Pourbiax Diagrams as an Aid to Understanding the Impact of Acid/Base Disturbance on Blood Glucose Point-of-Care Testing
Abstract Objective Assays based on redox reactions that involve proton transfer are vulnerable to artifactual findings in metabolic acidosis/alkalosis. We evaluated the impact of pH on the measurement of blood glucose by the glucose dehydrogenase/pyrroloquinoline quinone system used in point-of-care...
Gespeichert in:
| Veröffentlicht in: | Laboratory medicine 2023-01, Vol.54 (1), p.72-74 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 74 |
|---|---|
| container_issue | 1 |
| container_start_page | 72 |
| container_title | Laboratory medicine |
| container_volume | 54 |
| creator | McPherson, Peter A C McClements, Owen S Johnston, Ben M |
| description | Abstract
Objective
Assays based on redox reactions that involve proton transfer are vulnerable to artifactual findings in metabolic acidosis/alkalosis. We evaluated the impact of pH on the measurement of blood glucose by the glucose dehydrogenase/pyrroloquinoline quinone system used in point-of-care-testing.
Methods
We applied a series of thermodynamic equations to adjust the Gibbs energy for the pyrroloquinoline quinone couple. This adjusts values taken under standard conditions to those more closely resembling the physiological state.
Results
Under standard conditions, the pyrroloquinoline quinone couple has Eo = −0.125 V whereas adjustment to the physiological state (pH 7.40, ionic strength 0.15 mol/L, and temperature 310.15°K) yields Eo′ = −0.166 V. This corresponds to an uncertainty in blood glucose determination of approximately 0.13 mmol/L.
Conclusion
We have demonstrated that the impact of pH on blood glucose determination by the glucose dehydrogenase/pyrroloquinoline quinone system (under physiologically relevant conditions of ionic strength and temperature) is not clinically significant. |
| doi_str_mv | 10.1093/labmed/lmac069 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2703416605</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A776585348</galeid><oup_id>10.1093/labmed/lmac069</oup_id><sourcerecordid>A776585348</sourcerecordid><originalsourceid>FETCH-LOGICAL-c379t-d19b87a695313d76503a1df09aa80970cf24c0b55e978c3a0a2a913da2caac23</originalsourceid><addsrcrecordid>eNqFkcFvFCEUxomxsWv16tGQeKmH6TIwwHDcrlqbNGkP65m8BWalmYEVmGj_e6m7jYlpYiAh7_H7Pl74EHrXkouWKLYcYTs5uxwnMESoF2jRqo41UjLyEi0IIbLhhMpT9Drn-1p2StBX6JRxJYWibIF-3sU5bT38wp887BJMGUPdAa-8xSXib8G6lAsE68MOl-8OX097MAXHAa-Mt8tLyK5qc6k2EIzDMeDLMUaLr8bZxHp5F30oTRyaNSSHNy6XavUGnQwwZvf2eJ6hzZfPm_XX5ub26nq9umkMk6o0tlXbXoJQnLXMSsEJg9YORAH0REliBtoZsuXcKdkbBgQoqEoCNQCGsjN0frDdp_hjrk_ryWfjxhGCi3PWVBLWtUIQXtEP_6D39WtCHU7TnrKeCyLkX2oHo9M-DLEkMI-meiXrfD1nXV-pi2eouqybvInBDb72nxOYFHNObtD75CdID7ol-jFofQhaH4OugvfHaec__Sf8KdkKfDwAcd7_z-w3_AGxyw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2823856067</pqid></control><display><type>article</type><title>Pourbiax Diagrams as an Aid to Understanding the Impact of Acid/Base Disturbance on Blood Glucose Point-of-Care Testing</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>McPherson, Peter A C ; McClements, Owen S ; Johnston, Ben M</creator><creatorcontrib>McPherson, Peter A C ; McClements, Owen S ; Johnston, Ben M</creatorcontrib><description>Abstract
Objective
Assays based on redox reactions that involve proton transfer are vulnerable to artifactual findings in metabolic acidosis/alkalosis. We evaluated the impact of pH on the measurement of blood glucose by the glucose dehydrogenase/pyrroloquinoline quinone system used in point-of-care-testing.
Methods
We applied a series of thermodynamic equations to adjust the Gibbs energy for the pyrroloquinoline quinone couple. This adjusts values taken under standard conditions to those more closely resembling the physiological state.
Results
Under standard conditions, the pyrroloquinoline quinone couple has Eo = −0.125 V whereas adjustment to the physiological state (pH 7.40, ionic strength 0.15 mol/L, and temperature 310.15°K) yields Eo′ = −0.166 V. This corresponds to an uncertainty in blood glucose determination of approximately 0.13 mmol/L.
