liver diseases, while grafts are limited. This has caused an increase in waiting lists, making it necessary to find ways to expand the number of organs available for transplantation. Normothermic perfusion (NMP) of liver grafts has been established as an alternative to static cold storage (SCS), but only a small number of perfusion machines are commercially available.
Methods
Using a customized ex situ machine perfusion, we compared the results between ex situ NMP and SCS preservation in a porcine liver transplant model.
Results
During NMP, lactate concentrations were 80% lower after the 3‐h perfusion period, compared with SCS. Bile production had a 2.5‐fold increase during the NMP period. After transplantation, aspartate transaminase (AST) and alanine transaminase (ALT) levels were 35% less in the NMP group, compared to the SCS group. In pathologic analyses of grafts after transplant, tissue oxidation did not change between groups, but the ischemia–reperfusion injury score was lower in the NMP group.
Conclusion
NMP reduced hepatocellular damage and ischemia–reperfusion injury when compared to SCS using a customized perfusion machine. This could be an alternative for low‐income countries to include machine perfusion in their therapeutic options.
This article shows the development of a customized ex situ machine perfusion, reporting reduced hepatocellular damage and ischemia‐reperfusion injury after liver transplantation when compared to SCS in a porcine model. This could be an alternative for low‐income countries to include machine perfusion in their therapeutic options.</description><subject>Alanine</subject><subject>Alanine transaminase</subject><subject>Animal models</subject><subject>Animals</subject><subject>Aspartate transaminase</subject><subject>Bile</subject><subject>Cold storage</subject><subject>Cryopreservation</subject><subject>Customization</subject><subject>graft preservation</subject><subject>Grafting</subject><subject>Injury prevention</subject><subject>Ischemia</subject><subject>Lactic acid</subject><subject>Liver</subject><subject>Liver - pathology</subject><subject>Liver - surgery</subject><subject>Liver diseases</subject><subject>Liver transplantation</subject><subject>Liver Transplantation - adverse effects</subject><subject>Liver Transplantation - methods</subject><subject>Liver transplants</subject><subject>liver viability</subject><subject>machine perfusion</subject><subject>organ preservation</subject><subject>Organ Preservation - methods</subject><subject>Oxidation</subject><subject>Perfusion</subject><subject>Perfusion - methods</subject><subject>Reperfusion</subject><subject>Reperfusion Injury - etiology</subject><subject>Reperfusion Injury - pathology</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Swine</subject><subject>Transplantation</subject><subject>Transplants & implants</subject><issn>0160-564X</issn><issn>1525-1594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1DAQxy1ERZfCgRdAlrjAIa0_4jg5Viu-pEqVKpC4RY49Yb2K4zBOqJYT79AH6LvxJHjZFiSk-jJz-M3PM_oT8oKzU57fmYl4ykvZsEdkxZVQBVdN-ZisGK9YoaryyzF5mtKWMaZLVj0hx7LKbSPYityulzTH4H-Ao2PEEOcNYPCWBmM3fgQ6AfZL8nGkDiyCSZCoT3YDwZtfP28Q_gF-3C64ozaGyWD2Xft5Q9Ns5qyzcXC5j2i-QgapoVNEu_8gRAcDjT0d_HdAOqMZ0zSYcT8Xx2fkqDdDgud39YR8fvf20_pDcXH5_uP6_KKwopas4NL1tm-kZRJcr0TjFFTAmemEdJ2ogZWl1VoKLmUt68Yq3ZS6BCdq3XHRyRPy-uCdMH5bIM1tyFfCkBeBuKRWaKYrXgsuMvrqP3QbFxzzdplSjRZKcp6pNwfKYkwJoW8n9MHgruWs3afW5tTaP6ll9uWdcekCuL_kfUwZODsA136A3cOm9vzy6qD8DeQepbE</recordid><startdate>202301</startdate><enddate>202301</enddate><creator>Riveros, Sergio</creator><creator>Marino, Carlo</creator><creator>Ochoa, Gabriela</creator><creator>Soto, Dagoberto</creator><creator>Alegría, Leyla</creator><creator>Zenteno, Maria José</creator><creator>San Martín, Sebastián</creator><creator>Brañes, Alejandro</creator><creator>Achurra, Pablo</creator><creator>Rebolledo, Rolando</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8937-3043</orcidid><orcidid>https://orcid.org/0000-0002-7350-1530</orcidid><orcidid>https://orcid.org/0000-0001-6532-3052</orcidid><orcidid>https://orcid.org/0000-0003-3501-4042</orcidid><orcidid>https://orcid.org/0000-0001-6803-423X</orcidid><orcidid>https://orcid.org/0000-0002-1512-1046</orcidid><orcidid>https://orcid.org/0000-0002-0930-4452</orcidid></search><sort><creationdate>202301</creationdate><title>Customized normothermic machine perfusion decreases ischemia–reperfusion injury compared with static cold storage in a porcine model of liver transplantation</title><author>Riveros, Sergio ; Marino, Carlo ; Ochoa, Gabriela ; Soto, Dagoberto ; Alegría, Leyla ; Zenteno, Maria José ; San Martín, Sebastián ; Brañes, Alejandro ; Achurra, Pablo ; Rebolledo, Rolando</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2830-13dfcf93c03edf529d5e6e10ab23db28e044c77321338389c579474ed287b12b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alanine</topic><topic>Alanine transaminase</topic><topic>Animal models</topic><topic>Animals</topic><topic>Aspartate transaminase</topic><topic>Bile</topic><topic>Cold storage</topic><topic>Cryopreservation</topic><topic>Customization</topic><topic>graft preservation</topic><topic>Grafting</topic><topic>Injury prevention</topic><topic>Ischemia</topic><topic>Lactic acid</topic><topic>Liver</topic><topic>Liver - pathology</topic><topic>Liver - surgery</topic><topic>Liver diseases</topic><topic>Liver transplantation</topic><topic>Liver Transplantation - adverse effects</topic><topic>Liver Transplantation - methods</topic><topic>Liver transplants</topic><topic>liver viability</topic><topic>machine perfusion</topic><topic>organ preservation</topic><topic>Organ Preservation - methods</topic><topic>Oxidation</topic><topic>Perfusion</topic><topic>Perfusion - methods</topic><topic>Reperfusion</topic><topic>Reperfusion Injury - etiology</topic><topic>Reperfusion Injury - pathology</topic><topic>Reperfusion Injury - prevention & control</topic><topic>Swine</topic><topic>Transplantation</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Riveros, Sergio</creatorcontrib><creatorcontrib>Marino, Carlo</creatorcontrib><creatorcontrib>Ochoa, Gabriela</creatorcontrib><creatorcontrib>Soto, Dagoberto</creatorcontrib><creatorcontrib>Alegría, Leyla</creatorcontrib><creatorcontrib>Zenteno, Maria José</creatorcontrib><creatorcontrib>San Martín, Sebastián</creatorcontrib><creatorcontrib>Brañes, Alejandro</creatorcontrib><creatorcontrib>Achurra, Pablo</creatorcontrib><creatorcontrib>Rebolledo, Rolando</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Artificial organs</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Riveros, Sergio</au><au>Marino, Carlo</au><au>Ochoa, Gabriela</au><au>Soto, Dagoberto</au><au>Alegría, Leyla</au><au>Zenteno, Maria José</au><au>San Martín, Sebastián</au><au>Brañes, Alejandro</au><au>Achurra, Pablo</au><au>Rebolledo, Rolando</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Customized normothermic machine perfusion decreases ischemia–reperfusion injury compared with static cold storage in a porcine model of liver transplantation</atitle><jtitle>Artificial organs</jtitle><addtitle>Artif Organs</addtitle><date>2023-01</date><risdate>2023</risdate><volume>47</volume><issue>1</issue><spage>148</spage><epage>159</epage><pages>148-159</pages><issn>0160-564X</issn><eissn>1525-1594</eissn><abstract>Background
Liver transplantation has been demonstrated to be the best treatment for several liver diseases, while grafts are limited. This has caused an increase in waiting lists, making it necessary to find ways to expand the number of organs available for transplantation. Normothermic perfusion (NMP) of liver grafts has been established as an alternative to static cold storage (SCS), but only a small number of perfusion machines are commercially available.
Methods
Using a customized ex situ machine perfusion, we compared the results between ex situ NMP and SCS preservation in a porcine liver transplant model.
Results
During NMP, lactate concentrations were 80% lower after the 3‐h perfusion period, compared with SCS. Bile production had a 2.5‐fold increase during the NMP period. After transplantation, aspartate transaminase (AST) and alanine transaminase (ALT) levels were 35% less in the NMP group, compared to the SCS group. In pathologic analyses of grafts after transplant, tissue oxidation did not change between groups, but the ischemia–reperfusion injury score was lower in the NMP group.
Conclusion
NMP reduced hepatocellular damage and ischemia–reperfusion injury when compared to SCS using a customized perfusion machine. This could be an alternative for low‐income countries to include machine perfusion in their therapeutic options.
This article shows the development of a customized ex situ machine perfusion, reporting reduced hepatocellular damage and ischemia‐reperfusion injury after liver transplantation when compared to SCS in a porcine model. This could be an alternative for low‐income countries to include machine perfusion in their therapeutic options.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>36007920</pmid><doi>10.1111/aor.14390</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-8937-3043</orcidid><orcidid>https://orcid.org/0000-0002-7350-1530</orcidid><orcidid>https://orcid.org/0000-0001-6532-3052</orcidid><orcidid>https://orcid.org/0000-0003-3501-4042</orcidid><orcidid>https://orcid.org/0000-0001-6803-423X</orcidid><orcidid>https://orcid.org/0000-0002-1512-1046</orcidid><orcidid>https://orcid.org/0000-0002-0930-4452</orcidid></addata></record> |
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| ispartof | Artificial organs, 2023-01, Vol.47 (1), p.148-159 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Alanine Alanine transaminase Animal models Animals Aspartate transaminase Bile Cold storage Cryopreservation Customization graft preservation Grafting Injury prevention Ischemia Lactic acid Liver Liver - pathology Liver - surgery Liver diseases Liver transplantation Liver Transplantation - adverse effects Liver Transplantation - methods Liver transplants liver viability machine perfusion organ preservation Organ Preservation - methods Oxidation Perfusion Perfusion - methods Reperfusion Reperfusion Injury - etiology Reperfusion Injury - pathology Reperfusion Injury - prevention & control Swine Transplantation Transplants & implants |
| title | Customized normothermic machine perfusion decreases ischemia–reperfusion injury compared with static cold storage in a porcine model of liver transplantation |
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