Extracellular vesicles from bone marrow mesenchymal stromal cells of severe aplastic anemia patients attenuate hematopoietic functions of CD34+ hematopoietic stem and progenitor cells
Mesenchymal stromal cells (MSC) regulate hematopoiesis in the bone marrow (BM) niche and extracellular vesicles (EVs) released by BM‐MSC are important mediators of the cross‐talk between BM‐MSC and hematopoietic stem and progenitor cells (HSPC). We have previously demonstrated that BM‐MSC of severe...
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| Veröffentlicht in: | Cell biology international 2022-11, Vol.46 (11), p.1970-1976 |
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| container_end_page | 1976 |
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| container_issue | 11 |
| container_start_page | 1970 |
| container_title | Cell biology international |
| container_volume | 46 |
| creator | Srivastava, Jyotika Katiyar, Shobhita Chaturvedi, Chandra P. Nityanand, Soniya |
| description | Mesenchymal stromal cells (MSC) regulate hematopoiesis in the bone marrow (BM) niche and extracellular vesicles (EVs) released by BM‐MSC are important mediators of the cross‐talk between BM‐MSC and hematopoietic stem and progenitor cells (HSPC). We have previously demonstrated that BM‐MSC of severe aplastic anemia (SAA) patients have an altered expression of hematopoiesis regulatory molecules. In the present study, we observed that CD34+ HSPC when cocultured with BM‐MSC EVs from aplastic anemia patients exhibited a significant reduction in colony‐forming units (p = .001), cell proliferation (p = .002), and increased apoptosis (p > .001) when compared to coculture with BM‐MSC EVs from controls. Collectively, our results highlight that EVs derived from the BM‐MSC of SAA patients impair the hematopoiesis supporting function of HSPC. |
| doi_str_mv | 10.1002/cbin.11885 |
| format | Article |
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We have previously demonstrated that BM‐MSC of severe aplastic anemia (SAA) patients have an altered expression of hematopoiesis regulatory molecules. In the present study, we observed that CD34+ HSPC when cocultured with BM‐MSC EVs from aplastic anemia patients exhibited a significant reduction in colony‐forming units (p = .001), cell proliferation (p = .002), and increased apoptosis (p > .001) when compared to coculture with BM‐MSC EVs from controls. 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Collectively, our results highlight that EVs derived from the BM‐MSC of SAA patients impair the hematopoiesis supporting function of HSPC.</description><subject>Anemia</subject><subject>Aplastic anemia</subject><subject>Apoptosis</subject><subject>Bone marrow</subject><subject>bone marrow mesenchymal stromal cells</subject><subject>CD34 antigen</subject><subject>Cell proliferation</subject><subject>colony forming units</subject><subject>Extracellular vesicles</subject><subject>Hematopoietic stem cells</subject><subject>hematopoisis</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchyme</subject><subject>Progenitor cells</subject><subject>Stromal cells</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kcFu1TAQRSMEEqWw4QsssUGgFDu285IlPApUqtoNrKOJPaGuEjt4nJb3N_2MrvkynIYNLFiNpTlz545vUbwU_ERwXr0zvfMnQjSNflQcCd7qspFaP17ftS7rttVPi2dE15wLoZr6qLg__ZkiGBzHZYTIbpCcGZHYEMPE-uCRTRBjuGUTEnpzdZhgZJRyN9d1jFgYGOENRmQwj0DJGQYeJwdshuTQJ2KQEvoFErIrnCCFOThcuWHxJrngH0T2H6V6--vub4ISTlnOsjmG7-hdCnFb-7x4MsBI-OJPPS6-fTr9uv9Snl9-Ptu_Py-NlEqXUAkcatG3tuWqqWqL_c5wazUqga0EBda0CoWClnNUSlhph11vJIDQGqw8Ll5vutnAjwUpdZOj1UG-MSzUVTtei6YSuyajr_5Br8MSfXaXqUpKzTnXmXqzUSYGoohDN0eXP_nQCd6tIXZriN1DiBkWG3zrRjz8h-z2H84utpnfkaClOg</recordid><startdate>202211</startdate><enddate>202211</enddate><creator>Srivastava, Jyotika</creator><creator>Katiyar, Shobhita</creator><creator>Chaturvedi, Chandra P.</creator><creator>Nityanand, Soniya</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6031-6272</orcidid></search><sort><creationdate>202211</creationdate><title>Extracellular vesicles from bone marrow mesenchymal stromal cells of severe aplastic anemia patients attenuate hematopoietic functions of CD34+ hematopoietic stem and progenitor cells</title><author>Srivastava, Jyotika ; Katiyar, Shobhita ; Chaturvedi, Chandra P. ; Nityanand, Soniya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3345-a21ef61b9d904826deb7c0dd5e41e93a4adc94e14a900e441d3df7bc3aa155ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Anemia</topic><topic>Aplastic anemia</topic><topic>Apoptosis</topic><topic>Bone marrow</topic><topic>bone marrow mesenchymal stromal cells</topic><topic>CD34 antigen</topic><topic>Cell proliferation</topic><topic>colony forming units</topic><topic>Extracellular vesicles</topic><topic>Hematopoietic stem cells</topic><topic>hematopoisis</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchyme</topic><topic>Progenitor cells</topic><topic>Stromal cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Srivastava, Jyotika</creatorcontrib><creatorcontrib>Katiyar, Shobhita</creatorcontrib><creatorcontrib>Chaturvedi, Chandra P.</creatorcontrib><creatorcontrib>Nityanand, Soniya</creatorcontrib><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Srivastava, Jyotika</au><au>Katiyar, Shobhita</au><au>Chaturvedi, Chandra P.</au><au>Nityanand, Soniya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extracellular vesicles from bone marrow mesenchymal stromal cells of severe aplastic anemia patients attenuate hematopoietic functions of CD34+ hematopoietic stem and progenitor cells</atitle><jtitle>Cell biology international</jtitle><date>2022-11</date><risdate>2022</risdate><volume>46</volume><issue>11</issue><spage>1970</spage><epage>1976</epage><pages>1970-1976</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>Mesenchymal stromal cells (MSC) regulate hematopoiesis in the bone marrow (BM) niche and extracellular vesicles (EVs) released by BM‐MSC are important mediators of the cross‐talk between BM‐MSC and hematopoietic stem and progenitor cells (HSPC). We have previously demonstrated that BM‐MSC of severe aplastic anemia (SAA) patients have an altered expression of hematopoiesis regulatory molecules. In the present study, we observed that CD34+ HSPC when cocultured with BM‐MSC EVs from aplastic anemia patients exhibited a significant reduction in colony‐forming units (p = .001), cell proliferation (p = .002), and increased apoptosis (p > .001) when compared to coculture with BM‐MSC EVs from controls. Collectively, our results highlight that EVs derived from the BM‐MSC of SAA patients impair the hematopoiesis supporting function of HSPC.</abstract><cop>London</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/cbin.11885</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-6031-6272</orcidid></addata></record> |
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| ispartof | Cell biology international, 2022-11, Vol.46 (11), p.1970-1976 |
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| subjects | Anemia Aplastic anemia Apoptosis Bone marrow bone marrow mesenchymal stromal cells CD34 antigen Cell proliferation colony forming units Extracellular vesicles Hematopoietic stem cells hematopoisis Mesenchymal stem cells Mesenchyme Progenitor cells Stromal cells |
| title | Extracellular vesicles from bone marrow mesenchymal stromal cells of severe aplastic anemia patients attenuate hematopoietic functions of CD34+ hematopoietic stem and progenitor cells |
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