Curcumin reduces inflammation in rat apical periodontitis
Aim The aim of the study was to evaluate the effect of systemic curcumin administration on the severity of apical periodontitis (AP). Methodology Forty male Wistar rats weighing 250–280 g each, age 2.5 months, were distributed into four groups (n = 10): control untreated rats (C), control rats treat...
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| Veröffentlicht in: | International endodontic journal 2022-11, Vol.55 (11), p.1241-1251 |
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| creator | Justo, Mariana Pagliusi Cardoso, Carolina de Barros Morais Cantiga‐Silva, Cristiane Oliveira, Pedro Henrique Chaves Sivieri‐Araújo, Gustavo Azuma, Mariane Maffei Ervolino, Edilson Cintra, Luciano Tavares Angelo |
| description | Aim
The aim of the study was to evaluate the effect of systemic curcumin administration on the severity of apical periodontitis (AP).
Methodology
Forty male Wistar rats weighing 250–280 g each, age 2.5 months, were distributed into four groups (n = 10): control untreated rats (C), control rats treated with curcumin (CUR), rats with pulp exposure‐induced apical periodontitis (AP) and rats with pulp exposure‐induced apical periodontitis treated with curcumin (AP‐CUR). Curcumin treatment was administered orally once daily for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed at 30 days, and the jaws were collected and reconstructed in a programme specific for micro‐CT. The jaws were processed for analysis of the inflammatory process using haematoxylin and eosin staining and immunohistochemical assays for interleukin tumour necrosis factor alpha (TNF‐α), interleukin (Il)‐6 and Il‐1β. Tartrate‐resistant acid phosphatase (TRAP) and osteocalcin (OCN) staining were used to analyse the resorptive process on the bone surface of periapical area. Kruskal–Wallis with Dunn's test was performed for nonparametric data and anova with Tukey's test for parametric data, p .05). The levels of inflammatory cell infiltration and immunoreactivity for the proinflammatory cytokines TNF‐α, Il‐6 and Il‐1β were significantly higher in the periapical lesions of the AP group than in the AP‐CUR group (p |
| doi_str_mv | 10.1111/iej.13819 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2706719308</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2723275933</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3309-6e93b82289b6371a3c5906618f611513eff8101bbf223dd28557d8d6e59948a83</originalsourceid><addsrcrecordid>eNp10MFKxDAQBuAgCq6rB9-g4EUP3c1kNmlylLLqyoIXPYe0TSFL29SkRfbt7VpPgnMZGL4Zhp-QW6ArmGrt7GEFKEGdkQWg4CnjCs7JgsIGUyYlvyRXMR4opZwiLIjKx1COreuSYKuxtDFxXd2YtjWD811ympshMb0rTZP0Njhf-W5wg4vX5KI2TbQ3v31JPp627_lLun973uWP-7REpCoVVmEhGZOqEJiBwZIrKgTIWgBwQFvXEigURc0YVhWTnGeVrITlSm2kkbgk9_PdPvjP0cZBty6WtmlMZ_0YNcuoyEAhPdG7P_Tgx9BN302KIcu4QpzUw6zK4GMMttZ9cK0JRw1Un0LUU4j6J8TJrmf75Rp7_B_q3fZ13vgGBBVw4A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2723275933</pqid></control><display><type>article</type><title>Curcumin reduces inflammation in rat apical periodontitis</title><source>Access via Wiley Online Library</source><creator>Justo, Mariana Pagliusi ; Cardoso, Carolina de Barros Morais ; Cantiga‐Silva, Cristiane ; Oliveira, Pedro Henrique Chaves ; Sivieri‐Araújo, Gustavo ; Azuma, Mariane Maffei ; Ervolino, Edilson ; Cintra, Luciano Tavares Angelo</creator><creatorcontrib>Justo, Mariana Pagliusi ; Cardoso, Carolina de Barros Morais ; Cantiga‐Silva, Cristiane ; Oliveira, Pedro Henrique Chaves ; Sivieri‐Araújo, Gustavo ; Azuma, Mariane Maffei ; Ervolino, Edilson ; Cintra, Luciano Tavares Angelo</creatorcontrib><description>Aim
The aim of the study was to evaluate the effect of systemic curcumin administration on the severity of apical periodontitis (AP).
