Direct visualization of living bacterial genotypes using CRISPR/Cas12a-circular reporter nanoprobes

Bacterial genotyping is important for understanding the complex microbiota. Although fluorescence in situ hybridization (FISH) has enabled bacterial community identification with high spatial resolution, its unavoidable cell fixation steps and signal generation by multi-probe stacking greatly limit...

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Veröffentlicht in:Biosensors & bioelectronics 2022-11, Vol.216, p.114641-114641, Article 114641
Hauptverfasser: Li, Yanan, Pei, Yiran, Huang, Di, Xu, Ru, Wu, Yonghua, Li, Yaqiong, Zhou, Ruixue, Wei, Yongbin, Tan, Xiao, Gao, Hua, Shi, Jinjin, Zhang, Zhenzhong, Zhang, Kaixiang, Li, Jinghong
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Sprache:eng
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Zusammenfassung:Bacterial genotyping is important for understanding the complex microbiota. Although fluorescence in situ hybridization (FISH) has enabled bacterial community identification with high spatial resolution, its unavoidable cell fixation steps and signal generation by multi-probe stacking greatly limit its application in living bacterial genotyping. Here, we designed polyethyleneimine-encapsulated CRISPR/Cas12a-circular reporter nanoprobes (CasCLR) for rapid and sensitive visualization of gene information in living bacteria. We found that, nanoprobe-based sequential delivery of Cas12a/crRNA and circular reporter into bacteria allowed single genomic loci to initiate trans-cleavage activity of Cas12a, thereby cleaving CLR to generate amplified fluorescent signals for imaging of target gene. Using CasCLR, we can sensitively analyze the percentage of target bacteria in co-culture experiments and directly detect pathogenic bacteria in uncultured mouse gut microbe. In addition, CasCLR has the ability to sensitively analyze specific genotype of microbial communities in vivo. This nanobiotechnology-based bacterial gene analysis is expected to advance understanding of in vivo bacterial cytogenetic information. CasCLR is designed for directly visualizing gene information in living bacteria. By sequential delivered into living bacteria, CasCLR allow specific single copy gene loci to initiate trans-cleavage activity of Cas12a, thereby cleaving CLR to generate robust fluorescent signal with high signal to noise ratio. CasCLR can be used to identify genotypes of various bacteria to sensitively analyze pathogenic bacteria in complex samples and demonstrating excellent in vivo bacterial genotyping capabilities. [Display omitted] •The designed nanoprobe can efficiently deliver CRISPR detection system into living bacteria.•Rapidly sense low-copy genomic loci in living bacteria with high fidelity.•Circular reporter (CLR) showed improved stability in bacteria.•Sensitively analyze specific genotype of microbial communities in vivo.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2022.114641