Conclusion
We have demonstrated that the impact of pH on blood glucose determination by the glucose dehydrogenase/pyrroloquinoline quinone system (under physiologically relevant conditions of ionic strength and temperature) is not clinically significant.</description><identifier>ISSN: 0007-5027</identifier><identifier>EISSN: 1943-7730</identifier><identifier>DOI: 10.1093/labmed/lmac069</identifier><identifier>PMID: 35976923</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><subject>Acidosis ; Alkalosis ; Aqueous solutions ; Blood Glucose ; Blood sugar ; Body temperature ; Dehydrogenases ; Glucose ; Glucose Dehydrogenases - metabolism ; Humans ; Ions ; Medical laboratories ; Metabolism ; Oxidation-Reduction ; Physiological aspects ; Physiology ; Point of care testing ; PQQ Cofactor - metabolism ; Quinone ; Thermodynamics</subject><ispartof>Laboratory medicine, 2023-01, Vol.54 (1), p.72-74</ispartof><rights>The Author(s) 2022. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2022</rights><rights>The Author(s) 2022. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><rights>COPYRIGHT 2023 Oxford University Press</rights><rights>The Author(s) 2022. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c379t-d19b87a695313d76503a1df09aa80970cf24c0b55e978c3a0a2a913da2caac23</cites><orcidid>0000-0001-6961-1158</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35976923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McPherson, Peter A C</creatorcontrib><creatorcontrib>McClements, Owen S</creatorcontrib><creatorcontrib>Johnston, Ben M</creatorcontrib><title>Pourbiax Diagrams as an Aid to Understanding the Impact of Acid/Base Disturbance on Blood Glucose Point-of-Care Testing</title><title>Laboratory medicine</title><addtitle>Lab Med</addtitle><description>Abstract
Objective
Assays based on redox reactions that involve proton transfer are vulnerable to artifactual findings in metabolic acidosis/alkalosis. We evaluated the impact of pH on the measurement of blood glucose by the glucose dehydrogenase/pyrroloquinoline quinone system used in point-of-care-testing.
Methods
We applied a series of thermodynamic equations to adjust the Gibbs energy for the pyrroloquinoline quinone couple. This adjusts values taken under standard conditions to those more closely resembling the physiological state.
Results
Under standard conditions, the pyrroloquinoline quinone couple has Eo = −0.125 V whereas adjustment to the physiological state (pH 7.40, ionic strength 0.15 mol/L, and temperature 310.15°K) yields Eo′ = −0.166 V. This corresponds to an uncertainty in blood glucose determination of approximately 0.13 mmol/L.
Conclusion
We have demonstrated that the impact of pH on blood glucose determination by the glucose dehydrogenase/pyrroloquinoline quinone system (under physiologically relevant conditions of ionic strength and temperature) is not clinically significant.</description><subject>Acidosis</subject><subject>Alkalosis</subject><subject>Aqueous solutions</subject><subject>Blood Glucose</subject><subject>Blood sugar</subject><subject>Body temperature</subject><subject>Dehydrogenases</subject><subject>Glucose</subject><subject>Glucose Dehydrogenases - metabolism</subject><subject>Humans</subject><subject>Ions</subject><subject>Medical laboratories</subject><subject>Metabolism</subject><subject>Oxidation-Reduction</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Point of care testing</subject><subject>PQQ Cofactor - metabolism</subject><subject>Quinone</subject><subject>Thermodynamics</subject><issn>0007-5027</issn><issn>1943-7730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkcFvFCEUxomxsWv16tGQeKmH6TIwwHDcrlqbNGkP65m8BWalmYEVmGj_e6m7jYlpYiAh7_H7Pl74EHrXkouWKLYcYTs5uxwnMESoF2jRqo41UjLyEi0IIbLhhMpT9Drn-1p2StBX6JRxJYWibIF-3sU5bT38wp887BJMGUPdAa-8xSXib8G6lAsE68MOl-8OX097MAXHAa-Mt8tLyK5qc6k2EIzDMeDLMUaLr8bZxHp5F30oTRyaNSSHNy6XavUGnQwwZvf2eJ6hzZfPm_XX5ub26nq9umkMk6o0tlXbXoJQnLXMSsEJg9YORAH0REliBtoZsuXcKdkbBgQoqEoCNQCGsjN0frDdp_hjrk_ryWfjxhGCi3PWVBLWtUIQXtEP_6D39WtCHU7TnrKeCyLkX2oHo9M-DLEkMI-meiXrfD1nXV-pi2eouqybvInBDb72nxOYFHNObtD75CdID7ol-jFofQhaH4OugvfHaec__Sf8KdkKfDwAcd7_z-w3_AGxyw</recordid><startdate>20230105</startdate><enddate>20230105</enddate><creator>McPherson, Peter A C</creator><creator>McClements, Owen S</creator><creator>Johnston, Ben M</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6961-1158</orcidid></search><sort><creationdate>20230105</creationdate><title>Pourbiax