Methodology
Forty male Wistar rats weighing 250–280 g each, age 2.5 months, were distributed into four groups (n = 10): control untreated rats (C), control rats treated with curcumin (CUR), rats with pulp exposure‐induced apical periodontitis (AP) and rats with pulp exposure‐induced apical periodontitis treated with curcumin (AP‐CUR). Curcumin treatment was administered orally once daily for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed at 30 days, and the jaws were collected and reconstructed in a programme specific for micro‐CT. The jaws were processed for analysis of the inflammatory process using haematoxylin and eosin staining and immunohistochemical assays for interleukin tumour necrosis factor alpha (TNF‐α), interleukin (Il)‐6 and Il‐1β. Tartrate‐resistant acid phosphatase (TRAP) and osteocalcin (OCN) staining were used to analyse the resorptive process on the bone surface of periapical area. Kruskal–Wallis with Dunn's test was performed for nonparametric data and anova with Tukey's test for parametric data, p < .05.
Results
Micro‐CT revealed no statistically significant differences in bone resorption between the AP and AP‐CUR groups (p > .05). The levels of inflammatory cell infiltration and immunoreactivity for the proinflammatory cytokines TNF‐α, Il‐6 and Il‐1β were significantly higher in the periapical lesions of the AP group than in the AP‐CUR group (p < .05). The number of TRAP‐positive multinucleated cells was higher in the AP group than in the AP‐CUR group (p < .05). In OCN‐positive cells, no differences were observed between the AP and AP‐CUR groups (p > .05).
Conclusions
Oral supplementation with curcumin had a significant effect on the AP severity in rats, suggesting an anti‐inflammatory effect of curcumin on AP development.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.13819</identifier><language>eng</language><publisher>Chichester: Wiley Subscription Services, Inc</publisher><subject>Acid phosphatase ; Acid phosphatase (tartrate-resistant) ; apical periodontitis ; Bone resorption ; Curcumin ; cytokine ; Cytokines ; Dietary supplements ; endodontic infection ; Gum disease ; Immunoreactivity ; Inflammation ; Kruskal-Wallis test ; Metastases ; Oral administration ; Osteocalcin ; Periodontitis ; Rodents ; Statistical analysis ; Tumor necrosis factor ; Tumor necrosis factor-TNF ; Tumors</subject><ispartof>International endodontic journal, 2022-11, Vol.55 (11), p.1241-1251</ispartof><rights>2022 British Endodontic Society. Published by John Wiley & Sons Ltd</rights><rights>Copyright © 2022 International Endodontic Journal. Published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3309-6e93b82289b6371a3c5906618f611513eff8101bbf223dd28557d8d6e59948a83</citedby><cites>FETCH-LOGICAL-c3309-6e93b82289b6371a3c5906618f611513eff8101bbf223dd28557d8d6e59948a83</cites><orcidid>0000-0002-4943-7022 ; 0000-0002-5196-514X ; 0000-0002-8402-7408 ; 0000-0003-2348-7846 ; 0000-0003-4313-7149 ; 0000-0002-1593-7926 ; 0000-0003-4859-0583 ; 0000-0002-1426-2613</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fiej.13819$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fiej.13819$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27931,27932,45581,45582</link.rule.ids></links><search><creatorcontrib>Justo, Mariana Pagliusi</creatorcontrib><creatorcontrib>Cardoso, Carolina de Barros Morais</creatorcontrib><creatorcontrib>Cantiga‐Silva, Cristiane</creatorcontrib><creatorcontrib>Oliveira, Pedro Henrique Chaves</creatorcontrib><creatorcontrib>Sivieri‐Araújo, Gustavo</creatorcontrib><creatorcontrib>Azuma, Mariane Maffei</creatorcontrib><creatorcontrib>Ervolino, Edilson</creatorcontrib><creatorcontrib>Cintra, Luciano Tavares Angelo</creatorcontrib><title>Curcumin reduces inflammation in rat apical periodontitis</title><title>International endodontic journal</title><description>Aim
The aim of the study was to evaluate the effect of systemic curcumin administration on the severity of apical periodontitis (AP).
Methodology
Forty male Wistar rats weighing 250–280 g each, age 2.5 months, were distributed into four groups (n = 10): control untreated rats (C), control rats treated with curcumin (CUR), rats with pulp exposure‐induced apical periodontitis (AP) and rats with pulp exposure‐induced apical periodontitis treated with curcumin (AP‐CUR). Curcumin treatment was administered orally once daily for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed at 30 days, and the jaws were collected and reconstructed in a programme specific for micro‐CT. The jaws were processed for analysis of the inflammatory process using haematoxylin and eosin staining and immunohistochemical assays for interleukin tumour necrosis factor alpha (TNF‐α), interleukin (Il)‐6 and Il‐1β. Tartrate‐resistant acid phosphatase (TRAP) and osteocalcin (OCN) staining were used to analyse the resorptive process on the bone surface of periapical area. Kruskal–Wallis with Dunn's test was performed for nonparametric data and anova with Tukey's test for parametric data, p < .05.
