Design of a novel switchable antibody display system in Pichia pastoris

Yeast surface display (YSD) has been shown to represent a powerful tool in the field of antibody discovery and engineering as well as for selection of high producer clones. However, YSD is predominantly applied in Saccharomyces cerevisiae , whereas expression of heterologous proteins is generally fa...

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Veröffentlicht in:	Applied microbiology and biotechnology 2022-09, Vol.106 (18), p.6209-6224
Hauptverfasser:	Gätjen, Dominic, Tomszak, Florian, Dettmann, Johann-Christoph, Droste, Miriam, Nölle, Volker, Wieczorek, Marek
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Antibodies Antigens Autonomously replicating sequence Binding Biomedical and Life Sciences Biotechnology Cell walls Design of experiments Display devices Expression vectors Flow cytometry Flow stability Formaldehyde dehydrogenase Fractions Fragments Genetic aspects Genetic engineering Identification and classification Invertase Life Sciences Methods and Protocols Methylotrophs Microbial Genetics and Genomics Microbiology Pichia pastoris Plasmids Proteins Saccharomyces cerevisiae Stability analysis Viral antibodies Yeast Yeasts
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