Diagrams as an Aid to Understanding the Impact of Acid/Base Disturbance on Blood Glucose Point-of-Care Testing</title><author>McPherson, Peter A C ; McClements, Owen S ; Johnston, Ben M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-d19b87a695313d76503a1df09aa80970cf24c0b55e978c3a0a2a913da2caac23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Acidosis</topic><topic>Alkalosis</topic><topic>Aqueous solutions</topic><topic>Blood Glucose</topic><topic>Blood sugar</topic><topic>Body temperature</topic><topic>Dehydrogenases</topic><topic>Glucose</topic><topic>Glucose Dehydrogenases - metabolism</topic><topic>Humans</topic><topic>Ions</topic><topic>Medical laboratories</topic><topic>Metabolism</topic><topic>Oxidation-Reduction</topic><topic>Physiological aspects</topic><topic>Physiology</topic><topic>Point of care testing</topic><topic>PQQ Cofactor - metabolism</topic><topic>Quinone</topic><topic>Thermodynamics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McPherson, Peter A C</creatorcontrib><creatorcontrib>McClements, Owen S</creatorcontrib><creatorcontrib>Johnston, Ben M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McPherson, Peter A C</au><au>McClements, Owen S</au><au>Johnston, Ben M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pourbiax Diagrams as an Aid to Understanding the Impact of Acid/Base Disturbance on Blood Glucose Point-of-Care Testing</atitle><jtitle>Laboratory medicine</jtitle><addtitle>Lab Med</addtitle><date>2023-01-05</date><risdate>2023</risdate><volume>54</volume><issue>1</issue><spage>72</spage><epage>74</epage><pages>72-74</pages><issn>0007-5027</issn><eissn>1943-7730</eissn><abstract>Abstract
Objective
Assays based on redox reactions that involve proton transfer are vulnerable to artifactual findings in metabolic acidosis/alkalosis. We evaluated the impact of pH on the measurement of blood glucose by the glucose dehydrogenase/pyrroloquinoline quinone system used in point-of-care-testing.
Methods
We applied a series of thermodynamic equations to adjust the Gibbs energy for the pyrroloquinoline quinone couple. This adjusts values taken under standard conditions to those more closely resembling the physiological state.
Results
Under standard conditions, the pyrroloquinoline quinone couple has Eo = −0.125 V whereas adjustment to the physiological state (pH 7.40, ionic strength 0.15 mol/L, and temperature 310.15°K) yields Eo′ = −0.166 V. This corresponds to an uncertainty in blood glucose determination of approximately 0.13 mmol/L.
Conclusion
We have demonstrated that the impact of pH on blood glucose determination by the glucose dehydrogenase/pyrroloquinoline quinone system (under physiologically relevant conditions of ionic strength and temperature) is not clinically significant.</abstract><cop>US</cop><pub>Oxford University Press</pub><pmid>35976923</pmid><doi>10.1093/labmed/lmac069</doi><tpages>3</tpages><orcidid>https://orcid.org/0000-0001-6961-1158</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-5027 |
| ispartof | Laboratory medicine, 2023-01, Vol.54 (1), p.72-74 |
| issn | 0007-5027 1943-7730 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2703416605 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Acidosis Alkalosis Aqueous solutions Blood Glucose Blood sugar Body temperature Dehydrogenases Glucose Glucose Dehydrogenases - metabolism Humans Ions Medical laboratories Metabolism Oxidation-Reduction Physiological aspects Physiology Point of care testing PQQ Cofactor - metabolism Quinone Thermodynamics |
| title | Pourbiax Diagrams as an Aid to Understanding the Impact of Acid/Base Disturbance on Blood Glucose Point-of-Care Testing |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T17%3A13%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pourbiax%20Diagrams%20as%20an%20Aid%20to%20Understanding%20the%20Impact%20of%20Acid/Base%20Disturbance%20on%20Blood%20Glucose%20Point-of-Care%20Testing&rft.jtitle=Laboratory%20medicine&rft.au=McPherson,%20Peter%20A%20C&rft.date=2023-01-05&rft.volume=54&rft.issue=1&rft.spage=72&rft.epage=74&rft.pages=72-74&rft.issn=0007-5027&rft.eissn=1943-7730&rft_id=info:doi/10.1093/labmed/lmac069&rft_dat=%3Cgale_proqu%3EA776585348%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2823856067&rft_id=info:pmid/35976923&rft_galeid=A776585348&rft_oup_id=10.1093/labmed/lmac069&rfr_iscdi=true |