Results
Micro‐CT revealed no statistically significant differences in bone resorption between the AP and AP‐CUR groups (p > .05). The levels of inflammatory cell infiltration and immunoreactivity for the proinflammatory cytokines TNF‐α, Il‐6 and Il‐1β were significantly higher in the periapical lesions of the AP group than in the AP‐CUR group (p < .05). The number of TRAP‐positive multinucleated cells was higher in the AP group than in the AP‐CUR group (p < .05). In OCN‐positive cells, no differences were observed between the AP and AP‐CUR groups (p > .05).
Conclusions
Oral supplementation with curcumin had a significant effect on the AP severity in rats, suggesting an anti‐inflammatory effect of curcumin on AP development.</description><subject>Acid phosphatase</subject><subject>Acid phosphatase (tartrate-resistant)</subject><subject>apical periodontitis</subject><subject>Bone resorption</subject><subject>Curcumin</subject><subject>cytokine</subject><subject>Cytokines</subject><subject>Dietary supplements</subject><subject>endodontic infection</subject><subject>Gum disease</subject><subject>Immunoreactivity</subject><subject>Inflammation</subject><subject>Kruskal-Wallis test</subject><subject>Metastases</subject><subject>Oral administration</subject><subject>Osteocalcin</subject><subject>Periodontitis</subject><subject>Rodents</subject><subject>Statistical analysis</subject><subject>Tumor necrosis factor</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumors</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp10MFKxDAQBuAgCq6rB9-g4EUP3c1kNmlylLLqyoIXPYe0TSFL29SkRfbt7VpPgnMZGL4Zhp-QW6ArmGrt7GEFKEGdkQWg4CnjCs7JgsIGUyYlvyRXMR4opZwiLIjKx1COreuSYKuxtDFxXd2YtjWD811ympshMb0rTZP0Njhf-W5wg4vX5KI2TbQ3v31JPp627_lLun973uWP-7REpCoVVmEhGZOqEJiBwZIrKgTIWgBwQFvXEigURc0YVhWTnGeVrITlSm2kkbgk9_PdPvjP0cZBty6WtmlMZ_0YNcuoyEAhPdG7P_Tgx9BN302KIcu4QpzUw6zK4GMMttZ9cK0JRw1Un0LUU4j6J8TJrmf75Rp7_B_q3fZ13vgGBBVw4A</recordid><startdate>202211</startdate><enddate>202211</enddate><creator>Justo, Mariana Pagliusi</creator><creator>Cardoso, Carolina de Barros Morais</creator><creator>Cantiga‐Silva, Cristiane</creator><creator>Oliveira, Pedro Henrique Chaves</creator><creator>Sivieri‐Araújo, Gustavo</creator><creator>Azuma, Mariane Maffei</creator><creator>Ervolino, Edilson</creator><creator>Cintra, Luciano Tavares Angelo</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4943-7022</orcidid><orcidid>https://orcid.org/0000-0002-5196-514X</orcidid><orcidid>https://orcid.org/0000-0002-8402-7408</orcidid><orcidid>https://orcid.org/0000-0003-2348-7846</orcidid><orcidid>https://orcid.org/0000-0003-4313-7149</orcidid><orcidid>https://orcid.org/0000-0002-1593-7926</orcidid><orcidid>https://orcid.org/0000-0003-4859-0583</orcidid><orcidid>https://orcid.org/0000-0002-1426-2613</orcidid></search><sort><creationdate>202211</creationdate><title>Curcumin reduces inflammation in rat apical periodontitis</title><author>Justo, Mariana Pagliusi ; Cardoso, Carolina de Barros Morais ; Cantiga‐Silva, Cristiane ; Oliveira, Pedro Henrique Chaves ; Sivieri‐Araújo, Gustavo ; Azuma, Mariane Maffei ; Ervolino, Edilson ; Cintra, Luciano Tavares Angelo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3309-6e93b82289b6371a3c5906618f611513eff8101bbf223dd28557d8d6e59948a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Acid phosphatase</topic><topic>Acid phosphatase (tartrate-resistant)</topic><topic>apical periodontitis</topic><topic>Bone resorption</topic><topic>Curcumin</topic><topic>cytokine</topic><topic>Cytokines</topic><topic>Dietary supplements</topic><topic>endodontic infection</topic><topic>Gum disease</topic><topic>Immunoreactivity</topic><topic>Inflammation</topic><topic>Kruskal-Wallis test</topic><topic>Metastases</topic><topic>Oral administration</topic><topic>Osteocalcin</topic><topic>Periodontitis</topic><topic>Rodents</topic><topic>Statistical analysis</topic><topic>Tumor necrosis factor</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Justo, Mariana Pagliusi</creatorcontrib><creatorcontrib>Cardoso, Carolina de Barros