Direct visualization of living bacterial genotypes using CRISPR/Cas12a-circular reporter nanoprobes

Bacterial genotyping is important for understanding the complex microbiota. Although fluorescence in situ hybridization (FISH) has enabled bacterial community identification with high spatial resolution, its unavoidable cell fixation steps and signal generation by multi-probe stacking greatly limit...

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Veröffentlicht in:	Biosensors & bioelectronics 2022-11, Vol.216, p.114641-114641, Article 114641
Hauptverfasser:	Li, Yanan, Pei, Yiran, Huang, Di, Xu, Ru, Wu, Yonghua, Li, Yaqiong, Zhou, Ruixue, Wei, Yongbin, Tan, Xiao, Gao, Hua, Shi, Jinjin, Zhang, Zhenzhong, Zhang, Kaixiang, Li, Jinghong
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial genotype Bioassay Biopolymer materials CRISPR/Cas12a In vivo imaging
Online-Zugang:	Volltext
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