Morais</creatorcontrib><creatorcontrib>Cantiga‐Silva, Cristiane</creatorcontrib><creatorcontrib>Oliveira, Pedro Henrique Chaves</creatorcontrib><creatorcontrib>Sivieri‐Araújo, Gustavo</creatorcontrib><creatorcontrib>Azuma, Mariane Maffei</creatorcontrib><creatorcontrib>Ervolino, Edilson</creatorcontrib><creatorcontrib>Cintra, Luciano Tavares Angelo</creatorcontrib><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Justo, Mariana Pagliusi</au><au>Cardoso, Carolina de Barros Morais</au><au>Cantiga‐Silva, Cristiane</au><au>Oliveira, Pedro Henrique Chaves</au><au>Sivieri‐Araújo, Gustavo</au><au>Azuma, Mariane Maffei</au><au>Ervolino, Edilson</au><au>Cintra, Luciano Tavares Angelo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Curcumin reduces inflammation in rat apical periodontitis</atitle><jtitle>International endodontic journal</jtitle><date>2022-11</date><risdate>2022</risdate><volume>55</volume><issue>11</issue><spage>1241</spage><epage>1251</epage><pages>1241-1251</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Aim
The aim of the study was to evaluate the effect of systemic curcumin administration on the severity of apical periodontitis (AP).
Methodology
Forty male Wistar rats weighing 250–280 g each, age 2.5 months, were distributed into four groups (n = 10): control untreated rats (C), control rats treated with curcumin (CUR), rats with pulp exposure‐induced apical periodontitis (AP) and rats with pulp exposure‐induced apical periodontitis treated with curcumin (AP‐CUR). Curcumin treatment was administered orally once daily for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed at 30 days, and the jaws were collected and reconstructed in a programme specific for micro‐CT. The jaws were processed for analysis of the inflammatory process using haematoxylin and eosin staining and immunohistochemical assays for interleukin tumour necrosis factor alpha (TNF‐α), interleukin (Il)‐6 and Il‐1β. Tartrate‐resistant acid phosphatase (TRAP) and osteocalcin (OCN) staining were used to analyse the resorptive process on the bone surface of periapical area. Kruskal–Wallis with Dunn's test was performed for nonparametric data and anova with Tukey's test for parametric data, p < .05.
Results
Micro‐CT revealed no statistically significant differences in bone resorption between the AP and AP‐CUR groups (p > .05). The levels of inflammatory cell infiltration and immunoreactivity for the proinflammatory cytokines TNF‐α, Il‐6 and Il‐1β were significantly higher in the periapical lesions of the AP group than in the AP‐CUR group (p < .05). The number of TRAP‐positive multinucleated cells was higher in the AP group than in the AP‐CUR group (p < .05). In OCN‐positive cells, no differences were observed between the AP and AP‐CUR groups (p > .05).
Conclusions
Oral supplementation with curcumin had a significant effect on the AP severity in rats, suggesting an anti‐inflammatory effect of curcumin on AP development.</abstract><cop>Chichester</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/iej.13819</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4943-7022</orcidid><orcidid>https://orcid.org/0000-0002-5196-514X</orcidid><orcidid>https://orcid.org/0000-0002-8402-7408</orcidid><orcidid>https://orcid.org/0000-0003-2348-7846</orcidid><orcidid>https://orcid.org/0000-0003-4313-7149</orcidid><orcidid>https://orcid.org/0000-0002-1593-7926</orcidid><orcidid>https://orcid.org/0000-0003-4859-0583</orcidid><orcidid>https://orcid.org/0000-0002-1426-2613</orcidid></addata></record> |
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| subjects | Acid phosphatase Acid phosphatase (tartrate-resistant) apical periodontitis Bone resorption Curcumin cytokine Cytokines Dietary supplements endodontic infection Gum disease Immunoreactivity Inflammation Kruskal-Wallis test Metastases Oral administration Osteocalcin Periodontitis Rodents Statistical analysis Tumor necrosis factor Tumor necrosis factor-TNF Tumors |
| title | Curcumin reduces inflammation in rat apical periodontitis |
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